Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,684,6340AC47.2% 28.6 / 11.5 36P118P (CCA→CCC) CJ019_05336hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/17);  new base C (17/0);  total (19/17)
Fisher's exact test for biased strand distribution p-value = 2.00e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCCAGGACAACCAGCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCA‑GT‑GGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCG  >  ACB122/5684501‑5684760
                                                                                                                                     |                                                                                                                                
cccAGGACAACCAGCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCAc                                                                                                                                 <  5:434745/135‑1 (MQ=255)
      aCAACCAGCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCccccccccgc                                                                                                                           >  3:324724/1‑133 (MQ=255)
        aaCCAGCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTccc                                                                                                                         <  3:354130/135‑1 (MQ=255)
             gCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGCGGTGGTCCCCCCCCCAccccccc                                                                                                                     >  5:45430/1‑132 (MQ=255)
             gCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTGcccccccccccccccc                                                                                                                     >  6:181143/1‑132 (MQ=255)
             gCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTACCAGGACAGCAAGATCAACAAGCTCACCCCACCGCCTGCGGCCAGGCCCGAGGTCTTCGCCCCCCCGccccccc                                                                                                                     >  2:323441/1‑132 (MQ=255)
                 ggCCGCGGCACCCTGGACAAGTCAGCGGTGCCCAAGACCAGTTAGCTGGCGCCGTTCCAGGACAGCAAGAGCAACAAGATCACCCCACCGCGTGCGGCCAGGCCCGAGGTGGGCCCACCCCCTCCCCCGAAAAAGt                                                                                                               <  7:195940/136‑1 (MQ=255)
                  gCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCCGGCCCGAGGTGGTGCCCCCCCCCCCCCCCAAAAAGTc                                                                                                              >  8:296298/1‑136 (MQ=255)
                          aCCCTGGACAAGAACGCGGGCCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGAGCAACAAGATCACCCCACCGCCTGCGGCCTGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAACGTCGGCA‑gt‑g                                                                                                     <  1:67444/135‑1 (MQ=255)
                                 aCAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCCCCGCCTGCGGCCAGGCCCGAGGAGGTCCCACCCCCGCCCCCCCAAAAGTAGGCA‑GG‑GGTgccgc                                                                                              >  3:26925/1‑134 (MQ=255)
                                 aCAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCA‑GT‑GGTgac                                                                                                <  5:181143/133‑1 (MQ=255)
                                   aaGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCCCCGCCTGCGGCCCGGCCCGAGGTGGTCCCCCCCCCTCCCCCCCAAAAGTCGGCC‑GG‑GGGggcgacc                                                                                            >  6:297588/1‑134 (MQ=255)
                                     gAACGCGGTGCCCAAGACCCCTGCGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAACGGCGGCA‑GT‑GGTGACGACGgc                                                                                          <  5:376719/135‑1 (MQ=255)
                                        cgCGGTGCCCAAGCCCTCTGAGCGGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCA‑GT‑GGTGACGACGGCGccc                                                                                      <  7:394524/135‑1 (MQ=255)
                                         gCGGGGCCCAAGACCACTGCGCGGGCGCCGTTCCAGCACACCAACAACAACAACAACACCCCACCGCCTGCGCCCAGGCCGGAGGTGGTCCCACCCCCTCCCCCCAAAACGTCGGCA‑GT‑GGTGACGACGGCGccc                                                                                      <  4:238663/135‑1 (MQ=255)
                                           ggTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGCTCACCCCCCCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCGCCCCCCACAAAGTCGGCA‑GT‑GGTGGCGCCGGCGcccccg                                                                                   >  7:288551/1‑133 (MQ=255)
                                             tGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTGCCCCCCCCTCCCCCCCAAAAATCGGCA‑CT‑GGGGTCGCCGGCGccccacc                                                                                  >  2:158704/1‑131 (MQ=255)
                                                ccAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCA‑GT‑GGTGACGACGGCGCCCAAGCCGc                                                                               <  4:201977/135‑1 (MQ=255)
                                                  aaGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCACCCCCCCAAAAGTCGGCA‑GG‑GGTGACGCCGGCGCCCCAGCCGcc                                                                              >  3:350474/1‑133 (MQ=255)
                                                            aGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCTCCCCCGACAAAGTGGGCA‑GT‑GGGGGCGACGGCGCCCACGCCGCCCACGGTCgcgc                                                                   >  6:278010/1‑135 (MQ=255)
                                                            aGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCACCCCCCAAAAAAGCGGCACGT‑GGT‑ACGCCGGCGCCCAACCCCCCCAAAGtggcgc                                                                   >  2:406006/1‑130 (MQ=255)
                                                                    ccGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCCGGCCCGAGGTGGTCCCCCCCCCGCCCCCCAAAAACTGGGCG‑GT‑GGTGGCGACGGCGCCCAAGCCGCCCAAGGGGGAGCCCACAcc                                                            >  4:189241/1‑134 (MQ=255)
                                                                         ccAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCTCCCCCCAAAAAGTCGGCA‑GT‑GGTGCCGCCAGCGCCCCAGCCGCCCCACGTCGCGCCCCAACCCAAGGa                                                      >  7:43363/1‑135 (MQ=255)
                                                                         ccAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCACCCCCCAAAAAGGCGGGC‑GTGGGTGA‑GCCGGGCCCCCACCCGCCCCACGGCGAGCCCACACCCaccgg                                                      >  7:360731/1‑131 (MQ=255)
                                                                          cAGGACAGCAAGATCAACAAGATCACCCCACCGCGTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCA‑GT‑GGTGACGACGGCGCACAAGCCGCACAAGGTCGAGCCCAAACCCAAGGa                                                      <  2:22777/134‑1 (MQ=255)
                                                                           aGGACAGCAAGAGAAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCA‑GT‑GGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGaaa                                                    <  5:383020/135‑1 (MQ=255)
                                                                                aGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCA‑GT‑GGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAg                                               <  5:119707/135‑1 (MQ=255)
                                                                                     gATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCGCCCCCCCAAAAATCGGCA‑GG‑GGGGTCGCCCGCGCCCCCGCCGCCCCCCGGCGCGCCCCACCCCCACGCAAGCCAGgcgccg                                         >  8:93400/1‑134 (MQ=255)
                                                                                     gATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCCCCCCCCAAAAAGTAGGGA‑GT‑GGGGGCGACCGCGCCCCAGCCGCACAAGGGCGAGCCCAAAACCAAGGCAAGCAACGCGCac                                         >  7:174546/1‑135 (MQ=255)
                                                                                                        ccGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCA‑GT‑GGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGa                      <  7:359285/136‑1 (MQ=255)
                                                                                                         cGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCGCCCCCGCAAAAGTCGGCA‑GT‑GGGGCCGACGGCGCCCCAGCCGCCCCAGGGCGCGCCCAAACCCAAGGCAAGCAACGCGCCGCCCCAGCCCGCCCCCCCgcc                     >  8:247698/1‑134 (MQ=255)
                                                                                                         cGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCA‑GT‑GGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGa                      <  6:379482/135‑1 (MQ=255)
                                                                                                           ccTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCGCCCCCGCAAAAGTCGGCA‑GT‑GGGGGCGCCGGCGCCCAAGCCGCCCCAGGTCGCGCCCCAACCCAAGGCAACCAACGCGCCGCCCCAGCCCCCCGCGccgccg                    >  6:183133/1‑135 (MQ=255)
                                                                                                              gCGGCCAGGCCCGAGGTGGTCCCCCCCCCGCCCCCGCAAAACTCGGCA‑GT‑GGTGCCGCCGGCGCCCCCGCCGCCCCACGTCCCGCCCAAACCCCAGGAAAGCCAGGCCCCGCCCCACCCACCCCCGCCGCCCCCg                 >  1:6262/1‑135 (MQ=255)
                                                                                                              gCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCA‑GT‑GGTGACGCCGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCACGGCGCAGCCCCCGCCCGCCCCGCCGACGCCgg                >  7:194921/1‑136 (MQ=255)
                                                                                                                           aGGTGGGCCCACCCCCTCCCCCGAAAAAGTCGGCA‑GG‑GGGGACGACGGCGCCCAAGCCGCACAAGGGCGAGCCCAAACCCAAGGAAACCAAGGCGCAGCCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTc   <  7:119195/136‑1 (MQ=255)
                                                                                                                             gtggcCCCACCCCCTCCCCCGAAAACGTCGGAA‑GT‑CGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCg  <  1:50138/135‑1 (MQ=255)
                                                                                                                             ggggtCCCACCCCCTCCCCGGAAAAAGTCGGCA‑GT‑GGGGACGAGGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCg  <  6:403775/133‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                
CCCAGGACAACCAGCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCA‑GT‑GGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCG  >  ACB122/5684501‑5684760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑