Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	5,756,060	(A)6→5	coding (781/834 nt)	CJ019_05400 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,756,055	0	A	.	95.7%	195.0 / 2.8	47	coding (776/834 nt)	CJ019_05400	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base . (35/9);  minor base T (0/2);  total (35/12)
Fisher's exact test for biased strand distribution p-value = 5.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATG  >  ACB122/5755929‑5756181                                                                                                                                |                                                                                                                               aaGCGCTCGAACCGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGaaaaacg                                                                                                                          <  4:225089/133‑3 (MQ=255)   gcgcgcGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACgccgcc                                                                                                                    >  8:111556/1‑136 (MQ=255)       gcgAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGCCCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGGGAAGCTGACGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCcagc                                                                                                                 <  3:424962/135‑1 (MQ=255)         gAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAgg                                                                                                              <  7:63998/136‑1 (MQ=255)           aCAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAgg                                                                                                              >  8:283368/1‑134 (MQ=255)                        gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCCCGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGACGCGCTGgccgccg                                                                                               >  7:418349/1‑136 (MQ=255)                        gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                               >  7:59477/1‑136 (MQ=255)                        gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                                >  5:84776/1‑135 (MQ=255)                        gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                                >  3:206283/1‑135 (MQ=255)                            tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGCAGCGG‑AAAAACGCCGCCCGCAGGAAGCGCTGGCcgccgccgc                                                                                           >  8:280693/1‑136 (MQ=255)                            tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                            >  5:3360/1‑135 (MQ=255)                            tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                            >  5:147819/1‑135 (MQ=255)                                 aaGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AACACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGccc                                                                                      >  8:109199/1‑136 (MQ=255)                                  aGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcg                                                                                     >  8:271091/1‑136 (MQ=255)                                      cTTGAAGACCATCGAGGAAACCCTCGACCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGTCCTCCGCCGCTGCCCGCGa                                                                                  >  4:246183/1‑135 (MQ=255)                                           aGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCCCGACCggg                                                                             >  1:259281/1‑135 (MQ=255)                                                aTCGAGGAAACCCTCGCCCGCCA‑GGCCCATGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGt                                                                        >  4:242876/1‑135 (MQ=255)                                                  cgcggAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGATGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCCTGa                                                                      >  5:291693/4‑135 (MQ=255)                                                  cGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCTGCCGCTGCCCGCGACCGGGCCCGTGAg                                                                     >  8:445964/1‑136 (MQ=255)                                                  cGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGa                                                                      >  1:170789/1‑135 (MQ=255)                                                  cGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAg                                                                     >  7:175212/1‑136 (MQ=255)                                                  cGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTgc                                                                      >  5:285845/1‑134 (MQ=255)                                                  cGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                      >  1:109577/1‑135 (MQ=255)                                                  cGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGGGa                                                                      >  1:438800/1‑135 (MQ=255)                                                  cGAGGAAACCCTCGCCCGCCA‑GGACCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                      >  5:437432/1‑135 (MQ=255)                                                   gcggAAACCCTGGCCCGCCC‑GGCCGGTGACGCTGAAGAGGCACGCAAGACGGCCCTGCGGGCGCAGCAGGAAGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                    <  7:283368/134‑1 (MQ=255)                                                    aGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                    >  3:337921/1‑135 (MQ=255)                                                        accccGCGCCCGCCA‑GCCCCGGGACGCTCCAGTCCCACCCACGAGGGCCCGGCGGGCGCAGCAGCAAG‑GGAAAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                <  3:267035/133‑1 (MQ=255)                                                         caccacgccccccATGGCCCGTCACGCTGATGAGGCACGCAATATGGCCATGGTGGCCCTGATTGAAGCTG‑AAACCCGCGGCATGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                <  2:270369/130‑1 (MQ=255)                                                            cTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGTGCCCGTGAGCCTGCagag                                                            >  5:246314/1‑135 (MQ=255)                                                            cTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGc                                                           >  7:223978/1‑136 (MQ=255)                                                             tCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGc                                                           >  8:39318/1‑135 (MQ=255)                                                                         gCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccg                                                >  5:310141/1‑135 (MQ=255)                                                                            cGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                             <  6:147819/135‑1 (MQ=255)                                                                               gAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGCGCCAGTGGAACCGCCGaa                                           >  5:375321/1‑134 (MQ=255)                                                                                 cgCTGAAGCGCCCCCCAACACGGCCCTGCGGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGATGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                        <  3:304558/134‑1 (MQ=255)                                                                                      ccgcggCACGCACGAAGGCCCTGCTGGCGCAGCAGTAAGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                  <  8:121870/132‑1 (MQ=255)                                                                                            cccGCAACACGGCCCTGCGGGCGCAGCCGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTaccac                            <  7:43173/134‑1 (MQ=255)                                                                                                     cggCCCTGCGGGCGCAGCAGCAACCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGc                    <  6:3360/134‑1 (MQ=255)                                                                                                         ccTGCTGGAGCAGCAGGAGGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCAGAGCCACTGGAACCGCCCAAAAAGGCCCGTACCACCCGCGGGCCgct                >  5:258289/1‑133 (MQ=255)                                                                                                            gctgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGCGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGtg            >  7:336440/1‑135 (MQ=255)                                                                                                            gctgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGt             >  3:219243/1‑135 (MQ=255)                                                                                                              tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCCCCCGCGGGCCGAGGGttt           >  2:380502/1‑135 (MQ=255)                                                                                                                gcgcAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGACCCGCCGAAAAACGCCCGGACCCCCCGCCGGCCGAGGGGTTcc         >  6:27790/1‑135 (MQ=255)                                                                                                                gcgcAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt           >  6:168932/1‑133 (MQ=255)                                                                                                                  gcagcaGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGc      <  8:223978/136‑1 (MQ=255)                                                                                                                    agcagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGGAGAGCCAGTGGAACCGCCGAAAAAGGTACGTACCACCCTCGGGCCGGTGGTTTGCcgcg     >  1:211628/1‑135 (MQ=255)                                                                                                                      cagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATg  <  8:59477/136‑1 (MQ=255)                                                                                                                                |                                                                                                                               AAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCA‑GGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATG  >  ACB122/5755929‑5756181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑