Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,494,162 G→A 6.7% intergenic (‑324/‑162) CJ019_03310 ← / → CJ019_03311 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,494,1620GA 6.7% 185.6 / 3.4 60intergenic (‑324/‑162)CJ019_03310/CJ019_03311hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/32);  new base A (2/2);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01

ATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGAAAGATCAGGGTTGCAGCTGAGCATCGCAAGC  >  ACB122/3494035‑3494291
                                                                                                                               |                                                                                                                                 
atatGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCatt                                                                                                                            <  6:24343/135‑1 (MQ=255)
atatGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCat                                                                                                                             >  6:1148/1‑134 (MQ=255)
  atGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCattta                                                                                                                          <  6:127768/135‑1 (MQ=255)
    gACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGAATTCTGGAATGTGAACCCTGTCGCatttatt                                                                                                                        <  7:22221/135‑1 (MQ=255)
      cATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCatttattt                                                                                                                       >  5:285201/1‑134 (MQ=255)
      cATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTGGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCatttattt                                                                                                                       >  6:217815/1‑134 (MQ=255)
           ccTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGc                                                                                                                 >  4:122164/1‑135 (MQ=255)
                cAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCatg                                                                                                              <  3:207034/133‑1 (MQ=255)
                cAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCatg                                                                                                              <  7:154516/133‑1 (MQ=255)
                 aGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCatgatg                                                                                                           >  4:464367/1‑135 (MQ=255)
                   ggCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAAAGCATGATGGc                                                                                                         <  5:143284/135‑1 (MQ=255)
                       gTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCActt                                                                                                     <  2:357921/135‑1 (MQ=255)
                        tATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCActtt                                                                                                    >  6:107565/1‑135 (MQ=255)
                        tATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCActtt                                                                                                    >  4:259501/1‑135 (MQ=255)
                        tATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCActtt                                                                                                    >  8:375295/1‑135 (MQ=255)
                               ccGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCt                                                                                             <  3:181027/135‑1 (MQ=255)
                                cGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCGTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGACTGTGACCCCTGGCGCATTTATTTCAATGCATGATGTCCCGTTCCGTTCTg                                                                                            >  2:324055/1‑135 (MQ=255)
                                 gTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTTCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTgc                                                                                           <  5:392059/135‑1 (MQ=255)
                                     tCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCTTTTATTTCAATGCATGATGGCACATTCCTTTATgcag                                                                                         >  5:200940/1‑133 (MQ=255)
                                     tCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTTTTTAAAAGGATGATGGCACTTTCCATTTAgcaggc                                                                                       >  2:187383/1‑135 (MQ=255)
                                     tCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTgcag                                                                                         >  3:391930/1‑133 (MQ=255)
                                     tCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTAAAATCATTATGGCACTTTCCTTTATtcaggc                                                                                       >  6:265267/1‑135 (MQ=255)
                                                 aGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGttt                                                                           <  3:135161/135‑1 (MQ=255)
                                                 aGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGttt                                                                           >  2:62767/1‑135 (MQ=255)
                                                   tGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCTTTTATTTCAATGCATGATGGCCCTTTCCTTTCTGCAGGCAGTCGCATGTTTcc                                                                         >  7:44894/1‑135 (MQ=255)
                                                   tGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTcc                                                                         <  3:122164/135‑1 (MQ=255)
                                                       ttGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCaa                                                                     <  8:44324/135‑1 (MQ=255)
                                                       ttGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCaa                                                                     <  6:285201/135‑1 (MQ=255)
                                                       ttGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCaa                                                                     <  2:213966/135‑1 (MQ=255)
                                                        tGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATTCATGATGGCACATTACTTTATGCAGGAAGTAGCATGTTTCCGCaa                                                                     >  4:330853/1‑134 (MQ=255)
                                                          aCAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGc                                                                   <  4:219607/134‑1 (MQ=255)
                                                          aCAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGc                                                                   <  1:62767/134‑1 (MQ=255)
                                                             gggCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATTATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGt                                                               >  8:255963/1‑135 (MQ=255)
                                                                       tGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTTCGTTATgc                                                      <  6:44003/134‑1 (MQ=255)
                                                                          cGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATgcgcgc                                                  <  8:354293/135‑1 (MQ=255)
                                                                          cGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATgcgcgc                                                  <  5:1148/135‑1 (MQ=255)
                                                                          cGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATgcgcgc                                                  <  3:370096/135‑1 (MQ=255)
                                                                          cGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATGCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATgcgcgc                                                  <  7:309405/135‑1 (MQ=255)
                                                                          cGGTGCTTCCTTTGGTCCGTTCTGCCTGGCTGGCATTCTGGAATGTGAACGCTGTCGCATATCTTTCAATGCATGATGGCACTTTCCTTGCTGCAGGCAGTCGCATGATTCCGCAAGCACGTGCGTTATgcgcgc                                                  <  5:135601/135‑1 (MQ=255)
                                                                           ggTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCa                                                 >  6:422754/1‑135 (MQ=255)
                                                                           ggTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTATCGCATTTATTTCAATGCa                                                                                                                <  1:158380/72‑1 (MQ=255)
                                                                           ggTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTATCGCATTTATTTCAATGCa                                                                                                                >  2:152145/1‑72 (MQ=255)
                                                                           ggTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTATCGCATTTATTTCAATGCa                                                                                                                >  2:158380/1‑72 (MQ=255)
                                                                           ggTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTATCGCATTTATTTCAATGCa                                                                                                                <  1:152145/72‑1 (MQ=255)
                                                                                ttCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCa                                             >  2:20817/1‑134 (MQ=255)
                                                                                         gCCTTTCTGGCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGcc                                    <  2:127591/134‑1 (MQ=255)
                                                                                          ccTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCTTTTTTTTAAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTACGCAAGCCCGTGCGTTATGCGCGCATGCAGAATACGccc                                   >  1:421281/1‑134 (MQ=255)
                                                                                            tttCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGaa                                >  6:127212/1‑135 (MQ=255)
                                                                                               cTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGaaa                               <  3:259501/133‑1 (MQ=255)
                                                                                               cTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGaaa                               <  4:391930/133‑1 (MQ=255)
                                                                                               cTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGaaa                               <  5:107565/133‑1 (MQ=255)
                                                                                               cTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGAAAg                              <  3:398678/134‑1 (MQ=255)
                                                                                               cTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGAAAg                              <  7:375295/134‑1 (MQ=255)
                                                                                               cTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGAAAg                              <  7:96705/134‑1 (MQ=255)
                                                                                                tGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGAAAGa                             >  3:228125/1‑134 (MQ=255)
                                                                                                       ctggcATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCATGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATAAGCACTAAAGATCAggg                       <  7:207655/133‑1 (MQ=255)
                                                                                                       ctggcATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGAAAGATCAggg                       <  2:421281/133‑1 (MQ=255)
                                                                                                        tggcATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTATGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGAAAGATCAGGGTTg                    >  5:98693/1‑135 (MQ=255)
                                                                                                             ttCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGAAAGATCAGGGTTGCAGCt               <  7:255963/135‑1 (MQ=255)
                                                                                                                          aCCCTGTCGCATTTATTTAAATGCATGGTTGCACTTTCCTTTGAGCAGGCAGTCGCATGTTTACCCCAGCACGTGCGTTATGAGCGCATGCAGAATACGCCCGAAAGATCACGGTTTCCTCATCGCATCGCAAgc  >  6:262328/1‑135 (MQ=255)
                                                                                                                               |                                                                                                                                 
ATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGCACGTGCGTTATGCGCGCATGCAGAATACGCCCGAAAGATCAGGGTTGCAGCTGAGCATCGCAAGC  >  ACB122/3494035‑3494291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑