Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,021,440 A→T 5.5% F117Y (TTC→TAC)  dppA_3 ← Periplasmic dipeptide transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,021,4400AT 5.5% 258.4 / 4.0 83F117Y (TTC→TAC) dppA_3Periplasmic dipeptide transport protein
Reads supporting (aligned to +/- strand):  ref base A (41/36);  new base T (3/2);  total (45/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

TTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCGTCGTCGGACACTTCCCATTTGGTC  >  ACB122/1021308‑1021562
                                                                                                                                    |                                                                                                                          
ttCACCGTGTGCTCGTCAAGCTTCTCCACCTTCGCAATGTTCTTGTCCATGCCCATGTAGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACa                                                                                                                         >  4:30298/1‑136 (MQ=255)
       tgtgCTCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCAcgt                                                                                                                   <  1:371979/135‑1 (MQ=255)
          gCTCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACTCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCAcgtcgt                                                                                                                >  5:51675/1‑135 (MQ=255)
          gCTCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCAcgtcgt                                                                                                                >  1:213758/1‑135 (MQ=255)
          gCTCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCAcgtcgt                                                                                                                >  1:366294/1‑135 (MQ=255)
          gCTCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACgtcgtc                                                                                                               >  7:37488/1‑136 (MQ=255)
          gCTCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACgtcgtc                                                                                                               >  7:196402/1‑136 (MQ=255)
            tCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGATGTATGGAAAGTCGGTGGGGTACGCGTTGCGGCAGGGGTTGTCGTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGtcgtcg                                                                                                              >  5:3604/1‑135 (MQ=37)
            tCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAATGTGAACAGCCCGTCGTCgg                                                                                                             >  8:234739/1‑136 (MQ=255)
            tCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCgg                                                                                                             >  4:153088/1‑136 (MQ=255)
            tCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCgg                                                                                                             >  7:240616/1‑136 (MQ=255)
            tCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGAGGAACGGGTTGTCCTTTTCGAGCATGCGGTTGAAGGTGATCAGCACGtcgtcg                                                                                                              >  2:317778/1‑135 (MQ=255)
            tCGTCAAGCTTCTCCACCTTGGCAATGTTATTGTCAATGAACATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGACTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGCACAGCACGtcgtcg                                                                                                              >  2:321627/1‑135 (MQ=37)
                  aGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTg                                                                                                        >  3:204323/1‑135 (MQ=255)
                          cACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGTGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCTTGGGGTTGATGGTGAACAGCACGTCGTCTGCGTTGAACGGGc                                                                                                 <  3:50584/134‑1 (MQ=255)
                          cACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGc                                                                                                 <  4:1268/134‑1 (MQ=255)
                           aCCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGa                                                                                               >  7:73720/1‑135 (MQ=255)
                           aCCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACATCGTCGGCGTTGAACGGGCGa                                                                                               >  2:94745/1‑135 (MQ=255)
                              ttGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACCTCGTCGGCGTTGAACGGGCGACTg                                                                                            <  1:94745/135‑1 (MQ=255)
                                ggCAATGTTCTTGTCCTTGCCCAAGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTcgg                                                                                          >  5:99838/1‑135 (MQ=255)
                                  cAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCtt                                                                                       <  8:196402/136‑1 (MQ=255)
                                  cAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTAGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCt                                                                                        >  6:48807/1‑135 (MQ=255)
                                    aTGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCtt                                                                                       >  2:372835/1‑134 (MQ=255)
                                        tCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGaa                                                                                    >  6:178735/1‑133 (MQ=255)
                                          ttGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAagtagt                                                                               <  8:73720/136‑1 (MQ=255)
                                            gTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAagtagt                                                                               <  7:4477/134‑1 (MQ=255)
                                             tCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAagtagt                                                                               <  5:357358/133‑1 (MQ=255)
                                              ccATGCCCATGTCGGTGAACTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACCGGCGACTGGGCTTGAAGTAGTCggt                                                                           >  7:46187/1‑136 (MQ=255)
                                                 tGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCggtggt                                                                        <  7:238938/136‑1 (MQ=255)
                                                    ccATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGCACGGGCGACTGGGCTTGAAGTAGTCGGTGgtgt                                                                      >  2:85557/1‑135 (MQ=255)
                                                    ccATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGtgtg                                                                     >  3:370254/1‑136 (MQ=255)
                                                    ccATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGtgtg                                                                     >  7:305085/1‑136 (MQ=255)
                                                    ccATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGACTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGgtgt                                                                      >  2:333662/1‑135 (MQ=255)
                                                          cGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCggtg                                                                          <  3:220330/125‑1 (MQ=255)
                                                          cGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCggtg                                                                          >  4:220330/1‑125 (MQ=255)
                                                               aaGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGAccc                                                          >  4:112105/1‑136 (MQ=255)
                                                                aGTATGGTAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCGTTGTCGAGCATGCGGTTGAAGGTGATGAGCACGTCGTGGGCGTTGAACGGTCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGAcc                                                           <  2:261193/134‑1 (MQ=37)
                                                                aGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCggtg                                                                          <  3:271374/119‑1 (MQ=255)
                                                                aGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCggtg                                                                          >  4:271374/1‑119 (MQ=255)
                                                                    tGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTc                                                      <  3:153088/135‑1 (MQ=255)
                                                                     ggAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTcgc                                                    >  4:131044/1‑136 (MQ=255)
                                                                       aaaCTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGTACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGCACGTGACCCCTTcgc                                                    >  5:24987/1‑134 (MQ=255)
                                                                       aaaCTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTcgc                                                    >  2:212247/1‑134 (MQ=255)
                                                                       aaaCTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGAATGCGGTTGAAGGTGTACAGCACGTCGTCGGCGTTGAACCGGCGACTGGGCTTGAAGTAGTAGGTGGTGTGGAACGTGCCCCCTTagcg                                                   >  5:24984/1‑135 (MQ=255)
                                                                          cTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAgg                                               <  3:131044/136‑1 (MQ=255)
                                                                           tCGGTGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGt                                              <  1:151124/135‑1 (MQ=255)
                                                                             ggTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCTCAGGt                                              <  8:240616/134‑1 (MQ=255)
                                                                             ggTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGt                                              <  4:204323/134‑1 (MQ=255)
                                                                             ggTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGt                                              <  8:37488/134‑1 (MQ=255)
                                                                             ggTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGt                                              <  7:20198/134‑1 (MQ=255)
                                                                              gTGGGGTACGCCTTGCGGAACGGGTTTTCCTTGTCGAGCATGCGGTTGTAGGTGAACAGCACGTGGTGGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGGGTGGAACTTGACCCCTTCGCGCAGGt                                              <  5:185877/133‑1 (MQ=37)
                                                                              gTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGt                                              <  5:178735/133‑1 (MQ=255)
                                                                              gTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTg                                             >  2:183588/1‑134 (MQ=255)
                                                                              gTGGGGTACGCCTTGCGGAACGGGTTGACCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGt                                              <  1:132943/133‑1 (MQ=255)
                                                                               tGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTg                                             >  1:169028/1‑133 (MQ=255)
                                                                                 gggTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAgg                                        <  3:200074/136‑1 (MQ=255)
                                                                                              ggAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGtt                                                                                                         >  5:351324/1‑58 (MQ=255)
                                                                                              ggAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGtt                                                                                                         <  6:351324/58‑1 (MQ=255)
                                                                                              ggAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAgg                                               <  2:392437/116‑1 (MQ=255)
                                                                                              ggAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAgg                                               >  1:392437/1‑116 (MQ=255)
                                                                                               gAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGATCGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCcg                          <  3:30298/136‑1 (MQ=255)
                                                                                               gAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGcc                           >  5:225873/1‑135 (MQ=255)
                                                                                               gAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCcg                          >  8:192392/1‑136 (MQ=255)
                                                                                                    ggtggtCCTTGTCGAGCATGCGGTTGAAGGTTAACAGCACGTCGTCGGCGGTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCgtcgtc                     <  8:153732/136‑1 (MQ=255)
                                                                                                    ggTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGGCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCgtcgtc                     <  3:3490/136‑1 (MQ=255)
                                                                                                     gTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCgtcgtc                     <  1:14546/135‑1 (MQ=255)
                                                                                                     gTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCgtcgtc                     >  6:62658/1‑135 (MQ=255)
                                                                                                     gTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGGTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCgtcgtc                     <  2:260491/135‑1 (MQ=255)
                                                                                                       tGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCGTCGTCgg                   <  2:165295/135‑1 (MQ=255)
                                                                                                          ccTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGTAGGTGTAGGTCTTGCCGTCGGCGGgca                >  5:266318/1‑135 (MQ=255)
                                                                                                                 gAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTg                                                                                                        <  4:27166/40‑1 (MQ=40)
                                                                                                                 gAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTg                                                                                                        >  3:27166/1‑40 (MQ=40)
                                                                                                                 gAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAATTAGTCGGTGGTGTGGAACTTTACCCCTTCGCGCAGGTGGCAGGTGTATGTGTTGCCGTGGTCGGGCCCTTGCCa         >  2:199361/1‑135 (MQ=25)
                                                                                                                 gAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCGTCGTCGGACACTTCCCAt        >  7:43116/1‑136 (MQ=255)
                                                                                                                      tGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGGGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCGTCGTCGGACACTTCCCATTTGGt   <  3:361841/136‑1 (MQ=255)
                                                                                                                       gCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCGTCGTCGGACACTTCCCATTTgtt   >  2:129740/1‑133 (MQ=255)
                                                                                                                       gCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCGTCGTCGGACACTTCCCATTTGGt   <  1:372835/135‑1 (MQ=255)
                                                                                                                       gCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCGTCGTCGGACACTTCCCATTTGGt   <  2:213758/135‑1 (MQ=255)
                                                                                                                       gCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCGTCGTCGGACACTTCCCATTTGGt   <  6:204494/135‑1 (MQ=255)
                                                                                                                       gCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACCGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCGTCGTCGGACACTTCCCATTTGGt   >  5:191323/1‑135 (MQ=255)
                                                                                                                         ggctGAAGGTGTACAGCACGTCGGCGGCGTTGATCGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCGTCGTCGGACACTTCCCATTTGGTc  <  6:24987/131‑1 (MQ=255)
                                                                                                                           ttGAAGGTGTACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAagtagt                                                                               <  3:32830/55‑1 (MQ=255)
                                                                                                                           ttGAAGGTGTACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAagtagt                                                                               >  4:32830/1‑55 (MQ=255)
                                                                                                                                    |                                                                                                                          
TTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGTGAAGTATGGAAACTCGGTGGGGTACGCCTTGCGGAACGGGTTGTCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACGTCGTCGGCGTTGAACGGGCGACTGGGCTTGAAGTAGTCGGTGGTGTGGAACTTGACCCCTTCGCGCAGGTGGAAGGTGTAGGTCTTGCCGTCGTCGGACACTTCCCATTTGGTC  >  ACB122/1021308‑1021562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: