Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,102,967 T→G 6.7% I194S (ATC→AGC)  lutR_2 → HTH‑type transcriptional regulator LutR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,102,9670TG 6.7% 175.6 / 4.7 60I194S (ATC→AGC) lutR_2HTH‑type transcriptional regulator LutR
Reads supporting (aligned to +/- strand):  ref base T (22/33);  new base G (2/2);  total (25/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

GCTGGTCGATGCCGCCCACAACCCGGCATTAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATCCATCACCCGTAATTCCCCGTCGAACGAGCGGGATCTCGA  >  ACB122/1102833‑1103100
                                                                                                                                      |                                                                                                                                     
gCTGGTCGCTGCCGCCCACACCCCGGCAGTAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCCACACAATATGGCCCCCGGTACGCGCTGCCAGGCCACGTTCGTCTTGCGTGGGCGGATc                                                                                                                                      >  7:189019/1‑136 (MQ=255)
    gTCGATGCCGCCCACAACCCGGCATTAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCg                                                                                                                                  <  3:205574/136‑1 (MQ=255)
        aTGCCGCCCACAACCCGGCATTAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGc                                                                                                                              <  3:36928/136‑1 (MQ=255)
          gccgccCACAACCCGGCATTAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGc                                                                                                                              >  7:314211/1‑134 (MQ=255)
           ccgccCACAACCCGGCATTAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGTCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCa                                                                                                                            <  7:150910/135‑1 (MQ=255)
            cgccCACAACCCGGCATTAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATc                                                                                                                          <  7:121856/136‑1 (MQ=255)
                   aaCCCGGCATTAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCaa                                                                                                                    <  2:103329/135‑1 (MQ=255)
                           aTTAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATTGCCCCCGTTCCGCGCTGCCAGGCCACGGTCGACATGCATGGGCAGATCCTCGCCGCCATCGAGCCACGTGACGc                                                                                                            >  2:243105/1‑135 (MQ=255)
                           aTTAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATTGCCACCGTTCCGCGCTGCCACGCCCCGTTCGCCTTGCATGGGCAGATCCTCGACGCCATCGCGCAACGTGACGc                                                                                                            >  5:89495/1‑135 (MQ=255)
                           aTTAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCt                                                                                                           <  8:314211/136‑1 (MQ=255)
                             tAAGCGATCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCTACACAATATGGCCACCGTTCCGCGATGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCt                                                                                                           <  4:255931/134‑1 (MQ=255)
                             tAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTg                                                                                                          <  5:361490/135‑1 (MQ=255)
                                  gAGCTGTACCGTTACTTTTCCGGGGTCGGGGCTGCCGCTCTGCAACACAATATGGCCACCGATCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACggg                                                                                                    <  8:75667/136‑1 (MQ=255)
                                   tgCTGTACCGGTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGGTCCGCGCTGCCAGGACACGTTCGACTTGGATGGGCAGATCCTCGACGCCTTCGAGCAACGTGACGCTGAACggg                                                                                                    <  6:330104/134‑1 (MQ=255)
                                   aGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGGTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACggg                                                                                                    <  5:398685/135‑1 (MQ=255)
                                     cTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGcc                                                                                                  <  5:182209/135‑1 (MQ=255)
                                         aCCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAg                                                                                               <  1:212775/134‑1 (MQ=255)
                                          ccGGTACTATTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCAGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAgcg                                                                                             <  2:394427/135‑1 (MQ=255)
                                                 ttttCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGAc                                                                                       <  6:125929/134‑1 (MQ=255)
                                                   ttCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCg                                                                                    <  3:25231/135‑1 (MQ=255)
                                                     ccGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGACCCCTTCGACTTGCATGGTCAGAACCTCGACCCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCgga                                                                                  >  3:197960/1‑133 (MQ=255)
                                                      cGGGGTCGTGGCTGCCGCTCTGCAACAAAATATGGCCACCGTGCCGCGATGCCAGGCCACGTGCGACTTGCAGGGGCAGTTCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTa                                                                                  <  2:121343/134‑1 (MQ=255)
                                                           tCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCa                                                                            <  5:362162/135‑1 (MQ=255)
                                                                     ggcgctGCAACACAATATGGCCACGGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCTGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTg                                                                  <  6:20507/131‑1 (MQ=255)
                                                                        tctGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGCCGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTgagc                                                               >  5:83651/1‑134 (MQ=255)
                                                                        tctGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTgaga                                                               >  5:141664/1‑135 (MQ=255)
                                                                          tGCAACACATTATGGCCACCGTGCCGCGCTGCAGGGCCACGTCCGAGTGGCATGGGCAGATCCTCGACGCCTTCGAGCAACGTGACGGTGAACGGGCCAAGCGCCTTACGCGTATCGTCATTGAATCCTGAGAcc                                                             <  6:44487/135‑1 (MQ=255)
                                                                            cAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGAcc                                                             <  4:246385/133‑1 (MQ=255)
                                                                             aaCACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGAcc                                                             <  5:271212/132‑1 (MQ=255)
                                                                             aaCACAATATGGCCACCGTTCCGCGCGGCCAGGCCAGGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGAcc                                                             <  1:199453/132‑1 (MQ=255)
                                                                              acacAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGaa                                                        <  8:289324/136‑1 (MQ=255)
                                                                               cacaATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGAGCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGAc                                                                                       <  6:361407/104‑1 (MQ=255)
                                                                               cacaATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGAGCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGAc                                                                                       <  6:355561/104‑1 (MQ=255)
                                                                               cacaATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGAGCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGAc                                                                                       >  5:355561/1‑104 (MQ=255)
                                                                               cacaATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGAGCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGAc                                                                                       >  5:361407/1‑104 (MQ=255)
                                                                                 caATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAgg                                                      >  5:258171/1‑135 (MQ=255)
                                                                                    tatGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCa                                                   <  5:342978/135‑1 (MQ=255)
                                                                                    tatGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCa                                                   >  6:346361/1‑135 (MQ=255)
                                                                                     atGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCAt                                                  >  6:99557/1‑135 (MQ=255)
                                                                                      tGGCCACCGTTCCGCGCGGCGAGGCCAGGTGCGACTTGCATGGGGAGATCCTCGACGCCATGGAGCAACGTGACGCTGAAGGGGCCAAGCGCCTGACGCGTATCCTCATTGAATCCTGAGACCGAGAAGGCAATgg                                                <  4:90460/136‑1 (MQ=255)
                                                                                       ggCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATc                                                                                                                          >  5:289101/1‑61 (MQ=255)
                                                                                       ggCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATc                                                                                                                          <  6:289101/61‑1 (MQ=255)
                                                                                       ggCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATgg                                                >  6:139055/1‑135 (MQ=255)
                                                                                       ggCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGc                                               >  3:67693/1‑136 (MQ=255)
                                                                                              gTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCTAGAAGGCCATGGCTGAATcc                                        <  4:98403/136‑1 (MQ=255)
                                                                                               ttCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATcc                                        >  1:171005/1‑135 (MQ=255)
                                                                                               ttCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATcc                                        >  6:202095/1‑135 (MQ=255)
                                                                                               ttCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATc                                         >  2:165272/1‑134 (MQ=255)
                                                                                               ttCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAAGcc                                        >  1:226806/1‑135 (MQ=255)
                                                                                               ttCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCATCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATcc                                        <  5:99557/135‑1 (MQ=255)
                                                                                                 ccGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATCCAt                                      >  7:120381/1‑135 (MQ=255)
                                                                                                  cgcgCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATCCAt                                      <  1:243105/134‑1 (MQ=255)
                                                                                                            ggCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGATTCCTGAGACCGAGAAGGCCATGGCTGAATCCATCACCCGTAATTc                          >  3:280214/1‑136 (MQ=255)
                                                                                                            ggCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATCCATAACCCGTAATTc                          >  4:150226/1‑136 (MQ=255)
                                                                                                                      gACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATCCATCACCCGTAATTCCCCGTCgca                 >  1:337096/1‑133 (MQ=255)
                                                                                                                       aCTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATCCATCACCCGTAATTCCCCGTCGAAc                <  2:171005/135‑1 (MQ=255)
                                                                                                                         ttGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATCCATCACCCGTAATTCCCCGTCGAACGa              <  6:258171/135‑1 (MQ=255)
                                                                                                                                 gCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATCCATCACCCGTAATTCCCCGTCGAACGAGCGGGAtc      >  6:177129/1‑135 (MQ=255)
                                                                                                                                    gATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATCCATCACCCGTAATTCCCCGTCGAACGAGCGGGATCTCg   <  1:139055/135‑1 (MQ=255)
                                                                                                                                     aTCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATCCATCACCCGTAATTCCCCGTCGAACGAGCGGGATCTCGa  >  6:32039/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                     
GCTGGTCGATGCCGCCCACAACCCGGCATTAAGCGAGCTGTACCGTTACTTTTCCGGGGTCGTGGCTGCCGCTCTGCAACACAATATGGCCACCGTTCCGCGCTGCCAGGCCACGTTCGACTTGCATGGGCAGATCCTCGACGCCATCGAGCAACGTGACGCTGAACGGGCCAAGCGCCTGACCCGTATCCTCATTGAATCCTGAGACCGAGAAGGCCATGGCTGAATCCATCACCCGTAATTCCCCGTCGAACGAGCGGGATCTCGA  >  ACB122/1102833‑1103100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑