| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,432,092 | C→G | 17.8% | intergenic (+51/‑44) | CJ019_03257 → / → gpFI | hypothetical protein/Putative prophage major tail sheath protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,432,092 | 0 | C | G | 17.8% | 105.6 / 2.6 | 52 | intergenic (+51/‑44) | CJ019_03257/gpFI | hypothetical protein/Putative prophage major tail sheath protein |
| Reads supporting (aligned to +/- strand): ref base C (10/31); new base G (7/2); total (19/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.43e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTCATGACGAGGCCGAAGCTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCT > ACB122/3431961‑3432208 | aCTCATGACGAGGCCGAAGCTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCACCCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑GTGc > 1:7550/1‑135 (MQ=255) cGAAGCTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCcgcg > 7:71527/1‑135 (MQ=255) gAAGCTATTGCCGACGAGGCCATTGCCGTGATCAACGCACGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTGT‑TCGGTT‑CGGCGGGGCCGCCCcccgc > 8:95542/1‑135 (MQ=255) gAAGCTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCACCCCGCCTTCCTTGAGCGCCCCGTCCAGCGGGGCGGGG‑TGGGTT‑GTGGGGGGGCGCCCcccgc > 7:414694/1‑135 (MQ=255) gAAGCTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTTGTAGTTT‑TGGCGGGGCccccccccc > 1:180577/1‑132 (MQ=255) gAAGCTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTGT‑TGGGTT‑GGGCGGGGGCGCCCcccgc > 5:364443/1‑135 (MQ=255) gAAGCTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGGGT‑TGGGTT‑GTGGGGGGCCCCCCcccgc > 5:464180/1‑135 (MQ=255) gAAGCTATTGACGACGAGGCAATTGACGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCCACCCGCCTTCCTTGAGCGCCCCGTCAATCGGGGCGTTT‑TGCGTT‑CTGCGGGGCCGCCCcccgc > 8:314061/1‑135 (MQ=255) aaGCTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCcgcgcg < 3:151900/135‑1 (MQ=255) aGCTATTGCCGACGAGGCCATTGCCGTGATCCACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGACCGCCCCGGCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCgg < 7:218178/135‑1 (MQ=255) aGCTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTGT‑TCGGTT‑GTGCGGGGCCGCCCCGCGCgg > 3:280647/1‑135 (MQ=255) aGCTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCAGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCTTTT‑CTGCGGGGCCGCCCCGCGCgg < 8:101485/135‑1 (MQ=255) gCTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCGTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCgg < 1:317365/134‑1 (MQ=255) cTATTGCCGCCGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGcc < 4:162116/135‑1 (MQ=255) cTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TGGGTT‑GTGGGGGGCCGGCCCGCGCGGcc > 1:233308/1‑135 (MQ=255) cTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGAAACGCCTGACCCGCAACCCGCCTTCCTTGCGCGCCCCGTCCATCGGGGGGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGcc < 8:25910/135‑1 (MQ=255) cTATTCCCGACGAGGCCATTGCCGTGATCACCGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAAGCCGCCTTCCTTGAGCGCCCCGGCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGcc < 8:453139/135‑1 (MQ=255) tttGCCGACGAGGCCATTGCCGTGATCTACGCATGCGGGGTCGGTGTCGGCAACGCCTGACCGGCAACCCGGGTTCCTTGAGCGCCCCGTGCTGCGGGGCGTTT‑TCGTTT‑GTGCGGGGCGGCCCCGCGCGGCCCt < 7:38478/134‑1 (MQ=255) tGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCt < 7:93862/133‑1 (MQ=255) tGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCGTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGACCGCCCCGCGCGGCCCt < 6:451388/133‑1 (MQ=255) ccGACGAGGCCATTGCCGTGATCAACGCAAGCGTGTGCGGTGTCGGCAACGCCTGACCCGCAACCAGCCTTCCTTGAGCGCCCCGTCCATCGGGGAGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGt < 6:74705/134‑1 (MQ=255) ccGACGAGGCCATTGCCGTGATAAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGCCCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCAGCGGGGCGTTT‑TGGGTT‑AGTGGGGGGCGCCCCCCGCGGgccggggt > 3:282230/1‑130 (MQ=255) aGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTTCGGGGCCGCCCCGCGCGGCCCGTGTCTTAtgt > 8:135143/1‑133 (MQ=255) aGGCCATTGCCGTGATCAACGCAAGCGGGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCt < 5:233489/135‑1 (MQ=255) ccATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGa < 6:318679/135‑1 (MQ=255) tCAACGCATGCGTGGTCGGGGGCGGAAACGCCGGACCCGCAACCCGCCTTCCTTGAGCGCCCCTTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTGGTCTTATCTGGAGCCCACATGACt < 6:149536/135‑1 (MQ=255) tCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTGT‑TGGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCGTGTCTTTTCTGGGGCCCACATGAcg > 8:406512/1‑134 (MQ=255) aCGCAAGCGGGGTGGGTGTGGGAAACGGGGTACCCGCAACCCGCCTTCCTTCCGCGCCCCGTCCTGCGGGG‑GTTT‑TTGTTTGCTGCGGGGCCGCCCGGCGCGGCCGTTGTCTTATCTGGAGCCCACATGACTgg < 8:170168/134‑1 (MQ=255) aaGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGa < 8:63171/116‑1 (MQ=255) aaGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCGTGTCTTATCTGGa > 7:63171/1‑116 (MQ=255) aaGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTGT‑TGGTTT‑CTGCGGGGCCGCCCCGCGCCGCCCGGGTCGTATGTGGAGGCCACAATACGGGGTTCt > 7:202145/1‑135 (MQ=255) cgCGTGGTGGGTGTCGGCAAGGCCTGACCCGAAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCtt < 8:48915/134‑1 (MQ=255) aGCGTGGGCGGTGGCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCtt < 7:441855/135‑1 (MQ=255) gggcGGGGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGCGCGCCCCGGCCATCGGGGCGTTT‑TCGTTG‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACg < 1:293745/132‑1 (MQ=255) ggTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTGGGCTTATCTGGGGCCCCCATGTCTGGATTCTTTCACGGCGt > 4:314924/1‑135 (MQ=255) cGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TGGGTT‑CTGCGGGGCCGCCCCGCGCGGCCCGTGTCGGATCGGGGGGCCCCATGGCGGGGTTGTTTGACGGCGGTGCCg > 5:175491/1‑135 (MQ=255) aCGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑GTGCGGGGCCGCCCCGCGCGGGCCTTGTGTTTTATGGCGCCCACATGACTGGATTGTTTAACGGCGTGACCGGaac > 4:226030/1‑134 (MQ=255) gCCTGACCCGAAACCCGCCTTCCTTGAGCGCCCGGTCCTTGGGGGCGTTT‑GCTTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTaacgaa < 6:464180/135‑1 (MQ=255) cccGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TGGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGa > 7:243418/1‑91 (MQ=255) cccGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGa < 8:243418/91‑1 (MQ=255) ccGCCTTCCTTGCGCGCCCCGGCCATCGGGGCGTTT‑TCGTTT‑CGGCGGGGCCGCCCCGCGCGGCCCTGGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCgggg < 2:414960/135‑1 (MQ=255) cGCCTTCCTTGAGCGCCCCGTCCATCGGGGGGTTT‑TCGGTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGgc < 8:452324/135‑1 (MQ=255) cGCCTTCCTTGAGCGCCCCGGCCATCGGGGAGTTT‑TCGTTG‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGgc < 4:380028/135‑1 (MQ=255) cttGCGCGCCCGGTCCCGCGGGGCGTTT‑TCGTTG‑CGGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCgca < 2:265590/133‑1 (MQ=255) cttGAGCGCCCCGTCCCGCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCgca < 1:30835/133‑1 (MQ=255) cttGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCgca < 8:71527/133‑1 (MQ=255) ttccgcgcCCCGCCCAGGGGGGCGTTT‑GCGTTG‑CGGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCgcagca < 1:447690/131‑1 (MQ=255) cgcCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTgcg < 8:76058/135‑1 (MQ=255) cccGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCGTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTg < 6:220925/135‑1 (MQ=255) ctCGGGGCGTTT‑TCGTGT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGtcct < 2:366379/134‑1 (MQ=255) aTCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGtcct < 8:363709/135‑1 (MQ=255) aTCGCGGGGTTT‑TCGATT‑GTGCGGGGCCGCCCCGCTCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGtcct < 8:71823/135‑1 (MQ=255) | ACTCATGACGAGGCCGAAGCTATTGCCGACGAGGCCATTGCCGTGATCAACGCAAGCGTGGTCGGTGTCGGCAACGCCTGACCCGCAACCCGCCTTCCTTGAGCGCCCCGTCCATCGGGGCGTTT‑TCGTTT‑CTGCGGGGCCGCCCCGCGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCT > ACB122/3431961‑3432208 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |