Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 478,896 T→G 30.8% D30A (GAC→GCC)  apaH_1 ← Bis(5'‑nucleosyl)‑tetraphosphatase, symmetrical

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122478,8960TG30.8% 63.9 / 5.2 52D30A (GAC→GCC) apaH_1Bis(5'‑nucleosyl)‑tetraphosphatase, symmetrical
Reads supporting (aligned to +/- strand):  ref base T (29/5);  new base G (10/7);  total (39/13)
Fisher's exact test for biased strand distribution p-value = 7.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCCGGAAGGGGGCAATCT  >  ACB122/478774‑479027
                                                                                                                          |                                                                                                                                   
gcaggtgcaggtCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACAGGCGGTCGCCGGCggggg                                                                                                                         >  5:69613/1‑134 (MQ=255)
gcaggtgcaggtCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCCGCCACAGGCGGTCGACGGCggggg                                                                                                                         >  5:182130/1‑134 (MQ=255)
      gcaggtCATGGTTGCCCCCCACGCAGCCCTGTGAATGAGGGAGCGATTCCAGGTAGCGCTGGGTTTCCAGTGCCTGGGGGCCGCGGTTGCCCCGGTCGCCGACCAGCCACCGGCGGTCGACGGCGGGGTTGAAAc                                                                                                                   <  6:174364/135‑1 (MQ=255)
                 ttGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGCCCAGGTCGCCCACCAGCCACAGGCGGGCGCCGGCGGGGGTGCAACTGACGCGAt                                                                                                          >  2:287526/1‑133 (MQ=255)
                  tGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCCCCCCACGCGGGCGCCGGCGGGGGTGAAACAGCCGCGGTCGa                                                                                                       >  5:265646/1‑135 (MQ=255)
                       aGCACGCAGCCCAGTGAATGACGGATAGAGTACAGCAAGCACAGGGTGTCCAGTGACTCGGGGCCGCGCTTGACCAGGTCTCCGAACCGCCACAGGCGGGCCCCGGCGGGGTTGAAACTGACGCGATCGAGCAg                                                                                                   >  6:141959/1‑134 (MQ=255)
                       aGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAgg                                                                                                  >  4:141032/1‑135 (MQ=255)
                           cGCAGACCAGTGAATGACGGAGCGAGTACAGGACGCGCCGGGTTTCCAGTGACGCGGGGCCGCGGTTGACCTGGGCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCAttt                                                                                             <  4:82537/136‑1 (MQ=255)
                            gCAGACCTGTTATTGCCGGTGCGACTACCGCACGCGCGGGTTGTCCTGTGACGCGGGGCCGCGGGTGCCCTGGGCCCCGACCAGCCACCGGGGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCAttt                                                                                             <  2:139138/135‑1 (MQ=255)
                            gCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGGCCACCCCCCGCCGGGCGCCCGCGGGGGTTCAACTGCCGCGATCGCGCAGGCAttt                                                                                             >  1:365535/1‑135 (MQ=255)
                                 ccAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCCCCCCCGGCGGTAGCCGGCGGGGGTGTAACTGACCCGCTAGAGCCGGCAattttcgg                                                                                        >  1:187400/1‑130 (MQ=255)
                                 ccAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCgg                                                                                       >  3:133145/1‑136 (MQ=255)
                                  cAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCCGGCGCCGGCCGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGc                                                                                      >  7:225659/1‑136 (MQ=255)
                                  cAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACACGCGGGCGACGGCGGGGGTGAAACTGACGCGCTCGCGCAGGCAATTGAGCgg                                                                                       >  5:305052/1‑135 (MQ=255)
                                      caaTGCCGGAGCGACAACAGCACGCCCCGGGTTCCCAGGGCCGCGGGGCCGCGGTGGCCCCGGCCGCCGACCAGCCCCCGGGGGCCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTgc                                                                                   <  5:186530/134‑1 (MQ=255)
                                        aggACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTgcagg                                                                                >  3:62971/3‑136 (MQ=255)
                                        aTGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGCCGGCGGGGGTGAAACTGCCGCGATCGAGCACGCATTTGAGCGGCTgccgg                                                                                >  3:221475/1‑136 (MQ=255)
                                        aTGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTgcagg                                                                                >  7:133339/1‑136 (MQ=255)
                                        aTGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCCACCCGCCACACGCGGGCGACGGCCGGGTTGAAACAGACCCCATCGAGCAGGCAATTTAGCGGCAgcagg                                                                                >  3:87552/1‑136 (MQ=255)
                                             ggATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCCCAGGCGGTCGCCGGCGGGGTTGAAACTGACGCGATCGCGCAGGCATTTGAGCGGCTGCAGGCAGc                                                                            >  2:305421/1‑135 (MQ=255)
                                             ggATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGc                                                                            >  1:147082/1‑135 (MQ=255)
                                                gcGATTACCGGACGCGCAGGGTTGCCAGGGACGGGGGGCCGCGGTTCACCTGGTCGCCGACCAGCCACAGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTg                                                                        <  3:120270/135‑1 (MQ=255)
                                                  gAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGCCGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTg                                                                        >  8:30737/1‑134 (MQ=255)
                                                  gAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCCACCAGCCACACGCGGTCGACGGCGGGGTTGAAACTGACGCGAACGAGCAGGCATTTGAGCGCCTGCACGCAGCCCAGCa                                                                      >  3:380318/1‑136 (MQ=255)
                                                    gTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCCCCCCCCCACAGGCGGTCGCCCGGGGGGTTGGAACTGACGCGCTCGAGCCGGCCTTTGAGCGGCTGCAGGCCGCCCGGccg                                                                     >  1:181135/1‑133 (MQ=255)
                                                      aCAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGt                                                                   >  4:54503/1‑135 (MQ=255)
                                                        cggACGCGCAGGGGTGCCCGGGCCGCGGGGCCGCGGTTCACCAGGGCGCCGACCAGCCACAGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGGCAc                                                                <  4:30603/135‑1 (MQ=255)
                                                        aGGACGCGCCGGGTTGCCAGGGCCGCGGGGCCGCGGTTGACCAGGCCGCCGACCCGCCACCGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCAc                                                                <  4:62971/136‑1 (MQ=255)
                                                             gcgcAGGGTTTCCAGTGACTCGGGGCCGCGGTTGCCCAGGACGCCGAACAGCCACAGGCGGTCCACGGAGGGGGTGAAACTGACGCTATAGATCAGGCATTTGAGCCGCATCACGCAGCCCTGCAGGACAccgccg                                                           >  8:389281/1‑136 (MQ=255)
                                                             gcgcAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCCCCGGCCGGCGCCGGCGGGGGTGAAACAGACGCGCTCGAGCCGGCAATTGAGCGGCTGCCGGCAGCCCTGCAGGTCACCGAc                                                            >  1:304689/1‑135 (MQ=255)
                                                                cAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCg                                                        >  4:284610/1‑136 (MQ=255)
                                                                cAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGc                                                         >  6:267508/1‑135 (MQ=255)
                                                                 aGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGCCGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCCCCGACGGCg                                                        >  3:352921/1‑135 (MQ=255)
                                                                 aGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCCGGCAGCCCTGCAGGTCACCGACGGCg                                                        >  4:273898/1‑135 (MQ=255)
                                                                     tttCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGGAgg                                                    >  2:65096/1‑135 (MQ=255)
                                                                      ttCCAGGGCCGCGGGGCCGCGGTGGACCCGGTCGCCGACAAGCCACTGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGt                                                   <  2:207373/135‑1 (MQ=255)
                                                                            tgccgcGGGGCCGGGGTTCACCCGGGCGCCGACCAGCCACAGGGGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCa                                              <  4:329838/129‑1 (MQ=255)
                                                                              acgcGGGGCCGCGGTTGACCCGGCCGCCGCCCAGCCACAGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCa                                           <  1:19876/132‑1 (MQ=255)
                                                                               gtCGGGGGCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGAGGGCGTAGGTGGCCATCAGt                                         <  7:259948/135‑1 (MQ=255)
                                                                                tCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCCCCGGCCGGCGCCGGCGGGGGTGTAACAGACGCCCTCGCGCCGGCATTTGAGCGGCTGCCGGCCCCCCTGCAGGTCACCGCCCGCGGATGGTGCCCTCAGt                                         >  2:164187/1‑135 (MQ=255)
                                                                                tCGGGGCCGCGGTTGACCAGGTCGCCGCCCCACCACACGCCGTCGACCGCGGGGTTGTAAATGACGCGAAAGAGCAGGCATTTGAGCGGCTGCAGGCAACCCTACAAGTCACCCCCCGCGTACGTTGCCAACact                                         >  2:129586/1‑133 (MQ=255)
                                                                                tCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGtt                                        >  8:203530/1‑135 (MQ=255)
                                                                                      ccGCGGTTGACCAGGTCGCCGCCCAGCCCCAGGCGGTCGACGGCGGGGGTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAgcgc                                  >  7:317700/1‑136 (MQ=255)
                                                                                            ttGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCggg                            >  4:349237/1‑136 (MQ=255)
                                                                                                 cAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGGCATCAGTGCAGCGCTCCGGGCACt                        >  6:73660/1‑135 (MQ=255)
                                                                                                  aGGTCGCCGACCAGCCACAGGCGGTCGACCGCGGGGTTGAAACTGACCCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTTCCGGTCACCCCCGGCGTAGGTGGCCAACAGTGCACCCCTCCGGGCACTg                       >  6:398748/1‑135 (MQ=255)
                                                                                                       gccgccCAGCCACAGGCGGTCGCCGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGcc                 >  7:156032/1‑136 (MQ=255)
                                                                                                       gCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGc                  >  6:110097/1‑135 (MQ=255)
                                                                                                        ccGACCAGCCACAGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCCg                <  4:306148/136‑1 (MQ=255)
                                                                                                             ccgCCCCAGGCGGGCGCCGGCGGGGGTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTACGTGGCCATCAGTGCAGCGCTCCGGGCCCTGCCAGCCCGAAg            >  6:398058/3‑135 (MQ=255)
                                                                                                              aGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCCGGAAgg           >  1:239410/1‑135 (MQ=255)
                                                                                                                       gggTGGACGGCGGGGGTGAACCTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACTGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCAGTGCCTGCCGGAAGGGGGCAAtct  <  5:141959/134‑1 (MQ=255)
                                                                                                                          |                                                                                                                                   
GCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCCGGAAGGGGGCAATCT  >  ACB122/478774‑479027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑