Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	930,475	T→G	21.9%	T2733P (ACC→CCC)	CJ019_00881 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	930,475	0	T	G	21.9%	92.2 / 4.3	65	T2733P (ACC→CCC)	CJ019_00881	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (15/34);  new base G (13/3);  total (28/37)
Fisher's exact test for biased strand distribution p-value = 9.17e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGACACTGCCCAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGCCCTCGGC  >  ACB122/930347‑930598                                                                                                                                 |                                                                                                                            cacGACACTGCCCAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCGCCGGGACCGGCGCCCCTGATTGGCAGATGGCTTGGCGGCCCCGGCGCGgtggtggc                                                                                                                       >  6:144330/1‑133 (MQ=21)    gACACTGCCCAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGGGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGt                                                                                                                   <  4:321449/136‑1 (MQ=255)     actcggCCCAGTCCTGTGGTGCCCGGGGGGGCGAGCACACGGAGGGTGCTGGCGGGTTCGGCGGGGCGGGGGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCAGAGGCGCGGTGATGTCTGGt                                                                                                                   <  2:161558/130‑1 (MQ=255)      cacTGCCCAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGCTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCCTCGCCCCTGCTTGGCAGCTCGCTTGGCGCCCCAGGCGCCGTGGTGTCTGGTgc                                                                                                                 >  4:74499/1‑136 (MQ=25)            ccAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCGACGGTACCGTCGCCACAGATTGCCAGATCCCTTGGCGCCACAGGCGCCGGGCTTGCTGGTgcggcgc                                                                                                            >  5:271527/1‑134 (MQ=37)            ccAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGCCGGTACCGGCGCCACAGATTGGCACCTCGCGTGGCGCCACCCGCGCGGTGTTGTGTGGTtcggcgc                                                                                                            >  1:36370/1‑134 (MQ=21)              aGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGCCGGTACCGTCGCCCCAGATTGGCAGATCGCGTGGCGCCACCGGCGCGGGGGTGTCTGGTGCGGCGGcc                                                                                                          >  5:163298/1‑135 (MQ=25)              aGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGCCCGTTCCGTCGCCCCTGATTTGCAGAACGCATGGGGCCCCAGGCGCGGTGATGTGTGGTTCGGCGccc                                                                                                          >  1:220617/1‑135 (MQ=21)              aGTACAGAGGTGCCATCGCCGGCCAGCACACGGACGGTGCTGCCTGCTTCGCCGCGGCCGCTGCCGACCGTACCCACCCCACTGAATGGCAGAACCCTTTGCGCCCCCGGCGCGGTGGATTCTGGTGCCGCCAc                                                                                                           >  3:283783/1‑134 (MQ=255)               gTACTGTGGTGCCTGCGGCGGCCTGCACAGGGACGGGGCTGCCGGCGGGGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCGTGGCGCCACAGGCGCGGTGATGTCTGGGGCGGCGACCTg                                                                                                        <  7:225701/136‑1 (MQ=255)                   aGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCGGCCGCCGGTACCGGCGCCCCGGATTGCCAGCTCGCTTGGCGCCCCCGGCGCGGGGGGGTCTGGTGCGGCGGCCAGccc                                                                                                     >  1:36943/1‑133 (MQ=21)                        tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCGCCGGTTCCGTCGCCACGGATTGACATCTCGCGTTGCGCCACCGGCGCGGTGCTGTATGGGGCCGGGACCTGCCCCGGcca                                                                                               >  4:19175/1‑134 (MQ=21)                        tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCGACGGTACCGTCGCCCCAGATTGCCAGATCGCTTGGCGCCCCAGGCGCGGGGATGTCTGGTGCGGCGcccggccccggccc                                                                                               >  7:251879/1‑134 (MQ=37)                        tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGCCGGTACCGTCGCCCCTGCTTGCCAGCTCGCTTTGCGCCACAGGCGCGGTGATGTCTGGTTCGGCGACCTGCACCCGcc                                                                                                >  5:193139/1‑134 (MQ=25)                        tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGCCGGGACCGGCGCCCCTGATTGGCAGATCGCTTGGCGCCCCCGCCGCGGGGGTGTCTTGTGCGGCGACCCGCCCCCGcc                                                                                                >  5:114132/1‑134 (MQ=21)                        tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACGGATTGCCAGATCGCTTGGCGCCCCAGGCGCGGGGATGTCTGGTGCGGCGCCCAGCCCCGGcca                                                                                               >  3:268720/1‑134 (MQ=37)                             tCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCCACGGTACCGTCGCCACAGATTGCCAGATCGCTTGGCGCCCCAGGCGCGGGGCTGTCTGGGGCGGCGCCCTGCACCGGCCacgac                                                                                           >  1:327198/1‑135 (MQ=37)                             tCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGCCGGGACCGTCGCCCCGGATTTCCAGCTCGCGTTGCGCCACCGGCGGCGGGGTGTGTGGTGCGGCGCCCAGCCCCGGCCacccc                                                                                           >  6:369263/1‑132 (MQ=16)                             tCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCCCTGATTGCCAGATCGCTTGGCGCCCCCGGCGCGGGGATGTCAGGTGCGGGGGCCGGCACCCGCAacgcc                                                                                           >  1:313092/1‑133 (MQ=37)                             tCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAacgacg                                                                                          >  8:380384/1‑136 (MQ=255)                                gcggcCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGCCGGTTCCGTCGCCCCGGCTTGGCCGCTCGCGTGGCGCCCCCGGCGCGGGGGTGTCTGGTGCGGCGGCCGGCACCGGCCacgccgcg                                                                                        >  1:300877/1‑133 (MQ=255)                                  ggcCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTGTGGTGCGGCGACCGGCCCCGGCCACGACGAGGt                                                                                      >  2:243460/1‑135 (MQ=255)                                               ccggGGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGa                                                                        <  3:88399/135‑1 (MQ=255)                                                    gcggcctgcTTGGGGGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGcc                                                                   <  7:170381/133‑1 (MQ=255)                                                     ctgcctgcTTCGGCGCGGCCGCTGCCGACGGTACCGTCGCCCCGGCTTGCCAGATCGCTTGGCGCCACCGGCGCGGTGCTGTCTGGGGCGGCGACCTGCACCGGCCACGACGCGGTGCCGTTGTTGTCGATCGcc                                                                   >  1:39706/1‑135 (MQ=255)                                                     ctgcctgcTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCCCGGAGTGGCAGATCGCTTGGCGCCACACGCGCGGGGGTGTCTGGGGCGGCGCCCTGCACCGGCCACCACCAGGTGGCGTTGTTGTCGATCGcc                                                                   >  1:256011/1‑135 (MQ=255)                                                     cggccggcGTCGGCGCGGCCGCTGACGACGGTACCGGCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGcc                                                                   <  6:242949/133‑1 (MQ=255)                                                                ggCGCGGCCGCTGCCGCCGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCCCAGGCGCGGTGATGTCTGGTGCGGCGGCCTGCACCGGCAACGACGAGGTGCCGTTGGTGTCGATCGCCCCCACGTcccg                                                        >  6:114364/1‑133 (MQ=255)                                                                ggCGCGGCCGCTGCCGACGGTTCCGGCGCCCCTGATTGGCAGATCGCTTGGCGCCACCGGCGCGGGGGTGTGTGGTGCGGCGCCCTGCCCCGGCCACCACGCGGTGCCGGTGTTGTCGCTCGCCCCCCCGTCCCGc                                                       >  4:141082/1‑136 (MQ=255)                                                                ggCGCGGCCGCTGACGCCGGTACCGTCGCCACGGATTGGCAGGTCGCTTGGCGCCCCAGGCGCGGGGATGTCTGGTGCGGCGGCCTGCACCGGCCACGCCGAGGTGCCGTTGTTGTCGCTCGCCCCCCCGTCCAGc                                                       >  3:159633/1‑136 (MQ=255)                                                                ggCGCGGCCGCTGACGCCGGTACCGGCGCCCCGGATTGCCAGCTCGCTTGGCGCCCCAGGCGCGGTGCTGTCTGGTGCGGCGCCCTGCACCGGCAACGACGCGGTGCCGTTGTTGTCGCTCGCCACCACGTCCCGc                                                       >  8:222209/1‑136 (MQ=255)                                                                 gcgcGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCa                                                      <  7:63449/136‑1 (MQ=255)                                                                  cgcgGCCGCTGACGACGGTACCTTCGCCACTGATTGACAGATCGCTTGGCGCCACTGGGGCTGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCTCGTCCAGCa                                                      <  2:121903/135‑1 (MQ=255)                                                                       gcGCTGAGGACGGTACGGTCGCCTCTGATTGCCAGATCGCTTGGCGCTACAGGCGCGGTGATGTCTGGGGGGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCg                                                 <  2:94214/134‑1 (MQ=255)                                                                       cggCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCg                                                 <  6:382142/133‑1 (MQ=255)                                                                       ccGCTGACGAGGGTACCGTCGCAACTGATTGCCAGAGCGCTGTGCGCCTCAGGGGCGGTGATGTCTGGTGCGTCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCg                                                 <  6:179457/135‑1 (MQ=255)                                                                                acgGTACCGTCGGCTCTTATTGGCAGTTCGCTTGGCGCCAGAGGCGAGGTGATGTGTGGTGCGGCGAGCTGCACCGGCTACGACGTGGTGCCGTTGTTGTCGATCGCCACCACGTGCAGCAACTCGCCATTGGCCt                                       <  4:283783/136‑1 (MQ=255)                                                                                acgGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                       <  3:385861/136‑1 (MQ=255)                                                                                acgGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGATGTGATTTATGGTGCGTCTACCTGCACCGGCAACGACGAGTTGACGTTGTTGTCGATCGCCACCACGTCCAGCAAATCTCCATTGGCCt                                       <  4:278681/136‑1 (MQ=255)                                                                                acgGTACCGTCGCCACTGATTGCCAGAGCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGGTGTTGTCGATCTCCACCACGTCCAGCAACTCGCCATTGGCCt                                       <  8:258038/136‑1 (MQ=255)                                                                                 cgggcccGGCGCCAGTGATGGCCAGAGCGCTGGGCGCCCCCGGCGCGGGGCTGGCTGGGGCGGCGCCCTGCCCCGGCACCGACGAGGTGCCGGTGGTGTCGATCGCCACCACGTCCAGCAACTCGCCCTTGGCCt                                       <  5:282241/130‑1 (MQ=255)                                                                                 cgGTACCGTCGCCACTGCTTGCCAGATCGCTTGGCGCCACAGGGGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTg                                      <  4:182186/136‑1 (MQ=255)                                                                                 cgGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                       <  1:215684/135‑1 (MQ=255)                                                                                 cgGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTg                                      <  4:268720/136‑1 (MQ=255)                                                                                 cgGTACCGGCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                       <  6:114132/135‑1 (MQ=255)                                                                                 cgGTACCGGCGCCACTGATTGCCAGAGCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                       <  2:300877/135‑1 (MQ=255)                                                                                 cgGTACCGGCGCCACTGAGTGCCAGAGCGCTGGGCGCCCCAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                       <  5:282240/135‑1 (MQ=255)                                                                                  ggTACCGTCGCGAGTGATTGCCAGATCGATTGGCGCCAGAGGCGGGGTGATGTCTGGTGCGGCGACCTGCACGGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTgg                                     <  3:74499/136‑1 (MQ=255)                                                                                  ggTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                       <  2:36370/134‑1 (MQ=255)                                                                                   gTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGGGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTgg                                     <  6:311770/135‑1 (MQ=255)                                                                                            ccACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                       <  1:341741/124‑1 (MQ=255)                                                                                            ccACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                       >  2:341741/1‑124 (MQ=255)                                                                                            ccACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGta                              >  5:46997/1‑133 (MQ=255)                                                                                             cACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAg                          <  7:292309/136‑1 (MQ=255)                                                                                             cACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAg                          <  7:380384/136‑1 (MQ=255)                                                                                              aCTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAg                          <  2:327198/135‑1 (MQ=255)                                                                                               cTGATTGCCAGAGCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGca                        <  4:346634/136‑1 (MQ=255)                                                                                                 gATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGcacc                      >  7:250942/1‑136 (MQ=255)                                                                                                  aTTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGcacc                      <  8:251879/135‑1 (MQ=255)                                                                                                   ttGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGcacc                      <  5:165002/134‑1 (MQ=255)                                                                                                     gCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACg                   <  8:250942/135‑1 (MQ=255)                                                                                                               ttGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGc         <  2:36943/135‑1 (MQ=255)                                                                                                                 ggCGCCACAGGCGCGCTGATGTCTGGTGCGGCGACCTGCACCGGCACCGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCCGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGccc       >  1:265911/1‑135 (MQ=255)                                                                                                                  gcgcCACAGGCGCGGGGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGccc       <  6:271527/134‑1 (MQ=255)                                                                                                                     cccccggCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGAGCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGGGAAGGTGCCCTCGGc  <  7:125496/131‑1 (MQ=255)                                                                                                                     ccACCGGCGCGGTGCTGTCGGGTGCGGCGACCTGCACCGGCAACGACGAGGGGCCGTTGTTGTCGAGCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGCCCTCGGc  <  4:279180/136‑1 (MQ=255)                                                                                                                                 |                                                                                                                            CACGACACTGCCCAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGCCCTCGGC  >  ACB122/930347‑930598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑