| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,491,319 | A→C | 35.7% | intergenic (‑681/+40) | CJ019_03306 ← / ← CJ019_03307 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,491,319 | 0 | A | C | 35.7% | 10.0 / 2.6 | 31 | intergenic (‑681/+40) | CJ019_03306/CJ019_03307 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/13); new base C (9/2); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.78e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01 | |||||||||||
GCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCC > ACB122/3491194‑3491453 | gCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCGGCCTGGCcg > 6:174743/1‑135 (MQ=255) ccaaCATCGACCGGGGGTGCCGCCACGTGGTCGGAGCTGGCTAACCCGCGAATAGGCCGGACCGGCCGCCCACTACCCCTGGTCATCCCCCGACACCCCCGCCCCCCCATTCGCGGCCAGCCTGCCCGCGAacac < 6:278513/132‑1 (MQ=19) tCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGGCGCCGGCCCGCGCAcccccccgc > 3:211312/1‑130 (MQ=21) cGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCGGCCCCCGAACACCCGCGCa > 7:241269/1‑135 (MQ=255) ggtggtGTCGCCACGTTGTAGGCGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCCCCGTCACAGCCGCCCCCGCATTCGCGGGCCGCCGGCCCGCGCACCCCCGGGCCGCCgg > 6:29353/1‑135 (MQ=18) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCGCTATCCCTGGTCATTCACCGTCACAGCCGCCCCCGCATTAGCCGGCCGCCGGGCCGCGCCCACCCGCgccgcccg > 7:339651/1‑133 (MQ=18) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGGCGCCGGCCCGCCAACACCGgcgccccccg > 4:385874/1‑129 (MQ=21) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGGCGCCGGCCCCCGAACCCCCGCGCCGCCgg > 2:138148/1‑135 (MQ=21) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGGAGCCAGCCCGCGCACACCGGCGCAGCCgg > 1:153419/1‑135 (MQ=255) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCa > 8:144402/1‑107 (MQ=255) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCa < 7:144402/107‑1 (MQ=255) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCGGCCCGCGAACACCGGCGCAGCCgg > 4:110454/1‑135 (MQ=255) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCCGGCAGCCTGCCCGCCAACACCGGCCCCGCCgg > 4:94133/1‑135 (MQ=255) tgtCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCCGCCCCCGCACACCCGCCCCGCCGGGTTCa > 3:342768/1‑135 (MQ=18) gCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCGGCCCGCGCACACCGGCGCCGCCGGGTTCCTCCa > 2:24002/1‑135 (MQ=21) gcgggTTTCCCCGCTAATCGGCCGGACCGGCCGGCAACTAGCCCTGTGCATGCCCCGTCCCCCCCGCCCCCCCATTCGGGGGCCGCCGGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATc < 2:45145/132‑1 (MQ=255) cTGGTTTACCCGCGAATAGGCCGTACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCg < 4:401878/135‑1 (MQ=255) gttttCCCCGCTATTAGGCCGGCCCTGCCGGCCACTATCCGTGGTCATTCACGGCCCCAGCCCCCACCGCATTCGGGGGCAGCGTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGAc < 6:457540/133‑1 (MQ=255) ggTTTACCCGCTAATTGGCCGGACCTCCCGCCAACTTTCCCTGGCAATTCACCGTCACTCCCGCCACCGCTTTGGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGAc < 3:313995/135‑1 (MQ=255) ggTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCTGCCCGCGCACACCGGCGCCGCCGGGTTCATGCAACTCAACGccccacgcc > 7:240379/1‑129 (MQ=21) cccGCTAATCGGCCGGCCCTGCCGGCAAATAGCCTTGGTATTCCCCCGACCCCGCCGCCACCGCATGCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTAc < 7:258106/135‑1 (MQ=255) cgggattAGGCCGGACGTCCCGGCCAGTTGACCTGGGCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCACCGTGCCCGGGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGc < 8:116419/129‑1 (MQ=37) aaTAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCTGCCCGCGAACACCGGCCCCGCCGGTTTCATACAACTCAACGACCCACGACCGGTACGCCAAc > 6:347505/1‑135 (MQ=25) ccTGCCGGCCAATACCCCTGGTCATACCCCGCAACCCCCCCCACCGCATGCGCGGGCAGCCTCCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCAt < 1:150551/135‑1 (MQ=255) cACTAACCCTGGACATTCACCGACACAGCGGCCCCCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCt < 4:69363/135‑1 (MQ=255) aCTACCCCTGGCAATCCCCCGCCACCCCCGCCCCCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCtt < 5:174743/135‑1 (MQ=255) cccGTCACCGCCGCCCCCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCg < 4:369363/133‑1 (MQ=255) aCCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGGTTCATCCAACGAAACGCCCAACGCCCTGTACGCAAACGTCTCTGCCATCGCTTGCCGTGCATGGCCAAATGTCgg > 2:365674/1‑134 (MQ=255) aCCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCg < 8:241269/135‑1 (MQ=255) cgcgGGCCCCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGaa < 3:385874/134‑1 (MQ=255) gcgGGCTGCCTGCCGGCCAACACCGGCGCAGCCGGTTTCATCCAACTCCACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGc < 5:164543/135‑1 (MQ=255) cgGGCAGCGTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGca < 3:94133/135‑1 (MQ=255) cgGGCAGCCTCCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGGCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGca < 7:362725/135‑1 (MQ=255) aGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACAccc < 4:110403/135‑1 (MQ=255) | GCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCC > ACB122/3491194‑3491453 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |