Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,038,652 A→C 27.1% L39V (TTG→GTG)  puuD_3 ← Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,038,6520AC27.1% 76.5 / 4.9 69L39V (TTG→GTG) puuD_3Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD
Reads supporting (aligned to +/- strand):  ref base A (26/20);  new base C (2/19);  total (28/41)
Fisher's exact test for biased strand distribution p-value = 3.66e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCA  >  ACB122/6038527‑6038784
                                                                                                                             |                                                                                                                                     
gggggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAg                                                                                                                            >  3:232467/1‑136 (MQ=255)
   ggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAgcc                                                                                                                          >  2:161799/1‑135 (MQ=255)
   ggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGCCAAGATCAGTGGCAAGCCGGCAAAgcag                                                                                                                         >  8:284731/1‑134 (MQ=255)
    gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAgccgg                                                                                                                        >  4:348852/1‑136 (MQ=255)
    gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAgccg                                                                                                                         >  2:141444/1‑135 (MQ=255)
          ttGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCt                                                                                                                  >  7:2383/1‑136 (MQ=255)
              aaTGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGa                                                                                                               >  8:319544/1‑135 (MQ=255)
                  atgCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCTTGCCGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCAGGCAAGATCAGTGGCACGCCCGCCAAGCCGGCCGCCGCGACAAAc                                                                                                          >  7:341271/1‑136 (MQ=255)
                     cGGCTCGATATTAGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGAGTCGCTGCCGCCATCACGCGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATAcat                                                                                                        >  5:191823/1‑133 (MQ=255)
                     cGGCGCGATTTTTGACGGCGCGCGGGAAAAAAAAATGCCGTGCCGGCGAGCCCGCAGCGCTGGCGGGGCGCGGCCGCCACCCCGGGCCGCAAAGATCAGTGCCACGCCGGAAACGCCGGCCGCCTCGACATa                                                                                                           <  2:109723/132‑1 (MQ=255)
                       gCTCTATATTTGACGGCGAGCCGGTAAAAACAAGGCCGTGCAGGCTCGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTc                                                                                                     <  4:118198/136‑1 (MQ=255)
                       gCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGCCATACTTGTc                                                                                                     >  8:151953/1‑136 (MQ=255)
                       gCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTc                                                                                                     >  4:196693/1‑136 (MQ=255)
                       gCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGAGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACAAACTTGTc                                                                                                     >  8:345331/1‑136 (MQ=255)
                        cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCg                                                                                                    >  3:273300/1‑136 (MQ=255)
                          cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGcc                                                                                                  >  3:118198/1‑136 (MQ=255)
                          cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGc                                                                                                   >  1:380325/1‑135 (MQ=255)
                           gATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGcc                                                                                                  >  6:93777/1‑135 (MQ=255)
                                   acggCGCGCGGGTAAAAACATTGCCGGGCTGGCTAGCCCGCAGCGCTGGCGTGCCGCTGCCGCCATCAGGGGCCGGCAAGATCAGTGCAACGCCGCAAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCgggt                                                                                          <  8:314080/133‑1 (MQ=255)
                                    cggCGTGCCGTTAAAAACAATGCGGGGCCGGCGAGCCAGCAGCGCTGGCGTGGCGCGGCCGCCATCACGGGCCGGCAAGATCTGGGCCAAGCGGGAAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGc                                                                                        <  3:131117/135‑1 (MQ=255)
                                     ggCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGc                                                                                        >  7:294610/1‑135 (MQ=255)
                                          gCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGCCATACTTGTCGCCGACCGTGTGCgagga                                                                                   >  1:344305/1‑135 (MQ=255)
                                             ggaaaaaaCAATGCCGGGCTGGCGAGCCCGCAGCGCTGGCGTGGCGCGGCCGCCATCCCGGGCCGGCAAGACCCGGGGCACGCCGGAAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGttc                                                                               <  4:61245/133‑1 (MQ=255)
                                                 aaaaCAATGCCGTGCAGGCGCGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGttcttac                                                                           >  8:196107/1‑134 (MQ=255)
                                                           cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGCCATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACctgct                                                                  >  1:379106/1‑135 (MQ=255)
                                                           cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACctgc                                                                   >  1:337399/1‑134 (MQ=255)
                                                            gTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGa                                                                >  3:72089/1‑136 (MQ=255)
                                                             tGCCGGCGACCCTGCTGCGGTTGGGTGGCGCTGCCGCCATCCGGGGCCGGAAATATCCGGGGCACGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGa                                                                <  2:36022/135‑1 (MQ=255)
                                                                aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCCAGCCGGCAAAGCCGGCCGCCTCGACAGACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACgg                                                             >  3:294470/1‑135 (MQ=255)
                                                                aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCACGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGGGGGCGCGGAGGTCTTCCCTACCTGCAGACgg                                                             >  2:288104/1‑135 (MQ=255)
                                                                aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACgg                                                             >  3:181506/1‑135 (MQ=255)
                                                                          gcagcGCTTGGGGGGCGCTGCCGCATTCAGGGGCCGGAAAGATCAGGGCCACGCGGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa                                                   <  2:5848/135‑1 (MQ=255)
                                                                          gacgcgcTGGCGGGTCGCGGCCGCCAGCCCGGGCGGACAAGATCTGTGCCAACCTGGAAATGGGGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa                                                   <  2:16148/132‑1 (MQ=255)
                                                                                                    tcccGGGCCGGCAAGAGCAGGGGCACGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  3:190992/133‑1 (MQ=255)
                                                                                                    tcccGGGCCGGAAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  7:64292/133‑1 (MQ=255)
                                                                                                    tcccGGGCCGCCAAGATCAGTCGAACCCGGGAAACGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  3:348852/133‑1 (MQ=255)
                                                                                                    tCAGGGGCGGGCATGATCAGTGGCATGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  4:185164/136‑1 (MQ=255)
                                                                                                    tCACGGGCGGGAAAGATCAGTGGCACGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  4:99520/136‑1 (MQ=255)
                                                                                                    tCACGGGCGGCAAAGATCAGTGGCAACCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTTCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  4:387694/136‑1 (MQ=255)
                                                                                                    tCACGGGCCGGCAAGTTCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  4:99519/136‑1 (MQ=255)
                                                                                                    tCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  4:273300/136‑1 (MQ=255)
                                                                                                    tCACGGGCCGGCAAGATCAGGGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  7:319544/136‑1 (MQ=255)
                                                                                                    tCACGGGCCGGAAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  7:69631/136‑1 (MQ=255)
                                                                                                    gcccGGGCCGGCAAGATCCGGGGCAAGCCGCCAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  4:232467/133‑1 (MQ=255)
                                                                                                    gcccGGGCCGGCAAGATCAGTGGCAACCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  3:316679/133‑1 (MQ=255)
                                                                                                    cccggggCGGGAAATAGCAGGGGCACGCCGGCAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  8:294610/132‑1 (MQ=255)
                                                                                                    cccggggCCGGCAAGATCTGGGCCACGCCGCAAACGCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  3:48298/132‑1 (MQ=255)
                                                                                                    ccccgggcgggCAATACCTGGGCCACGCCGGAAAAGCGGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  8:169812/133‑1 (MQ=255)
                                                                                                     ccggggcgggcAAGATCAGTGGCACGCCGGAAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  2:313345/132‑1 (MQ=255)
                                                                                                     ccggggCCGGCATGTGCCGGGGCACGCGGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  1:77397/132‑1 (MQ=255)
                                                                                                     ccggggCCGCCAATAGCTGGGCCACGCCGCAAAAGCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  1:278997/132‑1 (MQ=255)
                                                                                                     cccGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  2:76986/133‑1 (MQ=255)
                                                                                                     cccGGGCCGCCAATATCCGGGGAACGCCGGAAACGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  5:272737/133‑1 (MQ=255)
                                                                                                     cACGGGCGGGCAAGATCCGGGCCACGCCGGAAACGCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  2:280646/135‑1 (MQ=255)
                                                                                                     cACGGGCCGGCAAGATCAGTGGCAAGCCGGAAAAGCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  6:109533/135‑1 (MQ=255)
                                                                                                     cACGGGCCGGAAAGACCAGTGCCACCCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  6:314150/135‑1 (MQ=255)
                                                                                                     cACGGGCCGCAAAGATCAGTGCCAAGCGGGAAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCTAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  2:113109/135‑1 (MQ=255)
                                                                                                      tggggCCGGCTAGTGCTGTGGCAAGGGGGATAAGCGGGCCGCCTCGACATACTTGTCGCCGAGCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGTTCTAAGGGACCGCATTTgcgc                        <  6:191823/132‑1 (MQ=255)
                                                                                                      gggggCGGGAAAGATGTGGGGCAGCCGGCAAAGGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  1:141444/132‑1 (MQ=255)
                                                                                                      aggggCCGCCAAGCCCCGGGCCACGCCGGAATTGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTc                      <  7:283289/134‑1 (MQ=255)
                                                                                                              ggtataTCTGTGGCTTGGCGGCAAAGCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCTTTTGCGCTCATGGGCtt              <  7:284731/131‑1 (MQ=255)
                                                                                                              gCAAGATCAGTGGCACGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt              <  4:344745/136‑1 (MQ=255)
                                                                                                              gCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt              <  8:198373/136‑1 (MQ=255)
                                                                                                              gCAAGATCAGTGCCACGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt              <  4:31740/136‑1 (MQ=255)
                                                                                                                aaGATCAGTGGCAAGCCGGCAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTaca           <  4:355362/136‑1 (MQ=255)
                                                                                                                       gTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGCCACCGATCAAAGGGCCCGCATTTGCGCTCATGGGCTTACACCgtg      >  5:315126/1‑135 (MQ=255)
                                                                                                                           cAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGCCGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGGGCa  >  2:133587/1‑135 (MQ=255)
                                                                                                                           cAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        >  3:289158/1‑113 (MQ=255)
                                                                                                                           cAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                        <  4:289158/113‑1 (MQ=255)
                                                                                                                             |                                                                                                                                     
GGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCA  >  ACB122/6038527‑6038784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: