Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,591,760 Δ1 bp 5.8% coding (1128/1467 nt) CJ019_04341 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,591,7600T. 5.8% 228.3 / 7.7 69coding (1128/1467 nt)CJ019_04341hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/33);  new base . (2/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

TCGCGAGGGGCTCGAAAATCTGCCTGCATCCCCTGCAGCCTTAGCAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGCGGTCGCCGCACGGATGGCCTCAGCCA  >  ACB122/4591626‑4591893
                                                                                                                                      |                                                                                                                                     
tCGCGAGGGGCTCGAAAATCTGCCTGCATCCCCTGCAGCCTTAGCAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGCCCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGAtt                                                                                                                                      >  3:171471/1‑136 (MQ=255)
  gcgAGGGGCTCGAAAATCTGCCTGCATCCCCTGCAGCCTTAGCAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGc                                                                                                                                    >  7:238314/1‑136 (MQ=255)
      ggggCTCGAAAATCTGCCTGCATCCCCTGCAGCCTTAGCAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGt                                                                                                                                <  3:387989/136‑1 (MQ=255)
              aaaaTCTGCCTGCATCCCCTGCAGCCTTAGCAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGccc                                                                                                                          <  2:37239/134‑1 (MQ=255)
               aaaTCTGCCTGCATCCCCTGCAGCCTTAGCAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCt                                                                                                                        >  2:14221/1‑135 (MQ=255)
                       ctgcATCCCCTGCAGCCTTAGCAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAgccag                                                                                                               >  3:190374/1‑136 (MQ=255)
                       ctgcATCCCCTGCAGCCTTAGCAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAgcca                                                                                                                <  5:304512/135‑1 (MQ=255)
                       ctgcATCCCCTGCAGCCTTAGCAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAgcc                                                                                                                 <  6:140646/134‑1 (MQ=255)
                       ctgcATCCCCTGCAGCCTTAGCAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAgcc                                                                                                                 <  6:173324/134‑1 (MQ=255)
                                    aGCCTTAGCAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGgt                                                                                                  <  3:259232/136‑1 (MQ=255)
                                          aGCAACACCGGTTCTCCGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGTCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGtcgtcg                                                                                             <  7:142379/135‑1 (MQ=255)
                                            cAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGAc                                                                                           >  3:91573/1‑135 (MQ=255)
                                             aaCACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGAc                                                                                           >  1:263896/1‑134 (MQ=255)
                                             aaCACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGAc                                                                                           >  1:89439/1‑134 (MQ=255)
                                                   ggTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGTTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACAcc                                                                                    >  2:390428/1‑135 (MQ=255)
                                                   ggTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGt                                                                                                                                <  7:99652/91‑1 (MQ=255)
                                                   ggTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGt                                                                                                                                >  8:99652/1‑91 (MQ=255)
                                                   ggTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACAcc                                                                                    >  1:201782/1‑135 (MQ=255)
                                                   ggTTCTACGCCGCCGCTTTCCAGCTGGCGGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGGCGTCGACAAACAccc                                                                                   <  4:258377/136‑1 (MQ=255)
                                                    gTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACAccc                                                                                   <  2:277884/135‑1 (MQ=255)
                                                       cTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGcc                                                                                                                                   <  3:358412/84‑1 (MQ=255)
                                                       cTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGcc                                                                                                                                   >  4:358412/1‑84 (MQ=255)
                                                       cTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGt                                                                                                                                >  2:27705/1‑87 (MQ=255)
                                                       cTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGt                                                                                                                                <  1:27705/87‑1 (MQ=255)
                                                       cTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCACGCCAGGCATTCGTCGTCGCCCAACACCCgcc                                                                                >  2:194768/1‑133 (MQ=255)
                                                        tACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGAc                                                                                <  5:27362/134‑1 (MQ=255)
                                                          cgccgccggTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGt                                                                             <  1:185266/135‑1 (MQ=255)
                                                          cgccgacgcTTTCAAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGt                                                                             <  8:174739/135‑1 (MQ=255)
                                                                 ggtttCCAGCTTGCTGACCAGGTCTTCGCGAAGGCTGGGTTACTCTTTGGAAGCGTCGGGTTTCGCGGATTGCCAGTCTGCCCCTTTGAGGCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAg                                                                     <  8:212123/134‑1 (MQ=255)
                                                                  cTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACCACCCCCCGCCCGTCGCTGAAg                                                                     >  7:12631/1‑135 (MQ=255)
                                                                  cTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACCCCCGACCGTCGCTGaa                                                                      >  5:329945/1‑134 (MQ=255)
                                                                  cTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAg                                                                     >  7:36211/1‑135 (MQ=255)
                                                                  cTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAg                                                                     >  2:37003/1‑135 (MQ=255)
                                                                  cTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAg                                                                     >  6:20883/1‑135 (MQ=255)
                                                                  cTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGc                                                                    >  4:3652/1‑136 (MQ=255)
                                                                  cTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATGCCCGTCTGCCCCTTTg                                                                                                                     >  8:356035/1‑86 (MQ=255)
                                                                  cTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATGCCCGTCTGCCCCTTTg                                                                                                                     <  7:350417/86‑1 (MQ=255)
                                                                  cTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATGCCCGTCTGCCCCTTTg                                                                                                                     <  7:356035/86‑1 (MQ=255)
                                                                  cTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATGCCCGTCTGCCCCTTTg                                                                                                                     >  8:350417/1‑86 (MQ=255)
                                                                     tCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGca                                                                   <  8:212154/134‑1 (MQ=255)
                                                                      ccAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGca                                                                   <  2:2511/133‑1 (MQ=255)
                                                                              ctgACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCa                                                        <  8:36211/136‑1 (MQ=255)
                                                                               tgACCAGTTCTTCGCGACGGCTGGTGAACGCTTGGGACGCTTCGGCTTGCTCGGATGGCCCGTCGGCCCCTTTGAGCCAGGCCAGGCATTGGGCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCa                                                        <  1:191743/135‑1 (MQ=37)
                                                                               tgACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCa                                                        <  2:7582/135‑1 (MQ=255)
                                                                                           cgcgAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCAt                                              <  7:342780/133‑1 (MQ=255)
                                                                                             cgAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATgcgaa                                         <  7:75273/136‑3 (MQ=255)
                                                                                              gAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCgcag                                        >  7:48556/1‑136 (MQ=255)
                                                                                              gAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCgcag                                        >  7:243862/1‑136 (MQ=255)
                                                                                              gAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCgca                                         >  5:16227/1‑135 (MQ=255)
                                                                                                   cTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGgt                                                                                                  <  7:129772/73‑1 (MQ=255)
                                                                                                   cTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGgt                                                                                                  >  8:129772/1‑73 (MQ=255)
                                                                                                   cTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACg                                    >  8:191837/1‑135 (MQ=255)
                                                                                                          aCTCTTGGGAAGCTTCGGCGTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGc                            <  3:42941/136‑1 (MQ=255)
                                                                                                           ctctTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGc                            <  6:329945/135‑1 (MQ=255)
                                                                                                               tGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGCGGTc                        >  2:42094/1‑135 (MQ=255)
                                                                                                                gggAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGCGGTcg                       <  3:3652/135‑1 (MQ=255)
                                                                                                                 ggAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCg                                                                           >  1:165578/1‑82 (MQ=255)
                                                                                                                 ggAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCg                                                                           <  2:165578/82‑1 (MQ=255)
                                                                                                                 ggAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAg                                                                     >  6:31918/1‑88 (MQ=255)
                                                                                                                 ggAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAg                                                                     <  5:31918/88‑1 (MQ=255)
                                                                                                                         cGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTg                                                                        <  4:285498/77‑1 (MQ=255)
                                                                                                                         cGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTg                                                                        >  3:285498/1‑77 (MQ=255)
                                                                                                                         cGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGCGGTCGCCGCACGGa              >  6:129449/1‑135 (MQ=255)
                                                                                                                           gCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGCGGTCGCCGCACGGATgg           >  4:99400/1‑136 (MQ=255)
                                                                                                                           gCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGCGGTCGCCGCACGGATgg           >  3:321556/1‑136 (MQ=255)
                                                                                                                               gCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGCGGTCGCCGCACGGATGGCCt        <  7:303994/135‑1 (MQ=255)
                                                                                                                               gCTCGGATAGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCAGTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGCGGTCGCCGCACGGATGGCCt        <  3:277616/135‑1 (MQ=255)
                                                                                                                                   ggATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGCCCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGCGGTCGCCGCACGGATGGCCTCAg     >  1:91233/1‑134 (MQ=255)
                                                                                                                                   ggATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGCGGTCGCCGCACGGATGGCCTCAGcc   >  4:67973/1‑136 (MQ=255)
                                                                                                                                     aTTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGCGGCCGCCGCACGGATGGCCTCAGCCa  <  1:14221/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
TCGCGAGGGGCTCGAAAATCTGCCTGCATCCCCTGCAGCCTTAGCAACACCGGTTCTACGCCGCCGCTTTCCAGCTGGCTGACCAGTTCTTCGCGAAGGCTGGTGAACTCTTGGGAAGCTTCGGCTTGCTCGGATTGCCCGTCTGCCCCTTTGAGCCAGGCCAGGCATTGGTCGTCGACAAACACCCGACCGTCGCTGAAGCACAGCTGCTGCAGCGCTGGCATGCGCAGCACGAAACGAGCGGTCGCCGCACGGATGGCCTCAGCCA  >  ACB122/4591626‑4591893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: