Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,179,510 C→T 87.9% S554S (AGC→AGT)  purL → Phosphoribosylformylglycinamidine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,179,5100CT87.9% 111.2 / 6.0 41S554S (AGC→AGT) purLPhosphoribosylformylglycinamidine synthase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base T (20/16);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCGGCAACACCCCGGTGGA  >  ACB122/1179387‑1179638
                                                                                                                           |                                                                                                                                
ggTCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                      <  3:35835/136‑1 (MQ=255)
ggTCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTc                                                                                                                      <  4:175843/136‑1 (MQ=255)
 gTCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                      <  5:59495/135‑1 (MQ=255)
 gTCAACGACGGGGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                      <  2:199215/135‑1 (MQ=255)
 gTCAAAGCCGGTGGCCGCGGTGGTCGCGTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                      <  3:54353/135‑1 (MQ=255)
  tCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                      <  1:255279/134‑1 (MQ=255)
    aaCGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAgc                                                                                                                  >  3:72936/1‑136 (MQ=255)
    aaCGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGCCTTCGAg                                                                                                                   >  6:121328/1‑135 (MQ=255)
     acgacgGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTCGAgc                                                                                                                  <  1:185514/135‑1 (MQ=255)
         cgGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCtt                                                                                                              >  4:254071/1‑135 (MQ=255)
             ggCCGCGGTGGTCGCTTCTAGCTGAGCAACGTGCCCAACGACGAGACGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGAGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAg                                                                                                          <  2:164342/135‑1 (MQ=255)
              gCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAgg                                                                                                         >  1:210348/1‑135 (MQ=255)
              gCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAgg                                                                                                         >  2:353103/1‑135 (MQ=255)
              gCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGGCAGTGCGGTCGACGTCGCGCGCGTCAAGGc                                                                                                        >  8:271246/1‑136 (MQ=255)
                 gcgGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACGTCGAGCGCTTCAAGGccc                                                                                                      >  1:73250/1‑134 (MQ=255)
                                 gCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCGCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACg                                                                                      <  1:106786/135‑1 (MQ=255)
                                        aaCGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                      <  2:126242/96‑1 (MQ=255)
                                        aaCGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                      >  1:126242/1‑96 (MQ=255)
                                        aaCGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCg                                                                               >  1:273575/1‑135 (MQ=255)
                                        aaCGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGt                                                                              >  3:32529/1‑136 (MQ=255)
                                           gggCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGttt                                                                            <  1:301366/133‑1 (MQ=255)
                                             gCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGCGCGCGAACGTTGCCCGTTTGcc                                                                         >  7:242296/1‑136 (MQ=255)
                                             gCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGcc                                                                         >  3:337884/1‑136 (MQ=255)
                                              cccATCGAAGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACTAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGcc                                                                         <  8:384144/135‑1 (MQ=255)
                                              cccAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGcc                                                                         <  3:254071/135‑1 (MQ=255)
                                                        aGCCGGGCATGGCCCCGCACGAAATCTTTAGAAACTAATAGCAATAGAGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTggtgggtg                                                               >  4:28137/1‑135 (MQ=255)
                                                         gCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGa                                                              <  1:2540/135‑1 (MQ=255)
                                                           cGGGCATGGCCCCGTACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAAAGTTGCCCGTTTGCCGGGGTGGGTGAAg                                                            >  1:185933/1‑135 (MQ=255)
                                                           cGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGCACGTTGCCCGTTTGCCGTGGTGGGTGAAg                                                            >  6:191854/1‑135 (MQ=255)
                                                                     cccGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTGAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTgaaga                                                  <  5:260170/135‑1 (MQ=255)
                                                                          aCGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGc                                             >  6:280704/1‑135 (MQ=255)
                                                                           cGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCAc                                           >  3:124538/1‑136 (MQ=255)
                                                                               aTCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGCACGTTGCCCGTTTGCCATGGTGGGTGAAGCGACTGAAGAACAGCACCTg                                        >  1:252686/1‑135 (MQ=255)
                                                                                 cTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGAcc                                     >  3:148100/1‑136 (MQ=255)
                                                                                                     agCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCgg                 <  8:76069/136‑1 (MQ=255)
                                                                                                       cGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGCGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCCTCAGCGACAGCCACATCgg                 >  5:323655/1‑134 (MQ=255)
                                                                                                          tACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCGGCAaca            >  3:359809/1‑136 (MQ=255)
                                                                                                                  ggCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCGGCAACACCCCGGTg    <  7:216706/136‑1 (MQ=255)
                                                                                                                   gCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCCCCTGACCGTCAGCGACAGCCACTTCGGCAACACCCCGGTg    >  6:338448/1‑135 (MQ=255)
                                                                                                                   gCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCGGCAACACCCCGGTgg   <  3:136474/136‑1 (MQ=255)
                                                                                                                    ccGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCGGCAACACCCCGGTGGa  <  3:28137/136‑1 (MQ=255)
                                                                                                                           |                                                                                                                                
GGTCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCGGCAACACCCCGGTGGA  >  ACB122/1179387‑1179638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑