Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	ACB122	4,257,352	+GCGGCCGC	intergenic (‑281/+7)	CJ019_03991 ← / ← CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	ACB122	= 4257351	0 (0.000)	46 (1.000) +CGCGGCCG	32/242	0.2	100%	intergenic (‑280/+8)	CJ019_03991/CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein
	?	ACB122	4257352 =	0 (0.000)					intergenic (‑281/+7)	CJ019_03991/CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein

GGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB122/4257216‑4257351 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTGGC  >  ACB122/4257352‑4257480                                                                                                                                         ||||||||                                                                                                                                  GGCGCCCCATCACTGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCC                                                                                                                                          <  2:125017/137‑1      CCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGC                                                                                                                                     <  5:183255/137‑1       CCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    <  8:68241/137‑1       CCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    <  4:202170/137‑1       CCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    <  2:234835/137‑1       CCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    <  8:164566/137‑1       CCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    <  8:160431/137‑1       CCAACAATGGATCCACTAAAAACCGATAAGCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    <  5:229392/137‑1         ATCAATGGATCCACTAAAACCCGATAAACGCACACAATCCTCACATACCTAACAAAATGCATTCACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGC                                                                                                                                  <  1:296958/137‑1                   CCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTAC                                                                                                                        >  4:125897/1‑137                   CCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTAC                                                                                                                        <  2:162406/137‑1                     ACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACACCGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCATACGA                                                                                                                      >  5:263726/1‑137                      CTAAAAACCGATAATCGCACAGAATCCGCACCTACCGATCAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGAC                                                                                                                     >  3:253449/1‑137                        AAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGA                                                                                                                   <  7:260134/137‑1                         AAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAG                                                                                                                  >  8:24951/1‑137                         AAAACCGAAAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGTATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAG                                                                                                                  <  1:107724/137‑1                             CCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTCCGCCGCGGGCc                                                                                                              >  1:242754/1‑136                             CCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCA                                                                                                              <  5:148197/137‑1                              CGATAATCGCACAGAATCCTCACCTACCGAACAAAATGCATTGACCTAACACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAG                                                                                                             <  6:243264/137‑1                                   ATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACAACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGGCATACGACGAGTGCAGGATGA                                                                                                        >  4:267255/1‑137                                          AGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCAT                                                                                                 >  4:109334/1‑137                                          AGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCAT                                                                                                 >  6:114481/1‑137                                          AGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTAAT                                                                                                 >  7:191300/1‑137                                                         CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGCCGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  >  4:196295/1‑137                                                         CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  >  3:240351/1‑137                                                         CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  <  7:209748/137‑1                                                         CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  >  4:30288/1‑137                                                         CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  >  3:89329/1‑137                                                         CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  >  2:10052/1‑137                                                          cAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCA                                                                                 <  2:53901/136‑1                                                          GAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCA                                                                                 <  4:179125/137‑1                                                                  TGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCC                                                                         <  8:6228/137‑1                                                                  TGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCC                                                                         <  5:189103/137‑1                                                                       TGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGG                                                                    <  8:21933/137‑1                                                                         ACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    >  3:69383/1‑71                                                                         ACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    <  4:69383/71‑1                                                                            acCCACGCCCACCGCACCCTAAAGGGACCAATAAAAAAAAAAAAAAACCCGGAGAGCCACCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCG                                                               <  1:293211/135‑1                                                                                ACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGCCTTTCATGCCCGCGTCTGCCGCCGCCCCGCCAAAGGTTTCGGCAT                                                           >  6:12763/1‑137                                                                                ACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCAT                                                           >  8:89557/1‑137                                                                                 CGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  >  4:151596/1‑113                                                                                 CGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  <  3:151596/113‑1                                                                                                     GATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGACCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCAAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGT                                      >  6:303013/1‑137                                                                                                          ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCG                                 >  6:124212/1‑137                                                                                                          ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCG                                 >  5:219700/1‑137                                                                                                          ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCG                                 >  1:267597/1‑137                                                                                                          ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCG                                 >  7:4661/1‑137                                                                                                               ATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCAT                            <  2:267597/137‑1                                                                                                                   AAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATC                        >  6:146314/1‑137                                                                                                                      CAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCGAAAGGGTTCGGGATAGCGCAAGGTGGCATTTGCGTGCTCGCGCATGATCGCT                     >  8:65931/1‑137                                                                                                                      CAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCA                                                     <  1:232896/105‑1                                                                                                                      CAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCA                                                     >  2:232896/1‑105                                                                                                                            CCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCG               >  5:186547/1‑137                                                                                                                             CGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGC              <  3:30288/137‑1                                                                                                                             CGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGC              <  7:24951/137‑1                                                                                                                                       CCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTG    <  6:124286/137‑1                                                                                                                                       CCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTG    >  6:54254/1‑137                                                                                                                                        CCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTGG   <  1:126931/137‑1                                                                                                                                         CGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTGGC  >  2:108178/1‑137                                                                                                                                         ||||||||                                                                                                                                  GGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB122/4257216‑4257351 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTGGC  >  ACB122/4257352‑4257480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.