| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,539,411 | Δ1 bp | 6.6% | coding (109/1440 nt) | dht → | D‑hydantoinase/dihydropyrimidinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,539,411 | 0 | C | . | 6.6% | 209.3 / 7.6 | 61 | coding (109/1440 nt) | dht | D‑hydantoinase/dihydropyrimidinase |
| Reads supporting (aligned to +/- strand): ref base C (32/25); new base . (2/2); total (34/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
CGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCGTTCATGGGCACGGTGGCCAGCGAGGACTTCTTCAGCGGCACCGCTGC > ACB122/4539279‑4539544 | cGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGc > 4:195255/1‑136 (MQ=255) gTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGccc < 8:322734/136‑1 (MQ=255) aGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGCTCCGTGCCATCGGGCAAAACCTCGAACCGCCCAc > 2:219747/1‑135 (MQ=255) aGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAACACCTCGACCCGCCCAcc > 3:252743/1‑136 (MQ=255) aGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCAcc > 4:84939/1‑136 (MQ=255) aGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCAcc > 3:110290/1‑136 (MQ=255) aGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCAc > 1:328522/1‑135 (MQ=255) aGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAA‑CGCCCAcc > 6:117569/1‑135 (MQ=255) aGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAA‑CGCCCAc > 6:122972/1‑134 (MQ=255) aGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGAAGGCCTGATCCGTGCCCTCGGGCAACCCCTCGAACCGCCCAc > 1:171864/1‑135 (MQ=255) aGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTA‑CCCGCCGATGTCCTGTGTGCCGCTGGCCTGCTCCCTGGCCTCGGGCAAAACCGCGCACcccccccc > 1:186808/1‑135 (MQ=255) aGCCTGCGAGGAAAACGGCATGTCCCTGGTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCAc < 1:160418/135‑1 (MQ=255) tGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACgg < 8:320923/136‑1 (MQ=255) tGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACgg < 8:302447/136‑1 (MQ=255) gTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTTTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGTACCGCCCACCGACTGCGAGATCCTCGACgg < 2:135211/135‑1 (MQ=255) tCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCa < 7:270048/136‑1 (MQ=255) ccTGTGCATCCGGGCCGCCCCCGAGGTTACCCACGAACCGAGTTACCCCGCCGAGGTCCGGGGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGc < 4:25239/136‑1 (MQ=255) gTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGCTCCGTGCCATCGGGCAACACCTCGAACCGCCCACCCACTGCGAGATCCTCGACGGCAGCGGCCAGTAcc > 5:18353/1‑135 (MQ=255) ggCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTg < 2:328522/135‑1 (MQ=255) cgcCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGc < 6:93405/121‑1 (MQ=255) cgcCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGc > 5:93405/1‑121 (MQ=255) cgcCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGAt > 6:377679/1‑135 (MQ=255) cgcCACCGTGGTTACACACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCGGATCCGTGCCCTCGGGGAAAACCACGAACCGCCCCCCGCCAGCGCGCACCACGACGGCAGCGGCCCGAAccctat > 2:379222/1‑131 (MQ=255) cACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGCCGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGAt > 6:73299/1‑135 (MQ=255) aCGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAA‑CGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCTTCGATcc < 5:122972/135‑1 (MQ=255) aCGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAA‑CGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATcc < 5:117569/135‑1 (MQ=255) cGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGCCGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATcc > 6:211190/1‑135 (MQ=255) aaGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCCCCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGc < 6:393283/135‑1 (MQ=255) agagTTCCCCCGCCGATGGCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCacac < 3:84939/136‑1 (MQ=255) agagTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGcacc > 1:205886/1‑134 (MQ=255) agTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACAccc < 3:104994/136‑1 (MQ=255) agTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACAccc < 7:74450/136‑1 (MQ=255) tACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGc < 2:313741/127‑1 (MQ=255) tACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGc > 1:313741/1‑127 (MQ=255) tACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCcac > 4:36274/1‑135 (MQ=255) ccccGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACAt > 2:242912/1‑135 (MQ=255) ccgccgATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCACGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCCCCCCCACATg > 4:132012/1‑134 (MQ=255) aTGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACCCCCACATGCAGTTGc > 1:148132/1‑135 (MQ=255) aTGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGcc > 3:200083/1‑136 (MQ=255) aTGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGc > 5:80797/1‑135 (MQ=255) aTGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGc > 1:15128/1‑135 (MQ=255) aTGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGc > 2:321163/1‑135 (MQ=255) tGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCg < 7:43892/136‑1 (MQ=255) gCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTAcc < 2:43780/82‑1 (MQ=255) gCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTAcc > 1:43780/1‑82 (MQ=255) attgCCTGATGCGTGCCATCTGGCAAAAACTCGACCCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCGTTCATGGGCACgg < 6:335051/132‑1 (MQ=255) tGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACAccc > 4:354527/1‑107 (MQ=255) tGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACAccc < 3:354527/107‑1 (MQ=255) tGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCGTTCATGGGCACGGTGGCCa < 1:242912/135‑1 (MQ=255) tCCGTGCCCTCGGGCAAAACCTCGATCCGCCCACCGCCGGCGAGATCCTCGACGGCAGCGTCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCGTTCATGGGCACGGTGGCCAGc < 2:203776/134‑1 (MQ=255) tGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCGTTCATGGGCACGGTGGCCAGCGAGGAc < 8:259060/136‑1 (MQ=255) cATCGGGCAAAACGTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGCGGCATCGCTCCGCCCCCCCACCTGCAGTTGCCGTGCATGGGCCCGGTGGACAGCGAggcgttc > 3:264195/1‑131 (MQ=255) cATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGCTCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACCCCCACATGCAGTTGCCGTTCATGGGCCCGGTGGCCAGCGAGGActtc > 3:60023/1‑136 (MQ=255) cATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCTTCGATCCGCACAACAACATGCAGTTGCCGTTCATGGGCTCGGAGGCCAGCGAgtcctt > 1:203776/1‑130 (MQ=255) cATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCg < 3:156814/106‑1 (MQ=255) cATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCg > 4:156814/1‑106 (MQ=255) cATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCGTTCATGGGCACGGTGGCCAGCGAGGActt > 1:212893/1‑135 (MQ=255) tccaaCCGCCCCCCGCCTGCGAGACCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCGTTCATGGGCACGGTGGCCAGCGAGGACTTCTTCAGCGGCACCg < 1:379222/132‑1 (MQ=255) tCGAACCGCCCCCCGACTGCGCGAGCCGCGACGGCAGCGGCCAGTACCTGAGGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCGGTCATGGGCACGGTGGCCAGCGAGGACTTCTTCAGCGGCACCg < 5:33741/135‑1 (MQ=255) aaCCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCGTTCATGGGCACGGTGGCCAGCGAGGACTTCTTCAGCGGCACCGc < 5:377679/133‑1 (MQ=255) aCCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCGTTCATGGGCACGGTGGCCAGCGACGACTTCTTCAGCGGCACCGCTGc > 1:38529/1‑135 (MQ=255) | CGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCCGCCGATGTCCTGTGTGCCGATGGCCTGATCCGTGCCATCGGGCAAAACCTCGAACCGCCCACCGACTGCGAGATCCTCGACGGCAGCGGCCAGTACCTGATGCCTGGGGGCATCGATCCGCACACCCACATGCAGTTGCCGTTCATGGGCACGGTGGCCAGCGAGGACTTCTTCAGCGGCACCGCTGC > ACB122/4539279‑4539544 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |