Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 52,849 A→T 6.5% K221* (AAA→TAA)  CJ019_00057 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB12252,8490AT 6.5% 184.3 / 4.3 62K221* (AAA→TAA) CJ019_00057hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/25);  new base T (2/2);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

GCGCTGTATCTGTCTTGCGACCACTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACATGCTGCACGTG  >  ACB122/52717‑52983
                                                                                                                                    |                                                                                                                                      
gcgcTGTATCTGTCTTGCGACCACTGGCTGCGCCATCGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGTGTGCCGTACTGTTCGGTGTCGCCGACTATCTGCCCGCGCTGATGAAGTACATCGAGaa                                                                                                                                       >  6:121130/1‑134 (MQ=255)
     gTATCTGTCTTGCGACCACTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGcc                                                                                                                                >  8:19881/1‑136 (MQ=255)
     gTATCTGTCTTGCGACCACTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGcc                                                                                                                                >  4:126492/1‑136 (MQ=255)
     gTATCTGTCTTGCGACCACTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGc                                                                                                                                 >  2:332726/1‑135 (MQ=255)
     gTATCTGTCTTGCGACCACTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGACGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGc                                                                                                                                 >  5:244230/1‑135 (MQ=255)
              ttGCGACCACTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCt                                                                                                                        >  4:342477/1‑135 (MQ=255)
                 cGACCACTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCaa                                                                                                                     >  6:24770/1‑135 (MQ=255)
                   aCCACTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGCCCTCAACtt                                                                                                                  >  4:29974/1‑136 (MQ=255)
                   aCCACTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGCCCTCAACtt                                                                                                                  >  4:318390/1‑136 (MQ=255)
                   aCCACTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGCCCTCAACt                                                                                                                   >  6:317958/1‑135 (MQ=255)
                   aCCACTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACt                                                                                                                   >  6:134669/1‑135 (MQ=255)
                   aCCACTGGCTGCGCCATGCCCAATTCGGGGCGGTGAAAGCCAGCTTCTACAAAGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACtt                                                                                                                  >  3:92189/1‑136 (MQ=255)
                      aCTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTtctc                                                                                                               <  8:129809/136‑1 (MQ=255)
                      aCTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTtctc                                                                                                               <  4:174302/136‑1 (MQ=255)
                       cTGGCTGCGCCATGGCCATTTGGGGGAGGTGAAAGCCAGCTGCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTtctc                                                                                                               <  6:267617/135‑1 (MQ=255)
                        tGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTcc                                                                                                              <  1:241275/135‑1 (MQ=255)
                         ggCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTccc                                                                                                             >  1:6330/1‑135 (MQ=255)
                           cTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTccc                                                                                                             >  6:150685/1‑133 (MQ=255)
                             gcgcCATGGCCATTTGGGGGCGGTGAAAGCAAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAg                                                                                                         >  2:225271/1‑135 (MQ=255)
                              cgcCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGt                                                                                                       <  3:126492/136‑1 (MQ=255)
                               gcCATGGCGATTTGGGGGCTGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCTCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGt                                                                                                       <  1:151865/135‑1 (MQ=255)
                               gcCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGt                                                                                                       <  1:332726/135‑1 (MQ=255)
                                       cATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAg                                                                                              >  3:113054/1‑136 (MQ=255)
                                        atgtGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTAATGTTCGGGGGCGCCGACTATGGGCCCGCGCTGATGAAGTACAACGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGt                                                                                             <  3:246210/133‑1 (MQ=255)
                                              gggCGGTTAAAGCCAGCTTCTACAATGCGGGGGCCGTACTGTTGGGTGTCGCCGAGTTTGTGCCCGCGCTGATGAAGTAAATCGAGAAATACGCCGGCGACCTCAACTTCTCCCACTGGTTGGTAGCAGTAGAc                                                                                         <  6:325932/134‑1 (MQ=255)
                                                    tGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCg                                                                                   >  2:363966/1‑134 (MQ=255)
                                                          ccAGATTCTACAATGCGGGCGCCGTACTGTGCGGTGGCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCCCAGGTTGGTAGCAGTAGACGGCCCGGGAAAgc                                                                            <  5:218950/135‑1 (MQ=255)
                                                                 cTACAATGGGGGGGCCGTACTGTTCGGTGGGGCCGACTATGTGCCCGCTCTGATTATTTACATCTATAAATACGCCGCCCACCTCAACTTCTCCCACCGGTTGGTAGCAGTAGACGGCCCGGGAAAGAGCGCTAc                                                                     <  8:141459/135‑1 (MQ=255)
                                                                 cTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACa                                                                    >  8:69124/1‑136 (MQ=255)
                                                                 cTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACa                                                                    >  8:284107/1‑136 (MQ=255)
                                                                 cTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACa                                                                    >  4:163485/1‑136 (MQ=255)
                                                                  tccAATGGGGGTGCGGTACTGTTCGGTGGCGCCGACTATGGGCCCGCTCTTATGAAGTACATCTAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTAc                                                                     <  5:264690/132‑1 (MQ=255)
                                                                  tACACTGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGACAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTAc                                                                     <  2:210898/134‑1 (MQ=255)
                                                                  tACACTGCGGGTGCCGTACTGTTCGGTGGCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTAc                                                                     <  2:52587/134‑1 (MQ=255)
                                                                          gggTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGc                                                             >  6:298536/1‑134 (MQ=255)
                                                                          gggTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCg                                                            >  4:375375/1‑135 (MQ=255)
                                                                          gggTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGACCGGGAAAGCGCGCTACATTCTTGc                                                             >  1:265175/1‑134 (MQ=255)
                                                                          gggTGCCGTACAGTTCGGAGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCAGTCGACCTCATCTTCTCCCACAGTTAGGTAGCAGTAGACGGCCCTCGAAAGCGAGCTACATTCTTGc                                                             >  6:29087/1‑134 (MQ=255)
                                                                               ccGTACTGTTCGGTGTCGCCGACTACGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTAccc                                                       >  1:117401/1‑135 (MQ=255)
                                                                                    cTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGcc                                                                                                                                >  8:149221/1‑57 (MQ=255)
                                                                                    cTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGcc                                                                                                                                <  7:149221/57‑1 (MQ=255)
                                                                                          ggTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGTAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCg                                                                                   >  4:373814/1‑96 (MQ=255)
                                                                                          ggTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGTAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCg                                                                                   >  4:380100/1‑96 (MQ=255)
                                                                                          ggTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGTAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCg                                                                                   <  3:373814/96‑1 (MQ=255)
                                                                                          ggTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGTAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCg                                                                                   <  3:380100/96‑1 (MQ=255)
                                                                                               cGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGcagcag                                       >  2:143480/1‑135 (MQ=255)
                                                                                               cGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCagcagc                                      >  7:395307/1‑136 (MQ=255)
                                                                                                 ccGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCg                                     <  2:290108/135‑1 (MQ=255)
                                                                                                 ccGACTATGTGCCCGCGCGGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCagcagc                                      >  1:290108/1‑134 (MQ=255)
                                                                                                   gACTATGTGCCCGCGCTGATGAAGAACATCCATAAAACCCCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTCGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCGGCAGCGaa                                   <  1:363966/135‑1 (MQ=255)
                                                                                                    aCTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTCCCCTGCCCGATGGCAGCAGCGaaa                                  >  5:99731/1‑135 (MQ=255)
                                                                                                         gtgCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTAGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCACGATGGCAGCAGCGAACCgcgc                             >  1:208547/1‑135 (MQ=255)
                                                                                                            cccGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATc                          <  7:69124/135‑1 (MQ=255)
                                                                                                                  cTGATGAAGTACATCGAGAAATACGCCGTGGACCTCAACTTCTCCCACTGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCt                   <  4:224838/136‑1 (MQ=255)
                                                                                                                   tgatgaAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCt                   >  1:258005/1‑135 (MQ=255)
                                                                                                                     ttgaAGTACATCGAGAACTACCCCGTCGACCTCAACTTCTCCCACAGGTGGGTAGCAGTAGACGGCCCGGGAAAGCTCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCg                <  7:387063/135‑1 (MQ=255)
                                                                                                                        aaGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCACGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGAc              >  6:310584/1‑135 (MQ=255)
                                                                                                                         aGTACATCGAGAAATCCGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACAt            <  8:199851/136‑1 (MQ=255)
                                                                                                                          ttACATGGATAAATACGCCGTCCCACTAAACTTCTCCCAAAGGTTGGTATCATGAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACAt            <  5:150685/134‑1 (MQ=255)
                                                                                                                             cATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGaa                                   >  2:3126/1‑109 (MQ=255)
                                                                                                                             cATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGaa                                   <  1:3126/109‑1 (MQ=255)
                                                                                                                              aTCTAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACAtgctgc       <  3:288401/136‑1 (MQ=255)
                                                                                                                                cGAGATATACCCCGTCGACCTCAAGTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTAGCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGTCATGCTGCa      <  2:117401/135‑1 (MQ=255)
                                                                                                                                 gagaAATACGCCGGCGACCTAAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACATGCTGCa      <  3:318390/134‑1 (MQ=255)
                                                                                                                                  agaAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACCTTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCGTCGACATGCTGCACg    >  5:240548/1‑135 (MQ=255)
                                                                                                                                  agaAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCTACATGCTGCACg    >  5:180226/1‑135 (MQ=255)
                                                                                                                                  agaAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACATGCTGCACg    >  6:160718/1‑135 (MQ=255)
                                                                                                                                  agaAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACATGCTGCACGt   >  4:353171/1‑136 (MQ=255)
                                                                                                                                  agaAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACATGCTGCACGt   >  4:177100/1‑136 (MQ=255)
                                                                                                                                   gaAATCCGCCGGCGCCCTCAACTTCTCCCACAGGTTGTTAGCAGAAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACATGCTGCACGt   <  6:240548/135‑1 (MQ=255)
                                                                                                                                   caaaTACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACATGCTGCACGTg  <  3:282143/135‑1 (MQ=255)
                                                                                                                                    aaaTCCCCCGTCGACCTAAACTCCTCCCACTGTTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACATGCTGCACGTg  <  5:317958/135‑1 (MQ=255)
                                                                                                                                    aaaTACGCCGTCGACCTCAACTTCTCCCACCGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACATGCTGCACGTg  <  5:374952/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
GCGCTGTATCTGTCTTGCGACCACTGGCTGCGCCATGGCCATTTGGGGGCGGTGAAAGCCAGCTTCTACAATGCGGGTGCCGTACTGTTCGGTGTCGCCGACTATGTGCCCGCGCTGATGAAGTACATCGAGAAATACGCCGTCGACCTCAACTTCTCCCACAGGTTGGTAGCAGTAGACGGCCCGGGAAAGCGCGCTACATTCTTGCGTACCCTGCCCGATGGCAGCAGCGAAACGCGCATCGAGGCCTTCGACATGCTGCACGTG  >  ACB122/52717‑52983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑