Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	31,613	G→A	5.8%	R244Q (CGG→CAG)	oprP →	Porin P

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	31,613	0	G	A	5.8%	223.6 / 2.0	69	R244Q (CGG→CAG)	oprP	Porin P
Reads supporting (aligned to +/- strand):  ref base G (37/28);  new base A (2/2);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGGATGGCGACAGCACCAAGCGCTACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGATGGCCTGTGGAAGGACGACTCGGTC  >  ACB122/31482‑31740                                                                                                                                    |                                                                                                                                cTCGGATGGCGACAGCACCAAGCGCTACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGAtt                                                                                                                             >  8:378036/1‑136 (MQ=255)     gATGGCGACAGCACCAAGCGCTACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGAttc                                                                                                                            <  5:138149/133‑1 (MQ=255)                cACCAAGCGCTACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTggg                                                                                                               <  2:10041/135‑1 (MQ=255)                   cAAGCGCTACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTc                                                                                                           <  4:365442/136‑1 (MQ=255)                   cAAGCGCTACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTc                                                                                                           <  7:194635/136‑1 (MQ=255)                    aaGCGCTACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTcc                                                                                                          <  4:115832/136‑1 (MQ=255)                     aGCGCTACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTcc                                                                                                          <  6:147278/135‑1 (MQ=255)                       cgcTACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCcgcg                                                                                                       >  3:340023/1‑136 (MQ=255)                        gcTACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTGTGGGCGTCcgcg                                                                                                       >  5:273590/1‑135 (MQ=255)                        gcTACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCcgcg                                                                                                       >  6:129215/1‑135 (MQ=255)                          tACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGgt                                                                                                     <  5:258515/135‑1 (MQ=255)                             aaCGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTa                                                                                                  >  1:292777/1‑135 (MQ=255)                                    gtGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCaa                                                                                          >  4:181973/1‑136 (MQ=255)                                       gCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATgg                                                                                       >  7:91017/1‑136 (MQ=255)                                            ttCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGGCTGGGCGTCCGCGGTGTAGATACCAATggcggc                                                                                   <  7:171543/135‑1 (MQ=255)                                              cGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATggcggcggc                                                                                >  7:236471/1‑136 (MQ=255)                                               gCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGCCCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGGCTGGGCGTCCGCGGTGGAGATACCAATggccgcggc                                                                                >  6:221420/1‑135 (MQ=255)                                                    gCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTc                                                                                                           >  4:36223/1‑103 (MQ=255)                                                    gCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTc                                                                                                           <  3:36223/103‑1 (MQ=255)                                                                tGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTcc                                                                                                          >  4:365337/1‑92 (MQ=255)                                                                tGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTcc                                                                                                          <  3:365337/92‑1 (MQ=255)                                                                tGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACgg                                                               >  2:47795/1‑135 (MQ=255)                                                                tGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACgg                                                               >  7:293612/1‑135 (MQ=255)                                                                tGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACg                                                                >  2:224322/1‑134 (MQ=255)                                                                     aCGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATTGCGGCGGCGATGCCGGCACCAACGGCAACCg                                                         >  8:197257/1‑136 (MQ=255)                                                                     aCGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAAcc                                                          >  2:127053/1‑135 (MQ=255)                                                                      cGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGc                                                        <  3:117093/136‑1 (MQ=255)                                                                         ccTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATg                                                     >  3:176041/1‑136 (MQ=255)                                                                          cTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTcc                                                                                                          >  8:208534/1‑82 (MQ=255)                                                                          cTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTcc                                                                                                          <  7:208534/82‑1 (MQ=255)                                                                          cTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATg                                                     >  6:226324/1‑135 (MQ=255)                                                                          cTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCAGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCTCATGc                                                    >  4:106053/1‑136 (MQ=255)                                                                                gTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTg                                              >  3:150910/1‑136 (MQ=255)                                                                                     tGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCgg                                          >  5:155372/1‑135 (MQ=255)                                                                                     tGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGt                                         >  7:115049/1‑136 (MQ=255)                                                                                     tGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGt                                         >  3:27233/1‑136 (MQ=255)                                                                                        tCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGTGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGtgct                                      >  4:97196/1‑136 (MQ=255)                                                                                        tCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGtg                                        <  1:31760/134‑1 (MQ=255)                                                                                         cAGTACGCTTACCGCGACCTCAAAAACTGTGCGGTCGATACCCGGATTCGGTCGCGGCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGtgctg                                     <  3:5767/136‑1 (MQ=255)                                                                                              cGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGca                                >  3:289037/1‑136 (MQ=255)                                                                                               gCTTACCGCGACCTCAAAAACAGTGCGGTCGATAGCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGcac                               <  8:3207/136‑1 (MQ=255)                                                                                               gCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGt                                                                                                            <  3:291200/59‑1 (MQ=255)                                                                                               gCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGt                                                                                                            >  4:291200/1‑59 (MQ=255)                                                                                                cTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGcaca                              <  4:27233/136‑1 (MQ=255)                                                                                                cTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGcaca                              <  3:280956/136‑1 (MQ=255)                                                                                                    ccGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACACGAt                          <  4:340023/136‑1 (MQ=255)                                                                                                    ccGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGAt                          >  7:228799/1‑136 (MQ=255)                                                                                                     cgcgACCTCAAACCCAGTGCGGTCGATCCCCGGCTTCGGTCGCGGCTGGGCGGCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGAt                          <  5:221420/135‑1 (MQ=255)                                                                                                     cgcgACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGAt                          <  5:129215/135‑1 (MQ=255)                                                                                                      gcgACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCaa                                                                  >  5:64754/1‑94 (MQ=255)                                                                                                      gcgACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGGGTAGATACCAATGGCGGCGGCGATGCCGGCACCaa                                                                  <  6:64754/94‑1 (MQ=255)                                                                                                       cgACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGATgg                        >  5:114739/1‑135 (MQ=255)                                                                                                       cgACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGATGGc                       >  8:55836/1‑136 (MQ=255)                                                                                                       cgACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTCTTTGGCGGTGCTGCTGAACAAGATgt                        >  5:245782/1‑134 (MQ=255)                                                                                                         aCCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACg                                                                <  1:103164/93‑1 (MQ=255)                                                                                                         aCCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACg                                                                >  2:103164/1‑93 (MQ=255)                                                                                                         aCCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTg                                                  >  3:151405/1‑107 (MQ=255)                                                                                                         aCCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTg                                                  <  4:151405/107‑1 (MQ=255)                                                                                                         aCCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGATGGCCt                     >  7:116683/1‑136 (MQ=255)                                                                                                         aCCTCAAAAACAGTGCGGTCGATACCCAGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGATGGCCt                     >  3:326463/1‑136 (MQ=255)                                                                                                               aaaaCAGTGCGGTCGATACCCAGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGGGGCGGCGATGCCGGCACCAACGGCAACCGCTTGCTGTTTGGCGGTGCTGCTGCACAAGATGGCCTGTGGaa               <  3:106053/136‑1 (MQ=255)                                                                                                                   cAGTGGGGTCGATCCCCGGATTCGTTCGCGGCTGGGCGTCCGCGGTGTAGATACCATTGGGGGGGGCGATGCGGGAACCAACGGCAACCGCTGGCTTTTTGGCGGTGCTGCTGCACAAGATGGGCTGTTTAAGgac           <  4:176041/136‑1 (MQ=255)                                                                                                                    aGTGCGGTCGATACCCGGATTCGTGCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGATGGCCTGTGGAAGgacg          <  8:321448/136‑1 (MQ=255)                                                                                                                         ggTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGGGCTGCTGCACAAGATGGCCAGTGGAAGGACGCCTCg     >  3:269762/1‑136 (MQ=255)                                                                                                                         ggTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCAGGCTGGTTGGCGGTGCTGCTGCACAAGATGGCCTGTGGAAGGACGACTCg     >  8:76644/1‑136 (MQ=255)                                                                                                                           tCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGATGGCCTGTGGAAGGACGACTCGGt   <  8:116683/136‑1 (MQ=255)                                                                                                                           tCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGATGGCCTGTGGAAGGACGACTCGGt   >  3:311004/1‑136 (MQ=255)                                                                                                                            cGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGATGGCCTGTGGAAGGACGACTCGGTc  <  8:293612/136‑1 (MQ=255)                                                                                                                            cGATACCCAGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGATGGCCTGTGGAAGGACGACTCGGTc  <  4:326463/136‑1 (MQ=255)                                                                                                                                    |                                                                                                                                CTCGGATGGCGACAGCACCAAGCGCTACAACGTACGTGGCGTGTTCGCACCGCTGCACAGTGATGGCAACGTCCTGCACGTCGGTGCTCAGTACGCTTACCGCGACCTCAAAAACAGTGCGGTCGATACCCGGATTCGTTCGCGTCTGGGCGTCCGCGGTGTAGATACCAATGGCGGCGGCGATGCCGGCACCAACGGCAACCGCATGCTGTTTGGCGGTGCTGCTGCACAAGATGGCCTGTGGAAGGACGACTCGGTC  >  ACB122/31482‑31740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑