Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,806,928 G→T 88.2% G76V (GGG→GTG)  yeiG → S‑formylglutathione hydrolase YeiG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,806,9280GT88.2% 235.9 / 19.6 96G76V (GGG→GTG) yeiGS‑formylglutathione hydrolase YeiG
Reads supporting (aligned to +/- strand):  ref base G (8/4);  new base T (47/37);  total (55/41)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

GCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACT  >  ACB122/1806798‑1807059
                                                                                                                                  |                                                                                                                                   
gCTGTGTACCTGCCGCCGCAGGCCGCGCAGGGCGAAAAGCTGACGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGACCTTCATGCTGAAGGCCGGCGCCCAGCGTCTGGCCGCCGCGCTGGTGCt                                                                                                                                  >  2:386102/1‑134 (MQ=255)
gCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTg                                                                                                                                 >  5:63797/1‑135 (MQ=255)
gCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGa                                                                                                                                >  7:95713/1‑136 (MQ=255)
gCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTg                                                                                                                                 >  5:56439/1‑135 (MQ=255)
     gtACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGatcatc                                                                                                                           <  4:41437/136‑1 (MQ=255)
       aCCTGCCGCCGCTGGCCGAGCCGGGCGAAAAGCGGCCGGTGCGGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGACGGCCGGCGCCCAGCGGCTGGCCGCCGAGCTGGTGCTGATCAtcg                                                                                                                          <  2:354250/135‑1 (MQ=255)
       aCCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCAtcgt                                                                                                                         >  4:209463/1‑136 (MQ=255)
       aCCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCAtcgt                                                                                                                         >  7:389203/1‑136 (MQ=255)
       aCCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCAtcg                                                                                                                          >  2:126593/1‑135 (MQ=255)
       aCCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGAGCAtcg                                                                                                                          >  1:148658/1‑135 (MQ=255)
       aCCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGCCGGCCGAGCTGGTGCTGATCAtcg                                                                                                                          >  6:382922/1‑135 (MQ=255)
        ccTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCAtcgtc                                                                                                                        >  7:114372/1‑136 (MQ=255)
        ccTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCAtcgt                                                                                                                         >  1:51264/1‑135 (MQ=255)
            ccgccgCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGc                                                                                                                      <  3:283365/134‑1 (MQ=255)
             cgccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGc                                                                                                                      <  6:180890/133‑1 (MQ=255)
               ccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCCGCGTCTGGCCGCCGAGCTGGTGCTGAACATCGTCGcccc                                                                                                                   >  2:149873/1‑134 (MQ=255)
               ccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGccccc                                                                                                                  >  3:290467/1‑135 (MQ=255)
               ccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGcccc                                                                                                                   >  1:26559/1‑134 (MQ=255)
               ccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGccccc                                                                                                                  >  4:179111/1‑135 (MQ=255)
                 gcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTTTACTGGCTCAGCGGCCTCACCTGCCCCGACGAGAACTTCATGCAGAAGGCCGGCGCCCCGCGTCTGGCCGCCGAGCTGGTGCTGCTCATCGTCGCCCCCg                                                                                                                 >  6:242638/1‑134 (MQ=255)
                 gcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCg                                                                                                                 >  6:57667/1‑134 (MQ=255)
                   aGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATAc                                                                                                             >  4:40627/1‑136 (MQ=255)
                   aGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATAc                                                                                                             >  8:285992/1‑136 (MQ=255)
                             gggCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCCCCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGt                                                                                                   >  7:333592/1‑136 (MQ=255)
                                  aaaaGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGc                                                                                                >  8:238841/1‑134 (MQ=255)
                                          ccGGTGCGGTCCTGGCTCCGCGGCCTCACCTGCACCGACGAGAACTTCAGGCAGAAGGCCGGCGCCCAGCGGCTGGCCGCCGAGCAGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGc                                                                                      <  7:87634/136‑1 (MQ=255)
                                           cGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGcc                                                                                     <  4:55884/136‑1 (MQ=255)
                                             gTGCCGTACGGGCTCCGCGGCCTCACCTCCACCGCCTAGAACTTCATGCCGACGGCCGGCGCCCAGCGTCGGGCCGCCGAGCGGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCg                                                                                    <  1:372565/135‑1 (MQ=255)
                                                     cgggCTCAGCGGCCTCACCTCCACCGACGAGAACTTCATGCAGACGGCCGGCGCCCAGCGTCGGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATcc                                                                            <  8:148143/133‑1 (MQ=255)
                                                        gCTCCGCGGCCGCACCTGCACCGACGAGACCTTCATGCAGAAGGCCGGCGCCCAGCGGCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCgg                                                                          <  7:182672/134‑1 (MQ=255)
                                                           cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGatcat                                                                                                                            >  1:135089/1‑81 (MQ=255)
                                                           cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGatcat                                                                                                                            <  2:135089/81‑1 (MQ=255)
                                                           cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGCGCAGGTGCCGGGCGGTCCGGACgg                                                                      >  5:232270/1‑135 (MQ=255)
                                                           cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  2:283203/1‑135 (MQ=255)
                                                           cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  1:232895/1‑135 (MQ=255)
                                                           cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  5:277264/1‑135 (MQ=255)
                                                           cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  6:35554/1‑135 (MQ=255)
                                                           cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  1:273357/1‑135 (MQ=255)
                                                           cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  1:29062/1‑135 (MQ=255)
                                                                gCCTCACCTGCCCCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGCCGGCGCCTg                                                                >  8:370430/1‑136 (MQ=255)
                                                                gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCcgcg                                                                                                    >  2:209871/1‑100 (MQ=255)
                                                                gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCcgcg                                                                                                    <  1:209871/100‑1 (MQ=255)
                                                                gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGCTCCGGACGGCGCCt                                                                 >  5:244002/1‑135 (MQ=255)
                                                                gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCt                                                                 >  1:215181/1‑135 (MQ=255)
                                                                gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTg                                                                >  7:322399/1‑136 (MQ=255)
                                                                gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTg                                                                >  8:99961/1‑136 (MQ=255)
                                                                gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCt                                                                 >  6:331437/1‑135 (MQ=255)
                                                                gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCCAGCACGTGCCGGGCGATCCCGACCGCGcca                                                                 >  2:195178/1‑134 (MQ=255)
                                                                   tCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGa                                                             >  8:251191/1‑136 (MQ=255)
                                                                     aCCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGCGCAGGTGCCGGGCGATCCGGACGGCGCCTgggcc                                                            >  1:321794/1‑133 (MQ=255)
                                                                         gCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCCAGCAGCTGCCGGGCGATCCGGACGGCGCCTGGGCCTTCg                                                        >  6:226729/1‑135 (MQ=255)
                                                                             cgacgaGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCt                                                    >  5:261088/1‑135 (MQ=255)
                                                                             cgacgaGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTg                                                   >  4:12431/1‑136 (MQ=255)
                                                                              gacgaGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTgg                                                  <  4:359084/136‑1 (MQ=255)
                                                                               tcgaGAACTGCATGCCGCAGGCCGGCGCCCCGCGTCGGGCCGCCGAGCTGGTGCTGATCATCGGCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTgg                                                  <  6:56439/134‑1 (MQ=255)
                                                                               acgaGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTggg                                                 >  4:280098/1‑136 (MQ=255)
                                                                                cgaGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGgc                                                <  7:99961/136‑1 (MQ=255)
                                                                                  agaACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGgcgc                                              >  4:164765/1‑136 (MQ=255)
                                                                                  agaACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGgcgc                                              >  3:262629/1‑136 (MQ=255)
                                                                                  agaACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGgcgc                                              >  3:330944/1‑136 (MQ=255)
                                                                                  agaACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACAGccc                                                                                                       >  8:75411/1‑78 (MQ=255)
                                                                                  agaACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACAGccc                                                                                                       <  7:75411/78‑1 (MQ=255)
                                                                                  agaACTTCATGCAGAAGGCCGGAGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGgcgc                                              >  7:341264/1‑136 (MQ=255)
                                                                                    aaCTTCATGCCGACGGCCGGCGCCCAGCGGCGGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCt                                             <  6:244002/135‑1 (MQ=255)
                                                                                      cTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGc                                          <  3:12431/136‑1 (MQ=255)
                                                                                      cTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGc                                          <  8:114372/136‑1 (MQ=255)
                                                                                       ttcattcaGAAGGCCGGCGCCCAGCGGCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGc                                          <  2:234815/135‑1 (MQ=255)
                                                                                       ttCATGCAGAAGGCCGGCGCCCAGCGGCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGc                                          <  2:112955/135‑1 (MQ=255)
                                                                                            gCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTa                                     >  2:143655/1‑135 (MQ=255)
                                                                                            gCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTa                                     >  2:255262/1‑135 (MQ=255)
                                                                                                   gCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAAc                              <  5:394699/135‑1 (MQ=255)
                                                                                                   gCCCGCGCCCAGCCTCAGGCCCCCGAGCTGGTTCTGATCATCGTCCCCCCCGAAACCAGCCCCCGTGGCGAGCACGTGCCGGGCGATCCCGACGGCGCCTGGGACTTCGGCCTTGGCCCTGGCCTCTACCACAACg                             >  8:264604/1‑136 (MQ=255)
                                                                                                    cgggCGCCCAGGTTCTGGCCGCCGAGCTGGTGCGGATCATCGTCGCCACAGATACCAGCCCGCGTGGCGATCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACg                             <  5:338822/133‑1 (MQ=255)
                                                                                                     cGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGcc                           >  7:155883/1‑136 (MQ=255)
                                                                                                        cgcCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCAcc                        <  4:262629/136‑1 (MQ=255)
                                                                                                        cgcCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCAcc                        <  8:155883/136‑1 (MQ=255)
                                                                                                         gcCCAGCGGCGGGCCGCCGAGCGGGTGCTGATCATCGGCGCCCCCGATACCAGCCCGCGGGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCAcc                        <  1:97687/135‑1 (MQ=255)
                                                                                                         gcCCAGCGCCGGGCCGCCGAGCTGGTGCGGATCATCGGCCCCCCCGAAACCAGCCCGCGTGGCGAGCAGGGGCCGGGCGATCCGGACGGCGCCCGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCAcc                        <  5:45972/135‑1 (MQ=255)
                                                                                                           ccAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCGGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCa                      >  2:165982/1‑135 (MQ=255)
                                                                                                             aGCGTCTGGCCGCCGAGCTGGTGCTGATCACCGTCGCCCCCGATCCCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCa                   <  7:216185/136‑1 (MQ=255)
                                                                                                                gTCAGGCCGCCCAGCTGGTGCTGATGATCGTCGCCCCCGAAAACATCCCGCCTTGAGAGCAGGTGCCCGGCGATACGGACGGCGCCTGGGCCTTCGGCCAGGGCGCTGGCTTCTACCTCAACGCCACCCCCCAAcc                >  4:204826/1‑136 (MQ=255)
                                                                                                                   tGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCt              <  5:180946/135‑1 (MQ=255)
                                                                                                                    ggCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTg             >  2:11219/1‑135 (MQ=255)
                                                                                                                     gccgccGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTgg            <  1:126593/135‑1 (MQ=255)
                                                                                                                     gccgccGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTgg            <  6:261088/135‑1 (MQ=255)
                                                                                                                     gccgccGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTgg            <  2:51264/135‑1 (MQ=255)
                                                                                                                      ccgccgAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCt              <  1:255262/132‑1 (MQ=255)
                                                                                                                        gccgAGCTGGTGCTGATCATCGTCGCCCCCGATACCCGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGcc         <  5:57667/135‑1 (MQ=255)
                                                                                                                        gccgAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGcc         <  2:321794/135‑1 (MQ=255)
                                                                                                                           gAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAg      <  6:63797/135‑1 (MQ=255)
                                                                                                                              cTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACt  <  3:40627/136‑1 (MQ=255)
                                                                                                                              cTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACt  <  8:389203/136‑1 (MQ=255)
                                                                                                                              cTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACt  <  3:179111/136‑1 (MQ=255)
                                                                                                                               tGGTGCTGTTGATGGTCTCCCCCGATAACTGCCGGGTTGGCGAGCAGGTGACGGGCGATCCGGACGGGGCCTGGGACTTGGGCCTGGGCGCTGTCTTCTACCTCAACGCCACCCAGCAAGCCTGGGCGGAGCACt  <  2:148658/135‑1 (MQ=255)
                                                                                                                               tGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACt  <  5:35554/135‑1 (MQ=255)
                                                                                                                               tGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACt  <  2:232895/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                   
GCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACT  >  ACB122/1806798‑1807059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: