Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,300,105 A→C 30.4% L184R (CTC→CGC)  glaR ← HTH‑type transcriptional repressor GlaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,300,1050AC30.4% 67.9 / 4.0 53L184R (CTC→CGC) glaRHTH‑type transcriptional repressor GlaR
Reads supporting (aligned to +/- strand):  ref base A (26/9);  new base C (2/15);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 2.45e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTGCATGATCTGCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCAGTAT  >  ACB122/3299982‑3300234
                                                                                                                            |                                                                                                                                 
gCGTGCATGATCTGCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGagcgccagcgcc                                                                                                                        >  4:263685/1‑135 (MQ=255)
               gcAATGAGCGGTCCCGGGGTCATCCGGGGCGAGCGCATCTTGGCGCGGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGGGCCGCATGCTCGTGGCGCTTGAGCGCCAGCGCCTCTTCGGaaa                                                                                                              <  3:190867/130‑1 (MQ=255)
                 aaTGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGt                                                                                                       >  5:125409/1‑135 (MQ=255)
                           aCCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCATCATGCTCCTGGCGCTTGAGCGCCTGCGCCTCTTAGGAATACACCGTCTGCGTCAg                                                                                              >  6:421330/1‑134 (MQ=255)
                                tgtCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAg                                                                                        >  1:271552/1‑135 (MQ=255)
                                  tcatcaGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCCGCGCCGCTTCGGAAAACAACGTCTGCGTCACCCACAggg                                                                                      >  3:241235/1‑134 (MQ=255)
                                  tcatcaGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCCCAGGt                                                                                      >  4:370934/1‑135 (MQ=255)
                                  tcatcaGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGt                                                                                      >  2:243073/1‑135 (MQ=255)
                                     tccggTGCGAGCGCATCATGGCGCGGGCGGTTTTGGCAT‑CCCGGGCGAGGATCCCCGCGACCAGGGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTgg                                                                                    <  6:387634/131‑1 (MQ=255)
                                     gccggggcgcgcgcACCAGGGCGCGGGGGGTTTTGGCAG‑CCCGGGCGAGGAGCACCTCGACCAGGGCCGCTGGCTCCTGCCGCTTGCGCCCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGc                                                                                   <  6:424522/129‑1 (MQ=255)
                                       tggcgCGAGAGAATCATGGCGGGGGGGGTTTGGTCTT‑CCGGGGGGAGGTACCCATCTACCTGTGCCGAATGCGCCTGCCGGTTTCGCCCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGc                                                                                   <  8:106175/129‑1 (MQ=255)
                                       aGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGCGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCg                                                                                  >  3:299510/1‑134 (MQ=255)
                                       aGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACCCCGTCTGCGTCAGCCACAGGTGGCg                                                                                  >  5:113335/1‑134 (MQ=255)
                                       aGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCgg                                                                                 >  6:36199/1‑135 (MQ=255)
                                       aGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCgg                                                                                 >  3:91256/1‑135 (MQ=255)
                                       aGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCgg                                                                                 >  3:420543/1‑135 (MQ=255)
                                       aGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCg                                                                                  >  8:228906/1‑134 (MQ=255)
                                       aGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCg                                                                                  >  3:349149/1‑134 (MQ=255)
                                       aGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCGGGCGCTTGGGCGCCCTCGCCTCGTCGGCAAACACCGTCAGCCTGAGCCACACGTGgggg                                                                                 >  5:282536/1‑135 (MQ=255)
                                         gTGCGCGCGCATCTGGGGGGGGGGGGTTTGGGCCC‑CCGGGGCTAGTAACCCCTCGACCCGTGCCGCACGCCCCTGGCGGTCGCCCCCCCGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTa                                                                               <  4:427060/135‑1 (MQ=255)
                                                 gcATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCgg                                                                       >  3:27759/1‑135 (MQ=255)
                                                    tgttGGCGGGGGGGGTTTGGGCAT‑CCGGGGCGTGGAACACGTCGACCTGGGCCGCATGCTCCGGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGc                                                                      <  4:128431/130‑1 (MQ=255)
                                                         gcgcGGGCGGTTTGGCCAG‑CCCGGGCGAGGATCACCCCGACCTGGGCCGCCTGCCCCGGCCGCTTCACCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCg                                                               <  2:282316/135‑1 (MQ=255)
                                                            cTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGa                                                              >  7:142039/1‑133 (MQ=255)
                                                                cGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACag                                                          >  6:172281/1‑133 (MQ=255)
                                                                cGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACag                                                          >  7:306819/1‑133 (MQ=255)
                                                                  tttttGTCAG‑CGCGGGCGAGTAACACGTCCACCTGGGCCGCTGGCGCCGGGCGGTTGCGCCCCCGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACaggtag                                                      <  4:356/134‑1 (MQ=255)
                                                                  gTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGCGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACaggtag                                                      >  3:312146/1‑135 (MQ=255)
                                                                  gTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACaggtag                                                      >  1:245992/1‑135 (MQ=255)
                                                                  gTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACaggtag                                                      >  5:75498/1‑135 (MQ=255)
                                                                   ttttGGCCTGCCGGGGCGCGGAGCCCCGCGACCAGGGCCGATGGCTCGGGGCGATTGCGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACaggtag                                                      <  4:276051/135‑1 (MQ=255)
                                                                   tttgGGCAT‑CCGGGGCGAGGATCACCTCGACCAGGGCCGCTTGCTCCTGGCGCTTGAGCCCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACaggtagg                                                     <  2:103564/135‑1 (MQ=255)
                                                                        gCAG‑CCCGGCGGAGCATCACCTCGACCTGTGCCGCAGGCCCCGGGCGCTTGCGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGgtgcgt                                                <  6:75498/135‑1 (MQ=255)
                                                                                gggCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGCACAGGTAGGTGCGTGCCTgca                                         >  6:176775/1‑135 (MQ=255)
                                                                                 ggCGAGGATCACCTCGACCTGGGCCGCATGCTCCGGGCGCTTGCGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTgcag                                        <  7:343365/135‑1 (MQ=255)
                                                                                        aTCACCTCGACCTGTGCCGCATGCGCCTGGCGCTTCCGCCCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGc                                 <  7:85601/135‑1 (MQ=255)
                                                                                              tccaCCAGGGCCGCTTGCGCCTGGCCGTTGAGCGCACGCGCCTCTTCGGACAACACCGTCTGCGTCAGCGACAGGTGGCGTTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAg                           <  7:407067/132‑1 (MQ=255)
                                                                                               cGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCGCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGc                          >  1:4154/1‑135 (MQ=255)
                                                                                                    aGTGCCGCTGGCTCCTGGCGCGGGCGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAg                     <  4:299510/135‑1 (MQ=255)
                                                                                                        ccGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGcc                   >  8:157474/1‑133 (MQ=255)
                                                                                                        ccGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGcc                   >  2:310516/1‑133 (MQ=255)
                                                                                                         cGCATGCTCCTGGCGCTTTAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGa                >  7:237488/1‑135 (MQ=255)
                                                                                                         cGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGa                >  8:167976/1‑135 (MQ=255)
                                                                                                           caggCTCCTGGCGCTGGCGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAgg              <  3:376423/132‑1 (MQ=255)
                                                                                                             tGCGCCTGGCGGTTGCGCGCCCGCGCCTCTTCGGAAAACACCGTCTGCGGCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCa            <  3:112944/135‑1 (MQ=255)
                                                                                                                tcgtGGCGCGTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATg         <  4:423183/132‑1 (MQ=255)
                                                                                                                tcatGGGGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCaa           <  6:459189/130‑1 (MQ=255)
                                                                                                                tCCGGGCGGTTGCGCGCCCGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCaa           <  3:261961/133‑1 (MQ=255)
                                                                                                                gccTGGCGCGGGCGCGCCAGCGCCTCTTCGGAACACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATg         <  4:285615/134‑1 (MQ=255)
                                                                                                                cgcgcgcgcTGGCGCCCCCGCGCCTCTTCGGAAAACACCGGCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCaa           <  1:202772/128‑1 (MQ=255)
                                                                                                                  cTGGCGGTTGCGCGCCAGCGACTCTTCGGAAAACACCGGCTGCGTCAGCCACAGGGTGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGc       <  4:57282/135‑1 (MQ=255)
                                                                                                                   tGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCa      >  7:14381/1‑135 (MQ=255)
                                                                                                                   tGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCa      >  4:115328/1‑135 (MQ=255)
                                                                                                                       gcTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCAGTAt  <  2:245992/135‑1 (MQ=255)
                                                                                                                            |                                                                                                                                 
GCGTGCATGATCTGCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCAT‑CCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCAGTAT  >  ACB122/3299982‑3300234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑