Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,157,983 A→C 28.9% S162A (TCG→GCG)  hexR_1 ← HTH‑type transcriptional regulator HexR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,157,9830AC28.9% 58.8 / 4.7 56S162A (TCG→GCG) hexR_1HTH‑type transcriptional regulator HexR
Reads supporting (aligned to +/- strand):  ref base A (19/18);  new base C (16/2);  total (36/20)
Fisher's exact test for biased strand distribution p-value = 7.62e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTTTCACGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACCTGCTGCGGGT  >  ACB122/1157851‑1158114
                                                                                                                                     |                                                                                                                                   
cGTTTTCACGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑acc                                                                                                                                   >  1:243799/1‑134 (MQ=255)
  ttttCACGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCTCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACgg                                                                                                                                >  8:383108/1‑136 (MQ=255)
  ttttCACGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑CCACgg                                                                                                                                >  8:52661/1‑136 (MQ=255)
  ttttCACGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACgg                                                                                                                                >  3:97800/1‑136 (MQ=255)
  tattCACGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCCGTGTAGGAAATAATCACGAACAGATCGCCGGTGTGGGCGCCCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑CCACg                                                                                                                                 >  2:176921/3‑135 (MQ=255)
               ggCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑CCCCGGCCAGGGTGAAGc                                                                                                                    >  1:39945/1‑135 (MQ=255)
               ggCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGc                                                                                                                    <  6:164562/135‑1 (MQ=255)
                 cgcgcCACTTCGACCAGTGCGCGGGGGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGGGGGGGCGACCGAGGCCAGCAGGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCgg                                                                                                                  <  5:154177/135‑1 (MQ=255)
                             aCCAGTTGGCGGGTGCGCGCGGTGTAGGAAATGATCACGAACCGATCGCCGGTGGGGGCGACCGAGGCCAGCATGCGCTGCTTCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGtgct                                                                                                     <  3:175235/136‑1 (MQ=255)
                                     gcgGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCGTGCGCTGCATCAGCACATCGGCGTGGGGCG‑CCACGGCCAGGGTGAAGCGGAAGAACATGTGCTGCGCATc                                                                                              >  6:280133/1‑135 (MQ=255)
                                     gcgGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑CCACGGCCAGGGTGAAGCGGCAGAACTTGTGCTGCGCATcc                                                                                             >  4:207643/1‑136 (MQ=255)
                                     gcgGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAAATTGTGGTGCGCagc                                                                                              >  6:282552/1‑133 (MQ=255)
                                     gcgGGGGCGCGCGGGGTCGCAAAGGCTCCCCAACAGCGCGCGGGGGGGGGCGACCGAGGCCAGCATGCGGGGCATCAGCACTTGGGGGGGGGCCC‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATcc                                                                                             <  8:158996/136‑1 (MQ=255)
                                          tGCGCCCGGTGTAGTAAATGATCACGAACAGAGCGCCGGGGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGc                                                                                        <  7:131426/136‑1 (MQ=255)
                                          tGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGc                                                                                        <  7:237485/136‑1 (MQ=255)
                                               ccGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACCCGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCg                                                                                   >  3:288524/1‑136 (MQ=255)
                                               ccGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCCTCCAGGGCCACCg                                                                                   >  3:357380/1‑136 (MQ=255)
                                               ccGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACATGTGCTGCGCATCCAGGGCCAcc                                                                                    >  2:50534/1‑135 (MQ=255)
                                                     tAGGAAATGAGCACGAACAGATCGCCGGGGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCt                                                                              <  4:21326/135‑1 (MQ=255)
                                                         aaaTGAGCACGACCCGCGCGCCGGTGTGGGCGACCGAGGCCAGCCTGCGCTGCAGCAGCACCTCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGgc                                                                         <  3:337368/136‑1 (MQ=255)
                                                              aTCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGGGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGgcgc                                                                       >  2:156904/1‑133 (MQ=255)
                                                               tCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑CCCCGGCCAGCTTGAATCGGAAGAACAAGTTGTGCCCATCCACGGCCACCGGGGCGGAGGCGCCGa                                                                    >  5:304139/1‑135 (MQ=255)
                                                                cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGGCGCCCCCGGCCCGG‑TGTAGCGGGCGAGCTTGTGGTGGGCCGCCCGGGGCCCCGGGGCGGGGGCGCCgcg                                                                   >  5:235074/1‑133 (MQ=255)
                                                                cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑CCCCGGCCAGGTTGAAGCCGCAGAACTTGTGGTGCGCATCCCGGGCCACCCGGGCTGAGGCGCCgcg                                                                   >  5:2170/1‑133 (MQ=255)
                                                                cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑CCACGGCCAGGTTGAAGCGGAAGAACTTTTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAgg                                                                  >  4:32619/1‑136 (MQ=255)
                                                                cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑CCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCCCCGGGGCTGCGGCGCCGAg                                                                   >  2:5698/1‑135 (MQ=255)
                                                                cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCCCCGGGGCTGAGGCGCCGAg                                                                   >  6:153774/1‑135 (MQ=255)
                                                                cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAg                                                                   >  1:27590/1‑135 (MQ=255)
                                                                cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCCGCCCATCCAGGGCCACCGGGGCTCGTGCGCCGAg                                                                   >  2:89109/1‑135 (MQ=255)
                                                                cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACATGTGCTGCGCATCCACGGCCACCGGGGCTGAGGCGCCGatg                                                                  >  7:309732/1‑134 (MQ=255)
                                                                cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCC‑CCCCCGCCACGTTGTACCGGAACAACTTGTGCTGCCCATACAGGGGCCCCGGGGCTGAGGCGCCGAgg                                                                  >  3:237497/1‑136 (MQ=255)
                                                                cACGAACAGATCGCCGGTGTGGGCGACCGAGGACAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAATAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGCgg                                                                  >  4:317532/1‑136 (MQ=255)
                                                                cACGAACAGAGCGCCGGTGGGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAgg                                                                  <  7:67903/136‑1 (MQ=255)
                                                                  cGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACCCGGCCAGGTTGAAGCGGAAGCACATGTGCTGCGCATCCCGGGCCACCGGGGCGGAGGCGCCGCGGc                                                                 >  1:280187/1‑135 (MQ=255)
                                                                      ccgctCGCCGGGGGGGGCGACCGAGGCCAGCAGGCGCGGCATCAGCACAGCGGGGGGGGCCG‑CCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCgaag                                                            <  3:99016/132‑1 (MQ=255)
                                                                               ggTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATc                                                   <  4:97800/136‑1 (MQ=255)
                                                                               ggTGGGGGCGACCGCGGCCAGCATGGGCTGCATCAGCACCGCGGCGGGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATc                                                   <  3:167682/136‑1 (MQ=255)
                                                                                    gggCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑CCCCGGCCCGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCCCCGGGGCTGAGGCGCCGCGGCCGCAGAAGTGGATCTGcc                                               >  1:30327/1‑135 (MQ=255)
                                                                                    gggCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACCCGGCCAGGGTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCg                                              >  3:369088/1‑136 (MQ=255)
                                                                                     ggCGCCCGAGCCCCGCAGCCGCTCCAACAGCCCCGCGGCGGGCGCCC‑ACCCGGCCAGGTTGACGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCg                                              <  1:176921/135‑1 (MQ=255)
                                                                                     ggCGACCGCGGCCAGCATGCGCGGCATCAGCACTGCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCg                                              <  2:289249/135‑1 (MQ=255)
                                                                                      gCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑CCACCGCCAGGTTGACGCGGAAGAACTTGTTCTGCGCATCCAGGGCCCCCGGGGCGGAGGCGCCGCGGCCCAATAAGGGGCTCTGGCgg                                             >  1:34854/1‑135 (MQ=255)
                                                                                           cGACGCCAGCCTGCGCCGCCTCCCCACAGCGGCGTGGGCCG‑ACACGGCCATGTTGAAGCGGAAGAACTAGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTgg                                       <  4:210775/136‑1 (MQ=255)
                                                                                             aGGCCAGCAGGCGCGGCACCAGCACATCGGCGGGGGCCG‑ACACGGCCAGGTGGAAGCGGAAGAACTTGGGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGat                                     <  8:309998/136‑1 (MQ=255)
                                                                                              ggCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGa                                      >  1:205310/1‑134 (MQ=255)
                                                                                                ccAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑TCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCCTCCAGGGCCCCCGGGGCGGAGGCGCCGAGGCCGCAGAAGTGGATCTGCCGGGCCTGGatca                                   >  1:45426/1‑135 (MQ=255)
                                                                                                       gcgcTGCATCAGCACATCGGCGTGGGCCG‑CCCCGGCCAGGTTGCAGCGGCAGAACTTGTGGTGCGCATCCAGGGCCACCGGGGCTGCGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCGGGCTCATCATGTc                            >  2:376953/1‑135 (MQ=255)
                                                                                                         gcggcagcagcaCTCGGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCGGAGGCTCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCa                          <  1:328583/132‑1 (MQ=255)
                                                                                                           tGCATCAGCACATCGGCGTGGGGCG‑CCACCGCCACGTTGAAGCGGAAGAACTTGTGGTGCGCATACAGGGCCCCCGGGGCGGAGGCGCCGAGGCCGAAGAAGTGGCTCTGCCCGGCCAGGATCATCATGTCCACg                        >  6:275542/1‑135 (MQ=255)
                                                                                                           tGCATCAGCACATCGGCGTGGGCCG‑CCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACgg                       >  4:334490/1‑136 (MQ=255)
                                                                                                               tCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGgcgc                    <  2:27590/135‑1 (MQ=255)
                                                                                                                cAGCACATCGGCGTGGGCCG‑ACACGGCCAGGGTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCCAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACcgcgcg                   >  2:377154/1‑135 (MQ=255)
                                                                                                                   cacaGCGGCGGGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCt                <  2:243799/135‑1 (MQ=255)
                                                                                                                         ggggTGGGCCG‑CCACGGCCAGGTTGAAGCGGAAGAACTTGGGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACctgc         <  3:107962/133‑1 (MQ=255)
                                                                                                                          gCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGGCGAAGAAGTGGATCTGCCGGGCCTGGATGATCATGTCCACGGCGCGGCTGACctgc         >  5:181919/1‑135 (MQ=255)
                                                                                                                           cGTGGGCCG‑ACACGGCCAGGGTGAAGCGGTAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCCTCATGTCCACGGCGCGGCTGCCctgctg       >  3:332732/1‑136 (MQ=255)
                                                                                                                                   g‑ACACGGCCAGGTTGAAGCGGAAGAAATTGTGCTGCGCATACAGGGCCACCGGGGCTGAGGCGCCGCGGCCGCAGAAGTGGATCTGCCGGGCCTTGATCATCATGTCCAAGGCGCGGCTGCCCTGCTGCGGGt  >  6:148129/1‑133 (MQ=255)
                                                                                                                                     |                                                                                                                                   
CGTTTTCACGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCG‑ACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACCTGCTGCGGGT  >  ACB122/1157851‑1158114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑