Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,584,770	G→T	6.8%	W303C (TGG→TGT)	CJ019_03397 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,584,770	0	G	T	6.8%	178.7 / 4.3	59	W303C (TGG→TGT)	CJ019_03397	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/27);  new base T (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-01

TTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCGCTGGCGAACTGGACAGATCGCT  >  ACB122/3584642‑3584903                                                                                                                                 |                                                                                                                                      ttCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTc                                                                                                                                 <  7:270953/135‑1 (MQ=255)         tGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGcc                                                                                                                         >  8:368332/1‑135 (MQ=255)            cTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCtt                                                                                                                       <  4:77751/134‑1 (MQ=255)                  gCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTg                                                                                                                >  1:132615/1‑135 (MQ=255)                  gCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTg                                                                                                                >  2:10922/1‑135 (MQ=255)                  gCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGATCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTg                                                                                                                >  6:21409/1‑135 (MQ=255)                      tATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGa                                                                                                            >  5:45667/1‑135 (MQ=255)                        tGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAgg                                                                                                          <  6:11000/135‑1 (MQ=255)                         gACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAgg                                                                                                          <  6:45667/134‑1 (MQ=255)                         gACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAgg                                                                                                          <  8:48370/134‑1 (MQ=255)                         gACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAgg                                                                                                          <  4:154648/134‑1 (MQ=255)                         gACCAGATTGGCGACGCACTGGTGGTCACAGACCTGCGCTTGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAgg                                                                                                          <  1:6268/134‑1 (MQ=255)                         gACCAGATTGGCGACGCACTGGTGGTCACAGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAgg                                                                                                          <  1:14357/134‑1 (MQ=255)                             aGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCg                                                                                                     >  2:198669/1‑135 (MQ=255)                                ttGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGc                                                                                                  >  7:78269/1‑135 (MQ=255)                                  ggCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGc                                                                                                  <  3:108870/133‑1 (MQ=255)                                  ggCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGc                                                                                                  >  4:108870/1‑133 (MQ=255)                                  ggCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCt                                                                                                >  7:317725/1‑135 (MQ=255)                                            tggtggTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGt                                                                                      <  3:178399/135‑1 (MQ=255)                                            tggtggTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGt                                                                                      >  6:409796/1‑135 (MQ=255)                                            tggtggTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGt                                                                                      >  2:235131/1‑135 (MQ=255)                                                 gtCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCGGGTCAAc                                                                                 >  1:236453/1‑135 (MQ=255)                                                   cACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGc                                                                               <  7:260311/135‑1 (MQ=255)                                                       gACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCaa                                                                           <  7:160932/135‑1 (MQ=255)                                                       gACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCaa                                                                           <  5:10432/135‑1 (MQ=255)                                                           tGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGGAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGc                                                                       <  2:176945/135‑1 (MQ=255)                                                           tGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGc                                                                       <  1:312652/135‑1 (MQ=255)                                                           tGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGATCAAGAGc                                                                       >  3:91188/1‑135 (MQ=255)                                                                aTGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATg                                                                  >  3:336291/1‑135 (MQ=255)                                                                          cGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGc                                                        >  5:35090/1‑135 (MQ=255)                                                                           ggggAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCg                                                       <  8:78269/135‑1 (MQ=255)                                                                             ggAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGAc                                                     <  6:35090/135‑1 (MQ=255)                                                                              gAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGGGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACg                                                    <  7:347147/135‑1 (MQ=255)                                                                                            gCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACca                                        >  5:403424/1‑133 (MQ=255)                                                                                            gCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACca                                        >  8:200772/1‑133 (MQ=255)                                                                                            gCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACca                                        >  3:435233/1‑133 (MQ=255)                                                                                            gCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTCGATGGTCGGAGTGCCTTCGGCGTGGCGACGCAGCGGGCCTGGCTCGCAGTTCATCGCTCAACAGCTGATGCTTATCTGGCGACGCATTGTCGACcagc                                      >  5:208579/1‑135 (MQ=255)                                                                                             cTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACcagca                                     >  7:250129/1‑135 (MQ=255)                                                                                              ttCAACATGGCTCAGCGCGATCATCAAGGGCAGTGTATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACcagcag                                    >  7:186803/1‑135 (MQ=255)                                                                                              ttCAACATGGCTCAGCGCGATCATCAAGGGCAGTGTATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACcagcag                                    >  7:181191/1‑135 (MQ=255)                                                                                                     tGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGGGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCAt                             <  6:403424/135‑1 (MQ=255)                                                                                                      gccTCAGCGCGATCAACAAGGGCAGTCCTAGCTCGGTCAGCCTTCCGCGTGGCGAGCCAGCGCGCCTGGCTCGCTGTTCAACGCTGAAGAGCTCATCCTTAACTGCCGACGCAATGACGACCAGCAGCCGCCAtt                            >  3:230432/3‑135 (MQ=255)                                                                                                        cTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTg                           <  1:19640/134‑1 (MQ=255)                                                                                                         tCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGcc                         >  5:382666/1‑135 (MQ=255)                                                                                                         tCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGcc                         >  4:130375/1‑135 (MQ=255)                                                                                                           aGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGTGCCTCGCTCGCTGTTCAACGCTAAACAGCTGATGCTTATCAGTCGACGCATTGTCCAACAGCAGCCGCCCTAGCCGc                       >  5:12135/1‑135 (MQ=255)                                                                                                           aGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGCGCCTGGCTCGCTGTTCAACGCTCAAGAGCACATGCTTATCTGGCGACGCATTGTCGACCAGCAGACGCCATTGCCGc                       >  1:264112/1‑135 (MQ=255)                                                                                                            gcgcGATCATCAAGGGCAGTGTATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCg                        <  8:181191/133‑1 (MQ=255)                                                                                                            gcgcGATCATCAAGGGCAGTGTATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCg                        <  8:186803/133‑1 (MQ=255)                                                                                                            gcgcGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCg                        <  7:168405/133‑1 (MQ=255)                                                                                                            gcgcGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCg                        <  4:429266/133‑1 (MQ=255)                                                                                                              gcgATCAGCAAGGTCAGTGGATGGTCGCTGTGCCATCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGATCTGATGCTTATCTCGCGACGCATTGTCGACCAGCAGCCGCCATTGCCGcatg                    >  2:446466/1‑132 (MQ=255)                                                                                                               cgATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCGCTGGc                   <  1:1224/135‑1 (MQ=255)                                                                                                               cgATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCACAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCGCTGGc                   <  2:305133/135‑1 (MQ=255)                                                                                                                gATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCGCTGGc                   >  6:237005/1‑134 (MQ=255)                                                                                                                     tcaCGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCGCTGGCGAACt              <  1:306961/134‑1 (MQ=255)                                                                                                                     tcaAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCGCTGGCGAACt              <  8:250129/134‑1 (MQ=255)                                                                                                                      caAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCGCTGGCGAACTgg            <  8:54577/135‑1 (MQ=255)                                                                                                                             aGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCGCTGGCGAACTGGACAGATc     >  5:207870/1‑135 (MQ=255)                                                                                                                                ggATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCGCTGGCGAACTGGACAGATCGCt  >  5:268270/1‑135 (MQ=255)                                                                                                                                 |                                                                                                                                      TTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGTGTGCCTTCGGCGTGGCGAGGCAGCGGGCCTGGCTCGCTGTTCAACGCTCAAGAGCTGATGCTTATCTGGCGACGCATTGTCGACCAGCAGCCGCCATTGCCGCTGGCGAACTGGACAGATCGCT  >  ACB122/3584642‑3584903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑