Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	797,531	A→C	28.5%	intergenic (+77/+17)	CJ019_00762 → / ← CJ019_00763	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	797,531	0	A	C	28.5%	67.8 / 7.3	45	intergenic (+77/+17)	CJ019_00762/CJ019_00763	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (29/2);  new base C (3/11);  total (32/13)
Fisher's exact test for biased strand distribution p-value = 2.36e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTGCCACCTTC  >  ACB122/797408‑797664                                                                                                                            |                                                                                                                                      cGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAACGGGCCGCAACGCGGCCCCgg                                                                                                                            >  6:290422/1‑135 (MQ=255)  gCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCTGTGGTTGGACACAGGCCGTCGTAGGAGCGGGGTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCt                                                                                                                          >  3:97527/1‑136 (MQ=255)  gCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAACGGGCCGCCACGCGGCCCCgg                                                                                                                            >  5:202000/1‑134 (MQ=255)  gCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGc                                                                                                                           >  1:136390/1‑135 (MQ=255)  gCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGc                                                                                                                           >  4:68524/1‑135 (MQ=255)  gCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGc                                                                                                                           >  4:110956/1‑135 (MQ=255)  gCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGc                                                                                                                           >  3:291597/1‑135 (MQ=255)          cgtggccTGCAATTCCGATCCTGCCGGGGGCGAATTGATAGCCCGCTCCGCCAAACTCATGGGGGGGTGGCCACCGGCCGGCGTCGGCGGGGGTCCCCCCGCGAACGGGCCGCACCGGGGCCCCGGCTATCTCAgg                                                                                                                  <  8:112598/130‑1 (MQ=255)                cggCCATTCCCAACGGGCCTGGGGCTCTTTTATCCCCCGCGCCTGAATCCGCATGGGTGGTTGCACACTGGCCGTCGTAGGCGCGGGTCCCCCCGCTAACGGGCCGCACCGGGGCCCCGGCTATCTCAGGCCAGtt                                                                                                            <  8:79707/134‑1 (MQ=255)                            tCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCTGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGAt                                                                                                >  7:77404/1‑136 (MQ=255)                              cTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATcc                                                                                              >  4:136042/1‑136 (MQ=255)                                    tGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGAGGTTGCACACAGGCCGTCGTAGGAGCGGGTTCACCCCCGAAAGGGCCGCCACGCGGCCCCCGCTAACTAACGCCAGTTCCACGGCCAGCACCACACGc                                                                                        >  3:249120/1‑136 (MQ=25)                                      ggctatttGATCGCCCGCGCCTGCAAACGAAGGGGTGGTTGCACACCGGCCGGCGTAGGAGCGGGTGCACCCGCTAACGGGCCGCACCGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGcac                                                                                      <  8:81188/130‑1 (MQ=255)                                              gATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCCACGCCGCCCCCGCGATATCACGCCCGGTGCGCGGCCGGAGGCGCACGCCCCAcccggg                                                                              >  7:149144/1‑132 (MQ=16)                                              gATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCACAGGCCAGTTCCGAGGCCTGATACTCACGCACAACCGCtt                                                                              >  7:34967/1‑136 (MQ=255)                                               aTAGCCCGCTCCTGCATACGCATGGGGGGTTGGACACCGGCCGTCGTAGGCGCGGGTTCACCCGCTAACGGGCCGCACCGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCtt                                                                              <  3:110956/135‑1 (MQ=38)                                                      gCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAACGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGGTCCGCGGCCTGCTCCTCACGCCCCACCGCGTTCAcgt                                                                        >  5:377206/1‑132 (MQ=25)                                                        tCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCCCACCGCGGCCCCGGCTCTCGCAGGCCCGGTCCTCGGGCTGATCCTCCCGCCCAACCGCGTTCACGTCag                                                                     >  2:347920/1‑134 (MQ=21)                                                          cTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAACGGGCCGCAACGCGGCCCCGGCTATATAAGGCCAGTTCCGCGGCCTGATCCTCACCCACAACCCCGTTCACTTCATcc                                                                   >  2:117872/1‑135 (MQ=37)                                                          cTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGGCTGATCCTCACGCACACCCGCGTTCACTTCATcc                                                                   >  5:102022/1‑135 (MQ=255)                                                          cTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGCTCCTCACGCCCAACCGCTTTCACTTCATccc                                                                  >  4:387617/1‑135 (MQ=255)                                                          cTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCCCGCACAACCGCGTTCACATCATcc                                                                   >  2:337487/1‑135 (MQ=255)                                                          cTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCGGCTCCTCACGCACAACCGCTTTCACTTCATccc                                                                  >  8:370666/1‑135 (MQ=255)                                                          cTGCATACGCATCGGTGGTTGGACACAGGACGTCGTAGGAGCGGGTTCACCCGCGAAACGGCCGCACCCCCGCCCCCGCTATCTCAGGCCCCTTCCTCGCCCTGATCCTCACGACCCACCGCGTTCACATCATCCa                                                                  >  4:345609/1‑136 (MQ=21)                                                                  gcaggggtggtTGCACACAGGCCGGCGTAGGAGCGGGTTCACCCCCCATCGGGCCCCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCtg                                                          <  7:316545/131‑1 (MQ=38)                                                                  gCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACACCCGCTTTCACTTCATCCAGCCGGCtg                                                          >  8:355561/1‑136 (MQ=255)                                                                  gCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCt                                                           >  4:248501/1‑135 (MQ=255)                                                                     tCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCtgatg                                                       >  4:55450/1‑136 (MQ=255)                                                                     tCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCtga                                                         >  2:313260/1‑134 (MQ=255)                                                                       ggtggtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCACCGCGGCCCCGGCTATATCAGGCCAGGTCCTCGGGCTGATCCTCACGCCCACCCGCGTTGACTTAATCCCGCCGGCtgatga                                                      >  1:217618/1‑135 (MQ=25)                                                                       ggtggtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAACGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGCTCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGaa                                                     >  8:195151/1‑136 (MQ=255)                                                                         tggtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACCACCGCTTTCACTTCAACCAGCCGGCTgaagaaat                                                   >  3:77335/1‑134 (MQ=255)                                                                         tggtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAAc                                                    >  6:203857/1‑135 (MQ=255)                                                                                     ggCCGTCTTCGGTGGGGGTTCACCCGCTAATGGGCCGCACCGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCt                                       <  4:140580/136‑1 (MQ=255)                                                                                      gCCGGCGTTGGCGCGGGTCCCCCCCCAAACGGCCCCCACCGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCtc                                      <  3:68524/136‑1 (MQ=255)                                                                                       ccGTCGTAGGCGGGGGTTCACCCGCTAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCtc                                      <  6:172052/135‑1 (MQ=255)                                                                                         gtcgtAGGAGCGGGTTAACCCGCGAAAGGGCCGCAACGCGCCCCCGGCGATCACAGGCCCGTTCCTACGCCCGATGCTCACCCACAAACCCTTTAACTTCAACCACCCGCCTGAAGCAATTCCAGACCGCCtcgt                                    >  2:313549/1‑135 (MQ=255)                                                                                                 cgggggTACCCCCGCTATCGGCCCGCACCGCGCCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAg                            <  5:290422/132‑1 (MQ=255)                                                                                                      gtccccccGCCAACGGCCCGCACCGCGCCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGcc                       <  1:273303/130‑1 (MQ=255)                                                                                                        tCACCCGCGAAAGGGCCGCCACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGt                     >  5:371973/1‑135 (MQ=255)                                                                                                        tCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGGCGATGTAGATGCCGtt                    >  3:244776/1‑136 (MQ=255)                                                                                                                  acgggCCCCACGGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCt           <  6:117904/133‑1 (MQ=255)                                                                                                                  acggcccGCACCGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCt           <  6:377206/130‑1 (MQ=255)                                                                                                                  acggcccGCACCGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCt           <  1:337487/130‑1 (MQ=255)                                                                                                                  aaGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCCTCGATGTAGATTCCGTTCGGCCCGCTg          >  8:93593/1‑136 (MQ=255)                                                                                                                           aaCGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTGCCACCTTc  <  1:313260/135‑1 (MQ=255)                                                                                                                            |                                                                                                                                      CGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTGCCACCTTC  >  ACB122/797408‑797664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑