Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,957,240 C→T 100% G137R (GGG→AGG)  dmlR_13 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,957,2400CT100.0% 302.4 / NA 82G137R (GGG→AGG) dmlR_13HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/42);  total (40/42)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGAT  >  ACB122/3957111‑3957371
                                                                                                                                 |                                                                                                                                   
gcTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCgg                                                                                                                                <  5:32260/135‑1 (MQ=255)
 cTCCGGTACACGGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGc                                                                                                                               <  7:337517/135‑1 (MQ=255)
    cGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAg                                                                                                                            >  5:218132/1‑135 (MQ=255)
      gTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                          <  5:461429/135‑1 (MQ=255)
      gTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                          <  4:145120/135‑1 (MQ=255)
                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCCCCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                               >  1:256474/1‑135 (MQ=255)
                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCCCGTCATGCCTTTCGGCCAGCGGGTCGGGGGTg                                                                                                               >  1:347732/1‑135 (MQ=255)
                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                               >  8:397638/1‑135 (MQ=255)
                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                               >  3:281674/1‑135 (MQ=255)
                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                               >  4:155308/1‑135 (MQ=255)
                    tCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGCTCGGGGTTgcgc                                                                                                            >  8:205497/1‑135 (MQ=255)
                    tCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGAGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTATCGGCCAGCGGGTCGGGGTTgcgc                                                                                                            <  8:7133/135‑1 (MQ=255)
                     ccAGGTAGCCGGTGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTgcgc                                                                                                            <  8:326/134‑1 (MQ=255)
                      cAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTg                                                                                                          <  7:355309/135‑1 (MQ=255)
                       aGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCCCCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGc                                                                                                         >  5:256213/1‑135 (MQ=255)
                       aGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGc                                                                                                         >  6:182831/1‑135 (MQ=255)
                       aGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCAAGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGc                                                                                                         >  8:239046/1‑135 (MQ=255)
                                     cgcgCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGTGCTGCGACAGCGTCAGCt                                                                                            >  7:366394/1‑134 (MQ=255)
                                      gcgcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCg                                                                                          >  1:38387/1‑135 (MQ=255)
                                      gcgcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCg                                                                                          >  8:41617/1‑135 (MQ=255)
                                       cgcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGa                                                                                         <  8:308969/135‑1 (MQ=255)
                                        gcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                        <  5:177744/135‑1 (MQ=255)
                                        gcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                        <  7:325534/135‑1 (MQ=255)
                                          aGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACCGCGTCAGCTCGATca                                                                                      >  8:175201/1‑135 (MQ=255)
                                             ggATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcacca                                                                                   <  7:100086/135‑1 (MQ=255)
                                             ggATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcacca                                                                                   <  8:272100/135‑1 (MQ=255)
                                               aTGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACt                                                                                 <  7:397638/135‑1 (MQ=255)
                                                    gAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                            >  4:218534/1‑135 (MQ=255)
                                                      aaaTATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                            <  2:24037/133‑1 (MQ=255)
                                                       aaTATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGt                                                                          <  2:412282/134‑1 (MQ=255)
                                                       aaTATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTa                                                                         <  1:399618/135‑1 (MQ=255)
                                                        atatTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTaa                                                                        <  7:28640/135‑1 (MQ=255)
                                                         tatTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTaaa                                                                       >  3:285548/1‑135 (MQ=255)
                                                              cccAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtg                                                                  >  6:265499/1‑135 (MQ=255)
                                                                cAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGGCCGCTCGATCCCCACTTCCGGGTAACGCAggtt                                                                >  5:313806/1‑135 (MQ=255)
                                                                    ggCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAg                                                            >  7:284397/1‑135 (MQ=255)
                                                                      cacaGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCCCCACTTCCGGGTAAAGCATGTTGTAGcc                                                          >  5:50287/1‑135 (MQ=255)
                                                                              tCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCg                                                                             <  3:291997/108‑1 (MQ=255)
                                                                              tCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCg                                                                             >  4:291997/1‑108 (MQ=255)
                                                                                   tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                             >  3:378957/1‑135 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCGAGCGGGTCGGGGGTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                        <  4:114925/135‑1 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGTGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                        <  8:366394/135‑1 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                        <  6:230555/135‑1 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                        <  6:142505/135‑1 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                        <  5:265499/135‑1 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                        <  8:99574/135‑1 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                        <  5:173067/135‑1 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                        <  4:55773/135‑1 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                        <  4:281674/135‑1 (MQ=255)
                                                                                        aTCCGGCAGCGCACAGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                        <  2:436276/135‑1 (MQ=255)
                                                                                          ccGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCa                                      >  3:287767/1‑135 (MQ=255)
                                                                                          ccGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCa                                      <  4:287767/135‑1 (MQ=255)
                                                                                               aGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                             <  2:303586/123‑1 (MQ=255)
                                                                                               aGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                             >  1:303586/1‑123 (MQ=255)
                                                                                               aGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACGTCCGGGTAAAGCATGTTGTAGCCGGCCCCCCGCGGCATCAGCAACAGc                                 >  7:412358/1‑135 (MQ=255)
                                                                                                 cgcACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                               <  2:38387/135‑1 (MQ=255)
                                                                                                           aCGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCCCCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGc                     >  1:452638/1‑135 (MQ=255)
                                                                                                           aCGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGc                     <  5:259945/135‑1 (MQ=255)
                                                                                                           aCGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGc                     <  5:72201/135‑1 (MQ=255)
                                                                                                            cGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                    <  6:218132/135‑1 (MQ=255)
                                                                                                            cGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                    <  6:174497/135‑1 (MQ=255)
                                                                                                            cGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                    <  1:16429/135‑1 (MQ=255)
                                                                                                               tGAGCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGc                 <  5:240693/135‑1 (MQ=255)
                                                                                                                gATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCa                <  1:64814/135‑1 (MQ=255)
                                                                                                                gATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCa                >  3:371212/1‑135 (MQ=255)
                                                                                                                   caccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                        >  8:140172/1‑108 (MQ=255)
                                                                                                                   caccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                        <  7:140172/108‑1 (MQ=255)
                                                                                                                   caccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGt              >  8:229011/1‑134 (MQ=255)
                                                                                                                    accacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCCTGCCGTGCGGGCAGTg             >  6:258285/1‑134 (MQ=255)
                                                                                                                    accacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTg             >  3:189685/1‑134 (MQ=255)
                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc            >  5:308064/1‑134 (MQ=255)
                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc            >  4:190598/1‑134 (MQ=255)
                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCa           >  6:239049/1‑135 (MQ=255)
                                                                                                                       acGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCa                                      >  6:430523/1‑106 (MQ=255)
                                                                                                                       acGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCa                                      <  5:430523/106‑1 (MQ=255)
                                                                                                                       acGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCaaa         >  7:328551/1‑135 (MQ=255)
                                                                                                                          tCATGCCTTTCGGCCAGCGGGTGGGGGTTGCGCTGCGACAGCGGCAGCTCGATCACCACTTCCGCGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGc      <  5:269962/135‑1 (MQ=255)
                                                                                                                            aTGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCCCTTCCGGGTAAAGCATGTTGTAGCCGGCCCCCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAc        >  6:116141/1‑131 (MQ=255)
                                                                                                                            aTGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGc                     >  8:200285/1‑118 (MQ=255)
                                                                                                                            aTGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGc                     <  7:200285/118‑1 (MQ=255)
                                                                                                                              gCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGAt  >  7:289880/1‑135 (MQ=255)
                                                                                                                              gCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGAt  >  7:380637/1‑135 (MQ=255)
                                                                                                                                 |                                                                                                                                   
GCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGAT  >  ACB122/3957111‑3957371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑