Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,791,678 T→G 26.5% L182R (CTG→CGG)  lpxB → Lipid‑A‑disaccharide synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,791,6780TG26.5% 75.4 / 9.5 68L182R (CTG→CGG) lpxBLipid‑A‑disaccharide synthase
Reads supporting (aligned to +/- strand):  ref base T (41/4);  new base G (4/16);  total (48/20)
Fisher's exact test for biased strand distribution p-value = 2.60e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACCTGATGCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTG  >  ACB122/1791544‑1791811
                                                                                                                                      |                                                                                                                                      
cGACCTGATGCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGCTGTGCCAGGGCGATTTGTCGGCCACCCGCTGGCAGACACCAAACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGCCGCGctg                                                                                                                                       <  8:138580/136‑1 (MQ=255)
              cgcgGCGGCCGTGCGCGGCGCGGTTCTACGACGCGCCGGGGGTGCCCGGGCGTTTGGGCGGCCACCCGCGGGCACCCACCATCCCGCTTGAAGCCGACCGCCCGCCCGCGCGGGCCGCGCGGGGCCGGGGCGAgg                                                                                                                          <  5:106931/135‑1 (MQ=255)
                ctgctgCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGc                                                                                                                       >  7:3724/1‑136 (MQ=255)
                  gcggcCGTGCGCGGGGCGGTGCTCCGAAGCGCTGGGTGGGCCAGGGCGTTTTGGCGGCCACCCGCTGGCAGACACCATACCGCTTGACGCCGCCCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGCGGGGCCg                                                                                                                     <  8:287596/133‑1 (MQ=255)
                      ccGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCggtgg                                                                                                                 >  4:322720/1‑136 (MQ=255)
                            gAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACTCCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCg                                                                                                           >  2:233019/1‑135 (MQ=255)
                               gacaggTCCTACGAAGAGCAGGGGGGGCCAGTGCGTTTTGGCGGCCACCCGCTGGCACACACCATCCCGCTTGACGCCGACCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTg                                                                                                        <  6:391650/131‑1 (MQ=255)
                                   ggTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGc                                                                                                    >  2:279065/1‑135 (MQ=255)
                                   ggTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCTGGGCGCGGGGCCCGTGGTGGCGGTGGTGcc                                                                                                   >  3:35306/1‑136 (MQ=255)
                                   ggTTCGACGAAGAGCAGGGTGTGCCAGTGCGGTTTGTCGGCCACCCGCTGGCAGCCACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCCGGCCGCGCAGGGCCGGGGCGAGGGGCCGGTGGTGGCGGTGCTGcc                                                                                                   >  3:40451/1‑136 (MQ=255)
                                       cTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGGGGTGGCGGTGATGCCGGGc                                                                                               >  8:207657/1‑136 (MQ=255)
                                              gcgCAGGGTGTGCCAGTGCGTGTGGGCGCCCACCCGCTGGCAGACACCATCCCGCGTGACGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCggg                                                                                        <  4:260544/134‑1 (MQ=255)
                                              gagCAGGGGGGGCCCGGGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTGCACGCCGACCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCggg                                                                                        <  8:13595/136‑1 (MQ=255)
                                                 cAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCAGGGCCTGGGCGAGGGGCCGGAGGTGGCGGTGATGCCGGGCAGCCGGGGt                                                                                      >  5:151816/1‑135 (MQ=255)
                                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGGCGGGGCGCGGGGCCGGTGGTGGGGGTGATGGCGGGCCGCCGgggggg                                                                                    >  7:145068/1‑136 (MQ=255)
                                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGGTGATGCCGGGCCGCCgcgggc                                                                                     >  5:391650/1‑133 (MQ=255)
                                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTgg                                                                                    >  3:313841/1‑136 (MQ=255)
                                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTgg                                                                                    >  7:334036/1‑136 (MQ=255)
                                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTgg                                                                                    >  4:166992/1‑136 (MQ=255)
                                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTgg                                                                                    >  3:260544/1‑136 (MQ=255)
                                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  1:26264/1‑135 (MQ=255)
                                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  1:29432/1‑135 (MQ=255)
                                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  6:268472/1‑135 (MQ=255)
                                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGGCGCGCGGGGGCGGGGGGGGGGGGCGGGGGGGGGGGTGGTGGCGGGCCGCCgggggg                                                                                     >  5:337066/1‑133 (MQ=255)
                                                    ggTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCCCCTGCCGCGCGGGGCCGGGGCGCGGGGCCGGGGGTGGGCGTTCTGCCGGGCCGCCgggggtgcg                                                                                  >  8:144684/1‑131 (MQ=255)
                                                      ggtgCCTGTGCGTTTTGGCGGCCACCCGCGGGCAGACACCATACCGCTTGACGCCGCCCGCCGGGCGGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGaa                                                                                <  4:229527/135‑1 (MQ=255)
                                                       gtgCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGCAg                                                                               >  3:321300/1‑136 (MQ=255)
                                                            aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGGTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                           >  6:157389/1‑135 (MQ=255)
                                                            aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCAGACCGCCCGGCCGCGCGTGCCGCGCTGGCCCTGGGCGAGGGGGCGGGGGTGGGGGTGATGGCGGGCAGCCCGGGTGGCGCAGTAggg                                                                          >  3:234714/1‑136 (MQ=255)
                                                                 gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCCGCCGGGGTGGCGAAGTAGGGCGCt                                                                      >  6:90578/1‑135 (MQ=255)
                                                                 gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGGGAAGTAGGGCGCt                                                                      >  1:14458/1‑135 (MQ=255)
                                                                 gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCt                                                                      >  6:153760/1‑135 (MQ=255)
                                                                 gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGCCGCGCTGGGCCGGGGCGCGGGGCCGGTGGTGGCGTTGATGGCGGGCAGCCGGGGTGGCGAAGTAGGGCGCtt                                                                     >  3:273110/1‑136 (MQ=255)
                                                                   tttGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTgg                                                                   >  3:373256/1‑136 (MQ=255)
                                                                     tGTCGCCCCCCCGCTGGCAGACACCATACCGGTTGACGCCGACCGCCCGGCCGGGCGTGCCGCGGGGGGCCTGGGCTAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                 <  8:147389/136‑1 (MQ=255)
                                                                      ggcGGCCACCCGCTGGCAGACACCATACCGCTTGACGCCGCCCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgcg                                                                <  7:207657/134‑1 (MQ=255)
                                                                       tCGGCCACCCGCTGGCAGACACCATACCGCTTGACGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                 <  6:205289/134‑1 (MQ=255)
                                                                       gcGGCCACCCGCTGGCAGACACCATCCGGCTGGACGCCGCCCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                 <  2:292701/133‑1 (MQ=255)
                                                                                cGCTGGCAGACACCATACCGCTTGACGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCt                                                      <  7:117623/136‑1 (MQ=255)
                                                                                                  cGCTTGAAGCCGACCGCCCGGCCGCGCGTGGCGCGCGGGGCCGGGGGGCGGGGGCGGTGGTGGGGTTTATGCCGGGCAGCCGGGGTGGCGAAGGTGGGCGCTTGGGCGGGCTGTTACACGACCGCGCcgcgcggc                                     >  2:139788/1‑133 (MQ=255)
                                                                                                  cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCATCCGGGGTGGCGAAGTAGGGCGCTTTGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCt                                    >  8:223666/1‑136 (MQ=255)
                                                                                                  cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGcc                                     >  6:164880/1‑135 (MQ=255)
                                                                                                  cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGCTTGTGGCGTTGATGCCGGGCCGCCGGGGTGGCGAAGTAGGGCGCTTTGGCGCGCTGTTCCTCGACGCCGCCGAGCGcc                                     >  6:70780/1‑135 (MQ=255)
                                                                                                  cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCAGGGCGACGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCGGGTGCTCGCCGCCGCCGAGCGCCt                                    >  7:129818/1‑136 (MQ=255)
                                                                                                   gCTTGACGCCGCCCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCt                                    <  6:337066/135‑1 (MQ=255)
                                                                                                     ttGACGCCCCCCGCCCGGCCGCGCGCGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTCCCTCGACGCCGCCGAGCGCCTCa                                  <  5:58004/135‑1 (MQ=255)
                                                                                                       gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAg                                 >  1:127804/1‑134 (MQ=255)
                                                                                                       gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGcc                               >  8:112077/1‑136 (MQ=255)
                                                                                                       gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                >  6:376475/1‑135 (MQ=255)
                                                                                                       gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                >  4:346460/1‑135 (MQ=255)
                                                                                                       gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTGGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGGTCCTCGCCGCCGCCGCGCGCCTCAGc                                >  2:254462/1‑135 (MQ=255)
                                                                                                       gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCAGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                >  8:263973/1‑135 (MQ=255)
                                                                                                        ccgCCGACGGCCCGGCCGCGGGGGCCGCGCGGGGCGGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                              <  3:372188/134‑1 (MQ=255)
                                                                                                          gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTTGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCAGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                             >  5:104499/1‑135 (MQ=255)
                                                                                                          gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                             >  7:54480/1‑135 (MQ=255)
                                                                                                          gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                             >  8:233264/1‑135 (MQ=255)
                                                                                                          gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                             >  3:296199/1‑135 (MQ=255)
                                                                                                          gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                             >  4:186246/1‑135 (MQ=255)
                                                                                                          gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                             >  8:100671/1‑135 (MQ=255)
                                                                                                            cGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                             >  1:5224/1‑133 (MQ=255)
                                                                                                             gACCGCCCGGCCGCGCGGGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAg                         <  4:127423/136‑1 (MQ=255)
                                                                                                                 gCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGCTGCCGGGCCGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGCGCGCCTCAGCCAACAAGTGc                      >  5:190645/1‑135 (MQ=255)
                                                                                                                 gCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGcc                     >  8:206217/1‑136 (MQ=255)
                                                                                                                   cgggCCGCGCGGGCGGGGGGGGGCCGGGGCTCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCgg                   <  7:338548/134‑1 (MQ=255)
                                                                                                                         gggggggCCGCGCGGGGCCGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc             <  3:17672/130‑1 (MQ=255)
                                                                                                                         gcgcgTGGCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc             <  4:234714/136‑1 (MQ=255)
                                                                                                                          ggcgTGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc             <  1:323049/134‑1 (MQ=255)
                                                                                                                               gCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGCGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCg       >  7:305716/1‑136 (MQ=255)
                                                                                                                               gCCGCGCGGGGCCGGGGCGAGGGGCCGGGGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTc        <  5:89224/135‑1 (MQ=255)
                                                                                                                                     ctgggcctgggcGAGGGGCCGGTGGTGTCGTTGATGCCGGGCAGCCTGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTg  <  6:151816/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CGACCTGATGCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTG  >  ACB122/1791544‑1791811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: