Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	6,025,859	A→G	5.8%	L126P (CTC→CCC)	CJ019_05642 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	6,025,859	0	A	G	5.8%	214.6 / 2.3	68	L126P (CTC→CCC)	CJ019_05642	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (29/35);  new base G (2/2);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCCGCCGCACCACCTCTCGCGGATAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAGCTGGCTGTACCTCACCGGGCCCAACCC  >  ACB122/6025726‑6025992                                                                                                                                      |                                                                                                                                      cgcccgccGCACCACCTCTCGCGGATAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGATACAGACCCCCGGCCACCTCGCTGGCCTCGTGCGTGAg                                                                                                                                      >  5:338884/1‑135 (MQ=255) cgcccgccGCACCACCTCTCGCGGATAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAg                                                                                                                                      <  1:144204/135‑1 (MQ=255)   cccgccGCACCCCCTCTCGCGGATAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCAt                                                                                                                                   >  8:325716/1‑136 (MQ=255)   cccgccGCACCACCTCTCGCGGATAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCAt                                                                                                                                   >  7:360996/1‑136 (MQ=255)    ccgccgCACCACCTCTCGCGGATAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATg                                                                                                                                  <  3:233965/136‑1 (MQ=255)        cgcACCACCTCTCGCGGATAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCtt                                                                                                                              >  3:81822/1‑136 (MQ=255)          caccacCTCTCGCGGATAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGGAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTc                                                                                                                            <  3:165561/136‑1 (MQ=255)           accaccTCTCTCGGATAGACACTCGCCCGATCTATCGTGCCCCTGAACATGATCTCGAATGCGAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTc                                                                                                                            <  5:316837/135‑1 (MQ=255)                     gcgGATAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTc                                                                                                                  >  5:397659/1‑135 (MQ=255)                     gcgGATAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCg                                                                                                                 <  8:360996/136‑1 (MQ=255)                      cgtaTAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCg                                                                                                                 <  5:130987/132‑1 (MQ=255)                         aTAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCAcc                                                                                                             >  7:44162/1‑136 (MQ=255)                               acgcGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGGGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGcc                                                                                                       <  3:30957/133‑1 (MQ=255)                               acTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGACTGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGcc                                                                                                       <  8:155230/136‑1 (MQ=255)                               acTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGcc                                                                                                       <  4:342562/136‑1 (MQ=255)                               acTCGCCCGATCTAGCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGcc                                                                                                       <  8:186410/136‑1 (MQ=255)                                cTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGcc                                                                                                       <  2:252238/135‑1 (MQ=255)                                cTCGCCCGATCTATCGTGCCCCTGAACAGGAGCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGcc                                                                                                       <  2:125424/135‑1 (MQ=255)                                            aTCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATg                                                                                           >  1:205067/1‑135 (MQ=255)                                             tCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGt                                                                                                <  8:229944/129‑1 (MQ=255)                                              cGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATg                                                                                           <  6:192673/133‑1 (MQ=255)                                              cGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATg                                                                                           <  1:56934/133‑1 (MQ=255)                                                     cTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGcc                                                                                                       >  8:153591/1‑114 (MQ=255)                                                     cTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGcc                                                                                                       <  7:153591/114‑1 (MQ=255)                                                     cTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTccc                                                                                 >  7:43203/1‑136 (MQ=255)                                                     cTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTcc                                                                                  >  5:170951/1‑135 (MQ=255)                                                          cAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGCCACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCt                                                                              >  1:177768/1‑134 (MQ=255)                                                          cAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCt                                                                              >  1:24946/1‑134 (MQ=255)                                                          cAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTc                                                                             <  2:175631/135‑1 (MQ=255)                                                           aGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCaa                                                                           <  3:200692/136‑1 (MQ=255)                                                                ctcGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGCACACCTCGCTGGCCTCCTGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGAc                                                                      >  3:200763/1‑136 (MQ=255)                                                                ctcGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGa                                                                       >  6:212244/1‑135 (MQ=255)                                                                ctcGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGa                                                                       >  1:349665/1‑135 (MQ=255)                                                                ctcGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGAc                                                                      >  3:369069/1‑136 (MQ=255)                                                                ctcGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTCTCCATGGCAGACTCCCGCTCAATCGAc                                                                      >  7:151775/1‑136 (MQ=255)                                                                   gactGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGATCACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCg                                                                        <  4:208855/128‑1 (MQ=255)                                                                    aaTGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTg                                                                  <  4:81822/136‑1 (MQ=255)                                                                           gTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTg                                                           <  7:325716/136‑1 (MQ=255)                                                                             ggCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGc                                                          <  1:346514/135‑1 (MQ=255)                                                                                cGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCCTGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGc                                                      <  8:228297/136‑1 (MQ=255)                                                                                cGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGc                                                      <  8:112514/136‑1 (MQ=255)                                                                                 ggggCTTCGTATCGAGAAACAGACACCCGAACACCTCGCGGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGc                                                      <  6:251605/132‑1 (MQ=255)                                                                                 ggTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGc                                                      <  2:109283/135‑1 (MQ=255)                                                                                  gTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCgg                                                    <  3:32056/136‑1 (MQ=255)                                                                                    gCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGcc                                                  >  3:386710/1‑136 (MQ=255)                                                                                            tCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCaa                                          <  3:155159/136‑1 (MQ=255)                                                                                            tCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCa                                           <  4:369069/135‑1 (MQ=255)                                                                                            tCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCa                                           <  8:363609/135‑1 (MQ=255)                                                                                             cGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCaa                                          <  6:267540/135‑1 (MQ=255)                                                                                             cGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTCTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCa                                           <  1:360123/134‑1 (MQ=255)                                                                                                      gACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTCCGGCCTATCTCAAGCAGTGCCt                                 >  1:78693/1‑135 (MQ=255)                                                                                                       acacCCTAAAACCTCGCTCGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGGCGCACCGCTGCCGTGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGTTGCCTGCGGCCTATCTAAAGCAGTGCCTg                                <  1:209552/135‑1 (MQ=255)                                                                                                             gAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAgctg                          <  2:278907/135‑1 (MQ=255)                                                                                                                 acCTCGCTGGCCTCATGCCTGGGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAgctggct                       >  5:257555/1‑134 (MQ=255)                                                                                                                 acCTCGCTGGCCTCATGCCTGGGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAgctggct                       >  5:252757/1‑134 (MQ=255)                                                                                                                 acCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAgctggct                       >  1:378250/1‑134 (MQ=255)                                                                                                                        tGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGCCTCCCGCTCACTCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAGCTGGCTGTACCTCa               >  1:47867/1‑135 (MQ=255)                                                                                                                        tGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTTCAGCTGGCTGTACCTCAc              >  4:331706/1‑136 (MQ=255)                                                                                                                        tGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAGCTGGCTGTACCTccc              >  8:328120/1‑134 (MQ=255)                                                                                                                        tGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAGCTGGCTGTACCTCa               >  1:9710/1‑135 (MQ=255)                                                                                                                        tGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAGCTGGCTGTACCTCAc              >  7:222218/1‑136 (MQ=255)                                                                                                                            cTCATGCCTGGGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTTCCTGCGGCCTATCTCAAGCAGTGCCTGCAGCTGGCTGTACCTCACCgg           <  6:252757/135‑1 (MQ=255)                                                                                                                            cTCATGCCTGGGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAGCTGGCTGTACCTCACCgg           <  6:257555/135‑1 (MQ=255)                                                                                                                                  ccTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGc                                                      >  1:138055/1‑86 (MQ=255)                                                                                                                                  ccTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGc                                                      <  2:138055/86‑1 (MQ=255)                                                                                                                                  ccTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAGCTGGCTGTACCTCACCGGGcccaa     >  2:28659/1‑135 (MQ=255)                                                                                                                                  ccTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATATCAAGCAGTGCATGCAGCTGGCTGGACCTCACCGGGccccac    >  8:252427/1‑136 (MQ=255)                                                                                                                                  ccTGACCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCAAGGCAGACTCCCGCTCAATCGACGTTGCAAGGTTCCTGCGGCCTATCTCAAGCAGTGCCTGCAGCTGGCTGTACCTCACCGGGccccc     >  6:368356/1‑133 (MQ=255)                                                                                                                                      aGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAGCTGGCTGTACCTCACCGGGcccaaccc  <  8:43203/134‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      CGCCCGCCGCACCACCTCTCGCGGATAGACACTCGCCCGATCTATCGTGCCCCTGAACAGGATCTCGAATGCCAGTGGCCGGTGCTTGGTATCGAGAAACAGACACCCGAACACCTCGCTGGCCTCATGCCTGAGCATGGCTTTCAAGTAGCGTCGCACCGCTGCCGGGCTGTCCATGGCAGACTCCCGCTCAATCGACGTTGCAAGGTGCCTGCGGCCTATCTCAAGCAGTGCCTGCAGCTGGCTGTACCTCACCGGGCCCAACCC  >  ACB122/6025726‑6025992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑