Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,791,684 T→G 24.4% L184R (CTG→CGG)  lpxB → Lipid‑A‑disaccharide synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,791,6840TG24.4% 94.1 / 3.0 68L184R (CTG→CGG) lpxBLipid‑A‑disaccharide synthase
Reads supporting (aligned to +/- strand):  ref base T (41/7);  new base G (6/13);  total (48/20)
Fisher's exact test for biased strand distribution p-value = 3.70e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTGCCCTGCG  >  ACB122/1791558‑1791818
                                                                                                                              |                                                                                                                                       
cgcgGCGGCCGTGCGCGGCGCGGTTCTACGACGCGCCGGGGGTGCCCGGGCGTTTGGGCGGCCACCCGCGGGCACCCACCATCCCGCTTGAAGCCGACCGCCCGCCCGCGCGGGCCGCGCGGGGCCGGGGCGAgg                                                                                                                                 <  5:106931/135‑1 (MQ=255)
  ctgctgCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGc                                                                                                                              >  7:3724/1‑136 (MQ=255)
    gcggcCGTGCGCGGGGCGGTGCTCCGAAGCGCTGGGTGGGCCAGGGCGTTTTGGCGGCCACCCGCTGGCAGACACCATACCGCTTGACGCCGCCCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGCGGGGCCg                                                                                                                            <  8:287596/133‑1 (MQ=255)
        ccGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCggtgg                                                                                                                        >  4:322720/1‑136 (MQ=255)
              gAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACTCCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCg                                                                                                                  >  2:233019/1‑135 (MQ=255)
                 gacaggTCCTACGAAGAGCAGGGGGGGCCAGTGCGTTTTGGCGGCCACCCGCTGGCACACACCATCCCGCTTGACGCCGACCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTg                                                                                                               <  6:391650/131‑1 (MQ=255)
                     ggTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGc                                                                                                           >  2:279065/1‑135 (MQ=255)
                     ggTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCTGGGCGCGGGGCCCGTGGTGGCGGTGGTGcc                                                                                                          >  3:35306/1‑136 (MQ=255)
                     ggTTCGACGAAGAGCAGGGTGTGCCAGTGCGGTTTGTCGGCCACCCGCTGGCAGCCACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCCGGCCGCGCAGGGCCGGGGCGAGGGGCCGGTGGTGGCGGTGCTGcc                                                                                                          >  3:40451/1‑136 (MQ=255)
                         cTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGGGGTGGCGGTGATGCCGGGc                                                                                                      >  8:207657/1‑136 (MQ=255)
                                gcgCAGGGTGTGCCAGTGCGTGTGGGCGCCCACCCGCTGGCAGACACCATCCCGCGTGACGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCggg                                                                                               <  4:260544/134‑1 (MQ=255)
                                gagCAGGGGGGGCCCGGGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTGCACGCCGACCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCggg                                                                                               <  8:13595/136‑1 (MQ=255)
                                   cAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCAGGGCCTGGGCGAGGGGCCGGAGGTGGCGGTGATGCCGGGCAGCCGGGGt                                                                                             >  5:151816/1‑135 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGGCGGGGCGCGGGGCCGGTGGTGGGGGTGATGGCGGGCCGCCGgggggg                                                                                           >  7:145068/1‑136 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGGTGATGCCGGGCCGCCgcgggc                                                                                            >  5:391650/1‑133 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTgg                                                                                           >  3:260544/1‑136 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTgg                                                                                           >  3:313841/1‑136 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTgg                                                                                           >  7:334036/1‑136 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTgg                                                                                           >  4:166992/1‑136 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                            >  1:26264/1‑135 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                            >  6:268472/1‑135 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                            >  1:29432/1‑135 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGGCGCGCGGGGGCGGGGGGGGGGGGCGGGGGGGGGGGTGGTGGCGGGCCGCCgggggg                                                                                            >  5:337066/1‑133 (MQ=255)
                                      ggTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCCCCTGCCGCGCGGGGCCGGGGCGCGGGGCCGGGGGTGGGCGTTCTGCCGGGCCGCCgggggtgcg                                                                                         >  8:144684/1‑131 (MQ=255)
                                        ggtgCCTGTGCGTTTTGGCGGCCACCCGCGGGCAGACACCATACCGCTTGACGCCGCCCGCCGGGCGGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGaa                                                                                       <  4:229527/135‑1 (MQ=255)
                                         gtgCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGCAg                                                                                      >  3:321300/1‑136 (MQ=255)
                                              aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGGTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                                  >  6:157389/1‑135 (MQ=255)
                                              aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCAGACCGCCCGGCCGCGCGTGCCGCGCTGGCCCTGGGCGAGGGGGCGGGGGTGGGGGTGATGGCGGGCAGCCCGGGTGGCGCAGTAggg                                                                                 >  3:234714/1‑136 (MQ=255)
                                                   gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCCGCCGGGGTGGCGAAGTAGGGCGCt                                                                             >  6:90578/1‑135 (MQ=255)
                                                   gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGGGAAGTAGGGCGCt                                                                             >  1:14458/1‑135 (MQ=255)
                                                   gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCt                                                                             >  6:153760/1‑135 (MQ=255)
                                                   gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGCCGCGCTGGGCCGGGGCGCGGGGCCGGTGGTGGCGTTGATGGCGGGCAGCCGGGGTGGCGAAGTAGGGCGCtt                                                                            >  3:273110/1‑136 (MQ=255)
                                                     tttGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTgg                                                                          >  3:373256/1‑136 (MQ=255)
                                                       tGTCGCCCCCCCGCTGGCAGACACCATACCGGTTGACGCCGACCGCCCGGCCGGGCGTGCCGCGGGGGGCCTGGGCTAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                        <  8:147389/136‑1 (MQ=255)
                                                        ggcGGCCACCCGCTGGCAGACACCATACCGCTTGACGCCGCCCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgcg                                                                       <  7:207657/134‑1 (MQ=255)
                                                         tCGGCCACCCGCTGGCAGACACCATACCGCTTGACGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                        <  6:205289/134‑1 (MQ=255)
                                                         gcGGCCACCCGCTGGCAGACACCATCCGGCTGGACGCCGCCCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                        <  2:292701/133‑1 (MQ=255)
                                                                  cGCTGGCAGACACCATACCGCTTGACGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCt                                                             <  7:117623/136‑1 (MQ=255)
                                                                                    cGCTTGAAGCCGACCGCCCGGCCGCGCGTGGCGCGCGGGGCCGGGGGGCGGGGGCGGTGGTGGGGTTTATGCCGGGCAGCCGGGGTGGCGAAGGTGGGCGCTTGGGCGGGCTGTTACACGACCGCGCcgcgcggc                                            >  2:139788/1‑133 (MQ=255)
                                                                                    cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCATCCGGGGTGGCGAAGTAGGGCGCTTTGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCt                                           >  8:223666/1‑136 (MQ=255)
                                                                                    cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGcc                                            >  6:164880/1‑135 (MQ=255)
                                                                                    cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGCTTGTGGCGTTGATGCCGGGCCGCCGGGGTGGCGAAGTAGGGCGCTTTGGCGCGCTGTTCCTCGACGCCGCCGAGCGcc                                            >  6:70780/1‑135 (MQ=255)
                                                                                    cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCAGGGCGACGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCGGGTGCTCGCCGCCGCCGAGCGCCt                                           >  7:129818/1‑136 (MQ=255)
                                                                                     gCTTGACGCCGCCCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCt                                           <  6:337066/135‑1 (MQ=255)
                                                                                       ttGACGCCCCCCGCCCGGCCGCGCGCGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTCCCTCGACGCCGCCGAGCGCCTCa                                         <  5:58004/135‑1 (MQ=255)
                                                                                         gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAg                                        >  1:127804/1‑134 (MQ=255)
                                                                                         gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGcc                                      >  8:112077/1‑136 (MQ=255)
                                                                                         gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                       >  6:376475/1‑135 (MQ=255)
                                                                                         gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                       >  4:346460/1‑135 (MQ=255)
                                                                                         gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTGGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGGTCCTCGCCGCCGCCGCGCGCCTCAGc                                       >  2:254462/1‑135 (MQ=255)
                                                                                         gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCAGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                       >  8:263973/1‑135 (MQ=255)
                                                                                          ccgCCGACGGCCCGGCCGCGGGGGCCGCGCGGGGCGGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                                     <  3:372188/134‑1 (MQ=255)
                                                                                            gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTTGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCAGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                                    >  5:104499/1‑135 (MQ=255)
                                                                                            gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                                    >  8:233264/1‑135 (MQ=255)
                                                                                            gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                                    >  4:186246/1‑135 (MQ=255)
                                                                                            gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                                    >  3:296199/1‑135 (MQ=255)
                                                                                            gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                                    >  8:100671/1‑135 (MQ=255)
                                                                                            gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                                    >  7:54480/1‑135 (MQ=255)
                                                                                              cGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaa                                    >  1:5224/1‑133 (MQ=255)
                                                                                               gACCGCCCGGCCGCGCGGGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAg                                <  4:127423/136‑1 (MQ=255)
                                                                                                   gCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGCTGCCGGGCCGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGCGCGCCTCAGCCAACAAGTGc                             >  5:190645/1‑135 (MQ=255)
                                                                                                   gCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGcc                            >  8:206217/1‑136 (MQ=255)
                                                                                                     cgggCCGCGCGGGCGGGGGGGGGCCGGGGCTCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCgg                          <  7:338548/134‑1 (MQ=255)
                                                                                                           gggggggCCGCGCGGGGCCGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc                    <  3:17672/130‑1 (MQ=255)
                                                                                                           gcgcgTGGCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc                    <  4:234714/136‑1 (MQ=255)
                                                                                                            ggcgTGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc                    <  1:323049/134‑1 (MQ=255)
                                                                                                                 gCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGCGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCg              >  7:305716/1‑136 (MQ=255)
                                                                                                                 gCCGCGCGGGGCCGGGGCGAGGGGCCGGGGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTc               <  5:89224/135‑1 (MQ=255)
                                                                                                                       ctgggcctgggcGAGGGGCCGGTGGTGTCGTTGATGCCGGGCAGCCTGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTg         <  6:151816/135‑1 (MQ=255)
                                                                                                                             ctgggcGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTGCCCTgcg  >  3:337012/1‑136 (MQ=255)
                                                                                                                              tgggcGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGGTCCTCGACGCCGCCGAGCGCCTCCGGCATCAAGTGCCGGGTGTGCGCTACGTGTTGCCGTgag  >  5:196472/1‑133 (MQ=255)
                                                                                                                              |                                                                                                                                       
CGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTGCCCTGCG  >  ACB122/1791558‑1791818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: