Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,473,321	G→T	5.5%	Q319K (CAG→AAG)	ompA_2 ←	Outer membrane protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,473,321	0	G	T	5.5%	218.9 / 4.1	73	Q319K (CAG→AAG)	ompA_2	Outer membrane protein A
Reads supporting (aligned to +/- strand):  ref base G (33/36);  new base T (2/2);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.77e-01

TGGTAAGGGTTGGCACGCAGCAGGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCATCACTTCACCACGCGCGGCGTCGTCCA  >  ACB122/3473191‑3473455                                                                                                                                   |                                                                                                                                       tGGTAAGGGTTGGCACGCAGCAGGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTgg                                                                                                                                    >  2:242533/1‑135 (MQ=255)    tAAGGGTTGGCACGCAGCAGGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTc                                                                                                                                 >  6:192503/1‑135 (MQ=255)           tGGCACGCAGCAGGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCt                                                                                                                          <  7:366892/135‑1 (MQ=255)              cACGCAGCAGGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGcc                                                                                                                       >  6:235349/1‑135 (MQ=255)                cGCAGCAGGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCt                                                                                                                          >  5:398038/1‑130 (MQ=255)                cGCAGCAGGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCt                                                                                                                          <  6:398038/130‑1 (MQ=255)                cGCAGCAGGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGccc                                                                                                                      >  8:16133/1‑134 (MQ=255)                 gcagcaGGCTGTCGACGAACTGATGGAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGccccc                                                                                                                    <  3:290246/135‑1 (MQ=255)                     cagGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTa                                                                                                                >  7:131347/1‑135 (MQ=255)                     cagGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTa                                                                                                                >  7:221252/1‑135 (MQ=255)                     cagGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTa                                                                                                                >  8:134946/1‑135 (MQ=255)                     cagGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTa                                                                                                                >  2:187008/1‑135 (MQ=255)                     cagGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTa                                                                                                                >  5:240837/1‑135 (MQ=255)                     cagGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTa                                                                                                                >  8:219068/1‑135 (MQ=255)                       ggCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAAc                                                                                                              <  5:126988/135‑1 (MQ=255)                              gacgaACTGATGCAGGCGCGGCTTGATCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAg                                                                                                       <  3:439777/135‑1 (MQ=255)                               acgaACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGc                                                                                                      >  3:306813/1‑135 (MQ=255)                               acgaACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGc                                                                                                      >  1:169266/1‑135 (MQ=255)                                cgaACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCt                                                                                                     >  2:156790/1‑135 (MQ=255)                                 gaACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTc                                                                                                    <  8:280167/135‑1 (MQ=255)                                        tGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCa                                                                                               <  7:219068/133‑1 (MQ=255)                                        tGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCa                                                                                               <  5:154209/133‑1 (MQ=255)                                        tGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCa                                                                                               <  3:432997/133‑1 (MQ=255)                                        tGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATc                                                                                             <  3:379681/135‑1 (MQ=255)                                        tGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATc                                                                                             <  8:117397/135‑1 (MQ=255)                                        tGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATc                                                                                             <  7:441989/135‑1 (MQ=255)                                              cgcgGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTg                                                                                       <  8:70772/135‑1 (MQ=255)                                              cgcgGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTg                                                                                       <  7:435069/135‑1 (MQ=255)                                                cgGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTTGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGc                                                                                      >  5:81934/1‑134 (MQ=255)                                                  gCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCCTCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCt                                                                                     >  6:32851/1‑133 (MQ=255)                                                    ttGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAg                                                                                 >  8:80171/1‑135 (MQ=255)                                                          gcgGCCAGCTCCAGCGGGAGGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTgg                                                                           >  5:130169/1‑135 (MQ=255)                                                          gcgGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTgg                                                                           >  2:306995/1‑135 (MQ=255)                                                              ccAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGc                                                                          <  2:304661/132‑1 (MQ=255)                                                              ccAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGc                                                                          >  1:304661/1‑132 (MQ=255)                                                                   tCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGGCGGGAGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGGCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGGATTGCTGAAGCGTTGGCCCCAGTCg                                                                  <  4:33544/135‑1 (MQ=255)                                                                   tCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGAGTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCg                                                                  <  4:27647/135‑1 (MQ=255)                                                                   tCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCg                                                                  <  4:456410/135‑1 (MQ=255)                                                                   tCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCg                                                                  <  2:193849/135‑1 (MQ=255)                                                                   tCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCg                                                                  <  3:31811/135‑1 (MQ=255)                                                                      aGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCgg                                                                 <  5:416030/133‑1 (MQ=255)                                                                      aGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGc                                                                <  4:306813/134‑1 (MQ=255)                                                                       gCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGGCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCtt                                                              <  5:53607/135‑1 (MQ=255)                                                                         gggAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGc                                                            <  5:421245/135‑1 (MQ=255)                                                                         gggAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGc                                                            >  6:141003/1‑135 (MQ=255)                                                                           gAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCTTCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTc                                                          <  2:188450/135‑1 (MQ=255)                                                                           gAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTc                                                          <  3:163169/135‑1 (MQ=255)                                                                               cGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATc                                                                                             <  2:366455/96‑1 (MQ=255)                                                                               cGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATc                                                                                             >  1:366455/1‑96 (MQ=255)                                                                               cGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCCGTCGGCTTGCTCGtcc                                                      >  5:64742/1‑133 (MQ=255)                                                                                            cgtTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCATCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTgaga                                           >  7:433315/1‑133 (MQ=255)                                                                                            cgtTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTgtga                                           >  7:418274/1‑133 (MQ=255)                                                                                                 cgccgGGTGACTTGTATGTCCTGCTGCACCAGCTTCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGt                                                                    >  3:399293/1‑103 (MQ=255)                                                                                                 cgccgGGTGACTTGTATGTCCTGCTGCACCAGCTTCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGt                                                                    >  3:406196/1‑103 (MQ=255)                                                                                                 cgccgGGTGACTTGTATGTCCTGCTGCACCAGCTTCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGt                                                                    <  4:399293/103‑1 (MQ=255)                                                                                                 cgccgGGTGACTTGTATGTCCTGCTGCACCAGCTTCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGt                                                                    <  4:406196/103‑1 (MQ=255)                                                                                                  gccgGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGtt                                    <  7:39455/134‑1 (MQ=255)                                                                                                      ggTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCg                                                                  >  2:350185/1‑100 (MQ=255)                                                                                                      ggTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCg                                                                  <  1:350185/100‑1 (MQ=255)                                                                                                      ggTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTAcc                               <  3:254710/135‑1 (MQ=255)                                                                                                      ggTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTAcc                               <  6:130169/135‑1 (MQ=255)                                                                                                      ggTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTAcc                               <  1:156790/135‑1 (MQ=255)                                                                                                         gACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCa                             >  5:460984/1‑134 (MQ=255)                                                                                                               tATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCATCACtt                       <  7:134946/134‑1 (MQ=255)                                                                                                                    ccTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCATCACTTcaccac                 >  1:287413/1‑135 (MQ=255)                                                                                                                         tgcACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTAcc                               >  7:95783/1‑116 (MQ=255)                                                                                                                         tgcACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTAcc                               <  8:95783/116‑1 (MQ=255)                                                                                                                         tgcACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCATCACTTCACCAcgcgcg            >  6:249360/1‑135 (MQ=255)                                                                                                                         tgcACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCATCACTTCACCAcgcgcg            >  1:251955/1‑135 (MQ=255)                                                                                                                           cACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCATCACTTCACCACCCgcggc          >  7:187215/1‑135 (MQ=255)                                                                                                                           cACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCGTGCTCGTACCCCTTGTGAGAGAAGGTTTTTCCAATCACTTCACCACGCgcggc          >  2:169347/1‑135 (MQ=255)                                                                                                                            aCCAGCTGCTTGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCATCACTTCACCACGCgcgg           <  6:81934/133‑1 (MQ=255)                                                                                                                              cAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGGCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCATCACTTCACCACGCGCGGcgtc       <  6:30304/135‑1 (MQ=255)                                                                                                                                gctgctGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCATCACTTCACCACGCGCGGcgtcgt     <  1:306995/135‑1 (MQ=255)                                                                                                                                gctgctGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCATCACTTCACCACGCGCGGcgtcgt     <  2:169266/135‑1 (MQ=255)                                                                                                                                   gctgGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTGGTACCCCTTGTGAGAGAAGGGTTTACCCATCACTTCACCACGCGCGGCGTCGTccc  >  8:260170/1‑134 (MQ=255)                                                                                                                                   gctgGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCATCACTTCACCACGCGCGGCGTCGTCCa  >  5:227703/1‑135 (MQ=255)                                                                                                                                   gctgGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCGTGTGAGAGAAGGTTTTACCCATCACTTCACCCCGCGCGGCGTCGTCCa  >  8:451962/1‑135 (MQ=255)                                                                                                                                   |                                                                                                                                       TGGTAAGGGTTGGCACGCAGCAGGCTGTCGACGAACTGATGCAGGCGCGGCTTGAGCGCGGCCAGCTCCAGCGGGAAGCGGTAGGCAGCGTCGTTCCGCCGGGTGACTTGTATGTCCTGCTGCACCAGCTGCTGGCTCGCCATCTGCCCCCAGTAACGCGTCAGCTCGTCCATCGCATTGCTGAAGCGTTGGCCCCAGTCGGCTTGCTCGTACCCCTTGTGAGAGAAGGTTTTACCCATCACTTCACCACGCGCGGCGTCGTCCA  >  ACB122/3473191‑3473455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑