Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 5,226,693 G→A I91I (ATC→ATT)  CJ019_04921 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,226,6930GA97.3% 119.9 / ‑4.0 37I91I (ATC→ATT) CJ019_04921hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (25/11);  total (25/12)
Fisher's exact test for biased strand distribution p-value = 3.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

CGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTGATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGACGAACGACAGCAGCAGGCCGGTGAGCGTGGCATCG  >  ACB122/5226568‑5226825
                                                                                                                             |                                                                                                                                    
cGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCcag                                                                                                                           <  7:249324/137‑1 (MQ=255)
   gCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCcagcag                                                                                                                        >  8:28849/1‑137 (MQ=255)
   gCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCcagcag                                                                                                                        >  6:106586/1‑137 (MQ=255)
                attgCGAACATCAGGCAGACGAAGATCAGGCCGGTGCAGGTGTGCAGCCAGACCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGcc                                                                                                           <  4:193094/134‑1 (MQ=255)
                  ggCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCaa                                                                                                         <  2:277713/137‑1 (MQ=255)
                   gCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTGATCCAGAAGCCAACGCCCCTAATCACGCCCCGCAGCAAGCTTGGCGCCaaa                                                                                                        >  4:248516/1‑136 (MQ=255)
                     gAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATAc                                                                                                      >  5:118523/1‑137 (MQ=255)
                         aacaGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTACTTTCATGCGTTTTTCATACACTAGGCCACGCCGCTAATCCCCCCCAGCAGCAGGCTTGGCGCCATTCCGccc                                                                                                  >  1:263138/1‑137 (MQ=255)
                                 gccGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCCACCAGGc                                                                                          >  3:38371/1‑137 (MQ=255)
                                   cGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCAc                                                                                        <  8:77254/137‑1 (MQ=255)
                                   cGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCAc                                                                                        <  2:32514/137‑1 (MQ=255)
                                               cGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGc                                                                            >  4:244250/1‑137 (MQ=255)
                                                ggTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCACCACCAGGCTTGGCGCCAATAAGCCCAACCAGGCACGCCAGGCGCTGCg                                                                           >  5:166881/1‑137 (MQ=255)
                                                             aGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAacac                                                              >  6:261358/1‑137 (MQ=255)
                                                               ccAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTGATCCAGTAGGCAACGCCGCTAATCACGCCCACCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAacacaa                                                            >  4:108886/1‑135 (MQ=255)
                                                               ccAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACCCCa                                                            >  3:238116/1‑137 (MQ=255)
                                                               ccAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCa                                                            >  5:158405/1‑137 (MQ=255)
                                                               ccAGGCCATGGCCTGGCGGAAGCCATCTTTCATGCGTTTTTCATCCAGTAGGCAACCCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATAAGCCCCACCCGGCCCCCCCGGCGCTGCGGCAGGCGCAACAccc                                                            >  1:295741/1‑136 (MQ=255)
                                                                        ggCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGTCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGc                                                   <  2:64532/137‑1 (MQ=255)
                                                                        ggCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTGATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGc                                                   <  2:28473/137‑1 (MQ=255)
                                                                                   aGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTaga                                        <  1:206181/137‑1 (MQ=255)
                                                                                      attcttTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGACCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGTCACGCCAGGAGCTGCGGCAGGCGAAACACCAGAAGAACGCCAGCAGGTagaaga                                     >  6:222079/2‑137 (MQ=255)
                                                                                       ttcttTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCTACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGac                                    >  6:70485/1‑137 (MQ=255)
                                                                                       ttcttTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGac                                    >  8:152977/1‑137 (MQ=255)
                                                                                       ttcttTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGac                                    >  4:196655/1‑137 (MQ=255)
                                                                                       ttcttTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGac                                    >  6:38074/1‑137 (MQ=255)
                                                                                        tcttTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGacg                                   <  6:158405/137‑1 (MQ=255)
                                                                                               tGCGTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGACGAACGAca                            <  7:28849/137‑1 (MQ=255)
                                                                                                  gTTTTTCATCCAGTATGCAACGACGCAAATCACGCCCAGCACCAGGCTTGGCGCCAATACCCCCAACCACGCACGCCAGGCGCTGCGGAAGGCGAAACACCAGATGAACGCAAGCACCAAGAAGACGAACGAcatca                         >  5:226290/1‑137 (MQ=255)
                                                                                                  gTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGACGAACGAcagca                         >  4:90581/1‑137 (MQ=255)
                                                                                                  gTTTTTCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCACGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGACGAACGAcagca                         >  4:148400/1‑137 (MQ=255)
                                                                                                    ttttCATCCAGTAGGCAACGCCGCTAATCACGCCCCGCAGCCGGCTTGGCGCCAATACGCCCACCCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGACGAACGAcagcagc                       <  4:276235/137‑1 (MQ=255)
                                                                                                       tCATCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGACGAACGACAGCAGCAgg                    >  7:289550/1‑137 (MQ=255)
                                                                                                         aTCCAGTAGGCAACGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGACGAACGACAGCAGCAGGcc                  >  7:187412/1‑137 (MQ=255)
                                                                                                                    aaCGCCGCTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGACGAACGACAGCAGCAGGCCGGTGAGCGTgg       <  3:148772/137‑1 (MQ=255)
                                                                                                                         cgcTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCACCCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGACGAACGACAGCAGCAGGCCGGTGAGCGTGGCATCg  >  4:258357/1‑137 (MQ=255)
                                                                                                                         cgcTAATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGACGAACGACAGCAGCAGGCCGGTGAGCGTGGCATCg  >  5:122594/1‑137 (MQ=255)
                                                                                                                             |                                                                                                                                    
CGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATGGCCTGGCGGAAGCCTTCTTTCATGCGTTTTTCATCCAGTAGGCAACGCCGCTGATCACGCCCAGCAGCAGGCTTGGCGCCAATACGCCCAACCAGGCACGCCAGGCGCTGCGGCAGGCGAAACACCAGATGAACGCCAGCAGGTAGAAGACGAACGACAGCAGCAGGCCGGTGAGCGTGGCATCG  >  ACB122/5226568‑5226825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑