Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 486,274 C→A 6.7% P225Q (CCG→CAG)  amgK → N‑acetylmuramate/N‑acetylglucosamine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122486,2740CA 6.7% 166.4 / 4.6 60P225Q (CCG→CAG) amgKN‑acetylmuramate/N‑acetylglucosamine kinase
Reads supporting (aligned to +/- strand):  ref base C (40/16);  new base A (2/2);  total (42/18)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01

GCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGT  >  ACB122/486139‑486402
                                                                                                                                       |                                                                                                                                
gcgTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGccc                                                                                                                                  <  7:269788/136‑1 (MQ=255)
  gTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCgg                                                                                                                                >  4:24476/1‑136 (MQ=255)
    cAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCgg                                                                                                                                >  2:142163/1‑134 (MQ=255)
        cAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGGACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCACCCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCAcc                                                                                                                           >  7:201439/1‑135 (MQ=255)
                   cGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTAcc                                                                                                               <  7:329756/136‑1 (MQ=255)
                     aCAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTAcc                                                                                                               <  5:291320/134‑1 (MQ=255)
                            ccTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTAcc                                                                                                               <  2:201386/127‑1 (MQ=255)
                            ccTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTAcc                                                                                                               >  1:201386/1‑127 (MQ=255)
                                                   tACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGcc                                                                               >  8:271685/1‑136 (MQ=255)
                                                   tACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGc                                                                                >  1:379064/1‑135 (MQ=255)
                                                   tACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGc                                                                                >  2:34373/1‑135 (MQ=255)
                                                          tGACTACATGCCGCGCAACCTGATGCAGAGCCCCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCCCCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGgc                                                                         >  1:247958/1‑135 (MQ=255)
                                                          tGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTTTACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAg                                                                           >  4:16777/1‑133 (MQ=255)
                                                          tGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTTTACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAg                                                                           <  3:16777/133‑1 (MQ=255)
                                                          tGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCGCCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTCGCCGCAGgc                                                                         >  1:153045/1‑135 (MQ=255)
                                                          tGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTa                                                                                                                         <  5:213288/87‑1 (MQ=255)
                                                          tGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTa                                                                                                                         >  6:213288/1‑87 (MQ=255)
                                                          tGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGgcg                                                                        >  4:385494/1‑136 (MQ=255)
                                                          tGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGgc                                                                         >  1:100107/1‑135 (MQ=255)
                                                          tGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGgc                                                                         >  5:341065/1‑135 (MQ=255)
                                                          tGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGgc                                                                         >  6:270463/1‑135 (MQ=255)
                                                           gACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGgcgc                                                                       >  3:197103/1‑136 (MQ=255)
                                                            aCTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGcgcg                                                                      <  8:84741/136‑1 (MQ=255)
                                                            aCTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGcgcg                                                                      <  7:332148/136‑1 (MQ=255)
                                                             cTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTcc                                                                                                                             >  2:225927/1‑79 (MQ=255)
                                                             cTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCa                                                                                                                             <  1:225927/80‑1 (MQ=255)
                                                             cTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAg                                                                           >  2:86951/1‑130 (MQ=255)
                                                             cTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAg                                                                           <  1:86951/130‑1 (MQ=255)
                                                             cTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGcgcg                                                                      >  6:274884/1‑135 (MQ=255)
                                                               aCATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGt                                                                   >  4:355664/1‑136 (MQ=255)
                                                                   gCCGCGCAACCTGAAGCAGAGCACCCCCAACCCTGGTGTGCTGCACTTTCTGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCATGTTCAAGGACGCCTTAGACAGCTGGCCGCAGGCGCCGGTc                                                                  >  5:355511/1‑133 (MQ=255)
                                                                        gcACCCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGCCTTTCAGGATGCGGTATACGGCCCGGTCACCTACGCCTTTACCTGCCTGTTCAAGGCCGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTg                                                          >  4:52139/1‑136 (MQ=255)
                                                                         cAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTg                                                          >  5:190641/1‑135 (MQ=255)
                                                                          aaCCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTAAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTggg                                                         >  2:185699/1‑135 (MQ=255)
                                                                                         aCCCCCCACCCTGGTGTGCTGGACTTTCAGGCTGCGGTATCCGGCCCGGTCACCTACGACATTCCCTGGCTGTTCAAGGACGCCTTTGTCTGCGGGCCGCAGGCGGGGGTCGATGGTTGGCTGGGCGGCGACTgg                                          >  2:116962/1‑135 (MQ=255)
                                                                                         aCCCCCACCCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCATTTTCAGCTGGCCGCAGGCGCGGGTCGATGGTTGGCTGGGCGACTACTgg                                          >  5:219296/1‑135 (MQ=255)
                                                                                         aCCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGTCATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTg                                           >  5:285564/1‑134 (MQ=255)
                                                                                         aCCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTgg                                          >  2:169308/1‑135 (MQ=255)
                                                                                         aCCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTgg                                          >  3:355793/1‑135 (MQ=255)
                                                                                         aCCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTgg                                          >  6:5123/1‑135 (MQ=255)
                                                                                         aCCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCAGGTCACCTACGACATTAcc                                                                                                               <  4:226719/66‑1 (MQ=255)
                                                                                         aCCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCAGGTCACCTACGACATTAcc                                                                                                               >  3:226719/1‑66 (MQ=255)
                                                                                         aCCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCAGGTCACCTACGACATTAcc                                                                                                               >  3:222076/1‑66 (MQ=255)
                                                                                         aCCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCAGGTCACCTACGACATTAcc                                                                                                               <  4:222076/66‑1 (MQ=255)
                                                                                         aCCCCCAACCCTGGTGTGCTGGACTTTCAGGACGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGact                                               <  4:239637/130‑1 (MQ=255)
                                                                                         aCCCCCAACCCTGGTGTGCTGGACTTTCAGGAAGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGact                                               >  3:239637/1‑130 (MQ=255)
                                                                                             ccAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGgca                                        >  5:139374/1‑133 (MQ=255)
                                                                                              cAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGgcagca                                     >  5:85060/1‑135 (MQ=255)
                                                                                                   cTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAgcaggc                                  >  5:93178/1‑133 (MQ=255)
                                                                                                        gtgCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAggcgg                          >  7:290397/1‑136 (MQ=255)
                                                                                                        gtgCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAggcgg                          >  7:303265/1‑136 (MQ=255)
                                                                                                            tGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTg                       >  5:365994/1‑135 (MQ=255)
                                                                                                               aCTTTCAGGATGCGGTATACGGGCCGGTCCCCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCa                    >  1:155020/1‑135 (MQ=255)
                                                                                                                 tttCAGGATGCGGTATACGGCCCGGTCACCTACGACATTAcc                                                                                                               >  5:67272/1‑42 (MQ=255)
                                                                                                                 tttCAGGATGCGGTATACGGCCCGGTCACCTACGACATTAcc                                                                                                               <  6:67272/42‑1 (MQ=255)
                                                                                                                     aGGATGCGGTATACGGCCCGGTCGCCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCg               <  2:153045/134‑1 (MQ=255)
                                                                                                                           cGGTATACGGCCCGGTCACCTACGACATAACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGTATCCCGGTGCAc         <  1:142163/134‑1 (MQ=255)
                                                                                                                                taCGGCCCGGTCACCTACGACATTACCTGCCTGTTCACGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGt  <  3:98025/136‑1 (MQ=255)
                                                                                                                                taCGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGt  <  3:24476/136‑1 (MQ=255)
                                                                                                                                taCGGCCCGGTCACCTACGACATTACCTGCATGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGt  >  7:265201/1‑136 (MQ=255)
                                                                                                                                 aCGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGt  <  5:274884/135‑1 (MQ=255)
                                                                                                                                      ccGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGgc                                         <  6:277533/91‑1 (MQ=255)
                                                                                                                                      ccGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGgc                                         >  5:277533/1‑91 (MQ=255)
                                                                                                                                       |                                                                                                                                
GCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCTGTTCAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGT  >  ACB122/486139‑486402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑