| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB122 | 44,301 | (T)9→8 | intergenic (+132/‑359) | oprD_1 → / → czcR_2 | Porin D/Transcriptional activator protein CzcR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 44,293 | 0 | T | . | 88.3% | 202.2 / 29.6 | 62 | intergenic (+124/‑367) | oprD_1/czcR_2 | Porin D/Transcriptional activator protein CzcR |
| Reads supporting (aligned to +/- strand): ref base T (5/2); new base . (22/31); total (29/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGG > ACB122/44162‑44412 | ttCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGGCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttt < 3:344664/134‑1 (MQ=255) tGTAATTCGCTAACTGGGACAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACATTGAGTGTCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTTTCGCCCATTGGGCGCCtttttttt > 8:268743/1‑136 (MQ=255) aaTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttttttttgc > 4:25844/1‑133 (MQ=255) ccAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAg < 6:363468/135‑1 (MQ=255) aGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCG‑CGTTTTTTTTGCCTAAAATTCAAGTAGGc > 4:29922/1‑135 (MQ=255) aGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCACTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGc < 6:39560/135‑1 (MQ=255) tCCATTGGCGGGGACTCTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGATTAAGTGCTCCTTTGGAAAAAGGTGGATATATGGCGCCCATTGGGCGCC‑TTTTTTTAGCCTAAAATTCAAGTAGGCGTTCg < 7:219831/135‑1 (MQ=255) aTTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGAtt < 4:175767/135‑1 (MQ=255) gCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcg > 8:219210/1‑136 (MQ=255) gggTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTAAAGTAGGCGTTCGATTGTAcgca > 8:253349/1‑136 (MQ=255) ggTCCTGTTAGTAAAACGAGCATTACGGATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgca < 5:237484/135‑1 (MQ=255) ggTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgca > 5:152747/1‑135 (MQ=255) ccTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTGTTTTTTTGCTTATAA‑TCAAGTAGGCGTGCGCTTGTTcgcaaagc > 7:391590/1‑132 (MQ=255) cTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGGCTAAAATTAAAGTTGTCGTTGGATTGTAagcatccg > 3:368825/1‑133 (MQ=255) tGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATTTTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAA‑TAAATTAGGCGTTAGATTTTTcgcaacgcc > 6:48632/1‑134 (MQ=255) tGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAg < 4:105813/110‑1 (MQ=255) tGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAg > 3:105813/1‑110 (MQ=255) tGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcatcgcat > 7:106757/1‑136 (MQ=255) gTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTT‑CCTAAAAATCAAGTAGTCGTTCGATTGTACGCATAGGATGCCg > 5:340040/1‑135 (MQ=255) gTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg < 8:326794/135‑1 (MQ=255) aCGAGCTTTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAATAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGaa < 3:176484/135‑1 (MQ=255) aCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGaa < 2:87186/135‑1 (MQ=255) aCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAAGCATCGCCTGCCGGTGaa > 3:46487/1‑135 (MQ=255) aGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt < 1:152327/135‑1 (MQ=255) cATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCa < 5:358831/135‑1 (MQ=255) ttACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa < 8:87259/136‑1 (MQ=255) tACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa < 1:278723/135‑1 (MQ=255) tACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGAGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa < 6:252131/135‑1 (MQ=255) tGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑GTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGGAGTTCAAGACGACACCAATGccc > 7:127368/1‑136 (MQ=255) aGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc < 6:206282/134‑1 (MQ=255) aGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAc < 5:382430/133‑1 (MQ=255) aGTGGCTTAAGTGCTCATTTGGTAAAAGGTGGATATTTTGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGTCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc < 4:92783/134‑1 (MQ=255) gTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTTAAATTAAATTATGCGTTGGATTGTTCGCATACCATGCCGGTGAAGTTAATGACTACACCAATGAcccc > 4:31913/1‑133 (MQ=255) gTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑GTTTTTTTGCCTAAAATTAAAGTTGGCGTTCGATTTTACGCCTCGCATTCCGGTGAAGTTCATGACTACCCCAATGACCac > 4:38093/1‑134 (MQ=255) gggCTTAAGTGCGCCTTGGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGt < 5:207732/134‑1 (MQ=255) ggCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCGGTTTTTTTG‑CTAAAATTCAATAAGGGGGTGGATTGTACGCATCGCCTGCCCGTGAAGGTCATGACTACCCCAATTGCCAGtc > 1:329196/1‑135 (MQ=255) ggCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGGCTAAAATTCAAGTAGGCGTTCGTTTGTTAGCAACGCATGCCGGTGAACTTCATTACTTCACCAATGACCAGta > 6:98571/1‑134 (MQ=255) ggCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGtct < 8:311128/136‑1 (MQ=255) ggCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑GTTTTTTTGCCTAAAAATCAACTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAAAGACCAggc > 3:401578/1‑133 (MQ=255) cTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTT‑CCTAAAATTAAAGTAGGAGTTCGATTGTACTCATCGCATGCCGGTGAAGTTGATTACTACACCAAAGACCAGGctct > 7:191043/1‑136 (MQ=255) aaGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTAAAGTAGGCGTTCGATTGTTCGCCTCGCATGCCGGTGAAGTTCATTACTACACCAATGACCAGTCTCtt > 1:23838/1‑134 (MQ=255) aGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGTGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTc < 4:421630/134‑1 (MQ=255) gCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgt > 8:297821/1‑136 (MQ=255) cTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTT‑CCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgt < 8:27289/135‑1 (MQ=255) cTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTT‑CCTAAAATTCAAGTAGGCGGTCGATTGTACGCATCGCATGACGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgt > 5:257442/1‑135 (MQ=255) cTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgt < 7:219210/135‑1 (MQ=255) tCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgtg < 6:152747/135‑1 (MQ=255) tttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAg > 3:384039/1‑134 (MQ=255) tttGGTAAAAGGTGGATATTAGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAAGTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGGGACGTTAATGCCTACACCAATGACCAGGCGCTTCAATGTgcgcc > 7:433371/1‑135 (MQ=255) tGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGAC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt < 1:88240/96‑1 (MQ=255) tGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGAC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt > 2:88240/1‑96 (MQ=255) tAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCt > 1:237295/1‑135 (MQ=255) tAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCt > 4:345768/1‑135 (MQ=255) tAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTc < 8:88205/136‑1 (MQ=255) tGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTc > 2:261108/1‑114 (MQ=255) tGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTc < 1:261108/114‑1 (MQ=255) tGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTAAACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTc > 8:276214/1‑135 (MQ=255) tatTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTg < 8:103690/136‑1 (MQ=255) tatTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTAGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCATTGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCt < 2:158280/135‑1 (MQ=255) gcgcCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCATGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGcc < 7:233824/135‑2 (MQ=255) gcgcCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCgg < 8:106757/135‑1 (MQ=255) cgcCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCgg < 4:384039/134‑1 (MQ=255) cgcCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCgg < 4:275564/134‑1 (MQ=255) aTTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg < 5:337612/62‑1 (MQ=255) aTTGGGCGCC‑GTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg > 6:337612/1‑62 (MQ=255) | TTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGG > ACB122/44162‑44412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |