Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,837,156	C→A	12.5%	G232C (GGC→TGC)	barA ←	Signal transduction histidine‑protein kinaseBarA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,837,156	0	C	A	12.5%	105.7 / 7.9	46	G232C (GGC→TGC)	barA	Signal transduction histidine‑protein kinaseBarA
Reads supporting (aligned to +/- strand):  ref base C (20/20);  new base A (4/2);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01

GTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATGGTGCGGCTCATGCGCAGCGCCA  >  ACB122/1837031‑1837290                                                                                                                              |                                                                                                                                       gTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGt                                                                                                                              >  8:68538/1‑136 (MQ=255)     aGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGGCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTc                                                                                                                            <  1:378774/134‑1 (MQ=255)          gATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCAATGCGGTTGATGCCGTGGGCCCGTTCGTCCAg                                                                                                                      >  2:365341/1‑135 (MQ=255)              ttCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCCGTTCGTCCAGCTCa                                                                                                                  >  5:4937/1‑135 (MQ=255)               tCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCAt                                                                                                                 >  1:46728/1‑135 (MQ=255)               tCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATg                                                                                                                >  4:284091/1‑136 (MQ=255)                    atgtCGATGGTTTCCAGGTGCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGGTGCAGTTCTTCGTGGGCATTCTGCAGGGTGTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGc                                                                                                           <  3:271052/133‑1 (MQ=255)                    aTTTCGCTGGGTTCCAGGGTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCAGTGGGCCAGTTCGTCCAGCTCATGGCTGc                                                                                                           <  4:206560/136‑1 (MQ=255)                         gATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCAt                                                                                                       >  2:294652/1‑135 (MQ=255)                           tGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCAATGGt                                                                                                    >  7:284923/1‑136 (MQ=255)                            ggTGTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGGTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTc                                                                                                   <  4:20414/136‑1 (MQ=255)                                             gcACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCAGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGa                                                                                   >  6:34594/1‑135 (MQ=255)                                               aCGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTGTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGt                                                                                <  3:33505/136‑1 (MQ=255)                                                cGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGt                                                                                <  6:63484/135‑1 (MQ=255)                                                              tGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCAGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCtt                                                                      >  6:50021/1‑131 (MQ=255)                                                              tGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCAGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCtt                                                                      <  5:50021/131‑1 (MQ=255)                                                                gTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGa                                                               >  7:216105/1‑136 (MQ=255)                                                                     tGCTGGGTTGCAGGTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGa                                                           <  6:215113/135‑1 (MQ=255)                                                                        tgtgTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGa                                                           <  2:47262/132‑1 (MQ=255)                                                                        tgtgTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGa                                                           >  1:47262/1‑132 (MQ=255)                                                                                 gtgcttcGTGGGCATTCTGCAGGGGTGCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGAt                                              <  3:268961/133‑1 (MQ=255)                                                                                  ttcttcGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGa                                                           <  5:203234/122‑1 (MQ=255)                                                                                  ttcttcGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGa                                                           >  6:203234/1‑122 (MQ=255)                                                                                  ttcttcGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGAcc                                                         <  7:66401/124‑1 (MQ=255)                                                                                  ttcttcGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGAcc                                                         >  8:66401/1‑124 (MQ=255)                                                                                  ttcttcGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGAt                                              >  2:102565/1‑135 (MQ=255)                                                                                  ttcttcGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATg                                             >  3:341882/1‑136 (MQ=255)                                                                                  ttcttcGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATg                                             >  3:305675/1‑136 (MQ=255)                                                                                     ttcGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGt                                          >  3:88025/1‑136 (MQ=255)                                                                                       cGTGGGCATTCTGCAGGGTGTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGtt                                         <  8:61239/135‑1 (MQ=255)                                                                                         tGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCg                                       <  1:102565/135‑1 (MQ=255)                                                                                            gCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGAt                                     <  5:207538/134‑1 (MQ=255)                                                                                                  tGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCg                                       >  5:314243/1‑126 (MQ=255)                                                                                                  tGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCg                                       <  6:314243/126‑1 (MQ=255)                                                                                                   gCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGt                             >  2:77142/1‑135 (MQ=255)                                                                                                    cAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGt                             <  8:216105/134‑1 (MQ=255)                                                                                                    cAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGGCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGt                             <  3:294448/134‑1 (MQ=255)                                                                                                       ggTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGttgttg                        >  3:171589/1‑136 (MQ=255)                                                                                                        gTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATgg                       <  3:284091/136‑1 (MQ=255)                                                                                                         tttCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCCTGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGGGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATgg                       <  5:13247/135‑1 (MQ=255)                                                                                                         tttCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATgg                       <  1:77142/135‑1 (MQ=255)                                                                                                          ttCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTGCGAGGTGGCCGTCCTTGAGCTCATTGCCCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATGGTg                     >  7:237754/1‑136 (MQ=255)                                                                                                               ccATGCGGATGATGCAGTGCGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCAGTCCTTGAGCTGATTCACCGCGTGCTTGATGCGTTCGATGGGGTCGTGGATGGTGCGGCt                >  8:119755/1‑136 (MQ=255)                                                                                                                    cGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGAGGTGTATGGTTCGGCTCATg            >  2:365553/1‑135 (MQ=255)                                                                                                                     ggTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTGCGAGGTGGCCGGCCTTGAGCTGACTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATGGTGCGGCTCATgcg          <  7:200732/136‑1 (MQ=255)                                                                                                                     ggTTGATGCAGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGCCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATGGTGCGGCGCATgcg          >  3:178410/1‑136 (MQ=255)                                                                                                                             ccGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGAT‑GGGTCGTTGATGGTGCGGCTCATGCGCAGCGCCa  <  1:294652/135‑1 (MQ=255)                                                                                                                              |                                                                                                                                       GTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATGGTGCGGCTCATGCGCAGCGCCA  >  ACB122/1837031‑1837290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑