Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,338,755 A→T 6.7% Y268N (TAT→AAT)  rssB_4 ← Regulator of RpoS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,338,7550AT 6.7% 249.8 / 4.8 82Y268N (TAT→AAT) rssB_4Regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base A (34/42);  new base T (3/3);  total (37/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

TTGAGCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGT  >  ACB122/3338631‑3338886
                                                                                                                            |                                                                                                                                    
ttGAGCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAgcggtcgcgg                                                                                                                            >  8:319771/1‑135 (MQ=255)
ttGAGCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAgcggtcgcgg                                                                                                                            <  8:173085/135‑1 (MQ=255)
ttGAGCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAgcggtcgcgg                                                                                                                            >  7:362437/1‑135 (MQ=255)
ttGAGCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAgcggtcgcgg                                                                                                                            >  8:451946/1‑135 (MQ=255)
ttGAGCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAgcggtcgcgg                                                                                                                            >  8:447323/1‑135 (MQ=255)
ttGAGCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAgcggtcgcgg                                                                                                                            >  3:327557/1‑135 (MQ=255)
ttGAGCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAgcggtcgcgg                                                                                                                            >  2:1734/1‑135 (MQ=255)
ttGAGCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAgcggtcgcgg                                                                                                                            >  3:251043/1‑135 (MQ=255)
   aGCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAgcggtcgcggt                                                                                                                           <  1:25115/133‑1 (MQ=255)
    gCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATTgcggtcgcgggggc                                                                                                                        >  1:219619/1‑131 (MQ=255)
    gCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGGGGGTTGTCGCGCTAGCggtcgcggtcgc                                                                                                                        >  8:204743/1‑135 (MQ=255)
     ccGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCt                                                                                                                       >  8:197276/1‑135 (MQ=255)
     ccGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCt                                                                                                                       <  5:396995/135‑1 (MQ=255)
     ccGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGGCGCt                                                                                                                       >  6:285458/1‑135 (MQ=255)
     ccGGTCGGGGAAACCGCTGCCGTCCCAGTCCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTGTGATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGCTCGCGGTCGCt                                                                                                                       >  1:283445/1‑135 (MQ=255)
           ggggAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGGCGCTGTGCa                                                                                                                  >  2:99165/1‑134 (MQ=255)
           ggggAAACCGCTGCCGTCCCAGTGCTACTGGTGATGCAGTATCAGGCGCGCTGCATCTTTCATGGGCTCAAGGGTCATCAGCATCGCGTCACTTTGGCGTGGGTAGTAGAGATAGCGGTCGCGGTCGCTGTGCa                                                                                                                  >  4:412750/1‑134 (MQ=255)
            gggAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGGCGCGATAGCGGTCGCGGTCGCTGTGCATc                                                                                                                <  3:239581/135‑1 (MQ=255)
                aaCCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACt                                                                                                            >  4:235353/1‑135 (MQ=255)
                    gCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTc                                                                                                        <  4:251043/135‑1 (MQ=255)
                         cGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGa                                                                                                     <  5:406057/133‑1 (MQ=255)
                          gTCCCTGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAATGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGGCGCTGTGCATCAACTGGTCCGAAgg                                                                                                  <  5:106306/135‑1 (MQ=255)
                          gTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAgg                                                                                                  <  7:12157/135‑1 (MQ=255)
                          gTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAgg                                                                                                  <  5:247631/135‑1 (MQ=255)
                            ccgaGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAgggg                                                                                                <  6:30051/132‑1 (MQ=255)
                            cccAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAgggg                                                                                                <  4:439444/135‑1 (MQ=255)
                            cccAGTGCGCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCGTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAgggg                                                                                                <  2:293218/135‑1 (MQ=255)
                                gTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAAc                                                                                            >  7:367218/1‑135 (MQ=255)
                                               cAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCGGTGCATCAACTGGTCCGAAGGGGCAACGAACATGCGATCCgg                                                                             >  1:184519/1‑135 (MQ=255)
                                                aGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGc                                                                            <  5:273973/135‑1 (MQ=255)
                                                  gATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCa                                                                          <  8:318229/135‑1 (MQ=255)
                                                       ggCGCGCGGCAGCTTTCATGGGCTCAAGGGTCATCAGCATCGCTTCACTTTGGAGTGGGTAGTCGCGATAGCGGTCGCTGTCTCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTc                                                                      <  2:360434/134‑1 (MQ=255)
                                                         cgcgcgGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCCACTGGTCCGCAGGGGCAACGATCCAGCTATCCGGCCAACTCAA‑t                                                                  >  6:273973/1‑135 (MQ=255)
                                                          gcgcgGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑tt                                                                 <  2:176273/135‑1 (MQ=255)
                                                            gcgGCATCTTTCATGGGCTCAAGTGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTg                                                               <  3:412750/135‑1 (MQ=255)
                                                            gcgGCATCTTTCATGGGCTCAAGTGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTg                                                               <  7:319771/135‑1 (MQ=255)
                                                            gcgGCATCTTTCATGGGCTCAAGGGTCATCAGTAGCGCTTCACTTTGGCGGGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTg                                                               <  6:87340/135‑1 (MQ=255)
                                                            gcgGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTg                                                               <  7:451946/135‑1 (MQ=255)
                                                            gcgGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTg                                                               <  7:282670/135‑1 (MQ=255)
                                                            gcgGCATCTTTCAAGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGTGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTg                                                               <  8:219262/135‑1 (MQ=255)
                                                               gCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCCTGCTATCCGGCCAACTCAA‑TTTGCCt                                                            >  8:344011/1‑135 (MQ=255)
                                                               gCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCt                                                            >  7:370882/1‑135 (MQ=255)
                                                               gCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCt                                                            <  3:235353/135‑1 (MQ=255)
                                                               gCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCt                                                            <  8:370882/135‑1 (MQ=255)
                                                                     ttCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTAGCGGTGCAGCCACTGGGCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTAtgttg                                                      >  1:19012/1‑135 (MQ=255)
                                                                            gCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGGGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAgcg                                               <  6:241027/135‑1 (MQ=255)
                                                                               cAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGGATCAACTTGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTTCAGcgccg                                            >  7:453677/1‑135 (MQ=255)
                                                                                aaGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGGATCAAAGGGTCCGCAGGGGGCACGATCCAGGACTCCCGGCCACGCAATTTTGCCT‑TGTTGTTAAGcgccgc                                           >  6:293310/1‑135 (MQ=255)
                                                                                aaGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCCTCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCCACTCAA‑TTTGCCTATTTTGTAAAGcgcc                                             >  1:269739/1‑133 (MQ=255)
                                                                                   ggTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTTCCTATGTTGTACAGCGCCGCAg                                         >  1:40738/1‑134 (MQ=255)
                                                                                     tcatcaGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAgcggtcgcggt                                                                                                                           <  5:426731/51‑1 (MQ=255)
                                                                                     tcatcaGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAgcggtcgcggt                                                                                                                           >  6:426731/1‑51 (MQ=255)
                                                                                      catcaGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGTGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCAt                                     <  5:159011/135‑1 (MQ=255)
                                                                                          agcagcGCTTCACTTTGGCGTGGGTAGTCGCGATTGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAgg                                                                                                  <  6:90982/71‑1 (MQ=255)
                                                                                          agcagcGCTTCACTTTGGCGTGGGTAGTCGCGATTGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAgg                                                                                                  <  6:97816/71‑1 (MQ=255)
                                                                                          agcagcGCTTCACTTTGGCGTGGGTAGTCGCGATTGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAgg                                                                                                  >  5:97816/1‑71 (MQ=255)
                                                                                          agcagcGCTTCACTTTGGCGTGGGTAGTCGCGATTGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAgg                                                                                                  >  5:90982/1‑71 (MQ=255)
                                                                                          agcagcGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCTAACGGGCAAAGATAATTCTATCCGGCCAACTCCA‑TTTTCCTATGTTGTACACAGCCGCAGCCAAGGcc                                 >  8:78861/1‑135 (MQ=255)
                                                                                          agcagcGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATACGGCCAACTCAA‑TTTTCCTATGTTGTACAGCGCCGCAGCCATggac                                 >  1:420496/1‑133 (MQ=255)
                                                                                               cgcTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATc                            >  8:132044/1‑135 (MQ=255)
                                                                                                  ttCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATcgcg                         >  2:47275/1‑135 (MQ=255)
                                                                                                  ttCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATAATGCTATCCGGCCAACTAAA‑TTTGCCAATGTTGTACAGCGCCCCAGCCATGGCCAGATcgcg                         >  5:237551/1‑135 (MQ=255)
                                                                                                    cACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGc                       >  3:222743/1‑135 (MQ=255)
                                                                                                          ggCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGAt                   <  5:41060/133‑1 (MQ=255)
                                                                                                          ggCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGAt                   <  1:86342/133‑1 (MQ=255)
                                                                                                          ggCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATc                  <  4:213753/134‑1 (MQ=255)
                                                                                                           gCGGGGGTAGACGCGAGAGCGGTCGCGGTCGCTGTGCATCAACTGGGCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATc                  <  4:131310/133‑1 (MQ=255)
                                                                                                            cGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAgg                                                                                                  >  7:299430/1‑53 (MQ=255)
                                                                                                            cGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAgg                                                                                                  <  8:299430/53‑1 (MQ=255)
                                                                                                            cGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTTCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGcc               >  4:377453/1‑135 (MQ=255)
                                                                                                               gggTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCg            <  2:9479/135‑1 (MQ=255)
                                                                                                               gggTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTCATCGCCTCg            >  8:289128/1‑135 (MQ=255)
                                                                                                                ggTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGt           <  7:28756/135‑1 (MQ=255)
                                                                                                                     tCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCaa       <  7:447323/134‑1 (MQ=255)
                                                                                                                       gcgATTGCGGGCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGc    <  2:219619/135‑1 (MQ=255)
                                                                                                                       gcgATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGc    <  6:23074/135‑1 (MQ=255)
                                                                                                                       gcgATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGc    <  8:301461/135‑1 (MQ=255)
                                                                                                                       gcgATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGc    <  3:377453/135‑1 (MQ=255)
                                                                                                                       gcgATAGCGGTCGCGGTCGCTGTGCATCAACTGGGCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGc    <  7:78861/135‑1 (MQ=255)
                                                                                                                         gATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGt  <  1:99165/135‑1 (MQ=255)
                                                                                                                         gATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGt  <  7:132044/135‑1 (MQ=255)
                                                                                                                         gATAGCGGTCGAGGTCGCTGTGCATCAACTGGGCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGACAGATCGCGGCTGATCGCCTCGTCCAAGGctt  <  4:62809/135‑3 (MQ=255)
                                                                                                                            |                                                                                                                                    
TTGAGCCGGTCGGGGAAACCGCTGCCGTCCCAGTGCTCCTGGTGATGCAGGATCAGGCGCGCGGCATCTTTCATGGGCTCAAGGGTCATCAGCAGCGCTTCACTTTGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAA‑TTTGCCTATGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGT  >  ACB122/3338631‑3338886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑