Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,167,947 C→A 6.8% intergenic (+16/‑32) CJ019_01101 → / → CJ019_01102 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,167,9470CA 6.8% 188.9 / 4.3 59intergenic (+16/‑32)CJ019_01101/CJ019_01102hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/33);  new base A (2/2);  total (24/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

TCTGTGGGTAGGGCGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTGCCCGCGCCATCCTGCGCTGGCTGGTGCGCGAAAAGGTCGTCG  >  ACB122/1167814‑1168081
                                                                                                                                     |                                                                                                                                      
tCTGTGGGTAGGGCGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCAt                                                                                                                                      >  8:36944/1‑136 (MQ=255)
tCTGTGGGTAGGGCGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGGGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCAt                                                                                                                                      <  4:31096/136‑1 (MQ=255)
    tgGGTAGGGCGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTTGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTAcc                                                                                                                                  <  8:279806/136‑1 (MQ=255)
    tgGGTAGGGCGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTAc                                                                                                                                   <  4:358929/135‑1 (MQ=255)
     gggTAGGGCGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCg                                                                                                                                 >  3:111947/1‑136 (MQ=255)
      ggTAGGGCGTCGGTTGACCAACCGGTTGTCGCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCg                                                                                                                                 <  2:364881/135‑1 (MQ=255)
      ggTAGGGCGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTAGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCg                                                                                                                                 <  2:127174/135‑1 (MQ=255)
          gggCGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGtt                                                                                                                            <  3:73074/136‑1 (MQ=255)
           ggCGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGCTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGtt                                                                                                                            <  1:372448/135‑1 (MQ=255)
            gCGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCg                                                                                                                                 <  4:147932/129‑1 (MQ=255)
            gCGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCg                                                                                                                                 >  3:147932/1‑129 (MQ=255)
            gCGTCCGTTGACCAACCTGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGATGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTtc                                                                                                                          >  7:71279/1‑136 (MQ=255)
             cggcGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTtc                                                                                                                          <  6:38943/132‑1 (MQ=255)
             cGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTtct                                                                                                                         >  3:280383/1‑136 (MQ=255)
                 ggTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAgg                                                                                                                     >  7:191178/1‑136 (MQ=255)
                 ggTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAg                                                                                                                      >  6:310386/1‑135 (MQ=255)
                 ggTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTAATTACCGGCGtt                                                                                                                            <  7:209144/129‑1 (MQ=255)
                 ggTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTAATTACCGGCGtt                                                                                                                            >  8:203576/1‑129 (MQ=255)
                 ggTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTAATTACCGGCGtt                                                                                                                            <  7:203576/129‑1 (MQ=255)
                 ggTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTAATTACCGGCGtt                                                                                                                            >  8:209144/1‑129 (MQ=255)
                   ttGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCt                                                                                                                   >  7:358267/1‑136 (MQ=255)
                      aCCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGGGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCtt                                                                                                                  <  6:180334/134‑1 (MQ=255)
                       ccAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTc                                                                                                               <  8:358267/136‑1 (MQ=255)
                         aaCCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCg                                                                                                                                 >  7:212734/1‑116 (MQ=255)
                         aaCCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCg                                                                                                                                 <  8:212734/116‑1 (MQ=255)
                               ttGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCAt                                                                                                       <  4:250485/136‑1 (MQ=255)
                               ttGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCAt                                                                                                       <  3:142484/136‑1 (MQ=255)
                                    ccGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGTCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAac                                                                                                   <  1:116024/135‑1 (MQ=255)
                                     cgcgAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAacac                                                                                                 <  3:344860/136‑1 (MQ=255)
                                         aaGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAacac                                                                                                 <  4:219764/132‑1 (MQ=255)
                                         aaGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAacac                                                                                                 >  3:219764/1‑132 (MQ=255)
                                              ttCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGcat                                                                                        <  4:280383/136‑1 (MQ=255)
                                               tCGTACGGCTGGTTACCTGGCTGGTTCTGGCCAGTGGTCTGGCTTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGcat                                                                                        <  8:175975/135‑1 (MQ=255)
                                               tCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGcat                                                                                        >  4:148652/1‑135 (MQ=255)
                                                     ggCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGtt                                                                                 <  3:132536/136‑1 (MQ=255)
                                                     ggCTGGTGAACTGGCTGGTGCTGGACAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTGTCAGGCTTGTCCCGTTCATGATCACCCAGAGCATCATCGtt                                                                                 <  7:359528/136‑1 (MQ=255)
                                                         ggTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTcc                                                                               >  6:200478/1‑134 (MQ=255)
                                                          gTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGGTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCaa                                                                             <  6:81243/135‑1 (MQ=255)
                                                             aCCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGc                                                                          >  6:129787/1‑135 (MQ=255)
                                                                         cTGGCCAGTGGGCTGGCGTTGAGTGGTCGCTACCTGAGCGCCTGAGCGGTAGAACTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTcc                                                              <  5:90306/135‑1 (MQ=255)
                                                                            gCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGt                                                          >  4:112617/1‑136 (MQ=255)
                                                                               aGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGcat                                                                                        >  4:347709/1‑103 (MQ=255)
                                                                               aGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGcat                                                                                        <  3:347709/103‑1 (MQ=255)
                                                                               aGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTACCAAAGCTGTCTACCGTCCCCGTGCa                                                       >  4:287956/1‑136 (MQ=255)
                                                                                         cGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCg                                                                                                                                 <  8:218033/52‑1 (MQ=255)
                                                                                         cGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCg                                                                                                                                 >  7:218033/1‑52 (MQ=255)
                                                                                           ttGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTg                                            >  1:265525/1‑135 (MQ=255)
                                                                                              aTTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTGccc                                         <  4:148718/135‑1 (MQ=255)
                                                                                              aTTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTGccc                                         <  4:111947/135‑1 (MQ=255)
                                                                                               ttGGTCGCTACCTGAGCGCCTGAGCTGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTGccc                                         <  5:393106/134‑1 (MQ=255)
                                                                                                   tCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTGCCCGCGcc                                    <  5:88700/135‑1 (MQ=255)
                                                                                                   tCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTGCCCGCGcc                                    <  5:200478/135‑1 (MQ=255)
                                                                                                        aCCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGGTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTGCCCGCGCCATCCt                               <  6:58950/135‑1 (MQ=255)
                                                                                                           tgagcgcctgagcgGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAACCTGTCTACCGTCCCGGGGCATGAAGCCAGTGCCCGCGCCCTCCTgcg                            >  5:22100/1‑135 (MQ=255)
                                                                                                                    gagcgGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTGCCCGCGCCATCCTGCGCTGGCTGGTg                  >  4:318678/1‑136 (MQ=255)
                                                                                                                         gTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTGCCCGCGCCATCCTGCGCTGGCTGGTGCGCGa             <  3:218786/136‑1 (MQ=255)
                                                                                                                             aaTTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTGCCCGCGCCATCCTGCGCTGGCTGGTGCGCGaaaa          >  5:144958/1‑135 (MQ=255)
                                                                                                                                  cGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATAGTTACAAAGCAGTCTACCGTCCCGGTGCATGAAGCCCGTGCCCGCGCCAACCTGCGCTGGCTGGTGCGCGAAAAGgtcgt    >  7:368383/1‑136 (MQ=255)
                                                                                                                                    tCATTACCGGCGTGTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTGCCCGCGCCATCCTGCGCTGGCTGGTGCGCGAAAAGGtcgtcg  <  3:214453/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TCTGTGGGTAGGGCGTCGGTTGACCAACCGGTTGTCCCGCGAAGCGTTCGTACGGCTGGTGACCTGGCTGGTGCTGGCCAGTGGGCTGGCGTTGATTGGTCGCTACCTGAGCGCCTGAGCGGTAGAATTGCGTCATTACCGGCGTTTCTCAGGCTTGTCCCGTTCATGAACACCCAGAGCATCATCGTTCCAAAGCTGTCTACCGTCCCGGTGCATGAAGCCCGTGCCCGCGCCATCCTGCGCTGGCTGGTGCGCGAAAAGGTCGTCG  >  ACB122/1167814‑1168081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑