| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 955,841 | T→G | 26.6% | L84R (CTG→CGG) | phnD1 → | putative ABC transporter phosphite bindingprotein PhnD1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 955,841 | 0 | T | G | 26.6% | 41.5 / 2.6 | 37 | L84R (CTG→CGG) | phnD1 | putative ABC transporter phosphite bindingprotein PhnD1 |
| Reads supporting (aligned to +/- strand): ref base T (10/16); new base G (3/8); total (13/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGCAAGTTCAAGCCTTTGGGTGAGTACCTGGCCAAGCAACTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTTCACGTTCGTTCAGGTGCACCTGAAGGACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGACGCACAGTTCACCTCCAAGTTCATCACCGCCAACCCCGACGTGAAGAGCCTGAGCGATC > ACB122/955712‑955972 | aGCGCAAGTTCAAGCCTTTGGGTGAGTACCTGGCCAAGCAACTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTggggg < 6:143645/135‑1 (MQ=255) gTTCAAGCCTTTGGGTGAGTACATGGCCAAGCAACTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGCCCCCCACCGACAGGCCGGGCGGGGGGGCGTCCcg > 8:140841/1‑136 (MQ=255) ccgttGGGTGAGTACCTGGCCAAGCAACTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTTCACgttcgttc < 4:292749/133‑1 (MQ=255) cTTTGGGTGAGTACCTGGCCAAGCAACTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGAACATGCCTGGCGGGGGGGCGTGAcgttcgtt > 3:208545/1‑134 (MQ=255) gggTGAGTACCTGGCCAAGCAACTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTTCACGTTCGTTCAGGt < 3:223044/135‑1 (MQ=255) ggTGAGTACCTGGCCAAGCAACTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTTCACGTTCGTTCAGGt < 2:76414/134‑1 (MQ=255) ttGAGTACCTGGCCAAGCAACTGGGCATGGAGGTGAATTTCGTACCCGTGGAGGACTACCCGGCGGTGGTCGAGTAGCTGGCCGCAGACCGCCTAGCTCTGGCCTGTCTGGGGGGGGTCACATTCCTTATGGTg > 5:21223/2‑134 (MQ=255) gTGAGTACCTGGCCAAGCAACTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGCCCGCCTCGCTCTGGCCTGGCTGGGGGGGTTCACCTTCGTTCCGGGGc > 1:221485/1‑135 (MQ=255) aCCTGGCCAAGCAACTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCCGCCCGCCGCGCTCTGGGCTGGCGGGGGGGCGTGACGGTCGTTAAGGTGCAccg > 1:190576/1‑132 (MQ=255) aCCTGGCCAAGCAACTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCGTCACGTTCGTTCAGGTGCACCt > 5:89172/1‑133 (MQ=255) aCCTGGCCAAGCAACTGGGAATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCCCTGGCCCCAGCCCCCCGCGCTCTGGCCTGGCTCGGGGGCGTCCAGGTCGTTAACGTggcactg > 3:69023/1‑128 (MQ=255) ccTGGCCAAGCAACTGGGCATGGCGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTTCACGGTCGTTCAGGTGCACCTGAAg > 8:77094/1‑136 (MQ=255) ccACGCAACTGGGCAGGGAGGTGAAGGTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTTCACGTTCGTTCAGGTGCACCTGAAGGa < 5:69218/133‑1 (MQ=255) aaCTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTTCCCGTTCGTTCAGGTGCACCTGAAGGACCCGACcgc > 7:258347/1‑136 (MQ=255) aaCTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTTCACGTTCGTTCAGGTGCACCTGAAGGACCCGACcg > 5:154904/1‑135 (MQ=255) aaCTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGGCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTTCACGTTCGTTCAGGTGCACCTGAAGGACCCGACcgc < 8:50566/136‑1 (MQ=255) ccTGGGCAGGGAGGTGAAGTCCGACCCCGTGGCTGACTACCCGGCGGTGGTCGAGGCGCGGGCGGCAGACCGCGTCGATCTGCCCTGGCGGGGGGGCTCCAGGTTCGTTCAGGTGCACGTGAAGGACCCGACcgc < 6:154071/134‑1 (MQ=255) ggggCATGGCGGTGACGGTCGTCCCCGGGGCGGACTCCCCGGCGGGGGGCGAGTCGCGGGCCGCAGACCGCCTCGAGCGGGCCTGGCGGGGGGGCTGCACGTTCGTTCAGGTGCACCTGAAGGACCCGACcgcca < 1:335228/134‑1 (MQ=255) ggactGGAGGTGAAGTTCGAACCCGGGGCGGACTACCCGGCGGTGGTCGAGGCGCTGGCCGCAGACCGCCTCGATCGGGCCTGGCGGGGGGGCTTCACGTTCGTTCCGGTGCCCCTGAAGGACCCGACcgccacg < 2:333437/131‑1 (MQ=255) ggCATGGAGGGGAAGTTCGTACCGGGGGCGGACTACCGGGCGGTGGGCGCGGCGCTGGCCGCAGACCGCCTCGATCTGGCCGGGGTGGGGGGGTTCACGTTCGTTCAGGTGCACCTGAAGGACCCGACcgccacg < 5:105804/135‑1 (MQ=255) gTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCGGGGGGGCTTCACGTTCGTTCCGGTCCACCTGAAGGACCCGACCGCCACGCCACTGGTAc < 7:34743/136‑1 (MQ=255) aGTGCGTACCCGGGGCGGACTACCCGGCGGTGGGCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTTCACGTTCGTTCAGGGGCACCTGAAGGACCCGACCGCCACGCCACTGGTACAGCg < 8:393949/136‑1 (MQ=255) ccgcccccGGGGCGCACTACCCGGCGGGGGCCGAGGCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCGGGGGGGCTTCACGTGCGTTCAGGTCCACCTCAAGGCCCCGACCGCCACGCCACTGGTACAGCGTg < 1:365352/130‑1 (MQ=255) aCCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCtt > 5:153092/1‑75 (MQ=255) aCCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCtt < 6:153092/75‑1 (MQ=255) ggTGGCGGACTACCCGGGGGTGGGCGAGGCGCTGGCGGCAGACCGCGGGGAGCTGGCCTGGCGGGGGGGCTTCACGTTGGTGCCGGTGCACCGGAAGGACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGAc < 3:346037/135‑1 (MQ=255) cggggcggcCTACCCGGCGGTGGTCGAGGCGCGGGCCGCAGACCGCCTCGATCGGGCCTGGCGGGGGGGCTTCACGTGCGTTCAGGGGCACCTGAAGGACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGAc < 7:186347/133‑1 (MQ=255) ggggCGGACTACCCGGCGGTGGGCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTGCACGTTCGTTCAGGTGCACCTGAAGGACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGAc < 5:332804/133‑1 (MQ=255) cccGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTTCACGTTCGTTCAGGTGCACCTGAAGGACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGACGCACAGTTCAcc < 4:208545/136‑1 (MQ=255) gAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCGTCACGTTCGTTCAGGTGCACCTGAAGGACCCGACCGCCACGCCACTGGTTCCGCGTGAGCAGGCCGCCCAGTTGACCTCCAAGTtcatc > 2:14425/1‑135 (MQ=255) aggcGCTGGCGGCAGACCGCCTCGTTCTGGCGTGGCTGGGGGGCTTCACGTTCGTTCAGTTGCACCTGAAGGACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGACGCACAGTTCACCTCCAAGTTCATCAc < 3:44974/133‑1 (MQ=255) aGACCGCCTCGATCGGGCCTGGCGGGGGGGCTTCACGTGCGTTCAGGGGCACCTGAAGGACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGACGCACAGTTCACCTCCAAGTTCATCACCGCCAACCCCGAc < 3:3243/136‑1 (MQ=255) ccGCCTGGATCTGGCCTGGTTGGGGGGCTTCACGTTGGTGCCGGTGCACCTGATGCACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGACGCACAGTTCACCTCCAAGTTCATCACCGCCAACCCCGACGt < 2:190576/135‑1 (MQ=255) ccGCCTCGAGCGGGCCGGGCTGGGGGGCTTCACGTTCGTTCAGGTGCCCCTGAAGGACCCGACGGCCACGCCACTGGTAGAGCGTGAGCAGGACGCACAGTTCACCTCCAAGTTCATCACCGCCAACCCCGACGt < 6:324346/135‑1 (MQ=255) tCGATCTGGCCTGGCTGGGGGGCTTCACGTTCGTTCAGGTGCACCTGAAGGACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGACGCACAGTTCACCTCCAAGTTCATCACCGCCAACCCCGACGTGAaga > 5:136529/1‑135 (MQ=255) cctggcTGGGGGGCTTCACGTTCGTTCAGGTGCACCTGAAGGACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGACGCACAGTTCACCTCCAAGTTCATCACCGCCAACCCCGACGTGAAGAGCCt < 2:11075/130‑1 (MQ=255) cctggcTGGGGGGCTTCACGTTCGTTCAGGTGCACCTGAAGGACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGACGCACAGTTCACCTCCAAGTTCATCACCGCCAACCCCGACGTGAAGAGCCt < 5:330448/130‑1 (MQ=255) tggctggGGGGCTTCACGTTCGTTCAGGTGCACCTGAAGGACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGACGCACAGTTCACCTCCAAGTTCATCACCGCCAACCCCGACGTGAAGAGCCTGAGCGATc > 4:209688/1‑136 (MQ=255) | AGCGCAAGTTCAAGCCTTTGGGTGAGTACCTGGCCAAGCAACTGGGCATGGAGGTGAAGTTCGTACCCGTGGCGGACTACCCGGCGGTGGTCGAGTCGCTGGCCGCAGACCGCCTCGATCTGGCCTGGCTGGGGGGCTTCACGTTCGTTCAGGTGCACCTGAAGGACCCGACCGCCACGCCACTGGTACAGCGTGAGCAGGACGCACAGTTCACCTCCAAGTTCATCACCGCCAACCCCGACGTGAAGAGCCTGAGCGATC > ACB122/955712‑955972 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |