Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 5,756,060 (A)6→5 coding (781/834 nt) CJ019_05400 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,756,0550A.97.7% 195.2 / ‑1.2 45coding (776/834 nt)CJ019_05400hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (34/9);  minor base C (0/1);  total (34/11)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGC  >  ACB122/5755925‑5756183
                                                                                                                                  |                                                                                                                                
cTGCAAGCGCGCTAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGaaaaacg                                                                                                                            <  7:53953/137‑3 (MQ=255)
 ggCAAGCGCGCGAACAGGCCCAGCCCGACCTGGCCAAGGGCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGaaaaacgc                                                                                                                           <  4:62562/136‑4 (MQ=255)
     aGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACgccgcc                                                                                                                      >  3:176297/1‑137 (MQ=255)
      gcgcgcGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGCCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCca                                                                                                                     >  6:109431/1‑137 (MQ=255)
          gcgAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCcagcag                                                                                                                 >  5:82181/1‑137 (MQ=255)
          gcgAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCcagcag                                                                                                                 >  7:90308/1‑137 (MQ=255)
                cggACCCGCCCGACCGGGCCAAGGTCTTTAAGACCATCGAGGAAACCCTCGCCCCCCAGCCCCGGGAAGCGGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAgcg                                                                                                           <  5:167399/136‑1 (MQ=255)
                             ccTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGCAGCGG‑AAAACCGCCGCAAGCAGGACGCGCCGGccgccgccg                                                                                              >  4:9696/1‑137 (MQ=255)
                               tGGCCAAGGTCTTGAAGACCATCGAGGAACCCCTCGCCCGCCAGGCCCGTGACGCTGATGCGGCACGCAAGACGGCCCTGCTGGCGCAGAAGGAAGCGG‑CAAAACGCCGACAGCAGGAAGCTCTGGACGACGCCGCt                                                                                            >  1:294454/1‑137 (MQ=255)
                               tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCt                                                                                            >  1:57830/1‑137 (MQ=255)
                               tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCt                                                                                            >  5:357168/1‑137 (MQ=255)
                                              aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGcc                                                                             >  3:250962/1‑137 (MQ=255)
                                              aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                              >  4:259176/1‑136 (MQ=255)
                                              aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                              <  3:259176/136‑1 (MQ=255)
                                                 ccATCGAGGAAACCCTCCCCCGCCCGGCCCGTGACGGTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGAAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCGt                                                                          <  6:78125/137‑1 (MQ=255)
                                                 ccAGCGAGGAAACCGTCCCCCGCCCGGCCGGGGAACCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCTCAGAAGTAAGCGG‑AAACCCGCGGCCAGCTGGAAGCGCTGGCGGCCGCCGCTGCCCGCGACCGGGCCCGt                                                                          <  6:63770/137‑1 (MQ=255)
                                                    tCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTgcg                                                                       >  6:205092/1‑135 (MQ=255)
                                                     cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      >  3:24347/1‑137 (MQ=255)
                                                     cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      >  2:230585/1‑137 (MQ=255)
                                                     cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      >  1:57391/1‑137 (MQ=255)
                                                     cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCCGCCCGCGCCCGGGCCCgggcgc                                                                      >  7:156426/1‑132 (MQ=255)
                                                     cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCCGCCCGCGACCGGGCCCGTGAGc                                                                      >  7:161534/1‑137 (MQ=255)
                                                       aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGccg                                                                    >  7:312747/1‑136 (MQ=255)
                                                       aGGAAACCCTCGCCCGCCAGGCCCGTCAAGCTGAAGAGGCACGCAAGAAGGCCCTGCAGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCt                                                                    >  8:355778/1‑137 (MQ=255)
                                                                 cgcccgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAg                                                          >  4:178834/1‑137 (MQ=255)
                                                                     cgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGa                                                      >  6:24070/1‑137 (MQ=255)
                                                                           gCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgcc                                                >  4:59651/1‑137 (MQ=255)
                                                                                tGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaa                                           >  3:307596/1‑137 (MQ=255)
                                                                                 caaGCTCAACCGGCCCGCAAGAAGGCCCGGCTGGCGCAGCAGGAAGC‑GCAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                          <  4:176297/136‑1 (MQ=255)
                                                                                   aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAgg                                        >  2:11994/1‑137 (MQ=255)
                                                                                   aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAgg                                        >  6:62740/1‑137 (MQ=255)
                                                                                       gAAGAGGCACGCAAGACGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                    <  1:278667/137‑1 (MQ=255)
                                                                                         acagGCACGCAAGATGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTa                                  <  8:101483/135‑1 (MQ=255)
                                                                                           agGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGACCGTGAGCCTGCAGAGCCAGTCGAACCGCCGAAAAAGGCCCGTacc                                >  1:45294/1‑137 (MQ=255)
                                                                                                cGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      <  6:54362/94‑1 (MQ=255)
                                                                                                cGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      >  5:54362/1‑94 (MQ=255)
                                                                                                cGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCt                           >  6:329779/1‑137 (MQ=255)
                                                                                                  aaagacgGCCCTGCTGGCGCAGCAGGATG‑GGGAAAAACGCCGCCAGCAGGAAGGGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCg                         <  5:109431/136‑1 (MQ=255)
                                                                                                   aagaagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCgg                        >  8:128054/1‑137 (MQ=255)
                                                                                                     gaagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCCCCCTCGGGc                      >  4:42609/1‑137 (MQ=255)
                                                                                                     gaagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGc                      <  6:62948/137‑1 (MQ=255)
                                                                                                                tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGACCCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTcc           >  1:112148/1‑137 (MQ=255)
                                                                                                                tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTcc           >  4:125304/1‑137 (MQ=255)
                                                                                                                tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTcc           >  2:6078/1‑137 (MQ=255)
                                                                                                                      agcagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGAt     >  7:49409/1‑137 (MQ=255)
                                                                                                                      agcagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACACTCGGGCCGCTGGTTTCCAGCGAt     >  8:117016/1‑137 (MQ=255)
                                                                                                                         agGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGgc  <  1:207716/137‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                
CTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGC  >  ACB122/5755925‑5756183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑