Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,002,324 G→T 6.6% D395E (GAC→GAA CJ019_05620 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,002,3240GT 6.6% 178.4 / 3.6 61D395E (GAC→GAACJ019_05620hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/32);  new base T (2/2);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

AGCGCGTCCATGGTCTGGCGCACCACCTCGTTGCGGCGCATCACCGCCGTCTCGGTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAAATAACCCAATAACCCAAGGGCACGATCAGGCCGCTCTGCTCG  >  ACB122/6002193‑6002459
                                                                                                                                   |                                                                                                                                       
aGCGCGTCCATGGTCTGGCGCACCACCTCGTTGCGGCGCATCACCGCCGTCTCGGTCAGCTCGAACTCAAGCCAGCGGGCCTCCCCACCGTGCGCGAGGATCAGGCGACGCAACGTGGCCAGTAGCTGGCTGTCCt                                                                                                                                     >  7:332468/1‑136 (MQ=255)
aGCGCGTCCATGGTCTGGCGCACCACCTCGTTGCGGCGCATCACCGCCGTCGCGGTCAGCGCGCACACACGCCAGCGCGCATCCCCCCCGTGCTCGAGGATCAGGCGCCGCACCGTGGCCAGTAgctgggtgggc                                                                                                                                      >  1:103729/1‑132 (MQ=255)
        cATGGTCTGGCGCACCACCTCGTTGCGGCGCATCACCGCCGTCTCGGTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTTGCTGTCCTGGAATTg                                                                                                                              <  6:152287/135‑1 (MQ=255)
          tGGTCTGGCGCACCACCTCGTTGCGGCGCATCACCGCCGTCTCGGTCAGCTCGCACTCAAGCCAGCGGGCCTCCCCACCGTGCTCGAGGATCAGGCGACACACCCTGGCCAGTAGCTGGCTGTCCTGGAACTgt                                                                                                                             >  2:378653/1‑133 (MQ=255)
                  cgcACAACCTCGTTGCGGCGCATCACCGCCGTCTCGGTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAAc                                                                                                                      >  1:6679/1‑133 (MQ=255)
                          atCGTTGCGTCGAATCACCGCCGTCTCGGTCAGCTCTAACTCAAGCCAGCGGGCATCCACACCGTGCTCTAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGACTTGGCGAAAACTCAGGTTGAcc                                                                                                           <  4:291682/135‑1 (MQ=255)
                            cGTTGCGGCGCATCACCGCCGTCTAGGTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGCCTCAACGAGGCCAGTAGCTGGCAGTCCAGGAATTGGCGAAAACTCACGTTGACCGc                                                                                                         >  4:117582/1‑136 (MQ=255)
                              ttGCGGCGCATCACCGCCGTCTCGGTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGAGAAAACTAAGGTTGACCGcc                                                                                                        <  5:36586/135‑1 (MQ=255)
                                  ggcgCATCACCGCCGTCTCGGTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCAtgtg                                                                                                   <  3:29015/136‑1 (MQ=255)
                                   gcgcATCACCGCCGTCTCGGTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCAtgtg                                                                                                   <  1:72815/135‑1 (MQ=255)
                                   gcgcAGCACCGCCGTCTCGGGCAGCGCGAACTCAAGCCCGCGGGCATCCACACCGTGCTCGAGTATCCGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCAtgtg                                                                                                   <  2:377384/135‑1 (MQ=255)
                                               cgTCTCGGTCAGCTCGATCTCAAGCCAGCGGGCATCCCCACCGTGCTCGAGGATCAGGCGACACAACGTGGCCAGTAGCTGGCTGGCCTGGAATTGGCGAAAACTCACGTTGACCGCCATGTGCAACGGtggcag                                                                                       >  2:162243/1‑130 (MQ=255)
                                                gTCTCGGTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAgg                                                                                      <  6:198417/135‑1 (MQ=255)
                                                 tctcGGTCAGATCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGcc                                                                                    <  8:222475/136‑1 (MQ=255)
                                                  ctcGGTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGcc                                                                                    <  1:323873/135‑1 (MQ=255)
                                                  ctcGGTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGcc                                                                                    <  2:6679/135‑1 (MQ=255)
                                                     ggTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCcgc                                                                                  <  4:95363/134‑1 (MQ=255)
                                                      gTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCcgc                                                                                  <  2:319779/133‑1 (MQ=255)
                                                       tCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTc                                                                              <  3:165289/136‑1 (MQ=255)
                                                       tCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTc                                                                              <  8:9026/136‑1 (MQ=255)
                                                          gCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGc                                                                            <  5:171992/135‑1 (MQ=255)
                                                            tCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGcac                                                                          >  8:161352/1‑135 (MQ=255)
                                                              gAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACaa                                                                        >  1:706/1‑135 (MQ=255)
                                                              gAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTCGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAAt                                                                       >  4:81298/1‑136 (MQ=255)
                                                                        cAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCAtgtg                                                                                                   >  4:358818/1‑98 (MQ=255)
                                                                        cAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCAtgtg                                                                                                   <  3:358818/98‑1 (MQ=255)
                                                                         aGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTc                                                            <  4:193961/136‑1 (MQ=255)
                                                                         aGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTc                                                            <  8:383684/136‑1 (MQ=255)
                                                                          gCGGTCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTc                                                            <  1:46922/135‑1 (MQ=255)
                                                                          gCGGGCATCCACACCGTGCTCGTGGATGAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTc                                                            <  1:164818/135‑1 (MQ=255)
                                                                          gCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTc                                                            <  2:42792/135‑1 (MQ=255)
                                                                                           gCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTc                                                                              >  6:82626/1‑100 (MQ=255)
                                                                                           gCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTc                                                                              <  5:82626/100‑1 (MQ=255)
                                                                                           gCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCCCAATGCCTGCATGTCACGCAGGGCACGGGaac                                           >  8:166593/1‑134 (MQ=255)
                                                                                           gCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGaa                                            >  5:10804/1‑134 (MQ=255)
                                                                                             tCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGAcc                                                                                                           >  2:97384/1‑69 (MQ=255)
                                                                                             tCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGAcc                                                                                                           <  1:97384/69‑1 (MQ=255)
                                                                                             tCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAaata                                         >  2:181451/1‑135 (MQ=255)
                                                                                             tCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAaata                                         >  5:237297/1‑135 (MQ=255)
                                                                                             tCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAaata                                         >  5:331994/1‑135 (MQ=255)
                                                                                             tCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAAAGCCTGCATGTCACGCAGGGCACGGGAaataa                                        >  7:13259/1‑136 (MQ=255)
                                                                                             tCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAAATGGCTAAAACTCATGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCCCGGGAaataa                                        >  8:81203/1‑136 (MQ=255)
                                                                                             tCGAGGATCAGGCGACTCAACGTGGCCAGAAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGAAACGGATCCAGGCAGCGCTCGCACAATGCCTGCATGTCACGCAGGTCACGTGAattaa                                        >  4:31359/1‑136 (MQ=255)
                                                                                               gAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTTTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGc                                                                     >  2:350390/1‑105 (MQ=255)
                                                                                               gAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTTTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGc                                                                     >  2:345113/1‑105 (MQ=255)
                                                                                               gAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTTTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGc                                                                     <  1:350390/105‑1 (MQ=255)
                                                                                               gAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTTTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGc                                                                     <  1:345113/105‑1 (MQ=255)
                                                                                               gAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAaataacc                                      <  7:41865/136‑1 (MQ=255)
                                                                                                agtaTCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAaataacc                                      <  6:20738/132‑1 (MQ=255)
                                                                                                   aTCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAaatacccca                                    >  1:325334/1‑134 (MQ=255)
                                                                                                     cAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAaataacccaata                                 >  1:200618/1‑135 (MQ=255)
                                                                                                       ggCGACTCAACGTTGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAaataacccaataac                               <  1:181451/135‑1 (MQ=255)
                                                                                                       ggCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAaataacccaataac                               <  8:100089/135‑1 (MQ=255)
                                                                                                       ggCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAaataacccaataac                               >  7:100089/1‑135 (MQ=255)
                                                                                                          gACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCAAGGGAAAtaacccaataacccaa                           <  4:114743/136‑1 (MQ=255)
                                                                                                                     gCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAAATAACCCAATAACCCAAGGGCACGATc                 <  8:55112/135‑1 (MQ=255)
                                                                                                                        aGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAAATAACCCAATAACCCAAGGGCACGATCAgg              <  5:333191/135‑1 (MQ=255)
                                                                                                                         gTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAAATAACCCAATAACCCAAGGGCACGATCAgg              <  7:264278/134‑1 (MQ=255)
                                                                                                                         gTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAAATAACCCAATAACCCAAGGGCACGATCAgg              >  8:264278/1‑134 (MQ=255)
                                                                                                                          tAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGcc                                                                                                        >  2:390702/1‑43 (MQ=255)
                                                                                                                          tAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGcc                                                                                                        <  1:390702/43‑1 (MQ=255)
                                                                                                                          tAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAAATAACCCAATAACCCAAGGGCACGATCAGGcc            >  1:137526/1‑135 (MQ=255)
                                                                                                                            gctggctgTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCcgc                                                                                  >  3:310218/1‑63 (MQ=255)
                                                                                                                            gctggctgTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCcgc                                                                                  <  4:310218/63‑1 (MQ=255)
                                                                                                                                   gTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAAATAACCCAATAACCCAAGGGCACGATCAGGCCGCTCTGCTCg  >  4:269107/1‑136 (MQ=255)
                                                                                                                                   |                                                                                                                                       
AGCGCGTCCATGGTCTGGCGCACCACCTCGTTGCGGCGCATCACCGCCGTCTCGGTCAGCTCGAACTCAAGCCAGCGGGCATCCACACCGTGCTCGAGGATCAGGCGACTCAACGTGGCCAGTAGCTGGCTGTCCTGGAATTGGCGAAAACTCAGGTTGACCGCCATGTGCAACGGTTCCAGGCCGCGCTCGCACAATGCCTGCATGTCACGCAGGGCACGGGAAATAACCCAATAACCCAAGGGCACGATCAGGCCGCTCTGCTCG  >  ACB122/6002193‑6002459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: