Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,523,480 T→G 22.2% P46P (CCT→CCG lpxC → UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,523,4800TG22.2% 89.5 / 3.1 61P46P (CCT→CCGlpxCUDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase
Reads supporting (aligned to +/- strand):  ref base T (18/22);  new base G (9/6);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATCGA  >  ACB122/1523347‑1523608
                                                                                                                                     |                                                                                                                                
ttAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCtgt                                                                                                                                <  3:190007/136‑1 (MQ=255)
            cccTGAAGAATACCATCCGTGCCACATGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGAACTCGCCCCAGTGGGGGAGAtgc                                                                                                                     >  6:375511/1‑133 (MQ=255)
                  agaaTACCATCCGTGCCCCAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTgcg                                                                                                                <  2:23464/134‑1 (MQ=255)
                         cATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGCCACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCCGAGGTCGAGCTCCCTGCACGTGCGGc                                                                                                        >  6:62060/1‑135 (MQ=255)
                          aTCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCTACTTCCCCCCCGCCGACCTCGACCCAGTGGTCCAGCTCCCAGCGCGGGCGcccc                                                                                                      >  8:89274/1‑135 (MQ=255)
                          aTCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGCCCTCGCCCCTGTGGTCGAGATCCCTGCGCGTGCGGCCa                                                                                                      >  3:336820/1‑136 (MQ=255)
                          aTCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGCCCCTGTGGTCGAGATCCCTGCGCGTGCGGCCa                                                                                                      >  4:335031/1‑136 (MQ=255)
                          aTCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCACGACCCGCTGGTCGAGATCCCTGCGCGGGCGGCCa                                                                                                      >  7:188195/1‑136 (MQ=255)
                              gTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTCCCTGAACCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCCCGCCGACCTCGCCCCGGTGGTCGCTATTCCGGCGCGTTCGGccccac                                                                                                   >  2:132563/1‑131 (MQ=255)
                              gTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGGCGCCCTCGCCCCGGGGGGGGGGAGCCCTGGGCGTGCGGcccccc                                                                                                   >  1:367212/1‑131 (MQ=255)
                              gTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGCCCTCCCCCCTGGGGTCGAGCTCCCGGGGCGTGCGGCCCACg                                                                                                   >  5:176364/1‑135 (MQ=255)
                               tGCCACAGGTGTCGGGCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGt                                                                                                  <  6:237424/135‑1 (MQ=255)
                                        tgtCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGCCCTCGACCCTGTGGTCGAGCTCCCTGCGCGTGCGGCCAACGTCGGCGAGCCa                                                                                        >  4:285120/1‑136 (MQ=255)
                                        tgtCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGCCCACGCCCCGGTGGGCGAGCACCCTGCGCGTGCGGCCCACCTCGGCgggcc                                                                                         >  1:288350/1‑131 (MQ=255)
                                             gTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGCCCACGCCCCTGTGGTCGCGCTCCCTGCGCGTGCGGCCCACGTCGGCGAGGCACCCAt                                                                                   >  8:154719/1‑136 (MQ=255)
                                               cTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGt                                                                                 <  7:166016/136‑1 (MQ=255)
                                                    cTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTcgacg                                                                            <  8:121976/136‑1 (MQ=255)
                                                    cTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTcgacg                                                                            <  8:116573/136‑1 (MQ=255)
                                                    cTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTcgacg                                                                            <  8:244314/136‑1 (MQ=255)
                                                     tCCGGGGAGAAGGTTTACCTGCCCCTCACCCCTGCACCTGTTGCCCCCGGCATCGTCGTCCGCCGCGCCGACCTCGACCCGGTGGGCGAGATCCCTGGGCGCGCGGCCAACGTCGGCGAGACAACCATGTcgacg                                                                            <  6:363770/135‑1 (MQ=255)
                                                       cGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCACCCCCCAGTGGGGGAGCTACCTGCGCGTGCGGCCCAACTCGGCGCGCCAACCCAGTAgacgac                                                                          >  6:239810/1‑135 (MQ=255)
                                                          ggAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGCCCCTGTGGTCGAGCTCCCGGCGCGTGCGGCCAACGTCGGCGAGACAACCCTGTCGACGACGCt                                                                       >  2:269428/1‑135 (MQ=255)
                                                             gaAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCCCGCCGACCAACACCCTGTGGTCGAGATCCCTGCGCGTGGGGCCAACCTCGGCGCGACAACCAAGTCGACGACGCAggg                                                                    >  5:315493/1‑134 (MQ=255)
                                                                 gTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAAc                                                                <  6:207027/135‑1 (MQ=255)
                                                                    tACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGCCCGCGCCCCGGGGGTGGAGGTCCCTGCGCGTGGGGCCCACCTCGGCGCGACCACCAGTTCGACGCCGCGGGGCCACggg                                                             >  2:359698/1‑134 (MQ=255)
                                                                    tACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCAGCCGCCCCGCCCACGCCCCTGTTGTCGAGATACCGGCGCCTGCGGCCAACGTAGGCGAGCCAACCAAGTCGACGCCGCTGGTaaaagct                                                             >  6:144702/1‑131 (MQ=255)
                                                                        tGACCCCCACGCCGCCACGTGTGGACACCGGCTGCGTCTCCCGCCGCGCCTACCTCGACCCGGGGTGCGAGATCCCTGCGCGTGGGGCCAACGGCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACg                                                         <  5:62060/135‑1 (MQ=255)
                                                                           cccTCACGCCTGCACCTGTTGACACCGGCAGCGGCTGCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCaa                                                     <  3:261546/136‑1 (MQ=255)
                                                                             cTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGCCCCGGTGGTGGCGATCCCTGCGCGGGCGGCCCACCTCGGCGCGCCCACCCAGTCGACGACGCGGGTCCACGGGGGCGGCAAg                                                    >  5:295861/1‑135 (MQ=255)
                                                                             cTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCAGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAgg                                                   >  3:57213/1‑136 (MQ=255)
                                                                                aaGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCg                                                <  7:340403/136‑1 (MQ=255)
                                                                                aaGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCg                                                <  3:204079/136‑1 (MQ=255)
                                                                                aaGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGGGCGGCCAACGTCGGCGAGACAACCATGGCGACGACGCTGGTCAACGGTGACGTCAAGGTCg                                                <  7:96219/136‑1 (MQ=255)
                                                                                 aGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCg                                                <  5:290661/135‑1 (MQ=255)
                                                                                          cTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGCCCCGGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTc                                        >  4:239844/1‑134 (MQ=255)
                                                                                          cTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATACCTGCGCGGGCGGCCCACGTCGGCGCGACAACCATGTCGACGCCGCTGGTCAACGGTGACGTGAAGGTGGATACGGt                                         >  1:1333/1‑133 (MQ=255)
                                                                                             ttGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAg                                     <  4:80331/134‑1 (MQ=255)
                                                                                             tggcccccGGCAGCGGCTTCCCCCGCCCCGCCCACCCCCGGGGGGGCGTGATCCCGGCGCGGGCGGCCAACGGCGGCGAGACAACCATGCCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGc                                    <  6:45277/129‑1 (MQ=255)
                                                                                                acacCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCt                                >  7:374880/1‑136 (MQ=255)
                                                                                                      gCAGCGTGTCCCCCCGCGCGCACCGCGACCCGGTGGCCTAGATCCCTGCGCGTGCGGCAACCGCCGGCCCCCAAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCtctc                          <  8:155318/136‑1 (MQ=255)
                                                                                                       cagcGTCTGCCGCCGCGCCGACCTCGACCCTGTGGGCGAGATCCCTGCGCGTGCGGCCAACGGCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCtctc                          <  5:42858/132‑1 (MQ=255)
                                                                                                       cATCGTCTTCCGCCGCGCCGACCTCGCCCCAGTGGTCGGGATACCTGCGCGTGCGGCCAACGTCGGCGAGACAACCAAGTAGACGCCCCTGGGCAAAGGTGTCGTCAAGGTCGAAACGGTCGCGCCACTGCtctc                          >  2:95518/1‑135 (MQ=255)
                                                                                                             cTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCAt                    <  6:315493/135‑1 (MQ=255)
                                                                                                                   ccgcgccgACCTCGCCCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACCTAGGCCAGCCAACCATGTCGACGCAGCTGGTCAACGGTGACGGCAAGGTAGATACGGTCGAGCACCTGCTCTCCCCCATGGCggg              >  6:199219/1‑135 (MQ=255)
                                                                                                                   ccgcgccgACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCg                                                >  7:119662/1‑101 (MQ=255)
                                                                                                                   ccgcgccgACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCg                                                <  8:119662/101‑1 (MQ=255)
                                                                                                                    cgcgccgACCTCGACCCGGTGGTCGAGATCCCTGCGCGGGCGGCCAACGTCGGCGAGACAACCATGGCGCCGACGCGGGGCCACGGGGACGTCAATGTCGCTACGGTCGCGCACCTGCTCTCCGCCAAGGCggggc            >  8:3761/1‑134 (MQ=255)
                                                                                                                     gcgccgACCTCGACCCTGTGGTCGAGATCCCGGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCt           <  8:48947/136‑1 (MQ=255)
                                                                                                                       gccgACCTCGACCCTGGGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTgg         <  3:30527/136‑1 (MQ=255)
                                                                                                                         cgACCTCGCCCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCGGGTCAACGGTGACGTCAAGGTCGATACGGTCGCGCACCGGCTCTCCGCCATGGCGGGGCTGGGc       >  7:41642/1‑136 (MQ=255)
                                                                                                                         cgACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGCCGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGGCTGGGc       >  3:225524/1‑136 (MQ=255)
                                                                                                                         cgACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCt                                <  1:323047/111‑1 (MQ=255)
                                                                                                                         cgACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCt                                >  2:323047/1‑111 (MQ=255)
                                                                                                                         cgACCTCGACCCTGTGGTCGAGATCCCGGCGCGTGCGGCCAACGACGGCGAGACAACCATGTCGACGACGCTGGGCGACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCAGGGCGGGGCTggg        >  5:167659/1‑135 (MQ=255)
                                                                                                                         cgACCTCGACCCGGTGGTCGCGCTCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCAAGTCCACGACGCAGGTCAACGGTTCCGTCAACGTCGATACCGTCGAGCACCTGCTCTCCGCCATGGCGGGTCttg         >  2:284384/1‑132 (MQ=255)
                                                                                                                            ccTCGACCCTGTGGTGGAGATCCCTGGGCGTGCGGCCAACGTCGGCGAGACAACCTTGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATc    <  7:181200/136‑1 (MQ=255)
                                                                                                                             cTCGCCCCGGGGGGCGAGAGCCCTGCGGGTGCGGCCACCGTCGGCTAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATc    <  5:239810/135‑1 (MQ=255)
                                                                                                                             cTCGAGCCGGTGGTCGAGATCCGTGTGCGTGCGGAGAAGGTGGGCGAGACAACCATGGCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATTCGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATc    <  1:284384/135‑1 (MQ=255)
                                                                                                                             cTCGACCCTGTGGTCGAGATCCCTGCGCGTGGGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATc    <  1:16230/135‑1 (MQ=255)
                                                                                                                             cTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATc    <  6:64755/135‑1 (MQ=255)
                                                                                                                              tCGCCCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATCGa  >  3:115965/1‑136 (MQ=255)
                                                                                                                              tCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCCATACGGTCGAGCACCAGCTCTCCGCCATGGCGGGTATGGGCCTCg   >  4:64256/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                
TTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATCGA  >  ACB122/1523347‑1523608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: