Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,102,900 G→A 6.7% L21L (CTG→TTG)  CJ019_05717 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,102,9000GA 6.7% 191.1 / 2.1 59L21L (CTG→TTG) CJ019_05717hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/26);  new base A (2/2);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCCATGTGACCCAAGCCCATT  >  ACB122/6102767‑6103032
                                                                                                                                     |                                                                                                                                    
ttCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGc                                                                                                                                     >  1:66081/1‑135 (MQ=255)
 tCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCAc                                                                                                                                   >  8:333287/1‑136 (MQ=255)
 tCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAACa                                                                                                                                    >  2:146366/1‑135 (MQ=255)
      tcaatcaCCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAg                                                                                                                              >  3:59926/1‑136 (MQ=255)
          tcaCCCGCTGATCAGCCACGGGCGGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCACGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCg                                                                                                                           <  5:390976/135‑1 (MQ=255)
            aCCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGtc                                                                                                                         >  6:133641/1‑135 (MQ=255)
                gCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGcc                                                                                                                    <  4:72516/136‑1 (MQ=255)
                 cTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGcc                                                                                                                    <  5:96078/135‑1 (MQ=255)
                 cTGAGCAGACAAGTGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCACGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGcc                                                                                                                    <  6:15834/135‑1 (MQ=255)
                       aGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGTCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACAcc                                                                                                             <  7:82263/136‑1 (MQ=255)
                       aGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACAcc                                                                                                             <  7:273602/136‑1 (MQ=255)
                       aGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACAcc                                                                                                             <  7:125416/136‑1 (MQ=255)
                       aGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACAcc                                                                                                             <  3:39120/136‑1 (MQ=255)
                           aaGGGCTGGCCAAGGCTGATCCCCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCCGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAACCACCTg                                                                                                           >  1:88652/1‑134 (MQ=255)
                                 tGGCCAAGGCTGATCACCATTGCGGCAAAGTCCTCCAGGTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGatat                                                                                                   <  3:159949/136‑1 (MQ=255)
                                 gggacAAGGATTATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGTCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGatat                                                                                                   <  7:246769/132‑1 (MQ=255)
                                      aaGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCg                                                                                                                      >  3:138533/1‑112 (MQ=255)
                                      aaGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCg                                                                                                                      <  4:138533/112‑1 (MQ=255)
                                      aaGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCca                                                                                                >  7:10707/1‑134 (MQ=255)
                                                aCCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGa                                                                                     >  2:209677/1‑135 (MQ=255)
                                                aCCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGa                                                                                     >  6:318792/1‑135 (MQ=255)
                                                aCCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAg                                                                                    >  3:173593/1‑136 (MQ=255)
                                                       cGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGtt                                                                             >  8:155300/1‑136 (MQ=255)
                                                       cGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGt                                                                              >  5:129329/1‑135 (MQ=255)
                                                          cAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGcc                                                                                                                    <  7:20789/94‑1 (MQ=255)
                                                          cAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGcc                                                                                                                    >  8:20789/1‑94 (MQ=255)
                                                                    cAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCt                                                                 <  4:333288/135‑1 (MQ=255)
                                                                     aGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTc                                                               <  8:10707/136‑1 (MQ=255)
                                                                     aGTGGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTc                                                               <  4:48917/136‑1 (MQ=255)
                                                                       ttGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCt                                                             >  8:344876/1‑136 (MQ=255)
                                                                          tgCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCAt                                                          <  3:386555/136‑1 (MQ=255)
                                                                            ccGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATg                                                         >  5:93645/1‑135 (MQ=255)
                                                                            ccGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGc                                                        >  4:56324/1‑136 (MQ=255)
                                                                            ccGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGc                                                        >  3:58872/1‑136 (MQ=255)
                                                                                       cgccgccAGACGGCCCGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGCGGCGTCTCGCCAAACACCGGAAAGATATCCACAGGTCGGGGCGCCCGGGTCCTGCTGCCGCTTCCTCATGCCCTGGGCCaa                                              >  5:377041/1‑135 (MQ=255)
                                                                                         ccgccAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTGCATGCTGCCGCTTCCTCATGCCATGGGCCAAATg                                           <  4:59926/136‑1 (MQ=255)
                                                                                            ccAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAACACTTGAGGCGTCTCGcc                                                                                                                    <  1:347990/60‑1 (MQ=255)
                                                                                            ccAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAACACTTGAGGCGTCTCGcc                                                                                                                    >  2:347990/1‑60 (MQ=255)
                                                                                                       ggTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAACCGc                             >  4:258447/1‑136 (MQ=255)
                                                                                                        gTTCACGCCATCCTGCAGCACTTCGGCCAACACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGc                             <  1:146366/135‑1 (MQ=255)
                                                                                                               ccATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCagccagcc                     <  7:325944/136‑1 (MQ=255)
                                                                                                                cATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCCTGGGCCAAATGGGGCAGGTAAACGcagccagc                      >  5:355858/1‑134 (MQ=255)
                                                                                                                cATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCagccagcc                     <  1:209677/135‑1 (MQ=255)
                                                                                                                cATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGccc                    <  3:56324/136‑1 (MQ=255)
                                                                                                                 aTCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGACAGGTAAACGCagcccgcc                     >  1:374441/1‑134 (MQ=255)
                                                                                                                      gcagcaCTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGcagcc                         >  5:227824/1‑125 (MQ=255)
                                                                                                                      gcagcaCTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGcagcc                         <  6:227824/125‑1 (MQ=255)
                                                                                                                       cagcaCTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCCATGTGa              <  4:173593/135‑1 (MQ=255)
                                                                                                                         gcaCTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCCATGTGAccc           <  7:333287/136‑1 (MQ=255)
                                                                                                                         gcaCTTCGGCCAGCAC‑‑GAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCCATGTGACCCaa         <  3:159196/136‑1 (MQ=255)
                                                                                                                          caCTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATTGGTCAGGTAAACGCAGCCAGCCCATGTGAccc           >  1:187311/1‑135 (MQ=255)
                                                                                                                          caCTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCCAGGTGCCCCa          >  3:203484/1‑136 (MQ=255)
                                                                                                                          caCTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCAATGTGAccc           >  4:79685/1‑135 (MQ=255)
                                                                                                                            cTTCGGCCAGCACTTGAGGCGGCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCCATGTGACCCaa         <  6:292987/135‑1 (MQ=255)
                                                                                                                              gcGGCCAGCTCTTGAGGCGTCTCGCCAAACACCTGAAAGATAGCCACAGGTCTGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCCATGTGACCCaa         <  2:88652/132‑1 (MQ=255)
                                                                                                                               cGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCCATGTGACCCAAGcc      >  3:343956/1‑135 (MQ=255)
                                                                                                                               cGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCCATGTGACCCAAGcc      >  1:335642/1‑135 (MQ=255)
                                                                                                                               cGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATACCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCCATGTGACCCAAGcc      >  5:24275/1‑135 (MQ=255)
                                                                                                                                  ccAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCCATGTGACCCAAGCCCAtt  >  8:192269/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                    
TTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTCCAGTTGTGCCGGCAAGCGCCGCCGCCAGACGGCCAGGTTCACGCCATCCTGCAGCACTTCGGCCAGCACTTGAGGCGTCTCGCCAAACACCTGAAAGATATCCACAGGTCGGTGAGCCAGGTTCATGCTGCCGCTTCCTCATGCCATGGGCCAAATGGGTCAGGTAAACGCAGCCAGCCCATGTGACCCAAGCCCATT  >  ACB122/6102767‑6103032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑