Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,267,810	T→G	33.6%	L171V (TTG→GTG)	eamA →	putative amino‑acid metabolite efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,267,810	0	T	G	33.6%	45.9 / 9.9	48	L171V (TTG→GTG)	eamA	putative amino‑acid metabolite efflux pump
Reads supporting (aligned to +/- strand):  ref base T (6/25);  new base G (15/2);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 6.61e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTGGCACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCCTGGCGTATTTGGC  >  ACB122/3267676‑3267943                                                                                                                                       |                                                                                                                                      gccTGGCACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGCCCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGt                                                                                                                                       >  6:414513/1‑135 (MQ=255)       cACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGt                                                                                                                                   <  7:61930/133‑1 (MQ=255)           gATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTTGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTg                                                                                                                             <  8:261410/135‑1 (MQ=255)               ggCAGCGAAGACGGCGGCCATGTGCCCATTCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGAGGTGGGGCCTGGGCAACATCATTACACGACGGTTCGGCTCGGTCGCCCTGGTCGCGGTGGTGATCTgggtg                                                                                                                         >  6:307321/1‑133 (MQ=255)               ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGCCCTGGTCGCGTGGGTGATCTggggg                                                                                                                         >  7:374349/1‑134 (MQ=255)               ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGTTCTTGGGt                                                                                                                         >  6:185181/1‑135 (MQ=255)               ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGGGTTGTggggg                                                                                                                         >  4:24319/1‑134 (MQ=255)               ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGGGATCGggggg                                                                                                                         >  3:459863/1‑134 (MQ=255)               ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGGGGGGTTCTggggg                                                                                                                         >  6:324116/1‑134 (MQ=255)               ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCATGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTg                                                                                                                             >  7:47464/1‑131 (MQ=255)               ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCATGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTg                                                                                                                             <  8:47464/131‑1 (MQ=255)                    cGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTTCTCTGGGGTGGGCt                                                                                                                    >  7:281578/1‑135 (MQ=255)                    cGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGGCt                                                                                                                    >  7:77012/1‑135 (MQ=255)                         aCGGCGGCCATGTGCCCATGCTGGCGTTGGGGCTGACCCTGTGTGGCGGGGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGGCGCGTTGGTGATCTGGGGTGGGCTGATAc                                                                                                               <  7:299764/135‑1 (MQ=255)                                        caaTGCTGGCGTTGGTGCTGACCCTGTTTGGCTGGGAGATGTGGGGCATGGGCAACATCATTACCCGACGGTTCGGCTCGTTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTcc                                                                                                <  2:266033/133‑1 (MQ=255)                                          ttGCTGGCGTTGGTGCTGACCGTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                              <  3:24319/134‑1 (MQ=255)                                          ctGCTGGCGTTGGTGCTGCGCCTGTGGGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGCGGCGGGCGCCCTGGTCGCGTTGGTGATCTGGGGGGGGCTGATACCGCCACTTCCATTCCTg                                                                                              <  2:267438/134‑1 (MQ=255)                                          aTGGTGGCGTTGGGGCTGACCCTGTGGGGGGGGGCGATGGGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                              <  5:324116/135‑1 (MQ=255)                                          aTGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                              <  1:4464/135‑1 (MQ=255)                                          aTGCTGGCGTTGGGGCTGACCCTGTGTGGCGGTGCGATGGGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                              <  6:419432/135‑1 (MQ=255)                                          aTGCTGGCGTGGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                              <  4:183230/135‑1 (MQ=255)                                          aTGCTGGCGTGGGTGCTGACCCTGTGGGGGGGGGCGATGGGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                              <  6:40821/135‑1 (MQ=255)                                                   ttGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGGGAGTTGGGGTGGGCGGATAACCCCCCCTCCATTACCGGCACGGt                                                                                       >  3:413405/1‑133 (MQ=255)                                                                    tgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGTTATGGGGTGGGCTGGTACCCCCACATACATTCCACGCCCAGTGCTGCTGGCGGGGATGccc                                                                    >  7:351708/1‑135 (MQ=255)                                                                    tgGCGGGGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGGGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGccc                                                                    <  5:414513/135‑1 (MQ=255)                                                                         gTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAgc                                                               <  1:309727/135‑1 (MQ=255)                                                                               tgtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTg                                                         <  5:458069/135‑1 (MQ=255)                                                                               tgtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGGGATCTGGGGTGGGCTGATACCGCCCCGTGCATTCCGGGCACTGTGCTGGTGGCTGGAAGGCCCCGCGCGCAttt                                                         >  8:83474/1‑134 (MQ=255)                                                                               tgtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGGGGGGGTCGGGGGGGGGGGGGTAACCCCCCATCCCTTCCTGGGCCGGTGCGGGGGGGGGGGAGGCCCCGCGCGCCTTg                                                         >  3:77254/1‑135 (MQ=255)                                                                               tgtgGGGCCGGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTg                                                         <  4:413398/135‑1 (MQ=255)                                                                                    ggCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAt                                                    <  5:157534/135‑1 (MQ=255)                                                                                    ggCCGGGGCAACATCATTCCCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTGGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAt                                                    <  1:425001/135‑1 (MQ=255)                                                                                     gCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGAGCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATg                                                   <  6:47686/135‑1 (MQ=255)                                                                                       cTGGGCAACATCATTACCCGACGGTGCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGcc                                                 <  6:203516/135‑1 (MQ=255)                                                                                           gCAACTTCATTACCCGACGGTTCGGCTCGGGCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTgg                                             <  8:351708/135‑1 (MQ=255)                                                                                            cAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGTTGTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGc                                            >  3:326675/1‑135 (MQ=255)                                                                                             aaCATCAGTACCCGACGGTTCGGCGCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGcc                                           <  6:301473/135‑1 (MQ=255)                                                                                                  catTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTTGTGGTATGGGGTGGGGTGATAACGCACCGTCCATTACTGGCCCTGTCCTGGTGGGTGGAAGGCCCCGCGCGCATTGGGCCTGCCTTGGCCAata                                       >  8:230553/1‑134 (MQ=255)                                                                                                        ccGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGGTCTGGGGGGGGCGGATACCGCCCCTTGCATTCCTGGCACTGTCCTGGTGGCGGGGAGGGCCCGCGCGCATTTGCCAATCCGTGGGCAATATAATTTg                                >  8:96453/1‑135 (MQ=255)                                                                                                             ggTTCGGCTCGGTCGACCTGGTCGCGTTGGGGGTCTGGGGTGGGGGGATACCGCCCCTTGCATTGCAGGCCCTGTCCTGGTGGGTGGGAGGCCCCGAGCGCCTTTGCCATGGCTTGGCCCATATCAGTTGGAGtt                           >  8:306677/1‑135 (MQ=255)                                                                                                               ttCGGCTCGGTCGACCTGGTCGCGGTGGTGATCGTGGGTGGGGTGATACCGCCCCGTCCAATACTGGCCCGGTCCAGGGGGGGGGTAGGCCCCGAGCGCCATTGCCAAGCCTTGGCCAATATAATTTGGTGttag                         >  2:83235/1‑133 (MQ=255)                                                                                                                tCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCgg                        <  3:6197/135‑1 (MQ=255)                                                                                                                        gTCGACCTGGGCGCGTTGGAGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGcc                <  1:178909/135‑1 (MQ=255)                                                                                                                         tCGACCTGGTCGCGTTGTTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc               <  7:153404/135‑1 (MQ=255)                                                                                                                         tCGACCGGGTCGCGGTGGTGAGCTGGGGGGGGCTGCTACAGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc               <  5:307321/135‑1 (MQ=255)                                                                                                                            aCCTGGTCGCGGTGGGGTTGTGGGGTGGGCTGATACCGCCCCGTCCATTCCTGGCCCGGTCCTGGTGGCTGGGAGGCCCCGAGCGCATTTGCCAAGCCGTGGCCAATATCAGTTGGAGTTCGGTGCGGGGCCggg            >  6:156303/1‑135 (MQ=255)                                                                                                                                  tCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCCTGGCGTa        <  1:40926/133‑1 (MQ=255)                                                                                                                                    gcgGTGGGGATCTGGGGTGGGCTGATACCGCCACGTCCATTACTGGCCCTGTCCTGGTGGCTGGAAGGCCCCGCGCGCAATGGCCATGCCGGGGCCCATATCAGTTGGCGTTCGGTGCTGGCCCTGGCGGCTTTg    >  8:389888/1‑135 (MQ=255)                                                                                                                                      gTTGGTGATCTGGGGTGGGCGGATAACGCCACATCCATTCCTTGCACTGTCATGGTGGCTGGAAGGCCACGCGCGCATTTGACATGCCTTTGCCAATATCACTTGGAGTTAGGTGGTGGCCCTTGCGTAtttttc  >  6:262120/1‑132 (MQ=255)                                                                                                                                       |                                                                                                                                      GCCTGGCACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCCTGGCGTATTTGGC  >  ACB122/3267676‑3267943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑