Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,718,463 T→G 29.6% Q65P (CAG→CCG)  CJ019_01614 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,718,4630TG29.6% 35.9 / 5.0 53Q65P (CAG→CCG) CJ019_01614hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (13/23);  new base G (14/3);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 2.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTGATGAACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGG  >  ACB122/1718332‑1718596
                                                                                                                                   |                                                                                                                                     
tATTGATGAACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGgcg                                                                                                                                   <  8:126520/136‑1 (MQ=255)
tATTGATGAACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGgc                                                                                                                                    >  1:88809/1‑135 (MQ=255)
  ttGATGAACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGgcgc                                                                                                                                  <  4:67942/135‑1 (MQ=255)
  ttGATGAACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGGCATAGGGGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGgcgc                                                                                                                                  <  7:38618/135‑1 (MQ=255)
   tgatgaACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGCCCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGgcgc                                                                                                                                  <  5:351880/134‑1 (MQ=255)
   tgatgaACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGgcgc                                                                                                                                  <  2:36039/134‑1 (MQ=255)
      tcaccAGCAACTTGCCATTCAGCTCAATGCGCGCACTGACCGCATCGGGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAg                                                                                                                             <  4:313299/132‑1 (MQ=255)
        aaCAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCCGGCTTCCCCTGCGCGGGGTCTTAGGTGAGATGGAACGGCAGGGGCACGTTGCCTTTGACCGGGCGGGCCTGGCGCGCCAg                                                                                                                             <  7:380570/134‑1 (MQ=255)
        aaCAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCGGGGCGGGCCCggg                                                                                                                           >  3:176067/1‑136 (MQ=255)
           aGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTc                                                                                                                         >  2:22712/1‑135 (MQ=255)
             cAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGGCACg                                                                                                                      >  7:147271/1‑136 (MQ=255)
             cAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGCGGCAc                                                                                                                       >  1:318268/1‑135 (MQ=255)
                cTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCAccg                                                                                                                     >  8:134978/1‑132 (MQ=255)
                 ttGCCATTCAGCTCAATGCGCGCAGTTCCCGCATAGCGGTGGCCAGGCTTGACCGGCGCGGGGTCATAGGTGAGATGGAACGCCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCa                                                                                                                   <  2:267360/135‑1 (MQ=255)
                          aGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTcc                                                                                                          <  4:299944/135‑1 (MQ=255)
                          aGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCAGAGGTGAGATGGAACGGCAGCGGCCCGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTCCCGGCAGGGGCGTc                                                                                                           >  3:291567/1‑134 (MQ=255)
                                 tGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCGGGGCGCGCCAGGGGCCCGGCAGGGGCGGCCCGCAGCgc                                                                                                  >  3:283083/1‑135 (MQ=255)
                                  gcgcgcACTACCCGCATAGCGGGGGCCAGGCTTGCCGGGCGGGGGGGCATAGGTGAGCGGGACCGGCCGCGGCCCGTGGCCTTTGCCCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGac                                                                                                 <  3:354001/136‑1 (MQ=255)
                                        aCTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATc                                                                                            <  1:47899/135‑1 (MQ=255)
                                         cTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGTAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCTGGCGCCCCCGGCTCACGGGCCGGGCGTGCAGCACCGCCACAt                                                                                             >  6:168144/1‑133 (MQ=255)
                                             ccGCATAGCGGTGGCCAGGCTTGACCGTCGCGGGGGCATAGGTGAGAGGGAACGGCAGCGGCACGGTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAg                                                                                      <  8:276440/136‑1 (MQ=255)
                                              cGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGCGCGCCCGGGGCCCGGCCGGGGGGTCCATGCGCGCCACAACCAGCacg                                                                                     >  3:198414/1‑134 (MQ=255)
                                                   aGCGGTGGCCAGGCTTGACCGGCGCGGGGTCATAGGGGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCAc                                                                                <  7:166329/136‑1 (MQ=255)
                                                     cGGTGGCCAGGCTTGACCTGCGCGGGGTCTTAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGGGCGGCACGGGGACGGCCGGGGCGGCCCTCCGCGCCCCAACCTGCAGGTGCACGc                                                                              >  3:29247/1‑136 (MQ=255)
                                                     cGGTGGCCAGGCTTGACCTGCGCGGGGTCTTAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCGCGGCAGGGGGGGCCCGCCGCGCCACCTCCGGGAGTTCCCCg                                                                               >  6:10900/1‑135 (MQ=255)
                                                       gTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGGCCCGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCt                                                                             >  8:192483/1‑135 (MQ=255)
                                                           ccAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGCGCGCCCGGGGCCCGGCCGGGGCGGCGCGCCGCGCCCCCTCCGGCAGGTGCACGCGCAggg                                                                        >  7:10010/1‑136 (MQ=255)
                                                           ccAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCCGGGGCGTCCATCAGCGCCCCCTCCGGCAGTTCCACGCTCAgg                                                                         >  2:214374/1‑135 (MQ=255)
                                                             aGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGTGGCGGGCGGGGCGTCCCTGAGCGACCGCTCCGGCCGGTCCCCGCGCAGGGt                                                                       >  2:22363/1‑135 (MQ=255)
                                                                 ttGACCTGCGCGGGGTCATAGGGGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcg                                                                   <  2:37897/135‑1 (MQ=255)
                                                                  tGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggc                                                                 >  8:329839/1‑136 (MQ=255)
                                                                  tGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTGCCGGCCGGGGCGTCCATAAGCGCCCCCACCAGCCGGTCCCCGCTCAGGGTggcgg                                                                  >  2:90937/1‑135 (MQ=255)
                                                                   gACCTGCGCGGGGTCATAGGGGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggc                                                                 <  8:238463/135‑1 (MQ=255)
                                                                     ccTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                              <  3:320569/136‑1 (MQ=255)
                                                                     ccTGCGCGGGGGCATAGGGGAGAGGGAACGGCCCCGGCACGTTGCCTTGGACCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                              <  3:147589/136‑1 (MQ=255)
                                                                       agcgcGGGGGCATCGGGGAGATGGACCGGCAGCGGCACGTGGCCTTTGACCGGCCCGGCCGGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcaggcg                                                            <  7:320097/135‑1 (MQ=255)
                                                                            ggggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGCGCGGCCGGGGCCCGGGCGGGGCGGGCCTCCGCGCCACCTCCTGCAGGTCCCCGCGCAGGGTggcggccggggggcgg                                                       >  7:227728/1‑132 (MQ=255)
                                                                            ggggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGGCACGGGCGGGGCGGCGCTCAGCGCCACATCCCGCCGTTGCACGCTCCGGGgggcggcgggcggccg                                                        >  2:187165/1‑133 (MQ=255)
                                                                            ggggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGGGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGcggcaggcggcagg                                                       >  4:95219/1‑136 (MQ=255)
                                                                            ggggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGcggcaggcggcagg                                                       >  8:181382/1‑136 (MQ=255)
                                                                            ggggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGGGCATCAGCGCCACATCCTGGACTTCCACCCGCACGGTGgcggcaggcggcag                                                        >  2:299937/1‑135 (MQ=255)
                                                                             gggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGCGTCACGGCAGGGGCGTCCAACAGCCCCCCATCCTGCACATCCACGCACAGCGTGGcggcacgcggcagg                                                       >  5:83414/1‑135 (MQ=255)
                                                                                   gaGGTGAGAGGGAACGGCAGCGGCACGTTGCCTGTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAAt                                                  <  2:88809/133‑1 (MQ=255)
                                                                                    aGGTGAGATGGACCGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATgc                                                <  4:29247/135‑1 (MQ=255)
                                                                                           aTGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGTCACGGCAGGGGCGTCCATCAGCg                                                                                                   >  4:125310/1‑77 (MQ=255)
                                                                                           aTGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCg                                                                                                   <  3:125310/77‑1 (MQ=255)
                                                                                           aTGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAg                                         <  5:383829/135‑1 (MQ=255)
                                                                                             ggAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGGCCCGGCCGGGGCGGCCATCAGCGCCACCACCGGCAGGTCCACGCGCCCGGGGGCGGGCGGCGGCCGGGGCATGCGCTGCAg                                         >  1:309880/1‑133 (MQ=255)
                                                                                                    cAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                               <  4:283083/136‑1 (MQ=255)
                                                                                                                  cTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCt                 <  7:181382/136‑1 (MQ=255)
                                                                                                                              cggccGGGCGCGCCCGGGGCACGGCAGGGGCGGCCCTCAGCGCCCCATCCTGCAGTTCCACGCGCAGGGTGGCGGCCGGCGGCCGGGCCATGCGCGGCCGGGAGAAGACTTCACCCGCCAGGCGGGGCGGGGtgg      >  2:287711/1‑135 (MQ=255)
                                                                                                                                 ccTGGCGCGCCTGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTtgggtgg  <  3:218534/136‑1 (MQ=255)
                                                                                                                                  cTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTtgggtgg  <  2:159892/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
TATTGATGAACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGG  >  ACB122/1718332‑1718596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑