Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,339,139	T→G	19.7%	*269C (TGA→TGC)	udh ←	Uronate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,339,139	0	T	G	19.7%	86.6 / 3.9	57	*269C (TGA→TGC)	udh	Uronate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (23/21);  new base G (9/4);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 3.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTGGCGAGCGGGAATGTCTACCCAGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGTACATAGCCCAGGT  >  ACB122/1339010‑1339272                                                                                                                                  |                                                                                                                                      aaTTGGCGAGCGGGAATGTCTACCCAGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGtt                                                                                                                                  <  2:304545/135‑1 (MQ=255)         aGCGGGAATGTCTACCCAGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGggcc                                                                                                                         <  7:180515/136‑1 (MQ=255)              gAATGTCTACCCAGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGGCGCCGGGGGTGGGGGGCGTTGGAAGggccggcc                                                                                                                     >  1:393791/1‑135 (MQ=255)                        ccAGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCTAAAg                                                                                                           <  6:14663/135‑1 (MQ=255)                         cAGTACAGGGCCTGTGCACCGCGGGGCCACAGAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGGGCCTCCTGGCGTGGGGTCAGTTGACCGGGCCGGCCACGGCGAAAgcg                                                                                                         <  8:267573/136‑1 (MQ=255)                          aGTACCGGGCCTGTGCACCGGGGGGCCACACAGGGCTTGCGCCGGAACCGTGGCGGGCGGCCACTATCAGTTCGCCGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGACCGGGCCGGCCACGGCGAAAgcg                                                                                                         <  2:187529/135‑1 (MQ=255)                               aGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGCCGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGccc                                                                                                   >  3:172284/1‑136 (MQ=255)                               aGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCCCGGCGAAAGCgccgcc                                                                                                    >  6:238095/1‑135 (MQ=255)                               aGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAgcg                                                                                                         <  5:79280/130‑1 (MQ=255)                               aGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAgcg                                                                                                         >  6:79280/1‑130 (MQ=255)                               aGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGGCAGTTGCACGGGCCGGCCACGGCGCAAGCgccgcc                                                                                                    >  6:208827/1‑135 (MQ=255)                               aGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGGGGGGGGCGTTGAACGGGCCGGCCCCCGCGAAAGCgccccc                                                                                                    >  2:158409/1‑135 (MQ=255)                               aGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTAGCAGTTCATGGCCGTGCCTCCGGGCGGGGGGGGAGGTTGACGGGCCGGCCCCCGCGCAAGCcccgcc                                                                                                    >  5:37791/1‑135 (MQ=255)                                    cTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTgg                                                                                                >  7:245439/1‑134 (MQ=255)                                    cTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTgg                                                                                                >  3:263812/1‑134 (MQ=255)                                    cTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCGCCTGGGGGGGGGGGCGTTGTTCGGGCCGGCCCCCGCGCAAGCGCCGcccag                                                                                                 >  1:187529/1‑131 (MQ=255)                                         cACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGCTCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCCTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAACCGCCGCCCTGGTAGAcc                                                                                          >  2:217221/1‑135 (MQ=255)                                         cACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGAcc                                                                                          >  6:301449/1‑135 (MQ=255)                                         cACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGGGGGGGGAGTTGAACGGGCCCGGCACGGCGCAAGCGCCGCCCggggtga                                                                                            >  5:23751/1‑129 (MQ=255)                                         cACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGAAGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGGGATTTGAACGGGCCGGCCAAGGCGACAGCGCCGCCCGGGTAGACCa                                                                                         >  3:252147/1‑136 (MQ=255)                                               ggTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGCCGATCAGTTCGCAGTTCATGGCCGTGCCGCCTGGCGGGGGGGGAGTTGTACGCGCCCGCCACCGCGCAAGCGCCGCCCGGGTAGGCCATGcgcg                                                                                   >  3:227734/1‑136 (MQ=255)                                               ggTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCCGCCCCGGCGAAAGCGCCGCCCTGGTAGACCATGCTCg                                                                                   >  8:257822/1‑136 (MQ=255)                                                    cacacaGGGCTTTCGCCGGTACCGTTGCGGGCGTCGCCGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCCCCGCGAAAGCGCCGCCCGGGTAGACCCTGCTCgggg                                                                                >  6:125069/1‑133 (MQ=255)                                                    cacacaGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGGGGGGGGTGAGTTGCACCGGCCGGCCCCGGCGCAACCGCCCCCCAGGGAGCCCACGCGGGGGt                                                                                >  3:252128/1‑134 (MQ=255)                                                    cacacaGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCa                                                                              >  4:230614/1‑136 (MQ=255)                                                    cacacaGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGGGGGGTCAGTTGACCGGGCCGGCCCCGGCGCAAGCGCCCCCCGGGTAGACCATGCTCGGGt                                                                                >  8:186005/1‑134 (MQ=255)                                                    cacacaGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCGGGCGTGGGGGGAGTTGGACGGGCCGGCCCCGGCGCAAGCGCCGCCCGGGTTGACCCTGCTCggg                                                                                 >  5:27513/1‑133 (MQ=255)                                                             cTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATcggcggc                                                                      >  5:86954/1‑135 (MQ=255)                                                              tttCGCCGGTACCGTTGCGGGCGTCGACGCTCAGTTAGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTTCACCGGCCGGCCACGGCTCAAGCGCCGCCCAGGTAGACCATTCTCGGGTCATaggcggcg                                                                     >  5:182432/1‑135 (MQ=255)                                                              tttCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATcggcggcgg                                                                    >  7:205130/1‑136 (MQ=255)                                                                tCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTGAGTTGAACGGGCCGGCCCCCGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATcggcggcggc                                                                   >  2:41388/1‑134 (MQ=255)                                                                tCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGt                                                                  >  8:384989/1‑136 (MQ=255)                                                                      gTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGt                                                            <  4:263812/136‑1 (MQ=255)                                                                      gTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGGCAGTTGACCGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGt                                                            <  8:333921/136‑1 (MQ=255)                                                                       tACCGTGGCGGGCGTCGACTATCTGTTCGCAGTTCATGGCCGTGCCTCGTGGCGTGGGGGCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGt                                                            <  2:277659/135‑1 (MQ=255)                                                                               cGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTc                                                    >  2:260898/1‑135 (MQ=255)                                                                                    gTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGt                                                            >  7:327603/1‑122 (MQ=255)                                                                                    gTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGt                                                            <  8:327603/122‑1 (MQ=255)                                                                                               gTTCGCAGTTCATGGCCGTGCGTCCTGGCGTGGGGGCTTTTGAACGGGCCGGCCAGGGGGAAAGCGCCGCCGTGGTAGACGATGCTCGGTTCATCGTCGGCTTGTGCGGGTTGGGCATCCACGGCTGCGCGGAAgg                                   <  8:245946/136‑1 (MQ=255)                                                                                                   gCAGTTCAGGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGttt                                <  1:241960/135‑1 (MQ=255)                                                                                                    cAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCg                              <  8:245439/136‑1 (MQ=255)                                                                                                    cAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCg                              <  7:384989/136‑1 (MQ=255)                                                                                                     aGTTCATGGCCGTGCGTCCTGGGTTGGGGTCAGTTGCACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCg                              <  5:238095/135‑1 (MQ=255)                                                                                                        tCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTa                                                                                              >  1:312438/1‑68 (MQ=255)                                                                                                        tCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTa                                                                                              <  2:312438/68‑1 (MQ=255)                                                                                                            ggCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGt                       <  2:122909/135‑1 (MQ=255)                                                                                                              ccGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTGGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTc                      <  2:12944/134‑1 (MQ=255)                                                                                                              ccGTGCCTCCTGGCGGGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGGCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTc                      <  6:139944/134‑1 (MQ=255)                                                                                                               cGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCt                    >  2:63489/1‑135 (MQ=255)                                                                                                                  gccgccTGGCGTGGGGGCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGGCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTgg                 <  5:208827/135‑1 (MQ=255)                                                                                                                   cctccgGGCGTGGGGTCAGTTGACCGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGGCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGt               <  7:257822/136‑1 (MQ=255)                                                                                                                    ctcctGGCGTGGGGTCAGTTGAACGGGCCGGCCCCGGCGAAAGCGCCGCCCTGGTAGACCATGCGCGGGTCCTCGGCGGCGGGTGCCGGTTGGGCCTCCACCGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGt               >  6:169268/1‑135 (MQ=255)                                                                                                                    ctcctGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGt               <  1:235388/135‑1 (MQ=255)                                                                                                                    ctcctGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGTa              >  7:124525/1‑136 (MQ=255)                                                                                                                    ctcctGGCGGGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGt               <  1:44543/135‑1 (MQ=255)                                                                                                                                 gTCCGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGTACATAGCCCAGGt  <  1:315685/135‑1 (MQ=255)                                                                                                                                 gTCATTTGAACGGCCCGGCCACGTCGAACGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCTGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGTACATAGCCCAGGt  <  1:320804/135‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      AATTGGCGAGCGGGAATGTCTACCCAGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGTACATAGCCCAGGT  >  ACB122/1339010‑1339272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑