| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,465,424 | T→G | 22.9% | A41A (GCA→GCC) | fcuA_1 ← | Ferrichrome receptor FcuA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,465,424 | 0 | T | G | 22.9% | 94.5 / 7.0 | 58 | A41A (GCA→GCC) | fcuA_1 | Ferrichrome receptor FcuA |
| Reads supporting (aligned to +/- strand): ref base T (9/32); new base G (12/3); total (23/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAAGCCTTGCGCGATACTGTAGCTGCCCTCCAGGCCTTGGCTTTGTACGCCCCTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGCGTCATGCGTGGGTC > ACB122/3465294‑3465555 | cAAAGCCTTGCGCGATACTGTAGCTGCCCTCCAGGCCTTGGCTTTGTACGCCGCTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCTAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCgg < 7:185937/135‑1 (MQ=255)cAAAGCCTTGCGCGATACTGTAGCTGCCCTCCAGGCCTTGGCTTTGTACGCCCCTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCgg < 4:199431/135‑1 (MQ=255) ttGCGCGATACTGTAGCTGCCCTCCAGGCCTTGGCTTTGTACGCCCCTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAggtg < 8:456201/135‑1 (MQ=255) tGTAGCTGCCCTCCAGGCCTTGGCTTTGTACGCCCCTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGGGGGCAAGCGCGCCCGTTCAT‑CGGG‑CCCAC‑GGCCGGCGGCCAGGGGGGAAGTgcggg > 1:70004/1‑133 (MQ=255) tGTAGCTGCCCTCCAGGCCTTGGCTTTGTACGCCCCTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGGGGGCAAGCGCGCCCGGTCAT‑C‑GG‑GCCACGGGCCGGCGGCCCGGGGGGTAGTgggcg > 6:181454/1‑135 (MQ=255) tGTAGCTGCCCTCCAGGCCTTGGCTTTGTACGCCCCTGAGCAGGGCCGGGGCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTgcgc < 7:193840/134‑1 (MQ=255) cccTCCAGGCCTTGGCTTTGTACGCCCCTGAGCAGGGCCGGGTCGAATGATAACGCCACCCCGGCGTT‑GGGGGCCAACCGCGCCCGTTCAT‑A‑GG‑CCCACCGGCCGGCGGCCCGGGGGGTAGTGCGCGCGGGGgtg > 2:30438/1‑135 (MQ=255) cccTCCAGGCCTTGGCTTTGTACGCCCCTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGGGGGCAACCGCGCCCGGACCT‑C‑GGCCCCCCGGGCGGGCGCCCCGGGGGT‑AGTGCGCGCGGTgggg > 8:302043/1‑133 (MQ=255) cgccAGGCCTTGTTTTTGTACGCCCCTAAGAAGGGCGGGGTCTAATTCCAACGCCACCCAGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTc < 2:405904/133‑1 (MQ=255) cTCCAGGCCTTGGCTTTGTACGCCCCTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGGGGCCAAACGCGCCACGACCT‑C‑GG‑GCAACCGGCCGGGGGGCCCGTGGTTAGTGGGGGCGGAgggggg > 4:430790/1‑131 (MQ=255) cTCCAGGCCTTGGCTTTGTACGCCCCTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGCCGCCAAGCGCGCCCCTAAAT‑A‑GG‑CCCACCGGCCTGCGGCCACGTGGGAAATGGCCGCGGTGgtgtg > 8:335143/1‑134 (MQ=255) cAGGCCTTGGCTTTGTACGCCCCTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTG‑GGCGGTAAAACGCCCCCCTACAA‑C‑GG‑GCAACCGGCCGGCGGCCAGGTGGGTAGATCGCGCGGAGGTGTCCGc > 1:449857/1‑135 (MQ=255) tttGTACGCCCCTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGGGGCAAAGCCCGCCAGTACCTAG‑GG‑CCACCGGGCGGGGGGCC‑GGGGGTTAGTGCGCGGGGGGGGGTGGGCTTCCCTCgg‑gg > 4:138904/1‑135 (MQ=255) cccTGAGCCGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCa < 8:413353/135‑1 (MQ=255) cTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTTGGGCGGCAACCGCCCCCCGAAAAA‑G‑GC‑CCACCCGGCCGGCGG‑CACGTGGGTAGTTCGCGGGGCGGTGTCGGCAGCCCACGC‑CCTggggggccgg > 4:45638/1‑130 (MQ=255) cTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGCGGGCAACCCCCCCCGTACAT‑C‑GG‑GCCCCCGGCCGGCGGCCGCGTGGTTATTGCGGCCGGGGGTGTGGGCTGCCCTCGC‑GGGGGGGGGCAgg > 2:122979/1‑135 (MQ=255) tGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGGGGCCAACCGCGCCCGTACAA‑C‑GG‑GCCACGGGGCAGGGGCCCCGGGGGTAGTGGGCGCGGGGGGGTCGGCTGGCCTAgg‑gggggggggcccgg > 1:68364/1‑129 (MQ=255) tGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGGGGCAAAAAGCGCCATTACAT‑A‑CG‑CCAAACGGCCTGCGGCCACGTGGGTACTGCGCGCCGCGGTGTCGGCAGCCAAAGC‑CGGGGGGGGCAggg > 2:296593/1‑135 (MQ=255) tGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGCGGGCAACCGCGCCCGGTAAT‑C‑GG‑GCAACGGGCCAGCGGGCCGGTGGGTAATGCGCGCGGTGGGGTGGGCTGCCCTAGC‑GGGGGGGGGCCggg > 4:94964/1‑135 (MQ=255) gctgggCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGggcg < 3:122584/131‑1 (MQ=255) gCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGggcgg < 6:391184/135‑1 (MQ=255) ggCCGGGGCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCAt < 4:280768/135‑1 (MQ=255) gCCGGGCCGAATCACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATg < 5:51698/135‑1 (MQ=255) cGGGTCGAATGACAACGCCCCCCCGGCGTT‑GGGGGCACAGCGCGCCAGGAAAT‑A‑GG‑GCCACGGGGCGGGGGGCCGGGGGGTAGTGGGCGCGGTGGTGGGGGGGGGCCTCGC‑GGGGGGGGGCCGGGCGGGCCTggg > 8:448846/1‑134 (MQ=255) cGGGTCGAATGACAACGCCACCCCGGCGTT‑GGGGGCCAACCCCGCCCGTTCAA‑C‑GG‑GGCACGGGCCGGCGGCCCGGGGGGTATTGGGCGCGGGGGGGTGGGGTGCCCTAGCGGGGGGGGGCCGGGGGGGCCAggg > 4:206229/1‑135 (MQ=255) cGGGTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCCCGACAT‑C‑GG‑CCCACCGGCCTGCGGGCCGGGGGTTAGTGGGCGGGGAGGGGTCGGGTGCCCTCGC‑GGGGGGGGGCCGGGCGGcccaggg > 6:205460/1‑130 (MQ=255) gccGAATCACAACGCCCCCCCGGCGTG‑GGCGGCAACGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGAt < 1:168668/133‑1 (MQ=255) gTCGAATGACAACGCCACCCCGGCTTT‑GGCGGCAACGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCTGGTGGTAAGTGCGCGCTGAGGTGTCGGCTGCAATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGAt < 7:56682/135‑1 (MQ=255) gTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGAt < 4:180628/135‑1 (MQ=255) gTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGAt < 4:77879/135‑1 (MQ=255) gTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGAt < 7:63462/135‑1 (MQ=255) gTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCCGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGAt < 4:252718/135‑1 (MQ=255) tCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATg < 7:137553/135‑1 (MQ=255) tCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATg < 4:85042/135‑1 (MQ=255) tCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATg < 4:389780/135‑1 (MQ=255) tCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGGACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATg < 6:43722/135‑1 (MQ=255) cGAATGACAACGCCACCCCGGCGTT‑GGGGGCCCAGCGCGGCAGTGCAA‑C‑GG‑GCCACCGGGCGGCGGGCAGGGGGGTAGTGGGGGCGGCGGTGTCGGGGGCCCTCGC‑GGGGGGGGGCAGGGCGGGCCAGGCGAggg > 6:441475/1‑135 (MQ=255) cGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTTGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAg < 3:308645/135‑1 (MQ=255) cGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAg < 3:428547/135‑1 (MQ=255) cGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAg < 4:297219/135‑1 (MQ=255) t‑GGCGGCAAAGCGCGCCTGTACAT‑C‑GG‑CCAACGGGCCGGCGGCCAGGTTGTATGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGACATGGCGATGGCTAGGCTTAGGGCCTTCatatgct < 4:182420/135‑7 (MQ=255) gcgcCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTTGTAAGTGCGCGCGGAGGTGGCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCt < 8:323066/135‑1 (MQ=255) gcgcCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCTGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTATGGCCTTCAAAGGCAAGCGGTACGGCt < 5:183364/135‑1 (MQ=255) gcCAGTCCAT‑C‑GG‑CCAACGGGCCGGCGGCCAGGTGGTAAGTGCGCGCGGAGGGGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTgg < 3:288687/135‑1 (MQ=255) gcCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGGGGTAAGTGCGCGCGGAGGTGGCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTgg < 1:304093/135‑1 (MQ=255) ccAGTACCT‑C‑GG‑CCAAGGGGCGGGGGGGCTGGTGGTACGGGCGCGCGGAGGGGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGc < 5:18338/135‑1 (MQ=255) cAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTgg > 6:146096/1‑133 (MQ=255) aCAG‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGGGGCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGc < 1:429764/135‑1 (MQ=255) aT‑C‑GT‑CCAACGGGCCTGCGGCCAGGTGGTAAGTTCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGCg < 7:192101/134‑1 (MQ=255) gg‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGCGTCAt < 8:202402/135‑1 (MQ=255) g‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGCGTCAt < 5:148347/134‑1 (MQ=255) g‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGCGTCATg < 1:304758/135‑1 (MQ=255) aaCGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGCGTCATGCGt > 5:256157/1‑135 (MQ=255) gggCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGGGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGCGTGAAGGGTggg > 1:456958/1‑135 (MQ=255) gggCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGCGTCATGCGTggg > 5:409431/1‑135 (MQ=255) ggCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGCGTCATGCGTGGGt < 5:443327/135‑1 (MQ=255) gCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGCGTCATGCGTGGGTc > 4:457224/1‑135 (MQ=255) gCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGACCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGCGTCATGCGTGGGTc > 6:154473/1‑135 (MQ=255) | CAAAGCCTTGCGCGATACTGTAGCTGCCCTCCAGGCCTTGGCTTTGTACGCCCCTGAGCAGGGCCGGGTCGAATGACAACGCCACCCCGGCGTT‑GGCGGCAAAGCGCGCCAGTACAT‑C‑GG‑CCAACGGGCCTGCGGCCAGGTGGTAAGTGCGCGCGGAGGTGTCGGCTGCCATCGC‑GGTGGGGGCCAGGGCGGCCATGGCGATGGCTAGGCTTAGGGCCTTCAAAGGCAAGCGGTACGGCTGGCGAGGCGTCATGCGTGGGTC > ACB122/3465294‑3465555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |