Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,389,709 T→G 32.4% L121R (CTG→CGG)  yfdE → Acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,389,7090TG32.4% 77.1 / 5.4 59L121R (CTG→CGG) yfdEAcetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base T (5/33);  new base G (16/3);  total (23/36)
Fisher's exact test for biased strand distribution p-value = 2.34e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGGACCCGCGCGGCAAGGCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGATCATGCAGATGGTGGGCGTGCCGGGCGAGGCACCGCCGCT  >  ACB122/3389575‑3389838
                                                                                                                                      |                                                                                                                                  
gAAGGACCCGCGCGGCAAGGCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCt                                                                                                                                    >  8:201796/1‑135 (MQ=255)
    gccccGCGCGGCAAGGCGTTCATGCAGCGCATGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCCGCGCGCCACCCCCCCCTgggg                                                                                                                                >  8:182431/3‑133 (MQ=255)
        cgcgcgGCAAGGCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACt                                                                                                                            <  2:87952/135‑1 (MQ=255)
          cgcgGCAAGGCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCGGGGGTACGGc                                                                                                                          >  5:196891/1‑135 (MQ=255)
           gcgGCAAGGCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCCACCCGCGCCGGGGGTTCGGCt                                                                                                                         >  7:327274/1‑135 (MQ=255)
             ggCAAGGCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCT‑GGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGa                                                                                                                     <  2:267668/135‑1 (MQ=255)
             ggCAAGGCGTACATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCCCCCCGGGGGTACTGCT‑c                                                                                                                       >  3:353565/1‑134 (MQ=255)
              gCAAGGCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGa                                                                                                                     <  8:437130/135‑1 (MQ=255)
              gCAAGGCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGa                                                                                                                     <  8:356365/135‑1 (MQ=255)
                 aGGCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCCGCCCGCCCCCCGCCCCCGGTGTACTGCT‑AGAgtt                                                                                                                  >  6:365833/1‑135 (MQ=255)
                   gCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTcc                                                                                                                <  5:191793/135‑1 (MQ=255)
                   gCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTcc                                                                                                                <  6:249567/135‑1 (MQ=255)
                   gCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTcc                                                                                                                <  3:428285/135‑1 (MQ=255)
                   gCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTcc                                                                                                                <  2:64824/135‑1 (MQ=255)
                   gCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCGGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTcc                                                                                                                <  6:201079/135‑1 (MQ=255)
                   gCGTTCATGCAGCGCCTGACCGATTCGCGCGACGTGTTCAGCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTcc                                                                                                                <  1:223938/135‑1 (MQ=255)
                   gCGTTCAGGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTcc                                                                                                                <  7:244421/135‑1 (MQ=255)
                          tGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCCCAACCCGCGCCGGGGATACAGCT‑CGATTTCCCCCAACg                                                                                                         >  1:66347/1‑135 (MQ=255)
                                 ccTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCCACCCGCGCCGGGTGTACTGCT‑CGATTTCCGCCTACGGCCAAAc                                                                                                  >  1:306684/1‑135 (MQ=255)
                                 ccTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATAc                                                                                                  <  8:118182/135‑1 (MQ=255)
                                   tGACCGATTCGGCCGACGGGTTCAGCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCGGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCg                                                                                                <  7:27392/135‑1 (MQ=255)
                                    gACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCCACCCGCGCCGGGTGTACTGCT‑CGCTTTCCGCCAACGGCCCTAcccg                                                                                               >  3:406258/1‑133 (MQ=255)
                                            cGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCCACCCCCGCCGGGGGGACTGCG‑CGCTTTTCGCCATCCGCCCTACCGGGcccggcc                                                                                       >  4:283016/1‑130 (MQ=255)
                                               ccGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCACCCCGCGCCGGGGGTACTGGG‑CGATTTCCGCCTACGGCCATACCGGGCCGGCCGccc                                                                                    >  6:226394/1‑135 (MQ=255)
                                                  aCGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGGCACCCCCGCCGGGGGTACGGCT‑CGATTTCCGCCTACGGCCCTACCGGGCCGGCCGcccccc                                                                                 >  4:36356/1‑135 (MQ=255)
                                                      gtTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGCTTTCCGCCTACGGCCATACCGGGCCGGACGCCCCCCgcgc                                                                             >  2:271237/1‑135 (MQ=255)
                                                            cGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCCCCACCCGCCCCCGGTGTAAGGCT‑CGCTTTACGCCTAACGCCATACCCCGCCCGACGCCCCCCGCCCCCGCtt                                                                       >  6:316053/1‑135 (MQ=255)
                                                                        ccccGGCGCCCTGGACACGCTGGGCCTGGGCTACGCCGAGCTGGCGGCGCGCAACCCGGGGCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCGCACCGCGCCGGCTTCGGTCTGATCGc                                                           <  1:51465/135‑1 (MQ=255)
                                                                           cGGCGCCCTGGACAAGCTGGGCCTGGGCTACGACGAGCTGTCGGCCCGCAACCCGCGCCGGGTGGACTGAG‑CGATTTACGACAACGGCCATAACGCGCAGGCCGCCCCCCCCCCCGCCGTCGCTATGATAGCCGa                                                        >  4:379725/1‑135 (MQ=255)
                                                                            ggCGCCCTGGACACGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAg                                                       <  5:255995/135‑1 (MQ=255)
                                                                            ggCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCGTCGGTCTGATCGCCGAg                                                       <  8:134936/135‑1 (MQ=255)
                                                                            ggCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGGCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAg                                                       <  8:294823/135‑1 (MQ=255)
                                                                                cccTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCGGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCGTCGGTCTGATCGCCGAGGCCa                                                   >  4:382577/1‑135 (MQ=255)
                                                                                 ccTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCaa                                                  <  1:394707/135‑1 (MQ=255)
                                                                                      aCAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCCACCCGCGCCGGGGGTTCTGCG‑CGCTTTACCCCTACGGCCCTACCCGGCCGGCCGCCCCCCCCGCCGGCGTCGGTCTGGTCGCCCCGGCCCAGAGCg                                             >  3:344531/1‑135 (MQ=255)
                                                                                      aCAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCg                                             <  4:394853/135‑1 (MQ=255)
                                                                                      aCAAGCTGGGCCTGGGCTACGCCGAGCTGGCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAagag                                               <  7:42876/133‑1 (MQ=255)
                                                                                      aCAAGCTGGGCCTGGGCGACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCg                                             <  4:163457/135‑1 (MQ=255)
                                                                                       cAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCATCCCGCGCGTGGTGTTCTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCTAGAGCgg                                            <  2:132417/135‑1 (MQ=255)
                                                                                       cAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCgg                                            <  8:44797/135‑1 (MQ=255)
                                                                                       cAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCgg                                            <  7:194466/135‑1 (MQ=255)
                                                                                       cAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGCCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCgg                                            <  1:170635/135‑1 (MQ=255)
                                                                                       cAAGCTAGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTTTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCgg                                            <  6:419217/135‑1 (MQ=255)
                                                                                           ctgggcctgggcTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGATc                                        <  3:382577/135‑1 (MQ=255)
                                                                                           ctgggcctgggcTACGCCGAGCTGGCGGCGCGCAACCCGCGCCGGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGATc                                        <  2:12906/135‑1 (MQ=255)
                                                                                               gcctgggcTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGATCAt                                      <  7:230550/133‑1 (MQ=255)
                                                                                                  tgggcTACGCCGATCGGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGAGCATGCAGa                                 <  7:182431/135‑1 (MQ=255)
                                                                                                       tACGCCGAGCTGTCGGCGCGCCACCCGCGCCGGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCCCCGCGCCGGCTTCGGTCGGATCGCCGCGGCCAAGAGCGGGATCATGCCGAtggtg                            >  1:403255/1‑135 (MQ=255)
                                                                                                       tACGCCGAGCTGTCGGCGCGCCACCCCCGCCGGGGGTTCTGGG‑CGATTTGCGCCAACGGCCCTACCGGGCCGGCCGCCCCCCCCGCCCGCGTCGGTGTGATAGCCGCGGCCAAGCGCCGGGTCATGCAGAtggtg                            >  6:32467/1‑135 (MQ=255)
                                                                                                           ccGAGCTGTGGGCGCGCAAACCCAGGCTGGTTTACTGCTGCGA‑TTACGCCGAAGGGCAATCCCGGCCCGAAGCCCACCCCCCCCGCGTCAGTAGTAACGCCGAGGCCAAGAGCGGGCTCATGTAGCTGGTggggc                        >  6:442950/1‑133 (MQ=255)
                                                                                                            cGAGCTGGCGGCGCGCAACCCGCGCCGGGTGTACTGCT‑CGATTGCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTGCGGTCTGATCGCCGAGGCCAAGAGCGGGATCATGCAGATGGTGGGCGt                       <  1:80963/135‑1 (MQ=255)
                                                                                                               gCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGCCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGATCATGCAGATGGTGGGCGTGcc                    <  4:411063/135‑1 (MQ=255)
                                                                                                                 tGTCGGCGCGCCACCCGCGCCGGGTGTTCGGCG‑CGCTTTCCGCCTACGGCCCTACCGGGCCGGACGCCCCCCGCGCCCGCGTGGGTCCGCTAGCCGAGGGCCAGAGCGGGATCATGCAGATGGTGGGCGTGCCgg                  >  2:3597/1‑135 (MQ=255)
                                                                                                                 tGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGATCATGCAGATGGTGGGCGTGCCgg                  >  3:351685/1‑135 (MQ=255)
                                                                                                                 tGTCGGCGCGCAACCCGCGCCTGGTGTACTGCG‑CGATTTCCGCCTACGGCCATACCCGGCCCGCCGCCCCCCCCGCCGGCGTCGGTCTGCTAGCCGCGGCCAAGCGCGGGATGATGCAGATTGTGGGCGTGCCgg                  >  8:432212/1‑135 (MQ=255)
                                                                                                                      gcgcgcAACCCGCGCCGGGTGTACTGCG‑CGATTTCCGCCTACGGCCATACCCGGCCCGACGCCCCCCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGATCATGCAGATGGTGGGCGTGCCGGGCGAg             >  4:337018/1‑135 (MQ=255)
                                                                                                                       cgcgcAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGATCATGCAGATGGTGGGCGTGCCGGGCGAgg            <  6:196891/135‑1 (MQ=255)
                                                                                                                            aaCCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGATCATGCAGATGGTGGGCGTGCCGGGCGAGGCAccg       <  3:346252/135‑1 (MQ=255)
                                                                                                                               ccGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGATCATGCAGATGGTGGGCGTGCCGGGCGAGGCAccgccg    <  1:251374/135‑1 (MQ=255)
                                                                                                                                 gcgcCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGATCCTGCAGATGGTGGGCGTGCCGGGCGAGGCACCGCCgct  <  2:170458/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
GAAGGACCCGCGCGGCAAGGCGTTCATGCAGCGCCTGACCGATTCGGCCGACGTGTTCATCGAGAACTACCGCCCCGGCGCCCTGGACAAGCTGGGCCTGGGCTACGCCGAGCTGTCGGCGCGCAACCCGCGCCTGGTGTACTGCT‑CGATTTCCGCCTACGGCCATACCGGGCCGGACGCCCACCGCGCCGGCTTCGGTCTGATCGCCGAGGCCAAGAGCGGGATCATGCAGATGGTGGGCGTGCCGGGCGAGGCACCGCCGCT  >  ACB122/3389575‑3389838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑