| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 539,413 | C→T | 6.4% | S64S (TCC→TCT) ‡ | rpsL → | 30S ribosomal protein S12 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 539,413 | 0 | C | T | 6.4% | 201.9 / 2.8 | 62 | S64S (TCC→TCT) ‡ | rpsL | 30S ribosomal protein S12 |
| Reads supporting (aligned to +/- strand): ref base C (23/35); new base T (2/2); total (25/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGTTCCTGCGCTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGTTAAAGG > ACB122/539280‑539547 | aCGTTCCTGCGCTGCAGAACTGCCCGCAGCGTCGTTGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTc < 8:207442/136‑1 (MQ=255) cGTTCCTGCGCTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTc < 2:77892/135‑1 (MQ=255) ttCCTGCGCTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTa < 4:91264/136‑1 (MQ=255) cTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCggtg < 4:335299/136‑1 (MQ=255) cTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCggtg < 4:340926/136‑1 (MQ=255) cTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCggtg < 4:360730/136‑1 (MQ=255) aCTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg < 7:280742/136‑1 (MQ=255) cTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg < 2:314443/135‑1 (MQ=255) cccGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCCCGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGGGAAGGCcac > 7:312506/1‑136 (MQ=255) cccGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCggtg > 8:227740/1‑125 (MQ=255) cccGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCggtg < 7:227740/125‑1 (MQ=255) ccGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGcc < 1:114690/133‑1 (MQ=255) gCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACaa > 6:392215/1‑135 (MQ=255) cgtcgtGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTg < 1:97115/135‑1 (MQ=255) cgtcgtGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTg < 2:360512/135‑1 (MQ=255) tGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAaga < 5:351571/135‑1 (MQ=255) tGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAaga < 5:392215/135‑1 (MQ=255) ggCGTGTGCACCCGTGTGTACACCACCACGCCGCAAAAACCAAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGCCCAACGGTTTAGAGGTTTCCTCGTACATCGGTGGTGCAGGCCCCCACCTGCAagag > 4:374922/1‑135 (MQ=255) cGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTACTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGca > 8:68097/1‑135 (MQ=255) gtgtGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGcaca < 7:390272/136‑1 (MQ=255) ccccGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGGTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGcgtc > 1:13096/2‑135 (MQ=255) aCCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGcgtcg > 3:202670/1‑136 (MQ=255) gtgtACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTg < 2:191166/135‑1 (MQ=255) gtgtACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTg < 1:98153/135‑1 (MQ=255) gtACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg > 6:81520/1‑102 (MQ=255) gtACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg < 5:81520/102‑1 (MQ=255) gtACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg < 5:75696/102‑1 (MQ=255) gtACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg > 6:75696/1‑102 (MQ=255) caccacGCCTAATAAAGCTATCTCGGCACTGCGTAAAGTTTGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCTGTGGTGAAGGCCACAACCTTCAAGAGCACAGCGTCGTCCTGATTCGTgg < 6:131098/135‑1 (MQ=255) caccacGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTgg < 2:13096/135‑1 (MQ=255) accacGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTggcg < 7:56349/136‑1 (MQ=255) accacGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTggc > 1:60564/1‑135 (MQ=255) cacTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCTGACTAACGGTTTCGAAGTGACTTCCTACATCGGTGGTGAAGGTCACAACCTGCAgga < 1:18966/108‑1 (MQ=255) cacTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCTGACTAACGGTTTCGAAGTGACTTCCTACATCGGTGGTGAAGGTCACAACCTGCAgga > 2:18966/1‑108 (MQ=255) cacGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTcc > 5:242973/1‑73 (MQ=255) cacGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTcc < 6:242973/73‑1 (MQ=255) gCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCgt > 7:334628/1‑136 (MQ=255) gCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCg > 6:343266/1‑135 (MQ=255) cGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTGGAGGTTTCCTCGTa < 5:244761/73‑1 (MQ=255) cGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTa > 6:244761/1‑73 (MQ=255) cGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCgt > 2:240521/1‑134 (MQ=255) aaaaaCCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCTGACTAACGGTTTCGAAGTGACTTCCTACATCGGTGGTGAAGGTCACAACCTGCAGGAGCACTCCGTGATCCTGATCCGTGGCGGTCgtgtt > 6:9047/1‑133 (MQ=255) aaaaCCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTaaa < 5:269369/135‑1 (MQ=255) aaaaCCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTaaa < 5:343266/135‑1 (MQ=255) aaaCCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGAATCGTGGCGGCCGTGTaa > 1:18906/1‑133 (MQ=255) tAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGACACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACtt < 5:80678/135‑1 (MQ=255) ggCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGgt < 3:235240/136‑1 (MQ=255) ggCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGgt < 3:262613/136‑1 (MQ=255) aaaGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg > 3:230407/1‑62 (MQ=255) aaaGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg < 4:230407/62‑1 (MQ=255) aaaGAATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACTTCGTTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTTCTTGCGCGCGTGTAAAAGTCTTGCACGGTGttggggtc > 1:168216/1‑129 (MQ=255) aTGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACAcc > 8:55613/1‑136 (MQ=255) gtgtTCGTCTGACTAACGGTTTCGAAGTGACTTCCTACATCGGTGGTGAAGGTCACAACCTGCAGGAGCACTCCGTGATCCTGATCCGTGGCGGTCGTGTTAAAGACCTCCCGGGTGTTCGTTACCACACCGtac < 5:9047/135‑3 (MQ=255) gtgtGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTc < 1:240521/135‑1 (MQ=255) cGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTCCCACACCGTTCGCGGc > 6:272390/1‑135 (MQ=255) cGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGct > 4:348209/1‑136 (MQ=255) gTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTACTTATTCGTGGAGGCCGTGTAAAATACTTGCAAGGTGGTCGTTACCACACCGTTCGCGGc < 2:26476/134‑1 (MQ=255) tCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGctct > 3:385594/1‑136 (MQ=255) aCGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATAc < 4:202670/136‑1 (MQ=255) tttCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTgg < 2:205223/72‑1 (MQ=255) tttCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTgg > 1:205223/1‑72 (MQ=255) tCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCgg < 8:96719/135‑1 (MQ=255) aGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTTAAAGACTTGCCAGGTGTTCGTTAACACACCGTTAGCGGGTCTCTGTATACTTCgggggg > 7:240396/1‑133 (MQ=255) aGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGt < 8:351326/136‑1 (MQ=255) ccTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGTTAAAg < 4:38675/135‑1 (MQ=255) ccTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGTTAAAg < 3:348209/135‑1 (MQ=255) ccTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGGGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGTTAAAg < 4:54822/135‑1 (MQ=255) cTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGTTAAAgg < 7:68097/135‑1 (MQ=255) | ACGTTCCTGCGCTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGTTAAAGG > ACB122/539280‑539547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |