Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,213,098 C→T 8.1% A421T (GCC→ACC)  dctD_2 ← C4‑dicarboxylate transport transcriptionalregulatory protein DctD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,213,0980CT 8.1% 155.8 / 3.5 52A421T (GCC→ACC) dctD_2C4‑dicarboxylate transport transcriptionalregulatory protein DctD
Reads supporting (aligned to +/- strand):  ref base C (27/20);  new base T (2/2);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTGGGGCCTTTCTTGAACGCTGGCAGGC  >  ACB122/1212966‑1213229
                                                                                                                                    |                                                                                                                                   
accacGCCTGCGCCCAGGGCCGCGTTGAATTCCAGGTCCCCGTTGGTTTCCCGGTTGGGGTGAGTCAAGCGAGGCCGTTTTTTTTCACGTTGTGGCACCGCGGGGGCTTGGCCCAGCCCCGCTCCGGGggggccgg                                                                                                                                  >  4:314135/1‑134 (MQ=255)
 ccacGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGctggcctgg                                                                                                                                 <  8:68755/136‑1 (MQ=255)
       cTGCGCCCAGGGCCGCTTTGAATACCAGGTCCCCGTTGTTTTCCAGTTTGGGTTCAGTCCGGCGAGGCCGGATTTTTTAACTTTGTCGGACAGCGTGGGCTTTGCCATTCCCCGCTCCAGGCGGGTCTGGCGCAgg                                                                                                                           >  4:286558/1‑136 (MQ=255)
       cTGCGCCCAGGGCCGCGTTGAATAACAGGTCCACGTTGTTTTCCAGTTTGGGTTGAGTCAGGCGAGGCCGGATTTTTTAACTTTTTAGTACAGCGTGGTCTTTGCCATTCCCAGCTCCTGGCTGGTCTGGCTCAgg                                                                                                                           >  4:281486/1‑136 (MQ=255)
            cccAGGGCCGCGTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGGATTTTTTCACTTTGTCGAACAGCGTGGTCTTTGCCATTCCCAGCTCCGGGCTGGCCTGGCTCCGGTTGcc                                                                                                                      >  8:101797/1‑136 (MQ=255)
            cccAGCGCCGCTTTGAATACCAGGGCCACGTTGTTTTCCAGTATGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGc                                                                                                                       <  1:89911/135‑1 (MQ=255)
               aGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTGCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTGACGTTGTCGCACAGCGTGGTCTTGGCCCTGCCCAGCTCCGGGCTGGGCTGGCTCCGGTTGCCAc                                                                                                                    >  2:31623/1‑135 (MQ=255)
                gggCCGCTTTGAATACCAGGTCGACGTTGTTATCCAGTTTGGGTTCAGTCCGGCGAGGCCGTATTTTTTCACTTTGTCTAACAGCGTCGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCAcc                                                                                                                   <  6:173424/135‑1 (MQ=255)
                    cGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTg                                                                                                               >  6:131934/1‑135 (MQ=255)
                         tGAATACCAGGTCCACGTTGTTTTGCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCAGGCGGGCCTGGCTCAGGTTGCCACCCGTGCGTTGc                                                                                                         >  8:36575/1‑136 (MQ=255)
                         tGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGtt                                                                                                           <  7:195237/134‑1 (MQ=255)
                         tGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGtt                                                                                                           <  7:100690/134‑1 (MQ=255)
                         tGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTAACATTGTAGAACAGCGTGGTCTTTGCCAAGCCCAGCTCCTGGCTGGCCTGGCTCACGTTGCCCCCCGTGCGTTGc                                                                                                         >  8:180349/1‑136 (MQ=255)
                         tGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTAAATTTGTCGAACAGCGTGGTCTTTTCCATGCCCAGCTCCAGGCTGGCCTGGCTCAGGTTGCCACCCCTGCGTTg                                                                                                          >  8:75297/1‑135 (MQ=255)
                         tGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGGTCAGTCAGGCGACGCCCGATTTTTTCACTTTGTCGAACACCGTGGTCTTGGCCAAGCCCAGCTACAGGCTGGCCTGGCTACGGTTGCCAACCCTGGCTTGc                                                                                                         >  4:248963/1‑136 (MQ=255)
                         tGAATACCACGTACCCCTTTTTTTACACTTTGGGTTCAGTCAGGCGACGCCGTATTTTTTGACTTTGTCGAACAGCGTGGTCTTTGCAATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTTCCACCCGTGCGTTg                                                                                                          >  6:93193/1‑135 (MQ=255)
                          gAATACCAGGTTCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGtt                                                                                                           <  5:139089/133‑1 (MQ=255)
                           aaTACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCa                                                                                                        <  7:180349/135‑1 (MQ=255)
                            aTACCAGGTCCACGGTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAgg                                                                                                      <  7:325807/136‑1 (MQ=255)
                               ccAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGc                                                                                                    <  7:75297/135‑1 (MQ=255)
                                   gTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCa                                                                                                >  1:223579/1‑135 (MQ=255)
                                     ccACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGt                                                                                              <  5:375830/135‑1 (MQ=255)
                                     ccACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGt                                                                                              <  5:247537/135‑1 (MQ=255)
                                      cACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGtt                                                                                                           >  4:270092/1‑121 (MQ=255)
                                      cACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGtt                                                                                                           <  3:270092/121‑1 (MQ=255)
                                       aCGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTg                                                                                             <  2:305403/134‑1 (MQ=255)
                                           tgttTTCCAGTTTGGGTTAATTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAAAATCGTTGTCTTGGCCAAGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGGCACCCGTGCCTTGCAGGGCATCAGTGAGctg                                                                                        >  6:273910/1‑133 (MQ=255)
                                             ttttCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAgg                                                                                       <  5:131934/134‑1 (MQ=255)
                                             ttttCCAGTTTGGGGTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAgg                                                                                       <  5:214775/134‑1 (MQ=255)
                                                 ccAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTAACTTTTTCGAACAGCGTGGTCTTTGCCAAGCCCAGATCCTGGCAGGCCTGGCTCACGTTGCCACCCGTGCGTTGCAGGGCCTCAGTGAGCAGGTTGCg                                                                                  >  6:259419/1‑135 (MQ=255)
                                                      ttGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGa                                                                             <  1:387606/135‑1 (MQ=255)
                                                        gggTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATg                                                                          >  8:180094/1‑136 (MQ=255)
                                                          gTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGc                                                                         >  1:67714/1‑135 (MQ=255)
                                                             cagtcagGCGAGGCCGGATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCCGGTTGCCCCCCGGGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTcc                                                                     >  4:5899/1‑136 (MQ=255)
                                                                  agGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGTCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCg                                                                 <  3:286558/135‑1 (MQ=255)
                                                                  agGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGTCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCg                                                                 <  3:281486/135‑1 (MQ=255)
                                                                       aGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCgg                                                           >  3:340684/1‑136 (MQ=255)
                                                                                     cACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGcc                                              <  7:180094/135‑1 (MQ=255)
                                                                                               aaCAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGca                                    <  2:67714/135‑1 (MQ=255)
                                                                                                       ggTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTg                           >  4:376245/1‑136 (MQ=255)
                                                                                                       ggTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGAGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCt                            >  2:224471/1‑135 (MQ=255)
                                                                                                         tCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCGGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGACATGGTTGGCACCACCGCTggg                         >  8:94632/1‑136 (MQ=255)
                                                                                                               ccATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTGGGGCCtt                    <  2:115427/135‑1 (MQ=255)
                                                                                                                 aTGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTGGGGCCTTTCt                 >  3:295159/1‑136 (MQ=255)
                                                                                                                    cccAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTGGGGCCTTTCTTGa              <  3:376245/136‑1 (MQ=255)
                                                                                                                     ccAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCAt                                                                                                  <  2:138959/51‑1 (MQ=255)
                                                                                                                     ccAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCAt                                                                                                  >  1:138959/1‑51 (MQ=255)
                                                                                                                      cAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCCCCGCTGGGGCCTTTCTTGaa             >  5:107069/1‑135 (MQ=255)
                                                                                                                      cAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTGGGGCCTTTCTTGaa             >  1:309101/1‑135 (MQ=255)
                                                                                                                      cAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTGGGGCCTTTCTTGaa             >  1:244963/1‑135 (MQ=255)
                                                                                                                      cAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTGGGGCCTTTCTTGAAc            >  4:266802/1‑136 (MQ=255)
                                                                                                                      cAGCTCCTGGCTGGCCTGGCGCAGGTTGCCACCCGTGCGTTGCAGGGCCTCAGTGAGCAGGTGGCGTTCGAATGCTGCCCCCGCCTCGGCTCAACGCAGGCCGTGGTTGGCCCCACCGCTGGGGCCTTTCTTgcc             >  2:66475/1‑133 (MQ=255)
                                                                                                                          tCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTGGGGCCTTTCTTGAACGCTg        >  4:359469/1‑136 (MQ=255)
                                                                                                                            ctggctggCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTGGGGCCTTTCTTGAACGCTGGc      >  8:87069/1‑136 (MQ=255)
                                                                                                                               gctggcCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTGGGGCCTTTCTTGAACGCTGGCAgg   <  3:266802/136‑1 (MQ=255)
                                                                                                                                ctggcctggcTCAGGGTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGGTGCGGTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTGGGGCCTTTCTTGAACGCTGGCAgg   <  5:259419/135‑1 (MQ=255)
                                                                                                                                 tggcctggcTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCCCCGCTGGGGCCTTTCTTGAACGCTGGCAGGc  >  1:266890/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                   
ACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTGGCCATGCCCAGCTCCTGGCTGGCCTGGCTCAGGTTGCCACCCGTGCGTTGCAGGGCATCAGTGAGCAGGTTGCGTTCGAATGCTTCCACCGCCTCGGCAAAAAGCAGGCCTTGGTTGGCACCACCGCTGGGGCCTTTCTTGAACGCTGGCAGGC  >  ACB122/1212966‑1213229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑