Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	864,414	C→T	5.3%	D24N (GAT→AAT)	CJ019_00828 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	864,414	0	C	T	5.3%	242.6 / 2.9	75	D24N (GAT→AAT)	CJ019_00828	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (44/27);  new base T (2/2);  total (46/29)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

GCCGCTTCTATGTGGAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAGGGTCGCCTGTATGGATGACGAGCGGATGACA  >  ACB122/864281‑864546                                                                                                                                      |                                                                                                                                     gccgcTTCTATGTGGAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCg                                                                                                                                     <  5:317999/135‑1 (MQ=255)   cgcTTCTATGTGGAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCTGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGAt                                                                                                                                   >  7:115847/1‑135 (MQ=255)   cgcTTCTATGTGGAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGAt                                                                                                                                   >  4:225553/1‑135 (MQ=255)   cgcTTCTATGTGGAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGAt                                                                                                                                   >  8:323436/1‑135 (MQ=255)   cgcTTCTATGTGGAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGAt                                                                                                                                   >  3:124912/1‑135 (MQ=255)   cgcTTCTATGTGGAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGAt                                                                                                                                   >  7:203756/1‑135 (MQ=255)   cgcTTCTATCTGGAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGAt                                                                                                                                   >  7:362248/1‑135 (MQ=255)        cTATGTGGAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAgcgc                                                                                                                             >  8:352424/1‑136 (MQ=255)        cTATGTGGAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAgcg                                                                                                                              >  1:47889/1‑135 (MQ=255)        cTATGTGGAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTCGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAgcg                                                                                                                              >  5:344261/1‑135 (MQ=255)        cTATGTGGAAATCAAGCAAGCTGAACACAGTGCCGGCATCCACTTCGGGCAAGTACTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACTTTGATGGCAAAACCATCGATCAgcgc                                                                                                                             >  3:133810/1‑136 (MQ=255)              ggAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCtgttg                                                                                                                       >  4:308493/1‑136 (MQ=255)               gAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCtgttgt                                                                                                                      <  3:242343/136‑1 (MQ=255)                     cAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATc                                                                                                                 <  7:323436/135‑1 (MQ=255)                     cAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATc                                                                                                                 <  7:240607/135‑1 (MQ=255)                      aGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATc                                                                                                                 <  2:237755/134‑1 (MQ=255)                          aGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGctct                                                                                                            <  6:38155/135‑1 (MQ=255)                          aGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGctct                                                                                                            >  1:112923/1‑135 (MQ=255)                            cTGAACCCAGTGCCGGCATCCACTTCTGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTggg                                                                                                         <  8:347044/136‑1 (MQ=255)                            cTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTggg                                                                                                         <  8:362248/136‑1 (MQ=255)                             tGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTggg                                                                                                         <  6:189209/135‑1 (MQ=255)                               aaCCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGc                                                                                                        <  4:46906/134‑1 (MQ=255)                                aCCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCa                                                                                                       <  5:7304/134‑1 (MQ=255)                                       gCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCg                                                                                                                                     <  4:315421/97‑1 (MQ=255)                                       gCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCg                                                                                                                                     >  3:315421/1‑97 (MQ=255)                                        ccGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTag                                                                                              <  4:124912/135‑1 (MQ=255)                                         cGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAACCCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTagag                                                                                            >  8:328376/1‑136 (MQ=255)                                         cGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTagag                                                                                            >  3:159979/1‑136 (MQ=255)                                         cGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTaga                                                                                             >  5:396044/1‑135 (MQ=255)                                            cATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCg                                                                                         <  3:199314/136‑1 (MQ=255)                                             aTCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCg                                                                                         <  5:168626/135‑1 (MQ=255)                                              tCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCTTCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCgg                                                                                        >  1:378959/1‑135 (MQ=255)                                              tCCACTTCGGGCAAGTCCTCATGCACATGTCAGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCTGt                                                                                       >  3:91969/1‑136 (MQ=255)                                                   ttCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTgg                                                                                  >  3:91217/1‑136 (MQ=255)                                                   ttCGGGCAAGTCCTCATGCACATGTCAGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAACCCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTg                                                                                   >  6:191753/1‑135 (MQ=255)                                                     cGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTggg                                                                                                         <  1:150484/111‑1 (MQ=255)                                                     cGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTggg                                                                                                         >  2:150484/1‑111 (MQ=255)                                                          aaGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCg                                                                            >  6:340303/1‑135 (MQ=255)                                                            gTCCTCCTGCACACGCCGGCCACCCGCACAGCAGACCCGCCACTGGCGGTCCTGCACGTTGCTGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGc                                                                          <  1:177448/135‑1 (MQ=255)                                                               cTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTg                                                                       >  4:175329/1‑135 (MQ=255)                                                                     cacaTGTCCGCCAAGCGCACAGTAGCCCAGCCCCTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACagg                                                                >  7:324855/1‑134 (MQ=255)                                                                            ccGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCt                                                                                                                           >  2:155105/1‑70 (MQ=255)                                                                            ccGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCt                                                                                                                           <  1:155105/70‑1 (MQ=255)                                                                             cGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCt                                                        >  4:147394/1‑136 (MQ=255)                                                                             cGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGGTTTCGGCGTGCAACATGAAAAAGc                                                         >  2:99644/1‑135 (MQ=255)                                                                                  aGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCGGGTTTTCGGCGTGCTACATGAAAAAGCTCCgg                                                    >  1:270781/1‑135 (MQ=255)                                                                                       acaGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCg                                                                         <  2:18/109‑1 (MQ=255)                                                                                       acaGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCg                                                                         >  1:18/1‑109 (MQ=255)                                                                                       acaGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGtcatc                                              <  8:115847/136‑1 (MQ=255)                                                                                             gACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAgg                                        <  7:24203/136‑1 (MQ=255)                                                                                                cAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTc                                      <  5:340303/135‑1 (MQ=255)                                                                                                      cTGGCGGTCCTGCCCGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGGAGAGGCGGGCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAggg                               >  3:323134/1‑136 (MQ=255)                                                                                                      cTGGCGGTCCTGCACGTTGATGGCAAAACCATTGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAggg                               >  3:270025/1‑136 (MQ=255)                                                                                                      cTGGCGGTCCTGCACGTTGATGGCAAAACCATTGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAggg                               >  3:275059/1‑136 (MQ=255)                                                                                                      cTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCtgttgt                                                                                                                      <  8:208584/49‑1 (MQ=255)                                                                                                      cTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCtgttgt                                                                                                                      >  7:208584/1‑49 (MQ=255)                                                                                                      cTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAgg                                        >  8:130307/1‑127 (MQ=255)                                                                                                      cTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAgg                                        <  7:130307/127‑1 (MQ=255)                                                                                                      cTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAggg                               >  3:317490/1‑136 (MQ=255)                                                                                                      cTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAggg                               >  4:133300/1‑136 (MQ=255)                                                                                                      cTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAggg                               >  8:79599/1‑136 (MQ=255)                                                                                                      cTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAgg                                >  6:225087/1‑135 (MQ=255)                                                                                                      cTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAgg                                >  2:117097/1‑135 (MQ=255)                                                                                                        ggCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAGGGt                              >  5:208762/1‑135 (MQ=255)                                                                                                        ggCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGACATCAAAATGTCGACAGGGt                              >  2:25876/1‑135 (MQ=255)                                                                                                         gCGGTCCTGCACGTTGATGGCAAAACCATTGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAGGGt                              <  4:270025/134‑1 (MQ=255)                                                                                                         gCGGTCCTGCACGTTGATGGCAAAACCATTGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAGGGt                              <  4:275059/134‑1 (MQ=255)                                                                                                            gTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACACGGTAGcc                          >  1:179130/1‑135 (MQ=255)                                                                                                                tGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAGGGTCGCCTGTAt                     >  8:935/1‑136 (MQ=255)                                                                                                                    cGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAGGGTCGCCTGTATGGa                  >  1:288587/1‑135 (MQ=255)                                                                                                                    cGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAGGGTCGCCTGTATGGAt                 <  4:317490/136‑1 (MQ=255)                                                                                                                          tGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAGGGTCGCCTGTATGGATGACGa            >  2:304408/1‑135 (MQ=255)                                                                                                                          tGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCAGGTTTTCGGCCTGCAACAAGAAAACGCTCCGGGACATCAAAAGCTCGCCAGGGTCGCCTGTAGGGATGACGAg           >  8:192985/1‑136 (MQ=255)                                                                                                                                aaCCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAGGGTCGCCTGTATGGATGACGAGCGGAt      <  6:396044/135‑1 (MQ=255)                                                                                                                                   cATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAGGGTCGCCTGTATGGATGACGAGCGGATGACa  <  7:935/136‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     GCCGCTTCTATGTGGAAATCCAGCAAGCTGAACCCAGTGCCGGCATCCACTTCGGGCAAGTCCTCATGCACATGTCCGCCAAGCGCACAGTAGACCAGCCACTGGCGGTCCTGCACGTTGATGGCAAAACCATCGATCAGCGCCTGTTGTTCATCGCTCTGGGCAACGAGGTAGAGGCGGTCCTGGTTTTCGGCGTGCAACATGAAAAAGCTCCGGGTCATCAAAAGGTCGACAGGGTCGCCTGTATGGATGACGAGCGGATGACA  >  ACB122/864281‑864546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑