Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 690,144 A→G intergenic (+21/‑2438) bauC → / → PP_RS08750 Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122690,1440AG92.9% 13.3 / ‑5.8 15intergenic (+21/‑2438)bauC/PP_RS08750Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base G (13/0);  minor base T (1/0);  total (15/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCC  >  ACB122/690028‑690236
                                                                                                                    |                                                                                               
ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGTGGCCGGCAGGCCTCCCCGcg                                                                            >  8:95947/1‑136 (MQ=255)
ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGAGGCCGTCCCTCCGCCCCGcc                                                                            >  8:265723/1‑136 (MQ=255)
              taCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGCTGCCGCCCCGCGTTGTTTTTtggggg                                                            >  4:199450/1‑136 (MQ=255)
               aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCCGGCAGGCCGCCCCCCCTTTGTTTTTTGGggt                                                            >  7:341682/1‑137 (MQ=255)
                      gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGTCAGGCCGCCCCCCGTTGGTTTTGGGGGGTGggtgtt                                                    >  8:252427/1‑137 (MQ=255)
                      gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGAGGGCGGCCCGCGGTGGTTTTTGGGGGTGggtgtt                                                    >  6:59790/1‑137 (MQ=255)
                      gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGAGGGCGCCCCGCGGTGGTTTTTGGGggttggggtt                                                     >  2:229102/1‑133 (MQ=255)
                      gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGAGGGCACCCCCCGTTGTTTTTTGGGGGTGgtggtt                                                    >  8:202475/1‑137 (MQ=255)
                      gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCCGGGACGGCCCGCCCCGTTGTTTTTTTGGGGTGggtgtt                                                    >  7:331172/1‑137 (MQ=255)
                      gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCCGGCACGCCACCCCCCCTTGTTTTTTGGGGGTGgttgtt                                                    >  8:316700/1‑137 (MQ=255)
                        aaGGCCATTTCCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCCCGGGGGGCTCCCCCCGGTGTTTTTTGGGGGTGggtgtggg                                                  >  2:199646/1‑134 (MQ=255)
                        aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCGGGGCGGGCGGCCCCCGTTGTTTTTTGGGGGTGGttgttgg                                                  >  6:239627/1‑136 (MQ=255)
                        aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGCCGGGAAGGCGCACCCCTGTGTTTTTTTGGGGTGGgttgtgg                                                   >  4:230906/1‑135 (MQ=255)
                                        gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGCCCGCCGGCCCGCCGGGGTTTTTGGGGGTGGTTGGTGTGTTGgggggggggggg                                 >  5:312652/1‑131 (MQ=255)
                                                                        cAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGTGAGGGCGCCCCCCGGTGGTTTTTGGGGGTGGGTGTTTGTGGTTGGGGGGTAAAGGTTTTTGGGGGGGGTTGGTGTGTGAtttttga  >  8:101703/1‑131 (MQ=255)
                                                                                                                    |                                                                                               
GGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCC  >  ACB122/690028‑690236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: