Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 3,957,240 C→T G137R (GGG→AGG)  dmlR_13 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,957,2400CT97.3% 259.4 / ‑1.9 73G137R (GGG→AGG) dmlR_13HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (38/33);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

GCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAC  >  ACB122/3957111‑3957365
                                                                                                                                 |                                                                                                                             
gcTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGc                                                                                                                         <  7:403172/136‑1 (MQ=255)
 cTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGc                                                                                                                         <  1:269757/135‑1 (MQ=255)
  tCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCCCGTCATGCCTTTCGGc                                                                                                                         >  8:135347/1‑134 (MQ=255)
  tCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCgg                                                                                                                          >  1:246807/1‑133 (MQ=255)
     ggTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                    <  7:426733/136‑1 (MQ=255)
               gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                           >  6:403541/1‑135 (MQ=255)
               gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                           >  1:270041/1‑135 (MQ=255)
                ggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGtt                                                                                                          <  3:317617/135‑1 (MQ=255)
                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACTCCGATCAGTTGCGAATCCGTCAGCGCACCGTCCACGGTGATCAGCACGTCATGCCTTTCGGCCTGCGGGTCGGGCTTg                                                                                                         >  6:271813/1‑135 (MQ=255)
                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTgc                                                                                                        >  7:240063/1‑136 (MQ=255)
                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTgc                                                                                                        >  8:60789/1‑136 (MQ=255)
                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTgc                                                                                                        >  8:402027/1‑136 (MQ=255)
                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                         >  3:60343/1‑135 (MQ=255)
                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                         >  2:362194/1‑135 (MQ=255)
                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                         >  2:265326/1‑135 (MQ=255)
                   gtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTgcg                                                                                                       >  4:287664/1‑135 (MQ=255)
                   gtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTgcg                                                                                                       >  3:208144/1‑135 (MQ=255)
                      cAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTg                                                                                                    <  4:245072/135‑1 (MQ=255)
                      cAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGATGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGGTGCGCTg                                                                                                    <  7:445996/135‑1 (MQ=255)
                       aGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGc                                                                                                   >  5:438131/1‑135 (MQ=255)
                       aGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGc                                                                                                   >  2:387527/1‑135 (MQ=255)
                        ggTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCg                                                                                                  >  1:251782/1‑135 (MQ=255)
                         gTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGa                                                                                                 >  1:364675/1‑135 (MQ=255)
                              cGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCg                                                                                            >  5:130732/1‑135 (MQ=255)
                               gggggCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCg                                                                                            >  3:57434/1‑134 (MQ=255)
                                 gggCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCa                                                                                         >  6:367169/1‑135 (MQ=255)
                                 gggCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCa                                                                                         <  1:23776/135‑1 (MQ=255)
                                       cgcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGa                                                                                   <  4:218695/135‑1 (MQ=255)
                                             ggATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcaccac                                                                            <  8:75240/136‑1 (MQ=255)
                                              gATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcaccac                                                                            <  2:270041/135‑1 (MQ=255)
                                                   tGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCg                                                                       <  3:287664/135‑1 (MQ=255)
                                                    gAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCggg                                                                     >  8:405303/1‑136 (MQ=255)
                                                    gAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                      >  1:321359/1‑135 (MQ=255)
                                                       aaTATTGCCCAGCGGCACAGCGATCAGTTGCGAATGCGGCAGCGCACCGTCGACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGt                                                                    <  3:346068/134‑1 (MQ=255)
                                                         tatTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAg                                                                >  8:349932/1‑136 (MQ=255)
                                                               ccAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtgtt                                                          >  7:294350/1‑136 (MQ=255)
                                                               ccAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtgtt                                                          >  7:79888/1‑136 (MQ=255)
                                                               ccAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtgt                                                           >  4:383615/1‑135 (MQ=255)
                                                                cAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtgtt                                                          >  1:396872/1‑135 (MQ=255)
                                                                    ggCACAGCGAGCAGTGGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAg                                                      <  1:175375/135‑1 (MQ=255)
                                                                      cacaGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCt                                                                                                     <  1:213997/86‑1 (MQ=255)
                                                                      cacaGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCt                                                                                                     >  2:213997/1‑86 (MQ=255)
                                                                             aTCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGccacca                                            <  7:59949/136‑1 (MQ=255)
                                                                              tCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGccacca                                            <  5:231907/135‑1 (MQ=255)
                                                                                 gTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGc                                          <  2:364675/134‑1 (MQ=255)
                                                                                       aaTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                  <  8:176347/136‑1 (MQ=255)
                                                                                       aaTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                  <  7:402027/136‑1 (MQ=255)
                                                                                       aaTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                  <  7:31251/136‑1 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCCCCAGCGGCATCAGc                                 >  7:308724/1‑136 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                  <  4:349402/135‑1 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTa                                                                   >  5:43182/1‑102 (MQ=255)
                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTa                                                                   <  6:43182/102‑1 (MQ=255)
                                                                                          ccGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCa                                <  4:60343/135‑1 (MQ=255)
                                                                                                gcgcACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                         <  7:16876/136‑1 (MQ=255)
                                                                                                gcgcACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                         <  8:265271/136‑1 (MQ=255)
                                                                                                 cgcACCTTCCACGGTGATCACCACGGCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                         <  5:170497/135‑1 (MQ=255)
                                                                                                 cgcACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                         <  1:79531/135‑1 (MQ=255)
                                                                                                 cgcACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                         <  5:403541/135‑1 (MQ=255)
                                                                                                   cACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCa                       <  3:383615/135‑1 (MQ=255)
                                                                                                    aCCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGc                                                                                       <  4:396744/70‑1 (MQ=255)
                                                                                                    aCCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGc                                                                                       >  3:396744/1‑70 (MQ=255)
                                                                                                           aCGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg              <  7:349932/136‑1 (MQ=255)
                                                                                                           aCGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACACCCCCATGCCTTGCg              >  8:334573/1‑136 (MQ=255)
                                                                                                           aCGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGGAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGc               >  4:184844/1‑135 (MQ=255)
                                                                                                           aCGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCGGCGACAGCGGCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg              <  8:15263/136‑1 (MQ=255)
                                                                                                                   caccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTg       <  3:543/135‑1 (MQ=255)
                                                                                                                   caccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTg       <  2:246807/135‑1 (MQ=255)
                                                                                                                    accacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTg       >  5:7894/1‑134 (MQ=255)
                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc      >  6:263199/1‑134 (MQ=255)
                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc      >  6:219888/1‑134 (MQ=255)
                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc      >  2:232830/1‑134 (MQ=255)
                                                                                                                            aTGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAc  >  4:427240/1‑131 (MQ=255)
                                                                                                                            aTGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAc  >  1:254606/1‑131 (MQ=255)
                                                                                                                                 |                                                                                                                             
GCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAC  >  ACB122/3957111‑3957365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑