| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,542,102 | 0 | T | G | 53.3% | ‑0.3 / 13.7 | 17 | intergenic (+31/‑214) | CJ019_01454/groS | hypothetical protein/10 kDa chaperonin |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (8/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAAC‑GTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGCGG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAGGTGGTTCGCTCACAGC > ACB122/1541971‑1542230 | gcCAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAAC‑GTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCCACCGG‑GCCC‑GGggtttt > 7:26004/1‑137 (MQ=255) aTTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAAC‑GTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCCAGCGGCCCCC‑GGTTTTTTTTTgccggg > 2:331911/1‑132 (MQ=255) aTTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAAC‑GTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGCGG‑GCGC‑GGGTTTTTTTTGGccggg > 6:243580/1‑134 (MQ=255) tCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAAC‑GTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCCCCACCCG‑CCCC‑GGGTTTTTTTTGGGCggatt > 1:53219/1‑136 (MQ=255) ccccTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAAA‑GTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGCGG‑CCCC‑GGGTGTTTTTTTGCGTGATGAAAAAgttta > 2:288391/1‑136 (MQ=255) cTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAAC‑GTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCCAGGGG‑CCCCGGGTTTTTTTTTGGCGTGTTTAAAAttttgtat > 4:35113/1‑133 (MQ=255) cTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAAC‑GTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGCGG‑CCCC‑GGGTTTTTTTTGGGCGGATGAAAAATTTTatat > 4:39625/1‑137 (MQ=255) cTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAAC‑GTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGCGG‑CCCC‑GGGTTTTTTTTGGGCGGATGAAAAATTTTGTAt > 7:170373/1‑137 (MQ=255) cTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAAC‑GTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGCGG‑CCCC‑GGGTTTTTTTTGGGCGGATGAAAAATTTTGTAt > 4:82296/1‑137 (MQ=255) ccAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAAC‑GTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGGCCCGCCAGCGGCCCCC‑GGTTTTTTTTTGGCGGGTGTAAAAAtttttttatt > 3:329453/1‑131 (MQ=255) gcAGCCGCGTGATGGCGGCCACCGCCCCAAC‑GTGAACGAAGGCGAGAACGAGCGCCGCGACAAGTAAGAAGGACACGGGCCGCCCAACCCG‑CACC‑CGATATTTTTTGGCCATCAGTAAAATTTTGATTATCGAGCCt > 8:244959/1‑137 (MQ=255) gggcCCCCCCCCGCACGGTGAGCGATGGCGAGACCGAGCGCCGCCAAAAGAAACAAGCCCCGGGCCCGCCCACGCGG‑CCCC‑GG‑TTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTgg < 1:35644/136‑1 (MQ=255) cccccccAAC‑GTCATCCACGGCGCGTACGCGCCCCGCGACAATAAACACGCCCCGGGGCCGCGAACGCGG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCt < 5:343609/134‑1 (MQ=255) ccacggTGAGCGACGGCTAGTCCGCGCGCCGCAACAACTACCAGCCACGGGGCCCGCGCACGCGG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCatgt < 8:149650/132‑1 (MQ=255) aCGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGCGG‑CCCC‑GGGTTTTTTTTTGGCTGATGTAAAATTTTGTTTGTCGAGCCTTGTTATGGGGTGTGGCGGGCGGATGTTAGTGGTACCCGCAAGGtttt > 7:345011/1‑136 (MQ=255) aCGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGCGG‑CCCC‑GGGTTTTTTTTGGGCGGATGTAAAATTTTCTTTGTCGGGCCGTGTAATCGGGTTTTGGGGGCGGATGTTTTTGGTGACCGCAAGGtttt > 5:355314/1‑136 (MQ=255) attacgaagGCCGGGGCCCGCGCAAGCGG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCTAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAg < 2:18838/132‑1 (MQ=255) gccccc‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAGGTGGTTCGCTCACAGc < 1:331911/135‑1 (MQ=255) | GCCAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAAC‑GTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGCGG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAGGTGGTTCGCTCACAGC > ACB122/1541971‑1542230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |