Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,377,714	T→C	6.0%	S124G (AGC→GGC)	proS ←	Proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,377,714	0	T	C	6.0%	217.4 / 2.8	67	S124G (AGC→GGC)	proS	Proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (33/30);  new base C (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

TGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGCCGTATTGCTCCCAGCGACCGGATTCCTG  >  ACB122/1377584‑1377843                                                                                                                                   |                                                                                                                                  tGGTCGGCATGGAACGAGTAGGCGTCCTCCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGAc                                                                                                                               <  5:372069/135‑1 (MQ=255)  ggTGGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAg                                                                                                                             >  4:133842/1‑136 (MQ=255)  ggTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACa                                                                                                                              >  6:231967/1‑135 (MQ=255)  ggTCGGCAGGGAACGTGCCGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGGTGAGCGGCAGCTGTTTATAGCTGGACa                                                                                                                              <  1:362334/135‑1 (MQ=255)   gTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACa                                                                                                                              <  7:133432/134‑1 (MQ=255)   gTCGGCATGGAACGAGTAGGCGGCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACa                                                                                                                              <  7:225445/134‑1 (MQ=255)    tCGGCATGGAACTAGTCGGCGTCATTCATGATGAACTCGCGGACACGCATCAGGCCGTAGCGCTGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTCTAGATGGACa                                                                                                                              <  2:225964/133‑1 (MQ=255)      ggCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCg                                                                                                                         <  8:122581/136‑1 (MQ=255)            gAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACg                                                                                                                    <  1:164852/135‑1 (MQ=255)               cGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGcc                                                                                                                <  8:226732/136‑1 (MQ=255)               cGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGcc                                                                                                                <  3:312727/136‑1 (MQ=255)                gaccgggCGTCCTGCAGGATGAAGTCGCGGCCACGCATCCGGCCGAAGCGCGGGCGGAGCGCGGCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCCGGACAGCTCGTTCCGGGCCa                                                                                                               <  4:13836/131‑1 (MQ=255)                     ggCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCt                                                                                                                                   <  7:373963/111‑1 (MQ=255)                     ggCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCt                                                                                                                                   >  8:373963/1‑111 (MQ=255)                     ggCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTc                                                                                                           >  6:2929/1‑135 (MQ=255)                           cTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATc                                                                                                    <  7:50243/136‑1 (MQ=255)                           cTTCATGAGGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATc                                                                                                    <  7:331055/136‑1 (MQ=255)                            ttCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGAt                                                                                                     <  1:111388/134‑1 (MQ=255)                              cATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCAct                                                                                                 >  8:82578/1‑136 (MQ=255)                              cATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCAct                                                                                                 >  8:266237/1‑136 (MQ=255)                              cATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCAct                                                                                                 >  3:338707/1‑136 (MQ=255)                              cATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCAct                                                                                                 >  8:247584/1‑136 (MQ=255)                              cATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCAc                                                                                                  >  6:138990/1‑135 (MQ=255)                                   tgaACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGCGCGGCAGCTGTTTATAGCCGGACAGCTCGTTACGGGCCAGGTCGGGGCTCActctgtc                                                                                            >  3:13836/1‑131 (MQ=255)                                   tgaACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCCGGACAGCTCGTTACGGGCCa                                                                                                               >  3:18732/1‑117 (MQ=255)                                   tgaACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCCGGACAGCTCGTTACGGGCCa                                                                                                               <  4:18732/117‑1 (MQ=255)                                        tCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTggg                                                                                       >  4:106322/1‑136 (MQ=255)                                        tCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTggg                                                                                       >  7:556/1‑136 (MQ=255)                                        tCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTgg                                                                                        >  6:170241/1‑135 (MQ=255)                                          gcgGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTACGTCTGCATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTAACTGGCCAGGTCGGTGATCACTTCTTCGTggg                                                                                       >  4:377781/1‑134 (MQ=255)                                            ggCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAg                                                                                    <  5:164205/135‑1 (MQ=255)                                             gCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAgg                                                                                   >  5:78858/1‑135 (MQ=255)                                             gCCACGCAGCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAgg                                                                                   <  2:283677/135‑1 (MQ=255)                                              ccACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGGTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGc                                                                                 <  3:122525/136‑1 (MQ=255)                                                                 gcgggtggATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCtg                                                              >  3:177629/1‑136 (MQ=255)                                                                 gcgggcggATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGt                                                                                                        >  4:318686/1‑94 (MQ=255)                                                                 gcgggcggATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGt                                                                                                        <  3:318686/94‑1 (MQ=255)                                                                 gcgggcggATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCtg                                                              >  3:349198/1‑136 (MQ=255)                                                                 gcgggcggATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCtg                                                              >  3:369377/1‑136 (MQ=255)                                                                 gcgggcggATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCt                                                               >  2:61919/1‑135 (MQ=255)                                                                  cgggcggATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCtgg                                                             <  3:133842/136‑1 (MQ=255)                                                                   gggcggATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCtgg                                                             <  5:231967/135‑1 (MQ=255)                                                                       ggATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCg                                                        >  3:173839/1‑136 (MQ=255)                                                                       ggATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCg                                                        >  3:173844/1‑136 (MQ=255)                                                                       ggAGCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGc                                                         <  6:368049/135‑1 (MQ=255)                                                                               tCACGGAACTTGGTCTGGAACTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTc                                                 <  1:245119/135‑1 (MQ=255)                                                                               tCACGGAACTTGGGCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTc                                                 <  5:170241/135‑1 (MQ=255)                                                                                    gAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGgcg                                            <  6:127602/135‑1 (MQ=255)                                                                                    gAACTTGGGCTGGATCTGGTACAGGGTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGgcg                                            <  5:146167/135‑1 (MQ=255)                                                                                    gAACTGGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGgcg                                            <  5:66024/135‑1 (MQ=255)                                                                                          ggTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGGGGCGGTCTTTCAGGCgcagca                                       <  4:338707/134‑1 (MQ=255)                                                                                           gTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCagcagc                                     >  5:164460/1‑135 (MQ=255)                                                                                           gTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCagcagc                                     <  8:80672/135‑1 (MQ=255)                                                                                           gTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCt                                    >  3:197332/1‑136 (MQ=255)                                                                                             cTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCagcagc                                     >  2:50907/1‑133 (MQ=255)                                                                                                  tCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGc                              >  1:14625/1‑135 (MQ=255)                                                                                                   cTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGCCg                            >  3:328827/1‑136 (MQ=255)                                                                                                      gTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGcc                             <  7:247584/132‑1 (MQ=255)                                                                                                               gAGCGTCAGCTGTTTATAGCTGGACAGCTCCTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACCCAGAAGACGCGCTGGTCGCGGTCTATCAGGCGCAGCAGCTCAGGGCCGTATTGCTCCCAg                >  4:8374/1‑136 (MQ=255)                                                                                                               gAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGCCGTATTGCTCCCAg                >  4:297645/1‑136 (MQ=255)                                                                                                               gAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGCCGTATTGCTCCCAg                >  8:264301/1‑136 (MQ=255)                                                                                                                   ggCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGgcg                                            >  4:257644/1‑104 (MQ=255)                                                                                                                   ggCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGgcg                                            <  3:257644/104‑1 (MQ=255)                                                                                                                   ggCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGCCGTATTGCTCCCCGCGAc            >  7:388612/1‑136 (MQ=255)                                                                                                                   ggCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGCCGTATTGCTCCCAGCGa             >  1:205280/1‑135 (MQ=255)                                                                                                                      aGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGCCGTATTGCTCCCAGCGACCgg         <  3:89698/136‑1 (MQ=255)                                                                                                                         tGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGCCGTATTGCTCCCAGCGACCGGAtt      >  7:181973/1‑136 (MQ=255)                                                                                                                             tataGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGCCGTATTGCTCCCAGCGACCGGATTCCt   >  1:90081/1‑135 (MQ=255)                                                                                                                              ataGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGCCGTATTGCTCCCAGCGACCGGATTCCTg  <  4:328827/135‑1 (MQ=255)                                                                                                                                   |                                                                                                                                  TGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGTTTATAGCTGGACAGCTCGTTACGGGCCAGGTCGGTGATCACTTCTTCGTGGGTAGGGCCAACGCAGAAGTCGCGCTGGTGGCGGTCTTTCAGGCGCAGCAGCTCAGGGCCGTATTGCTCCCAGCGACCGGATTCCTG  >  ACB122/1377584‑1377843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑