Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 1,804,932 C→T S181N (AGC→AAC)  dmlR_4 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,804,9320CT97.6% 264.9 / NA 83S181N (AGC→AAC) dmlR_4HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (39/41);  minor base G (0/2);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCACTGCCTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGCTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGTCCATGCCT  >  ACB122/1804801‑1805060
                                                                                                                                   |                                                                                                                                
ccAGCACTGCCTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTtgt                                                                                                                               >  2:121986/1‑135 (MQ=255)
          cTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                     <  3:311380/135‑1 (MQ=255)
          cTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                     >  3:278034/1‑135 (MQ=255)
          cTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                     <  1:98839/135‑1 (MQ=255)
          cTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGCTGTGTggccggcc                                                                                                                     <  4:31403/135‑1 (MQ=255)
            ggCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCCCGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                     >  4:199981/1‑133 (MQ=255)
            ggCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                     >  3:300072/1‑133 (MQ=255)
            ggCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCAt                                                                                                                   >  4:34568/1‑135 (MQ=255)
               cACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGTCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAgc                                                                                                                <  5:445939/135‑1 (MQ=255)
               cACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAgc                                                                                                                <  4:303682/135‑1 (MQ=255)
               cACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAgc                                                                                                                <  3:126426/135‑1 (MQ=255)
               cACTGTTGCAGCGCCAGTTACCCTCTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGGGGCCGGCCATAgc                                                                                                                <  1:4084/135‑1 (MQ=255)
                    ttGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGATCTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCt                                                                                                             >  3:409202/1‑133 (MQ=255)
                     tGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCa                                                                                                           <  5:142381/134‑1 (MQ=255)
                          cgcCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAgg                                                                                                    <  7:300463/136‑1 (MQ=255)
                               gTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGc                                                                                               >  7:30954/1‑136 (MQ=255)
                                ttACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCg                                                                                              <  7:12661/136‑1 (MQ=255)
                                 tACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCg                                                                                              <  1:86166/135‑1 (MQ=255)
                                    ccTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGaa                                                                                            <  3:34568/134‑1 (MQ=255)
                                     cTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAac                                                                                          <  5:446608/135‑1 (MQ=255)
                                     cTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATACGCCGGCGAAac                                                                                          <  6:302976/135‑1 (MQ=255)
                                       gTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAacac                                                                                        >  4:302319/1‑135 (MQ=255)
                                       gTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAacac                                                                                        >  3:42509/1‑135 (MQ=255)
                                           ccGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGACACACACAg                                                                                    >  1:210671/1‑135 (MQ=255)
                                           ccGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAg                                                                                    >  6:396607/1‑135 (MQ=255)
                                             gctggctgATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                     <  6:85442/100‑1 (MQ=255)
                                             gctggctgATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                     >  5:85442/1‑100 (MQ=255)
                                               tggctgATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTAc                                                                                <  3:302319/135‑1 (MQ=255)
                                                 gctgATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACAt                                                                              >  4:8910/1‑135 (MQ=255)
                                                    gATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATgcg                                                                           >  1:420447/1‑135 (MQ=255)
                                                     aTCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAacacac                                                                                      >  5:181025/1‑123 (MQ=255)
                                                     aTCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAacacac                                                                                      <  6:181025/123‑1 (MQ=255)
                                                     aTCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGCTACATgcgc                                                                          >  1:344827/1‑135 (MQ=255)
                                                     aTCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCACGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATgcgc                                                                          >  2:99247/1‑135 (MQ=255)
                                                               cATCCTGCTGTAGCGCCCACAAGTCCGCACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCa                                                                >  1:247888/1‑135 (MQ=255)
                                                                   ctgctgTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGGGTGGCCGGCCATAGCGCTCAACATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCg                                                            <  1:426626/135‑1 (MQ=255)
                                                                   ctgctgTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGGGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCg                                                            <  1:426623/135‑1 (MQ=255)
                                                                    tgctgTAGCGCCCACAAGTCCGAACTCCCCACCAGGCATTTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCgg                                                           <  5:5711/135‑1 (MQ=255)
                                                                    tgctgTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCggg                                                          >  8:398660/1‑136 (MQ=255)
                                                                    tgctgTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCgg                                                           >  2:93092/1‑135 (MQ=255)
                                                                    tgctgTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCgg                                                           <  5:409120/135‑1 (MQ=255)
                                                                    tgctgTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACAAGCGCCTAGGCGCCAGGCgg                                                           >  4:374808/1‑135 (MQ=255)
                                                                    tgctgTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGCGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCgg                                                           <  1:37337/135‑1 (MQ=255)
                                                                          aGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGc                                                      <  4:353326/134‑1 (MQ=255)
                                                                           gcgcCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTgg                                                                     >  4:402198/1‑118 (MQ=255)
                                                                           gcgcCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTgg                                                                     <  3:402198/118‑1 (MQ=255)
                                                                             gcCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGccacc                                                  <  3:199981/135‑1 (MQ=255)
                                                                              cccACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGccacca                                                 >  6:150520/1‑135 (MQ=255)
                                                                                 acaAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAgg                                               >  3:362852/1‑134 (MQ=255)
                                                                                 acaAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGc                                              >  2:288846/1‑135 (MQ=255)
                                                                                 acaAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGc                                              >  8:278536/1‑135 (MQ=255)
                                                                                    aGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCg                                             >  4:226294/1‑133 (MQ=255)
                                                                                    aGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCg                                             >  1:151542/1‑133 (MQ=255)
                                                                                          aaCTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGTCAGTCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCCCCAGGCGCGAGTCgg                                     >  1:333789/1‑135 (MQ=255)
                                                                                            cTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCgg                                     >  6:371669/1‑133 (MQ=255)
                                                                                             tCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCa                                  >  4:191509/1‑135 (MQ=255)
                                                                                             tCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAg                                 >  7:347905/1‑136 (MQ=255)
                                                                                                ccaccaGGCAATTGTGCCGCGCCAGCTCCGACAGGGTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTGGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAggc                               <  3:199030/135‑1 (MQ=255)
                                                                                                  acccGGCAATGGTGCCGCGCCAGCTCCGCCCGGGTGTGGGGCCGGCCATAGCGCTCAAGAGAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAggcggc                            <  8:185374/136‑1 (MQ=255)
                                                                                                  accaGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAggcgg                             >  4:204461/1‑135 (MQ=255)
                                                                                                  accaGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAggcgg                             >  3:186351/1‑135 (MQ=255)
                                                                                                  accaGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAggcgg                             >  6:299483/1‑135 (MQ=255)
                                                                                                   ccaGGCACTTGGGCCGCGCCCGCTCCGACAGGTTGTGGGGCCGGCCATAGCGCTCAAGCTCGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAggcggc                            <  1:374714/135‑1 (MQ=255)
                                                                                                    caGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAggcggc                            >  6:360728/1‑134 (MQ=255)
                                                                                                       gCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGa                       <  8:140649/136‑1 (MQ=255)
                                                                                                       gCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGa                       <  7:398660/136‑1 (MQ=255)
                                                                                                        cAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGa                       <  6:191154/135‑1 (MQ=255)
                                                                                                        cAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGa                       <  4:278034/135‑1 (MQ=255)
                                                                                                        cAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGa                       <  3:418014/135‑1 (MQ=255)
                                                                                                          aTTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGAc                      >  3:312745/1‑134 (MQ=255)
                                                                                                             gtgCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGAt                  <  7:172168/135‑1 (MQ=255)
                                                                                                              tgCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATg                 >  6:263228/1‑135 (MQ=255)
                                                                                                              tgCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATg                 <  7:278536/135‑1 (MQ=255)
                                                                                                              tgCCGCGCCAGCGCCGACAGGTTGTGGGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATg                 <  1:320412/135‑1 (MQ=255)
                                                                                                               gCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATg                 <  6:29282/134‑1 (MQ=255)
                                                                                                               gCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATg                 <  4:384351/134‑1 (MQ=255)
                                                                                                                    gcCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAgg           >  6:190104/1‑135 (MQ=255)
                                                                                                                    gcCAGCTCCGACAGGTTGGGTGGCCGGCCATAGCGCTCAAGAGAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACTGATGGCCAGGt          <  7:227394/136‑1 (MQ=255)
                                                                                                                     cccgCTCGGACCGGTTGTGTGGCCGGCCATAGCGCTCAAGCTAGGCCGGCTAAACACACAGGTACAGGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGt          <  5:254724/132‑1 (MQ=255)
                                                                                                                     ccAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGt          <  4:362852/135‑1 (MQ=255)
                                                                                                                     ccAGCTCCGACAGGTTGTGGGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGt          <  5:190104/135‑1 (MQ=255)
                                                                                                                          tCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGTCCATg     <  3:8910/135‑1 (MQ=255)
                                                                                                                             gACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGTCCATGCCt  <  1:121986/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                
CCAGCACTGCCTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGCTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGTCCATGCCT  >  ACB122/1804801‑1805060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: